Origin IGS: tgaaaacgaccgcactttatttggcttttcatcttaacatcttgcgaagatagacatatgatggacaaaaggattacaaagcgtaagggattattcgcacgcacttaacattccgtctatcaacccgggaggtttcaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttgaaacctcccgggttgatagacggaatgttaagtgcgtgcgaataatcccttacgctttgtaatccttttgtccatcatatgtctatcttcgcaagatgttaagatgaaaagccaaataaagtgcggtcgttttca Mask Tandem Repeat Region ================================================ tgaaaacgaccgcactttatttggcttttcatcttaacatcttgcgaagatagacatatgatggacaaaaggattacaaagcgtaagggattattcgcacgcacttaacattccgtctatcaacccgggaggtttcaaa Find is-nt database================================================ Query_seq: AA02253:AA02254|AA02253:AA02254:hypothetical protein:2-oxoglutarate/malate translocator:->->:1534847..1534985 139 tgaaaacgaccgcactttatttggcttttcatcttaacatcttgcgaagatagacatatgatggacaaaaggattacaaagcgtaagggattattcgcacgcacttaacattccgtctatcaacccgggaggtttcaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02253:AA02254|AA02253:AA02254:hypothetical protein:2-oxoglutarate/malate translocator:->->:1534847..1534985 139 tgaaaacgaccgcactttatttggcttttcatcttaacatcttgcgaagatagacatatgatggacaaaaggattacaaagcgtaagggattattcgcacgcacttaacattccgtctatcaacccgggaggtttcaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02253:AA02254|AA02253:AA02254:hypothetical protein:2-oxoglutarate/malate translocator:->->:1534847..1534985 139 tgaaaacgaccgcactttatttggcttttcatcttaacatcttgcgaagatagacatatgatggacaaaaggattacaaagcgtaagggattattcgcacgcacttaacattccgtctatcaacccgggaggtttcaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgaaaacgaccgcactttatttggcttttcatcttaacatcttgcgaagatagacatatgatggacaaaaggattacaaagcgtaagggattattcgcacgcacttaacattccgtctatcaacccgggaggtttcaaa Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: - Start: 3 End: 104 .. F R G C K I Q S K M K V D Q S S L C I I S L L I V F R L P N N A R M ................................... tgaaaacgaccgcactttatttggcttttcatcttaacatcttgcgaagatagacatatgatggacaaaaggattacaaagcgtaagggattattcgcacgcacttaacattccgtctatcaacccgggaggtttcaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 83 Start: 33 End: 62 ................................CTTAACATCTTGCGAAGATAGACATATGAT............................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgaaaacgaccgcactttatttggcttttcatcttaacatcttgcgaagatagacatatgatggacaaaaggattacaaagcgtaagggattattcgcacgcacttaacattccgtctatcaacccgggaggtttcaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02253:AA02254|AA02253:AA02254:hypothetical protein:2-oxoglutarate/malate translocator:->->:1534847..1534985 139 tgaaaacgaccgcactttatttggcttttcatcttaacatcttgcgaagatagacatatgatggacaaaaggattacaaagcgtaagggattattcgcacgcacttaacattccgtctatcaacccgggaggtttcaaa Intra-Species Hit: Count: 1 Min: 1 Max: 139 Len: 139 Subject: aact_AA02253_AA02254|hypothetical protein:2-oxoglutarate/malate translocator|POSITIVE:POSITIVE|[1534847,1534985]|139 HSP 1 e-value: 2.0E-73 bit: 276.0 Len: 139 Query Start:1 Query End:139 Subject Strand: POSITIVE Subject Start: 1 Subject End: 139 tgaaaacgaccgcactttatttggcttttcatcttaacatcttgcgaagatagacatatgatggacaaaaggattacaaagcgtaagggattattcgcacgcacttaacattccgtctatcaacccgggaggtttcaaa tgaaaacgaccgcactttatttggcttttcatcttaacatcttgcgaagatagacatatgatggacaaaaggattacaaagcgtaagggattattcgcacgcacttaacattccgtctatcaacccgggaggtttcaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAAACGACCGCACUUUAUUUGGCUUUUCAUCUUAACAUCUUGCGAAGAUAGACAUAUGAUGGACAAAAGGAUUACAAAGCGUAAGGGAUUAUUCGCACGCACUUAACAUUCCGUCUAUCAACCCGGGAGGUUUCAAA (((((.(..(((...(((((((.((..................))..)))))))....((((((((....(((.....(((.((..(.((....)).)..)).))).....)))))))))))...)))..).))))).. (-28.27) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUGAAACCUCCCGGGUUGAUAGACGGAAUGUUAAGUGCGUGCGAAUAAUCCCUUACGCUUUGUAAUCCUUUUGUCCAUCAUAUGUCUAUCUUCGCAAGAUGUUAAGAUGAAAAGCCAAAUAAAGUGCGGUCGUUUUCA ..(((((.(..(((...((((.(((((((.((((...(((((.(........).)))))....))))..))))))).))))...((((..(.((....)).)...))))..................)))..).))))) (-27.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 350 45 1 93 43 aact:AA02091|5end_hypothetical_protein_1424441..1424671_POSITIVE 347 130 93 216 177 aact:AA00585|5end_glycerol-3-phosphate_regulon_repressor_399195..399425_POSITIVE 337 47 6 72 30 aact:AA01880|5end_N-acetylglucosamine_repressor_1267083..1267313_POSITIVE 331 116 72 227 178 aact:AA00253|5end_hypothetical_protein_177431..177661_POSITIVE 320 26 1 132 107 aact:AA02716|5end_peptidase_T_1899789..1900019_POSITIVE 313 46 3 174 136 aact:AA00386|5end_aspartate--ammonia_ligase_(asparagine_synthetase_A)_261150..261380_POSITIVE 312 45 1 148 101 aact:AA02106|5end_hypothetical_protein_1433241..1433471_POSITIVE 312 42 7 140 110 aact:AA01291|5end_excinuclease_ABC_subunit_A_872829..873059_POSITIVE 305 130 81 160 112 aact:AA01146|5end_conserved_hypothetical_protein_784433..784663_POSITIVE 303 28 2 131 104 aact:AA01537|5end_possible_glucokinase_regulatory_protein_1030616..1030846_POSITIVE 301 115 81 37 2 aact:AA02673|5end_gluconate_utilization_operon_repressor_1858913..1859143_POSITIVE 300 46 3 163 121 aact:AA02410|5end_cell_division_protein_1655429..1655659_POSITIVE 293 41 7 178 140 aact:AA00801|5end_lysine--tRNA_ligase_548494..548724_POSITIVE 290 130 81 65 22 aact:AA02206|5end_tryptophan_synthase,_beta_chain_1501917..1502147_POSITIVE 290 25 1 134 111 aact:AA00249|5end_cysteinyl-tRNA_synthetase_(cysteine--tRNA_ligase)_173966..174196_POSITIVE 290 25 1 36 13 aact:AA00248|5end_hypothetical_protein_173868..174098_POSITIVE 286 128 88 200 155 aact:AA00519|5end_O-acetyltransferase_357139..357369_POSITIVE 280 46 1 155 114 aact:AA00464|5end_conserved_hypothetical_protein_323912..324142_POSITIVE 279 45 1 159 111 aact:AA02369|5end_conserved_hypothetical_protein_1624248..1624478_POSITIVE 278 47 7 143 83 aact:AA01989|5end_hypothetical_protein_1352039..1352269_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 350 45 1 121 71 aact:AA02090|3end_glucose-1-phosphate_adenylyltransferase_1424549..1424699_POSITIVE 347 130 93 151 112 aact:AA00583|3end_GlpG_protein_399210..399360_POSITIVE 320 26 1 50 25 aact:AA02715|3end_paraquat-inducible_protein_pqiB_homolog_1899787..1899937_POSITIVE 313 46 3 112 74 aact:AA00384|3end_conserved_hypothetical_protein_(possible_nitrogen_fixation_protein)_261168..261318_POSITIVE 312 45 1 117 70 aact:AA02105|3end_killing_protein_1433290..1433440_POSITIVE 312 42 7 79 49 aact:AA01289|3end_hypothetical_protein_872848..872998_POSITIVE 305 130 81 107 59 aact:AA01145|3end_glycyl-tRNA_synthetase_alpha_subunit_784460..784610_POSITIVE 300 46 3 58 16 aact:AA02409|3end_DNA_ligase_1655404..1655554_POSITIVE 300 46 4 120 67 aact:AA01423|3end_cell_division_protein_X,_ABC_system_permease_957680..957830_POSITIVE 297 31 7 134 109 aact:AA02184|3end_anthranilate_isomerase;_indole-3-glycerol_phosphate_synthase;_phosphoribosylanthranilate_isomerase_1492541..1492691_POSITIVE 293 41 7 106 68 aact:AA00799|3end_iron(III)_dicitrate_transport_ATP-binding_protein_548502..548652_POSITIVE 290 130 81 52 9 aact:AA02205|3end_short_chain_dehydrogenase/reductase_1501984..1502134_POSITIVE 290 25 1 137 114 aact:AA00248|3end_hypothetical_protein_174049..174199_POSITIVE 290 25 1 34 11 aact:AA00247|3end_peptidyl-prolyl_cis-trans_isomerase_B_173946..174096_POSITIVE 289 130 81 133 81 aact:AA00927|3end_ATP-dependent_DNA_helicase_637777..637927_POSITIVE 280 46 1 61 20 aact:AA00463|3end_class_B_acid_phosphatase_323898..324048_POSITIVE 279 45 1 117 69 aact:AA02368|3end_conserved_hypothetical_protein_1624286..1624436_POSITIVE 279 47 1 150 105 aact:AA01937|3end_sugar_fermentation_stimulation_protein_1309334..1309484_POSITIVE 276 90 41 56 8 aact:AA01478|3end_hypothetical_protein_994780..994930_POSITIVE 275 32 1 88 55 aact:AA00209|3end_D-ribose_ABC_transporter,_periplasmic-binding_protein_146032..146182_POSITIVE