Origin IGS: aatttatcttcattttgcaatgttattgtatattaaggctggcatttcgcttttcaagcccgaatatcataaataaaaaacccgtcttcatacatactgaagatggtttgaaagaatcacaaccaaagtgcggtcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagaccgcactttggttgtgattctttcaaaccatcttcagtatgtatgaagacgggttttttatttatgatattcgggcttgaaaagcgaaatgccagccttaatatacaataacattgcaaaatgaagataaatt Mask Tandem Repeat Region ================================================ aatttatcttcattttgcaatgttattgtatattaaggctggcatttcgcttttcaagcccgaatatcataaataaaaaacccgtcttcatacatactgaagatggtttgaaagaatcacaaccaaagtgcggtcta Find is-nt database================================================ Query_seq: AA02290:AA02291|AA02290:AA02291:cell division protein J; ribosomal RNA large subunit methyltransferase J:formyltetrahydrofolate deformylase:->->:1562049..1562185 137 aatttatcttcattttgcaatgttattgtatattaaggctggcatttcgcttttcaagcccgaatatcataaataaaaaacccgtcttcatacatactgaagatggtttgaaagaatcacaaccaaagtgcggtcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02290:AA02291|AA02290:AA02291:cell division protein J; ribosomal RNA large subunit methyltransferase J:formyltetrahydrofolate deformylase:->->:1562049..1562185 137 aatttatcttcattttgcaatgttattgtatattaaggctggcatttcgcttttcaagcccgaatatcataaataaaaaacccgtcttcatacatactgaagatggtttgaaagaatcacaaccaaagtgcggtcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02290:AA02291|AA02290:AA02291:cell division protein J; ribosomal RNA large subunit methyltransferase J:formyltetrahydrofolate deformylase:->->:1562049..1562185 137 aatttatcttcattttgcaatgttattgtatattaaggctggcatttcgcttttcaagcccgaatatcataaataaaaaacccgtcttcatacatactgaagatggtttgaaagaatcacaaccaaagtgcggtcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatttatcttcattttgcaatgttattgtatattaaggctggcatttcgcttttcaagcccgaatatcataaataaaaaacccgtcttcatacatactgaagatggtttgaaagaatcacaaccaaagtgcggtcta Predict ORF larger than 30AA ================================================ Protein_Len: 44 Strand: + Start: 6 End: 137 ..... M F I L Q C Y C I L R L A F R F S S P N I I N K K P V F I H T E D G L K E S Q P K C G L aatttatcttcattttgcaatgttattgtatattaaggctggcatttcgcttttcaagcccgaatatcataaataaaaaacccgtcttcatacatactgaagatggtttgaaagaatcacaaccaaagtgcggtcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatttatcttcattttgcaatgttattgtatattaaggctggcatttcgcttttcaagcccgaatatcataaataaaaaacccgtcttcatacatactgaagatggtttgaaagaatcacaaccaaagtgcggtcta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 62 HP_score: -6.5 Tail_Score: -3.54424 Start: 82 End: 106 Full_Region: TCATAAATAAAAAAC CCGTCTTCA TACATAC TGAAGATGG TTTGAAAGAATCACA .................................................................................CCGTCTTCATACATACTGAAGATGG............................... Find igs database================================================ Query_seq: AA02290:AA02291|AA02290:AA02291:cell division protein J; ribosomal RNA large subunit methyltransferase J:formyltetrahydrofolate deformylase:->->:1562049..1562185 137 aatttatcttcattttgcaatgttattgtatattaaggctggcatttcgcttttcaagcccgaatatcataaataaaaaacccgtcttcatacatactgaagatggtttgaaagaatcacaaccaaagtgcggtcta Intra-Species Hit: Count: 1 Min: 1 Max: 137 Len: 137 Subject: aact_AA02290_AA02291|cell division protein J; ribosomal RNA large subunit methyltransferase J:formyltetrahydrofolate deformylase|POSITIVE:POSITIVE|[1562049,1562185]|137 HSP 1 e-value: 3.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: POSITIVE Subject Start: 1 Subject End: 137 aatttatcttcattttgcaatgttattgtatattaaggctggcatttcgcttttcaagcccgaatatcataaataaaaaacccgtcttcatacatactgaagatggtttgaaagaatcacaaccaaagtgcggtcta aatttatcttcattttgcaatgttattgtatattaaggctggcatttcgcttttcaagcccgaatatcataaataaaaaacccgtcttcatacatactgaagatggtttgaaagaatcacaaccaaagtgcggtcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUUUAUCUUCAUUUUGCAAUGUUAUUGUAUAUUAAGGCUGGCAUUUCGCUUUUCAAGCCCGAAUAUCAUAAAUAAAAAACCCGUCUUCAUACAUACUGAAGAUGGUUUGAAAGAAUCACAACCAAAGUGCGGUCUA .......((.(((((((...(((..(((((((((..(((((((.....))).....))))..))))).)))).........(((((((((.......)))))))))............)))..))))))).)).... (-27.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGACCGCACUUUGGUUGUGAUUCUUUCAAACCAUCUUCAGUAUGUAUGAAGACGGGUUUUUUAUUUAUGAUAUUCGGGCUUGAAAAGCGAAAUGCCAGCCUUAAUAUACAAUAACAUUGCAAAAUGAAGAUAAAUU .(((((......(((((.(((.....))))))))((((((.......))))))..)))))(((((((.(((((((.(((((.(....((.....)))))))).))))).))....((((....)))).))))))).. (-26.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 295 64 35 218 184 aact:AA00551|5end_hypothetical_protein_377225..377455_POSITIVE 287 70 36 194 158 aact:AA02736|5end_conserved_hypothetical_protein_1917350..1917580_POSITIVE 282 49 5 79 40 aact:AA00468|5end_tryptophan-specific_transport_protein_/_tyrosine-specific_transport_protein_324803..325033_POSITIVE 272 68 38 66 43 aact:AA02538|5end_conserved_hypothetical_protein_1765187..1765417_POSITIVE 262 61 35 35 6 aact:AA02683|5end_hydrogenase_4_Fe-S_subunit_1868882..1869112_POSITIVE 260 70 36 91 67 aact:AA01876|5end_PTS_system,_IIBC_components_1263365..1263595_POSITIVE 258 70 35 55 24 aact:AA00454|5end_conserved_hypothetical_protein_318020..318250_POSITIVE 257 62 28 145 97 aact:AA01079|5end_conserved_hypothetical_protein_747404..747634_POSITIVE 256 68 36 207 168 aact:AA01563|5end_transketolase_isozyme_1_1046969..1047199_POSITIVE 255 60 16 61 18 aact:AA00310|5end_conserved_hypothetical_protein_210193..210423_POSITIVE 252 50 4 101 61 aact:AA02529|5end_cytidylate_kinase_1_1757713..1757943_POSITIVE 250 70 21 228 176 aact:AA02516|5end_ferric_uptake_regulation_protein_1749264..1749494_POSITIVE 250 47 6 231 189 aact:AA00018|5end_hypothetical_protein_10550..10780_POSITIVE 248 60 13 67 18 aact:AA00475|5end_conserved_hypothetical_protein_(possible_N6-adenine-specific_methylase)_331621..331851_POSITIVE 247 60 18 229 170 aact:AA01345|5end_conserved_hypothetical_protein_(possible_membrane_protein)_912624..912854_POSITIVE 245 74 36 173 128 aact:AA02933|5end_conserved_hypothetical_protein_(HesA/MoeB/ThiF_family_protein)_2053467..2053697_POSITIVE 239 70 38 98 68 aact:AA02969|5end_beta_tyrosinase_(tyrosine_phenol-lyase)_2076107..2076337_POSITIVE 239 62 34 87 56 aact:AA02758|5end_3-phosphoshikimate_1-carboxyvinyltransferase_1931802..1932032_POSITIVE 239 59 14 164 121 aact:AA01903|5end_hypothetical_protein_1283983..1284213_POSITIVE 238 69 24 104 59 aact:AA00936|5end_ribose_ABC_transporter,_permease_protein,_C-terminal_646289..646519_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 295 64 35 114 80 aact:AA00550|3end_ferredoxin-type_protein_377201..377351_POSITIVE 282 49 5 74 35 aact:AA00467|3end_hypothetical_protein_324878..325028_POSITIVE 273 50 11 140 102 aact:AA02351|3end_conserved_hypothetical_protein_1609468..1609618_POSITIVE 272 68 38 62 39 aact:AA02536|3end_conserved_hypothetical_protein_1765263..1765413_POSITIVE 257 62 28 114 66 aact:AA01078|3end_transcriptional_regulator_(probable_MerR_family)_747453..747603_POSITIVE 256 68 36 129 90 aact:AA01561|3end_conserved_hypothetical_protein_1046971..1047121_POSITIVE 255 60 16 55 12 aact:AA00309|3end_3-hydroxyisobutyrate_dehydrogenase;_2-hydroxy-3-oxopropionate_reductase_210267..210417_POSITIVE 248 60 13 90 41 aact:AA00474|3end_cell_division_protein_Y_(GTP-binding_signal_recognition_particle)_331724..331874_POSITIVE 247 69 36 151 128 aact:AA01875|3end_PTS_system,_glucose-specific_enzyme_II,_A_component_1263506..1263656_POSITIVE 241 63 37 151 114 aact:AA00820|3end_succinyl-diaminopimelate_desuccinylase_564536..564686_POSITIVE 239 70 38 72 42 aact:AA02968|3end_hypothetical_protein_2076161..2076311_POSITIVE 238 69 24 63 18 aact:AA00935|3end_ribose_ABC_transporter,_permease_protein,_N-terminal_646328..646478_POSITIVE 237 90 48 127 80 aact:AA02254|3end_2-oxoglutarate/malate_translocator_1536380..1536530_POSITIVE 234 130 82 121 83 aact:AA02430|3end_conserved_hypothetical_protein_1675669..1675819_POSITIVE 234 63 30 54 10 aact:AA01304|3end_penicillin-binding_protein_1A_885580..885730_POSITIVE 227 59 12 125 82 aact:AA02632|3end_rhamnosyltransferase_1832694..1832844_POSITIVE 226 63 21 106 60 aact:AA01880|3end_N-acetylglucosamine_repressor_1268383..1268533_POSITIVE 226 69 27 137 101 aact:AA01209|3end_conserved_hypothetical_protein_827007..827157_POSITIVE 225 62 26 140 99 aact:AA02184|3end_anthranilate_isomerase;_indole-3-glycerol_phosphate_synthase;_phosphoribosylanthranilate_isomerase_1492541..1492691_POSITIVE 224 63 21 133 86 aact:AA01615|3end_lipoprotein_releasing_system_transmembrane_1092447..1092597_POSITIVE