Origin IGS: tattaatcatcctataaaatttgtttaagcgattcaaaattgaatgcgggtgggattctaacagagcattccggcatttagaatagctaacacttgtacgcctaacttgtccgatcagttattttttcatggaaatagcgattta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaatcgctatttccatgaaaaaataactgatcggacaagttaggcgtacaagtgttagctattctaaatgccggaatgctctgttagaatcccacccgcattcaattttgaatcgcttaaacaaattttataggatgattaata Mask Tandem Repeat Region ================================================ tattaatcatcctataaaatttgtttaagcgattcaaaattgaatgcgggtgggattctaacagagcattccggcatttagaatagctaacacttgtacgcctaacttgtccgatcagttattttttcatggaaatagcgattta Find is-nt database================================================ Query_seq: AA02269:AA02270|AA02269:AA02270:3-hydroxydecanoyl-(acyl-carrier protein) dehydratase:endopeptidase La; lon protease; protease La homolog; ATP-dependent protease LA (lon-1):->->:1546865..1547009 145 tattaatcatcctataaaatttgtttaagcgattcaaaattgaatgcgggtgggattctaacagagcattccggcatttagaatagctaacacttgtacgcctaacttgtccgatcagttattttttcatggaaatagcgattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02269:AA02270|AA02269:AA02270:3-hydroxydecanoyl-(acyl-carrier protein) dehydratase:endopeptidase La; lon protease; protease La homolog; ATP-dependent protease LA (lon-1):->->:1546865..1547009 145 tattaatcatcctataaaatttgtttaagcgattcaaaattgaatgcgggtgggattctaacagagcattccggcatttagaatagctaacacttgtacgcctaacttgtccgatcagttattttttcatggaaatagcgattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02269:AA02270|AA02269:AA02270:3-hydroxydecanoyl-(acyl-carrier protein) dehydratase:endopeptidase La; lon protease; protease La homolog; ATP-dependent protease LA (lon-1):->->:1546865..1547009 145 tattaatcatcctataaaatttgtttaagcgattcaaaattgaatgcgggtgggattctaacagagcattccggcatttagaatagctaacacttgtacgcctaacttgtccgatcagttattttttcatggaaatagcgattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tattaatcatcctataaaatttgtttaagcgattcaaaattgaatgcgggtgggattctaacagagcattccggcatttagaatagctaacacttgtacgcctaacttgtccgatcagttattttttcatggaaatagcgattta Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: - Start: 2 End: 118 . N I M R Y F K N L R N L I S H P H S E L L A N R C K S Y S V S T R R V Q G I M ........................... tattaatcatcctataaaatttgtttaagcgattcaaaattgaatgcgggtgggattctaacagagcattccggcatttagaatagctaacacttgtacgcctaacttgtccgatcagttattttttcatggaaatagcgattta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tattaatcatcctataaaatttgtttaagcgattcaaaattgaatgcgggtgggattctaacagagcattccggcatttagaatagctaacacttgtacgcctaacttgtccgatcagttattttttcatggaaatagcgattta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02269:AA02270|AA02269:AA02270:3-hydroxydecanoyl-(acyl-carrier protein) dehydratase:endopeptidase La; lon protease; protease La homolog; ATP-dependent protease LA (lon-1):->->:1546865..1547009 145 tattaatcatcctataaaatttgtttaagcgattcaaaattgaatgcgggtgggattctaacagagcattccggcatttagaatagctaacacttgtacgcctaacttgtccgatcagttattttttcatggaaatagcgattta Intra-Species Hit: Count: 1 Min: 1 Max: 145 Len: 145 Subject: aact_AA02269_AA02270|3-hydroxydecanoyl-(acyl-carrier protein) dehydratase:endopeptidase La; lon protease; protease La homolog; ATP-dependent protease LA (lon-1)|POSITIVE:POSITIVE|[1546865,1547009]|145 HSP 1 e-value: 5.0E-77 bit: 287.0 Len: 145 Query Start:1 Query End:145 Subject Strand: POSITIVE Subject Start: 1 Subject End: 145 tattaatcatcctataaaatttgtttaagcgattcaaaattgaatgcgggtgggattctaacagagcattccggcatttagaatagctaacacttgtacgcctaacttgtccgatcagttattttttcatggaaatagcgattta tattaatcatcctataaaatttgtttaagcgattcaaaattgaatgcgggtgggattctaacagagcattccggcatttagaatagctaacacttgtacgcctaacttgtccgatcagttattttttcatggaaatagcgattta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUUAAUCAUCCUAUAAAAUUUGUUUAAGCGAUUCAAAAUUGAAUGCGGGUGGGAUUCUAACAGAGCAUUCCGGCAUUUAGAAUAGCUAACACUUGUACGCCUAACUUGUCCGAUCAGUUAUUUUUUCAUGGAAAUAGCGAUUUA .....................((((..(((.((((.....(((((((.((...(.(((.....))))...)).))))))))))).)))))))....................((((.(((((((((....))))))))))))).. (-25.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUCGCUAUUUCCAUGAAAAAAUAACUGAUCGGACAAGUUAGGCGUACAAGUGUUAGCUAUUCUAAAUGCCGGAAUGCUCUGUUAGAAUCCCACCCGCAUUCAAUUUUGAAUCGCUUAAACAAAUUUUAUAGGAUGAUUAAUA ..(((((.....(((((((((...(((((.........))))).........((...(((.((((.((((....((((((..................)))))).)))).)))).)))...))...)))))).)))))))).... (-24.07) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 289 119 71 113 57 aact:AA01567|5end_conserved_hypothetical_protein_(possible_cell-wall-associated_hydrolase)_1054944..1055174_POSITIVE 289 119 71 113 57 aact:AA01262|5end_hydrolase_859576..859806_POSITIVE 286 75 38 206 160 aact:AA01859|5end_hypothetical_protein_1251718..1251948_POSITIVE 285 114 71 224 170 aact:AA01086|5end_hypothetical_protein_751392..751622_POSITIVE 284 87 41 114 56 aact:AA01858|5end_conserved_hypothetical_protein_1250699..1250929_POSITIVE 281 87 45 50 12 aact:AA00499|5end_pyridoxamine-5'-phosphate_oxidase_346570..346800_POSITIVE 280 79 31 93 46 aact:AA01325|5end_formamidopyrimidine-DNA_glycosylase_894747..894977_POSITIVE 279 79 31 93 49 aact:AA01747|5end_single-stranded-DNA-specific_exonuclease_1181359..1181589_POSITIVE 276 87 45 56 9 aact:AA00819|5end_D-alanyl-D-alanine_carboxypeptidase_562644..562874_POSITIVE 275 90 46 109 58 aact:AA01017|5end_conserved_hypothetical_protein_710915..711145_POSITIVE 274 140 93 48 1 aact:AA00329|5end_molybdopterin-guanine_dinucleotide_biosynthesis_protein_222737..222967_POSITIVE 274 79 39 55 8 aact:AA00190|5end_triosephosphate_isomerase_134088..134318_POSITIVE 271 88 41 166 107 aact:AA01066|5end_conserved_hypothetical_protein_738380..738610_POSITIVE 270 87 43 61 2 aact:AA02226|5end_ABC_transporter_ATP-binding_protein_1514502..1514732_POSITIVE 270 89 47 90 32 aact:AA00636|5end_MrsA_protein_(phosphoglucomutase/phosphomannomutase)_436780..437010_POSITIVE 269 76 41 163 129 aact:AA00201|5end_prolipoprotein_diacylglyceryl_transferase_139740..139970_POSITIVE 268 113 72 219 189 aact:AA01522|5end_conserved_hypothetical_protein_1022840..1023070_POSITIVE 265 75 31 214 172 aact:AA01525|5end_amino_acid_ABC_transporter,_permease_component_1024277..1024507_POSITIVE 265 132 100 141 110 aact:AA01056|5end_conserved_hypothetical_protein_733867..734097_POSITIVE 265 78 41 143 97 aact:AA01054|5end_conserved_hypothetical_protein_733678..733908_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 280 88 41 70 30 aact:AA00314|3end_conserved_hypothetical_protein_(possible_Kmt1_protein)_214326..214476_POSITIVE 279 79 31 50 6 aact:AA01746|3end_thiol:disulfide_interchange_protein_1181396..1181546_POSITIVE 273 89 44 98 56 aact:AA00987|3end_tetracenomycin_polyketide_synthesis_O-methyltransferase_690586..690736_POSITIVE 273 88 45 63 13 aact:AA00534|3end_hypothetical_protein_365594..365744_POSITIVE 271 88 41 98 39 aact:AA01064|3end_conserved_hypothetical_protein_(possible_aminomethyltransferase)_738392..738542_POSITIVE 270 87 43 71 12 aact:AA02225|3end_ABC_transporter_ATP-binding_protein_1514592..1514742_POSITIVE 269 76 41 112 78 aact:AA00200|3end_thymidylate_synthase_139769..139919_POSITIVE 267 58 18 135 102 aact:AA00168|3end_possible_sigma_54_modulation_protein_120240..120390_POSITIVE 265 132 100 134 103 aact:AA01054|3end_conserved_hypothetical_protein_733940..734090_POSITIVE 265 78 41 132 86 aact:AA01053|3end_conserved_hypothetical_protein_733747..733897_POSITIVE 263 74 36 60 23 aact:AA00908|3end_probable_sigma-E_factor_regulatory_protein_626590..626740_POSITIVE 261 76 44 65 37 aact:AA02700|3end_succinyl-CoA_synthase,_alpha_subunit_1884205..1884355_POSITIVE 261 55 11 43 6 aact:AA02101|3end_penicillin-binding_protein_7_1431188..1431338_POSITIVE 260 117 71 125 82 aact:AA02369|3end_conserved_hypothetical_protein_1624648..1624798_POSITIVE 259 85 48 86 49 aact:AA00176|3end_pantothenate_permease_126044..126194_POSITIVE 254 109 63 52 9 aact:AA00444|3end_NTP_binding_protein_309647..309797_POSITIVE 252 75 35 44 3 aact:AA02684|3end_hydrogenase_4_membrane_subunit_1871612..1871762_POSITIVE 252 90 43 131 70 aact:AA02145|3end_iron(III)_ABC_transporter,_ATP-binding_protein_1469176..1469326_POSITIVE 249 75 40 46 6 aact:AA02840|3end_cytochrome_D_ubiquinol_oxidase,_subunit_II_1994622..1994772_POSITIVE 247 88 48 131 77 aact:AA01227|3end_hypothetical_protein_838845..838995_POSITIVE