Origin IGS: ataaatcacctatacgaaatatgttgaataaaatgattctaaccttgaattccactcaaattctataaaataccaatccattcatattttttgtgatccatttcaaataattaaaatttagctattctcgatattttgagttacgcatggcaattttcttaggtcatatttccgataataaccataaatgagtcaaaaaagcttaagcaaaaggctatatttgagtcaaaaatgcctcatttctattctttgactccgcttttcttattttgtattagtgctattgtacgtttgaatttcactttctgttcagtgaataactcatcaagcagtacctctcatacactcacatcgataaattaaggaattgatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aatcaattccttaatttatcgatgtgagtgtatgagaggtactgcttgatgagttattcactgaacagaaagtgaaattcaaacgtacaatagcactaatacaaaataagaaaagcggagtcaaagaatagaaatgaggcatttttgactcaaatatagccttttgcttaagcttttttgactcatttatggttattatcggaaatatgacctaagaaaattgccatgcgtaactcaaaatatcgagaatagctaaattttaattatttgaaatggatcacaaaaaatatgaatggattggtattttatagaatttgagtggaattcaaggttagaatcattttattcaacatatttcgtataggtgatttat Mask Tandem Repeat Region ================================================ ataaatcacctatacgaaatatgttgaataaaatgattctaaccttgaattccactcaaattctataaaataccaatccattcatattttttgtgatccatttcaaataattaaaatttagctattctcgatattttgagttacgcatggcaattttcttaggtcatatttccgataataaccataaatgagtcaaaaaagcttaagcaaaaggctatatttgagtcaaaaatgcctcatttctattctttgactccgcttttcttattttgtattagtgctattgtacgtttgaatttcactttctgttcagtgaataactcatcaagcagtacctctcatacactcacatcgataaattaaggaattgatt Find is-nt database================================================ Query_seq: AA02285:AA02286|AA02285:AA02286:phosphoglycerate transport regulatory protein:sugar ABC transporter, ATP-binding protein:->->:1557345..1557717 373 ataaatcacctatacgaaatatgttgaataaaatgattctaaccttgaattccactcaaattctataaaataccaatccattcatattttttgtgatccatttcaaataattaaaatttagctattctcgatattttgagttacgcatggcaattttcttaggtcatatttccgataataaccataaatgagtcaaaaaagcttaagcaaaaggctatatttgagtcaaaaatgcctcatttctattctttgactccgcttttcttattttgtattagtgctattgtacgtttgaatttcactttctgttcagtgaataactcatcaagcagtacctctcatacactcacatcgataaattaaggaattgatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02285:AA02286|AA02285:AA02286:phosphoglycerate transport regulatory protein:sugar ABC transporter, ATP-binding protein:->->:1557345..1557717 373 ataaatcacctatacgaaatatgttgaataaaatgattctaaccttgaattccactcaaattctataaaataccaatccattcatattttttgtgatccatttcaaataattaaaatttagctattctcgatattttgagttacgcatggcaattttcttaggtcatatttccgataataaccataaatgagtcaaaaaagcttaagcaaaaggctatatttgagtcaaaaatgcctcatttctattctttgactccgcttttcttattttgtattagtgctattgtacgtttgaatttcactttctgttcagtgaataactcatcaagcagtacctctcatacactcacatcgataaattaaggaattgatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02285:AA02286|AA02285:AA02286:phosphoglycerate transport regulatory protein:sugar ABC transporter, ATP-binding protein:->->:1557345..1557717 373 ataaatcacctatacgaaatatgttgaataaaatgattctaaccttgaattccactcaaattctataaaataccaatccattcatattttttgtgatccatttcaaataattaaaatttagctattctcgatattttgagttacgcatggcaattttcttaggtcatatttccgataataaccataaatgagtcaaaaaagcttaagcaaaaggctatatttgagtcaaaaatgcctcatttctattctttgactccgcttttcttattttgtattagtgctattgtacgtttgaatttcactttctgttcagtgaataactcatcaagcagtacctctcatacactcacatcgataaattaaggaattgatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ataaatcacctatacgaaatatgttgaataaaatgattctaaccttgaattccactcaaattctataaaataccaatccattcatattttttgtgatccatttcaaataattaaaatttagctattctcgatattttgagttacgcatggcaattttcttaggtcatatttccgataataaccataaatgagtcaaaaaagcttaagcaaaaggctatatttgagtcaaaaatgcctcatttctattctttgactccgcttttcttattttgtattagtgctattgtacgtttgaatttcactttctgttcagtgaataactcatcaagcagtacctctcatacactcacatcgataaattaaggaattgatt Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 166 End: 360 ..................................................................................................................................................................... M F P I I T I N E S K K L K Q K A I F E S K M P H F Y S L T P L F L F C I S A I V R L N F T F C S V N N S S S S T S H T ............. ataaatcacctatacgaaatatgttgaataaaatgattctaaccttgaattccactcaaattctataaaataccaatccattcatattttttgtgatccatttcaaataattaaaatttagctattctcgatattttgagttacgcatggcaattttcttaggtcatatttccgataataaccataaatgagtcaaaaaagcttaagcaaaaggctatatttgagtcaaaaatgcctcatttctattctttgactccgcttttcttattttgtattagtgctattgtacgtttgaatttcactttctgttcagtgaataactcatcaagcagtacctctcatacactcacatcgataaattaaggaattgatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ataaatcacctatacgaaatatgttgaataaaatgattctaaccttgaattccactcaaattctataaaataccaatccattcatattttttgtgatccatttcaaataattaaaatttagctattctcgatattttgagttacgcatggcaattttcttaggtcatatttccgataataaccataaatgagtcaaaaaagcttaagcaaaaggctatatttgagtcaaaaatgcctcatttctattctttgactccgcttttcttattttgtattagtgctattgtacgtttgaatttcactttctgttcagtgaataactcatcaagcagtacctctcatacactcacatcgataaattaaggaattgatt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02285:AA02286|AA02285:AA02286:phosphoglycerate transport regulatory protein:sugar ABC transporter, ATP-binding protein:->->:1557345..1557717 373 ataaatcacctatacgaaatatgttgaataaaatgattctaaccttgaattccactcaaattctataaaataccaatccattcatattttttgtgatccatttcaaataattaaaatttagctattctcgatattttgagttacgcatggcaattttcttaggtcatatttccgataataaccataaatgagtcaaaaaagcttaagcaaaaggctatatttgagtcaaaaatgcctcatttctattctttgactccgcttttcttattttgtattagtgctattgtacgtttgaatttcactttctgttcagtgaataactcatcaagcagtacctctcatacactcacatcgataaattaaggaattgatt Intra-Species Hit: Count: 1 Min: 1 Max: 373 Len: 373 Subject: aact_AA02285_AA02286|phosphoglycerate transport regulatory protein:sugar ABC transporter, ATP-binding protein|POSITIVE:POSITIVE|[1557345,1557717]|373 HSP 1 e-value: 0.0 bit: 739.0 Len: 373 Query Start:1 Query End:373 Subject Strand: POSITIVE Subject Start: 1 Subject End: 373 ataaatcacctatacgaaatatgttgaataaaatgattctaaccttgaattccactcaaattctataaaataccaatccattcatattttttgtgatccatttcaaataattaaaatttagctattctcgatattttgagttacgcatggcaattttcttaggtcatatttccgataataaccataaatgagtcaaaaaagcttaagcaaaaggctatatttgagtcaaaaatgcctcatttctattctttgactccgcttttcttattttgtattagtgctattgtacgtttgaatttcactttctgttcagtgaataactcatcaagcagtacctctcatacactcacatcgataaattaaggaattgatt ataaatcacctatacgaaatatgttgaataaaatgattctaaccttgaattccactcaaattctataaaataccaatccattcatattttttgtgatccatttcaaataattaaaatttagctattctcgatattttgagttacgcatggcaattttcttaggtcatatttccgataataaccataaatgagtcaaaaaagcttaagcaaaaggctatatttgagtcaaaaatgcctcatttctattctttgactccgcttttcttattttgtattagtgctattgtacgtttgaatttcactttctgttcagtgaataactcatcaagcagtacctctcatacactcacatcgataaattaaggaattgatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAAAUCACCUAUACGAAAUAUGUUGAAUAAAAUGAUUCUAACCUUGAAUUCCACUCAAAUUCUAUAAAAUACCAAUCCAUUCAUAUUUUUUGUGAUCCAUUUCAAAUAAUUAAAAUUUAGCUAUUCUCGAUAUUUUGAGUUACGCAUGGCAAUUUUCUUAGGUCAUAUUUCCGAUAAUAACCAUAAAUGAGUCAAAAAAGCUUAAGCAAAAGGCUAUAUUUGAGUCAAAAAUGCCUCAUUUCUAUUCUUUGACUCCGCUUUUCUUAUUUUGUAUUAGUGCUAUUGUACGUUUGAAUUUCACUUUCUGUUCAGUGAAUAACUCAUCAAGCAGUACCUCUCAUACACUCACAUCGAUAAAUUAAGGAAUUGAUU ...((((((((....((((((((..((((......))))..((((.((((((((...........................(((((.....)))))........................((....(((((....)))))....)).))).)))))....))))))))))))((((.........(((((((....((((((...(((.....)))......((((((((((((.........)))).)))))))).)))))))))))))(((((.((.(.((((((.....(((..((((((........))))))....)))....)))))).).)).))))).....))))........)))...))))) (-63.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUCAAUUCCUUAAUUUAUCGAUGUGAGUGUAUGAGAGGUACUGCUUGAUGAGUUAUUCACUGAACAGAAAGUGAAAUUCAAACGUACAAUAGCACUAAUACAAAAUAAGAAAAGCGGAGUCAAAGAAUAGAAAUGAGGCAUUUUUGACUCAAAUAUAGCCUUUUGCUUAAGCUUUUUUGACUCAUUUAUGGUUAUUAUCGGAAAUAUGACCUAAGAAAAUUGCCAUGCGUAACUCAAAAUAUCGAGAAUAGCUAAAUUUUAAUUAUUUGAAAUGGAUCACAAAAAAUAUGAAUGGAUUGGUAUUUUAUAGAAUUUGAGUGGAAUUCAAGGUUAGAAUCAUUUUAUUCAACAUAUUUCGUAUAGGUGAUUUAU ..((((......(((((((((((((((((.((((...((((..((((..((((((..(((((........)))))))))))...((......))..................)))).(((((((((((.((....(((((((.((((...)))).)).)))))...)).....))))))))))).((((.((((((..........)))))))))).....))))...)))))))))...)))))).......))))))........)))).(((((((((...(((((((((((((.((((..((((.....(((((((...)))))))..)))))))).))))))))...))))).......))))))))) (-72.45) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 278 250 209 121 80 aact:AA02921|5end_conserved_hypothetical_protein_(cell_filamentation_protein)_2045674..2045904_POSITIVE 272 176 137 59 16 aact:AA02973|5end_tyrosine-specific_transport_protein_2077707..2077937_POSITIVE 272 259 212 225 176 aact:AA00782|5end_conserved_hypothetical_protein_538723..538953_POSITIVE 267 190 143 157 103 aact:AA00426|5end_conserved_hypothetical_protein_288027..288257_POSITIVE 263 349 303 217 165 aact:AA01286|5end_5,10-methylenetetrahydrofolate_dehydrogenase_(bifunctional_protein)_870309..870539_POSITIVE 260 295 255 133 95 aact:AA02693|5end_hydrogenase-4_component_H_1877921..1878151_POSITIVE 254 164 121 198 156 aact:AA00083|5end_hypothetical_protein_60664..60894_POSITIVE 245 166 124 45 2 aact:AA01830|5end_2,3-cyclic-nucleotide_2-phosphodiesterase_1231556..1231786_POSITIVE 245 358 311 66 24 aact:AA00429|5end_conserved_hypothetical_protein_290567..290797_POSITIVE 241 317 279 182 145 aact:AA01349|5end_NAD(P)H_nitroreductase/H-flavin_oxidoreductase_913619..913849_POSITIVE 241 317 279 57 20 aact:AA01348|5end_hypothetical_protein_913494..913724_POSITIVE 236 280 233 205 160 aact:AA01272|5end_hypothetical_protein_864039..864269_POSITIVE 233 170 121 194 147 aact:AA01253|5end_hypothetical_protein_852189..852419_POSITIVE 233 248 207 49 9 aact:AA00325|5end_conserved_hypothetical_protein_221314..221544_POSITIVE 232 236 201 212 180 aact:AA00845|5end_hypothetical_protein_584531..584761_POSITIVE 231 288 244 229 176 aact:AA02735|5end_DNA_repair_protein_1915974..1916204_POSITIVE 229 179 134 169 127 aact:AA00012|5end_conserved_hypothetical_protein_6433..6663_POSITIVE 228 368 328 92 51 aact:AA01569|5end_dipeptide_transport_ATP-binding_protein_1056859..1057089_POSITIVE 227 317 288 195 155 aact:AA02288|5end_conserved_hypothetical_protein_1558590..1558820_POSITIVE 225 355 328 214 190 aact:AA02142|5end_outer_membrane_protein_OprM_precursor_1466037..1466267_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 278 250 209 116 75 aact:AA02920|3end_hypothetical_protein_2045749..2045899_POSITIVE 267 190 143 123 69 aact:AA00425|3end_N-terminal_region_of_competence_protein_M_288073..288223_POSITIVE 263 349 303 122 70 aact:AA01284|3end_heat_shock_protein_(hsp60)_870294..870444_POSITIVE 260 295 255 116 78 aact:AA02692|3end_hydrogenase_4_subunit;_hydrogenase_3_subunit_1877984..1878134_POSITIVE 245 166 124 71 28 aact:AA01829|3end_hypothetical_protein_1231662..1231812_POSITIVE 245 260 213 124 86 aact:AA00977|3end_transcriptional_regulator_(LysR_family)_683334..683484_POSITIVE 241 317 279 62 25 aact:AA01347|3end_conserved_hypothetical_protein_913579..913729_POSITIVE 239 280 231 110 56 aact:AA02931|3end_UDP-N-acetylmuramate:L-alanyl-gamma-d-glutamayl-m_edo-diaminopimelate_2052046..2052196_POSITIVE 239 238 200 136 102 aact:AA00419|3end_conserved_hypothetical_protein_(possible_phosphatidylethanolamine-binding_protein)_284911..285061_POSITIVE 236 280 233 67 22 aact:AA01271|3end_hypothetical_protein_863981..864131_POSITIVE 232 236 201 144 112 aact:AA00844|3end_cell_division_protein_I;_penicillin_binding_protein_2X_584543..584693_POSITIVE 228 368 328 85 44 aact:AA01568|3end_dipeptide_transport_system_ATP-binding_protein_1056932..1057082_POSITIVE 223 130 85 140 82 aact:AA01668|3end_transcription_elongation_factor_1130383..1130533_POSITIVE 222 340 296 105 66 aact:AA02980|3end_conserved_hypothetical_protein_2084245..2084395_POSITIVE 219 158 127 131 95 aact:AA00641|3end_cell_division_inhibitor_440130..440280_POSITIVE 217 335 298 108 72 aact:AA00354|3end_hypothetical_protein_234862..235012_POSITIVE 216 259 211 63 11 aact:AA02947|3end_riboflavin_biosynthesis_protein_2061874..2062024_POSITIVE 216 260 211 115 63 aact:AA02544|3end_ATP-dependent_RNA_helicase_1773945..1774095_POSITIVE 216 166 126 116 80 aact:AA01565|3end_conserved_hypothetical_protein_1050967..1051117_POSITIVE 215 350 301 129 87 aact:AA01152|3end_sec-independent_protein_translocase_protein_790205..790355_POSITIVE