Origin IGS: tagtctgtaaacaactatggagcgctactgttaggttaggtcctattttgcctatccttttttaaatcatttctaccccgattaaatatttaaagtcagtggttctcccttaatattaggaagaacaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttgttcttcctaatattaagggagaaccactgactttaaatatttaatcggggtagaaatgatttaaaaaaggataggcaaaataggacctaacctaacagtagcgctccatagttgtttacagacta Mask Tandem Repeat Region ================================================ tagtctgtaaacaactatggagcgctactgttaggttaggtcctattttgcctatccttttttaaatcatttctaccccgattaaatatttaaagtcagtggttctcccttaatattaggaagaacaa Find is-nt database================================================ Query_seq: AA02293:AA02294|AA02293:AA02294:hypothetical protein:conserved hypothetical protein:->->:1563770..1563897 128 tagtctgtaaacaactatggagcgctactgttaggttaggtcctattttgcctatccttttttaaatcatttctaccccgattaaatatttaaagtcagtggttctcccttaatattaggaagaacaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02293:AA02294|AA02293:AA02294:hypothetical protein:conserved hypothetical protein:->->:1563770..1563897 128 tagtctgtaaacaactatggagcgctactgttaggttaggtcctattttgcctatccttttttaaatcatttctaccccgattaaatatttaaagtcagtggttctcccttaatattaggaagaacaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02293:AA02294|AA02293:AA02294:hypothetical protein:conserved hypothetical protein:->->:1563770..1563897 128 tagtctgtaaacaactatggagcgctactgttaggttaggtcctattttgcctatccttttttaaatcatttctaccccgattaaatatttaaagtcagtggttctcccttaatattaggaagaacaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagtctgtaaacaactatggagcgctactgttaggttaggtcctattttgcctatccttttttaaatcatttctaccccgattaaatatttaaagtcagtggttctcccttaatattaggaagaacaa Predict ORF larger than 30AA ================================================ tagtctgtaaacaactatggagcgctactgttaggttaggtcctattttgcctatccttttttaaatcatttctaccccgattaaatatttaaagtcagtggttctcccttaatattaggaagaacaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagtctgtaaacaactatggagcgctactgttaggttaggtcctattttgcctatccttttttaaatcatttctaccccgattaaatatttaaagtcagtggttctcccttaatattaggaagaacaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02293:AA02294|AA02293:AA02294:hypothetical protein:conserved hypothetical protein:->->:1563770..1563897 128 tagtctgtaaacaactatggagcgctactgttaggttaggtcctattttgcctatccttttttaaatcatttctaccccgattaaatatttaaagtcagtggttctcccttaatattaggaagaacaa Intra-Species Hit: Count: 1 Min: 1 Max: 128 Len: 128 Subject: aact_AA02293_AA02294|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1563770,1563897]|128 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:1 Query End:128 Subject Strand: POSITIVE Subject Start: 1 Subject End: 128 tagtctgtaaacaactatggagcgctactgttaggttaggtcctattttgcctatccttttttaaatcatttctaccccgattaaatatttaaagtcagtggttctcccttaatattaggaagaacaa tagtctgtaaacaactatggagcgctactgttaggttaggtcctattttgcctatccttttttaaatcatttctaccccgattaaatatttaaagtcagtggttctcccttaatattaggaagaacaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUCUGUAAACAACUAUGGAGCGCUACUGUUAGGUUAGGUCCUAUUUUGCCUAUCCUUUUUUAAAUCAUUUCUACCCCGAUUAAAUAUUUAAAGUCAGUGGUUCUCCCUUAAUAUUAGGAAGAACAA ((((.((....)))))).((((.(((((((..(((.(((((........))))).))).((((((((....((......))......)))))))).))))))).)))).................... (-25.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUGUUCUUCCUAAUAUUAAGGGAGAACCACUGACUUUAAAUAUUUAAUCGGGGUAGAAAUGAUUUAAAAAAGGAUAGGCAAAAUAGGACCUAACCUAACAGUAGCGCUCCAUAGUUGUUUACAGACUA ..(((((((((.......))))))))).((((..(((((((......((......))....)))))))..(((.(((((.......).)))).)))..)))).........(((((.......))))) (-24.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 318 56 13 189 154 aact:AA01259|5end_protoporphyrinogen_oxidase_854354..854584_POSITIVE 293 57 15 167 122 aact:AA01255|5end_hypothetical_protein_852656..852886_POSITIVE 262 58 15 222 162 aact:AA02387|5end_integral_membrane_transport_protein_1634813..1635043_POSITIVE 262 52 12 159 113 aact:AA01355|5end_large_subunit_pseudouridylate_synthase_D_917228..917458_POSITIVE 260 55 15 203 163 aact:AA01209|5end_conserved_hypothetical_protein_826430..826660_POSITIVE 260 55 15 61 21 aact:AA01207|5end_hypothetical_protein_826288..826518_POSITIVE 249 60 12 104 45 aact:AA01349|5end_NAD(P)H_nitroreductase/H-flavin_oxidoreductase_913619..913849_POSITIVE 246 41 3 123 85 aact:AA01779|5end_molybdenum_ABC_transporter,_ATP-binding_protein_1200036..1200266_POSITIVE 243 59 11 173 125 aact:AA01191|5end_AMP-binding_enzyme_816000..816230_POSITIVE 242 38 1 222 184 aact:AA00047|5end_formate_dependent_nitrite_reductase,_cytochrome_c552_36753..36983_POSITIVE 241 59 16 211 170 aact:AA01168|5end_6-phosphogluconolactonase_801243..801473_POSITIVE 240 60 19 188 151 aact:AA02949|5end_hypothetical_protein_2061944..2062174_POSITIVE 240 58 11 152 109 aact:AA00822|5end_conserved_hypothetical_protein_(possible_arsenate_reductase)_564596..564826_POSITIVE 239 54 12 227 183 aact:AA02337|5end_DNA_mismatch_repair_protein_1597350..1597580_POSITIVE 237 45 3 117 78 aact:AA00425|5end_N-terminal_region_of_competence_protein_M_287295..287525_POSITIVE 236 120 76 209 176 aact:AA01568|5end_dipeptide_transport_system_ATP-binding_protein_1056037..1056267_POSITIVE 235 58 17 42 1 aact:AA01145|5end_glycyl-tRNA_synthetase_alpha_subunit_783478..783708_POSITIVE 234 42 3 49 8 aact:AA02486|5end_topoisomerase_IV,_subunit_B_1724337..1724567_POSITIVE 232 52 13 167 135 aact:AA00804|5end_fumarate_reductase_flavoprotein_subunit_A_549770..550000_POSITIVE 229 46 4 221 183 aact:AA02463|5end_N-acetylglucosamine-6-phosphate_deacetylase_1702804..1703034_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 293 57 15 133 88 aact:AA01254|3end_conserved_hypothetical_protein_(possible_proline_dipeptidase)_852702..852852_POSITIVE 265 58 18 59 1 aact:AA02386|3end_hypothetical_protein_1634730..1634880_POSITIVE 249 60 12 104 45 aact:AA01348|3end_hypothetical_protein_913699..913849_POSITIVE 242 41 11 149 122 aact:AA01190|3end_glycosyl_transferase_(possible_dolichol-phosphate_mannosyltransferase)_816077..816227_POSITIVE 242 38 1 145 107 aact:AA00046|3end_formate_dependent_nitrite_reductase,_cytochrome-c_type_protein_36756..36906_POSITIVE 240 60 19 38 1 aact:AA02947|3end_riboflavin_biosynthesis_protein_2061874..2062024_POSITIVE 237 45 3 145 106 aact:AA00424|3end_hypothetical_protein_287403..287553_POSITIVE 232 52 13 108 76 aact:AA00803|3end_hypothetical_protein_549791..549941_POSITIVE 231 69 22 125 66 aact:AA00543|3end_ADP-heptose--LPS_heptosyltransferase_II_372407..372557_POSITIVE 228 57 19 147 111 aact:AA02548|3end_desulfoviridin_gamma_subunit_1775849..1775999_POSITIVE 227 44 19 37 14 aact:AA02872|3end_conserved_hypothetical_protein_2016182..2016332_POSITIVE 227 56 19 73 30 aact:AA00776|3end_conserved_hypothetical_protein_533869..534019_POSITIVE 225 82 44 129 89 aact:AA01514|3end_peroxiredoxin_2_family_protein/glutaredoxin_1020695..1020845_POSITIVE 221 54 17 134 98 aact:AA02000|3end_2,3,4,5-tetrahydropyridine-2-carboxylate_1359801..1359951_POSITIVE 221 83 43 125 88 aact:AA01718|3end_DNA_polymerase_III,_chi_subunit_1163316..1163466_POSITIVE 220 57 17 106 65 aact:AA02416|3end_conserved_hypothetical_protein_1659578..1659728_POSITIVE 219 44 1 73 26 aact:AA01169|3end_hypothetical_protein_802229..802379_POSITIVE 218 58 16 143 97 aact:AA01679|3end_aspartate-semialdehyde_dehydrogenase;_PTS_system_enzyme_II_A_component_1135236..1135386_POSITIVE 215 58 13 150 87 aact:AA00084|3end_aminotransferase_62105..62255_POSITIVE 214 80 31 102 56 aact:AA00718|3end_ABC-related_ATP-binding_protein;_MRP_protein_494723..494873_POSITIVE