Origin IGS: taaaaaagtgcggttagaaaacgccatggattttgaccgcacttttcgatttataatttataacttttcttatccaacccatattttttcatgcgtaagtaaagtttctttctgggaatattgaggtgtttaactacgtcattaattttccccta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taggggaaaattaatgacgtagttaaacacctcaatattcccagaaagaaactttacttacgcatgaaaaaatatgggttggataagaaaagttataaattataaatcgaaaagtgcggtcaaaatccatggcgttttctaaccgcactttttta Mask Tandem Repeat Region ================================================ taaaaaagtgcggttagaaaacgccatggattttgaccgcacttttcgatttataatttataacttttcttatccaacccatattttttcatgcgtaagtaaagtttctttctgggaatattgaggtgtttaactacgtcattaattttccccta Find is-nt database================================================ Query_seq: AA02280:AA02281|AA02280:AA02281:conserved hypothetical protein:phosphoglycerate transport system activator protein:->->:1552828..1552982 155 taaaaaagtgcggttagaaaacgccatggattttgaccgcacttttcgatttataatttataacttttcttatccaacccatattttttcatgcgtaagtaaagtttctttctgggaatattgaggtgtttaactacgtcattaattttccccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02280:AA02281|AA02280:AA02281:conserved hypothetical protein:phosphoglycerate transport system activator protein:->->:1552828..1552982 155 taaaaaagtgcggttagaaaacgccatggattttgaccgcacttttcgatttataatttataacttttcttatccaacccatattttttcatgcgtaagtaaagtttctttctgggaatattgaggtgtttaactacgtcattaattttccccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02280:AA02281|AA02280:AA02281:conserved hypothetical protein:phosphoglycerate transport system activator protein:->->:1552828..1552982 155 taaaaaagtgcggttagaaaacgccatggattttgaccgcacttttcgatttataatttataacttttcttatccaacccatattttttcatgcgtaagtaaagtttctttctgggaatattgaggtgtttaactacgtcattaattttccccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaaaagtgcggttagaaaacgccatggattttgaccgcacttttcgatttataatttataacttttcttatccaacccatattttttcatgcgtaagtaaagtttctttctgggaatattgaggtgtttaactacgtcattaattttccccta Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: + Start: 8 End: 121 ....... M R L E N A M D F D R T F R F I I Y N F S Y P T H I F S C V S K V S F W E Y .................................. Protein_Len: 31 Strand: - Start: 54 End: 146 ..................................................... L K Y S K K D L G Y K K M R L Y L K K R P I N L H K V V D N M ......... taaaaaagtgcggttagaaaacgccatggattttgaccgcacttttcgatttataatttataacttttcttatccaacccatattttttcatgcgtaagtaaagtttctttctgggaatattgaggtgtttaactacgtcattaattttccccta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaaaagtgcggttagaaaacgccatggattttgaccgcacttttcgatttataatttataacttttcttatccaacccatattttttcatgcgtaagtaaagtttctttctgggaatattgaggtgtttaactacgtcattaattttccccta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02280:AA02281|AA02280:AA02281:conserved hypothetical protein:phosphoglycerate transport system activator protein:->->:1552828..1552982 155 taaaaaagtgcggttagaaaacgccatggattttgaccgcacttttcgatttataatttataacttttcttatccaacccatattttttcatgcgtaagtaaagtttctttctgggaatattgaggtgtttaactacgtcattaattttccccta Intra-Species Hit: Count: 1 Min: 1 Max: 155 Len: 155 Subject: aact_AA02280_AA02281|conserved hypothetical protein:phosphoglycerate transport system activator protein|POSITIVE:POSITIVE|[1552828,1552982]|155 HSP 1 e-value: 6.0E-83 bit: 307.0 Len: 155 Query Start:1 Query End:155 Subject Strand: POSITIVE Subject Start: 1 Subject End: 155 taaaaaagtgcggttagaaaacgccatggattttgaccgcacttttcgatttataatttataacttttcttatccaacccatattttttcatgcgtaagtaaagtttctttctgggaatattgaggtgtttaactacgtcattaattttccccta taaaaaagtgcggttagaaaacgccatggattttgaccgcacttttcgatttataatttataacttttcttatccaacccatattttttcatgcgtaagtaaagtttctttctgggaatattgaggtgtttaactacgtcattaattttccccta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAAAGUGCGGUUAGAAAACGCCAUGGAUUUUGACCGCACUUUUCGAUUUAUAAUUUAUAACUUUUCUUAUCCAACCCAUAUUUUUUCAUGCGUAAGUAAAGUUUCUUUCUGGGAAUAUUGAGGUGUUUAACUACGUCAUUAAUUUUCCCCUA ...((((((((((((((((..(.....)..))))))))))))))))...............((((((.(((((.((...............)).))))).)))))).......(((((.((((((..((......))..).))))).)))))... (-38.36) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGGGAAAAUUAAUGACGUAGUUAAACACCUCAAUAUUCCCAGAAAGAAACUUUACUUACGCAUGAAAAAAUAUGGGUUGGAUAAGAAAAGUUAUAAAUUAUAAAUCGAAAAGUGCGGUCAAAAUCCAUGGCGUUUUCUAACCGCACUUUUUUA ...(((((...((.(((.((........)).))).))))))).......((((((.(((((.((((......)))).)).....))).))))))..............((((((((((((.((((((....).)))))...)))))))))))).. (-30.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 582 45 3 212 170 aact:AA01625|5end_riboflavin_synthase,_beta_chain_1099964..1100194_POSITIVE 567 45 3 141 99 aact:AA00126|5end_conserved_hypothetical_protein_91053..91283_POSITIVE 544 47 4 56 12 aact:AA00697|5end_hypothetical_protein_476999..477229_POSITIVE 499 48 4 216 171 aact:AA00541|5end_conserved_hypothetical_protein_370140..370370_POSITIVE 499 48 4 126 81 aact:AA00540|5end_hypothetical_protein_370050..370280_POSITIVE 489 50 5 192 146 aact:AA02893|5end_oligopeptide_transport_system_permease_protein,_N-terminal_2027201..2027431_POSITIVE 487 45 4 135 94 aact:AA00091|5end_short-patch_reverse_transcription_protein_67741..67971_POSITIVE 482 45 1 156 112 aact:AA00473|5end_tryptophanyl-tRNA_synthetase_328996..329226_POSITIVE 474 46 5 206 165 aact:AA02754|5end_conserved_hypothetical_protein_1928949..1929179_POSITIVE 462 48 4 123 78 aact:AA02362|5end_conserved_hypothetical_protein_1619905..1620135_POSITIVE 458 46 5 155 114 aact:AA02113|5end_conserved_hypothetical_protein_1442183..1442413_POSITIVE 456 46 5 169 128 aact:AA01544|5end_conserved_hypothetical_protein_1034265..1034495_POSITIVE 456 46 5 79 38 aact:AA01543|5end_hypothetical_protein_1034175..1034405_POSITIVE 455 46 1 82 37 aact:AA02378|5end_hypothetical_protein_1627753..1627983_POSITIVE 454 45 2 213 169 aact:AA01145|5end_glycyl-tRNA_synthetase_alpha_subunit_783478..783708_POSITIVE 450 49 5 126 83 aact:AA01673|5end_riboflavin_synthase,_alpha_chain_1131477..1131707_POSITIVE 441 46 4 75 32 aact:AA02037|5end_hypothetical_protein_1386832..1387062_POSITIVE 431 48 4 223 174 aact:AA02778|5end_hypothetical_protein_1942585..1942815_POSITIVE 431 46 4 76 32 aact:AA02002|5end_hypothetical_protein_1360196..1360426_POSITIVE 427 50 3 152 107 aact:AA02010|5end_peptide_methionine_sulfoxide_reductase;_cytochrome_C-TYPE_biogenesis_protein;_transcriptional_regulator_1367180..1367410_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 582 45 3 69 27 aact:AA01624|3end_glucose-6-phosphate_isomerase_1099901..1100051_POSITIVE 544 47 4 151 107 aact:AA00697|3end_hypothetical_protein_477174..477324_POSITIVE 544 47 4 83 39 aact:AA00696|3end_iron(III)_ABC_transporter,_periplasmic_iron-compound-binding_protein_477106..477256_POSITIVE 542 50 4 60 16 aact:AA00010|3end_transposase_orfB_fragment_6297..6447_POSITIVE 489 50 5 88 42 aact:AA02892|3end_oligopeptide_ABC_transporter,_periplasmic-binding_protein_2027177..2027327_POSITIVE 487 45 4 63 22 aact:AA00089|3end_S-adenosylmethionine_synthase;_methionine_adenosyltransferase_67749..67899_POSITIVE 482 45 1 63 19 aact:AA00471|3end_3,4-dihydroxy-2-butanone_4-phosphate_synthase;_GTP_cyclohydrase_II_328983..329133_POSITIVE 474 46 5 45 4 aact:AA02751|3end_L-lactate_permease_1928868..1929018_POSITIVE 472 48 7 43 1 aact:AA00539|3end_ADP-L-glycero-D-manno-heptose-6-epimerase_370047..370197_POSITIVE 466 46 3 79 36 aact:AA02781|3end_protein-export_membrane_protein_1945981..1946131_POSITIVE 462 48 4 85 40 aact:AA02360|3end_threonine_synthase_1619947..1620097_POSITIVE 458 46 5 76 35 aact:AA02112|3end_cell_division_protein_1442184..1442334_POSITIVE 457 46 4 87 45 aact:AA02280|3end_conserved_hypothetical_protein_1552767..1552917_POSITIVE 450 49 5 87 44 aact:AA01672|3end_conserved_hypothetical_protein_1131518..1131668_POSITIVE 449 49 4 82 35 aact:AA00218|3end_argininosuccinate_lyase_152211..152361_POSITIVE 441 46 4 68 25 aact:AA02035|3end_translation_initiation_factor_IF-3_1386905..1387055_POSITIVE 439 48 3 56 7 aact:AA02208|3end_tryptophan_synthase,__alpha_subunit_1503996..1504146_POSITIVE 431 48 4 85 36 aact:AA02777|3end_tRNA-guanine_transglycosylase_1942527..1942677_POSITIVE 430 50 4 81 37 aact:AA01922|3end_translation_initiation_factor_IF2_1297279..1297429_POSITIVE 425 48 4 83 35 aact:AA00896|3end_prolyl-tRNA_synthetase_(proline-tRNA_ligase)_623206..623356_POSITIVE