Origin IGS: ttcagactggctaagccaaaccgtctccttctcaaaagacacggttaacgaaaccttcccgtcctgactagtgtaaatttgtatttggttattcattgaattaatccttatgccataaaccgactcaatataatcagcaatagattatca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgataatctattgctgattatattgagtcggtttatggcataaggattaattcaatgaataaccaaatacaaatttacactagtcaggacgggaaggtttcgttaaccgtgtcttttgagaaggagacggtttggcttagccagtctgaa Mask Tandem Repeat Region ================================================ ttcagactggctaagccaaaccgtctccttctcaaaagacacggttaacgaaaccttcccgtcctgactagtgtaaatttgtatttggttattcattgaattaatccttatgccataaaccgactcaatataatcagcaatagattatca Find is-nt database================================================ Query_seq: AA02244:AA02245|AA02244:AA02245:conserved hypothetical protein (probable DNA-binding protein; death-on-curing family protein0:hypothetical protein:->->:1527436..1527585 150 ttcagactggctaagccaaaccgtctccttctcaaaagacacggttaacgaaaccttcccgtcctgactagtgtaaatttgtatttggttattcattgaattaatccttatgccataaaccgactcaatataatcagcaatagattatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02244:AA02245|AA02244:AA02245:conserved hypothetical protein (probable DNA-binding protein; death-on-curing family protein0:hypothetical protein:->->:1527436..1527585 150 ttcagactggctaagccaaaccgtctccttctcaaaagacacggttaacgaaaccttcccgtcctgactagtgtaaatttgtatttggttattcattgaattaatccttatgccataaaccgactcaatataatcagcaatagattatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02244:AA02245|AA02244:AA02245:conserved hypothetical protein (probable DNA-binding protein; death-on-curing family protein0:hypothetical protein:->->:1527436..1527585 150 ttcagactggctaagccaaaccgtctccttctcaaaagacacggttaacgaaaccttcccgtcctgactagtgtaaatttgtatttggttattcattgaattaatccttatgccataaaccgactcaatataatcagcaatagattatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttcagactggctaagccaaaccgtctccttctcaaaagacacggttaacgaaaccttcccgtcctgactagtgtaaatttgtatttggttattcattgaattaatccttatgccataaaccgactcaatataatcagcaatagattatca Predict ORF larger than 30AA ================================================ Protein_Len: 47 Strand: - Start: 1 End: 141 E S Q S L W V T E K E F S V T L S V K G D Q S T Y I Q I Q N N M S N I R I G Y V S E I Y D A M ......... ttcagactggctaagccaaaccgtctccttctcaaaagacacggttaacgaaaccttcccgtcctgactagtgtaaatttgtatttggttattcattgaattaatccttatgccataaaccgactcaatataatcagcaatagattatca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttcagactggctaagccaaaccgtctccttctcaaaagacacggttaacgaaaccttcccgtcctgactagtgtaaatttgtatttggttattcattgaattaatccttatgccataaaccgactcaatataatcagcaatagattatca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02244:AA02245|AA02244:AA02245:conserved hypothetical protein (probable DNA-binding protein; death-on-curing family protein0:hypothetical protein:->->:1527436..1527585 150 ttcagactggctaagccaaaccgtctccttctcaaaagacacggttaacgaaaccttcccgtcctgactagtgtaaatttgtatttggttattcattgaattaatccttatgccataaaccgactcaatataatcagcaatagattatca Intra-Species Hit: Count: 1 Min: 1 Max: 150 Len: 150 Subject: aact_AA02244_AA02245|conserved hypothetical protein (probable DNA-binding protein; death-on-curing family protein0:hypothetical protein|POSITIVE:POSITIVE|[1527436,1527585]|150 HSP 1 e-value: 5.0E-80 bit: 297.0 Len: 150 Query Start:1 Query End:150 Subject Strand: POSITIVE Subject Start: 1 Subject End: 150 ttcagactggctaagccaaaccgtctccttctcaaaagacacggttaacgaaaccttcccgtcctgactagtgtaaatttgtatttggttattcattgaattaatccttatgccataaaccgactcaatataatcagcaatagattatca ttcagactggctaagccaaaccgtctccttctcaaaagacacggttaacgaaaccttcccgtcctgactagtgtaaatttgtatttggttattcattgaattaatccttatgccataaaccgactcaatataatcagcaatagattatca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUCAGACUGGCUAAGCCAAACCGUCUCCUUCUCAAAAGACACGGUUAACGAAACCUUCCCGUCCUGACUAGUGUAAAUUUGUAUUUGGUUAUUCAUUGAAUUAAUCCUUAUGCCAUAAACCGACUCAAUAUAAUCAGCAAUAGAUUAUCA .((((((.(((...))).((((((.((..........)).))))))..............)).))))........(((((((..((((((((..(((((.........................)))))))))))))..))))))).... (-21.91) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUAAUCUAUUGCUGAUUAUAUUGAGUCGGUUUAUGGCAUAAGGAUUAAUUCAAUGAAUAACCAAAUACAAAUUUACACUAGUCAGGACGGGAAGGUUUCGUUAACCGUGUCUUUUGAGAAGGAGACGGUUUGGCUUAGCCAGUCUGAA .............(((((((((((((((..((((.........))))..)))))))).......((((....))))....)))))))..((((..((((..(((((((((.(((((....))))).)))).)))))..))))..)))).. (-35.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 346 46 1 230 176 aact:AA01086|5end_hypothetical_protein_751392..751622_POSITIVE 340 89 42 53 12 aact:AA00914|5end_aminoacyl-histidine_dipeptidase;_Beta-alanyl-histidine_dipeptidase_628038..628268_POSITIVE 329 69 21 130 73 aact:AA01565|5end_conserved_hypothetical_protein_1050711..1050941_POSITIVE 309 65 28 206 166 aact:AA02076|5end_conserved_hypothetical_protein_1409926..1410156_POSITIVE 305 82 41 198 159 aact:AA01201|5end_phosphatase;_hydrolase_822513..822743_POSITIVE 288 49 3 175 125 aact:AA02070|5end_hypothetical_protein_1406508..1406738_POSITIVE 280 50 1 181 133 aact:AA00796|5end_iron(III)_dicitrate_transport_system_permease_protein_545686..545916_POSITIVE 277 78 31 142 87 aact:AA02316|5end_exodeoxyribonuclease_VII_large_subunit_1581682..1581912_POSITIVE 277 56 14 178 132 aact:AA02032|5end_hypothetical_protein_1385080..1385310_POSITIVE 272 44 4 157 112 aact:AA01295|5end_3-dehydroquinate_synthase_877272..877502_POSITIVE 270 81 42 59 21 aact:AA01666|5end_penicillin-binding_protein_4;_D-alanyl-D-alanine_carboxypeptidase;_D-alanyl-D-alanine-endopeptidase_1128231..1128461_POSITIVE 265 66 21 67 16 aact:AA02035|5end_translation_initiation_factor_IF-3_1386349..1386579_POSITIVE 263 66 21 43 1 aact:AA01660|5end_hypothetical_protein_1125035..1125265_POSITIVE 259 58 14 153 111 aact:AA00989|5end_hypothetical_protein_692319..692549_POSITIVE 257 49 1 127 76 aact:AA01432|5end_lysophospholipase_L2_960715..960945_POSITIVE 256 84 41 198 156 aact:AA01080|5end_hypothetical_protein_747679..747909_POSITIVE 256 74 34 229 182 aact:AA00515|5end_hypothetical_protein_355730..355960_POSITIVE 256 74 34 108 61 aact:AA00514|5end_hypothetical_protein_355609..355839_POSITIVE 251 67 21 151 107 aact:AA02088|5end_glycogen_operon_protein_1421282..1421512_POSITIVE 251 56 13 193 149 aact:AA01779|5end_molybdenum_ABC_transporter,_ATP-binding_protein_1200036..1200266_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 305 82 41 91 52 aact:AA01199|3end_conserved_hypothetical_protein_822486..822636_POSITIVE 295 45 8 133 99 aact:AA01263|3end_hypothetical_protein_861086..861236_POSITIVE 288 49 3 118 68 aact:AA02069|3end_alkylphosphonate_uptake_protein_1406531..1406681_POSITIVE 277 78 31 132 77 aact:AA02315|3end_conserved_hypothetical_protein_1581752..1581902_POSITIVE 257 49 1 83 32 aact:AA01431|3end_conserved_hypothetical_protein_(possible_hydrolase)_960751..960901_POSITIVE 256 74 34 105 58 aact:AA00513|3end_pseudouridylate_synthase_(pseudouridine_synthase)_355686..355836_POSITIVE 244 45 6 59 20 aact:AA01753|3end_conserved_hypothetical_protein_1185902..1186052_POSITIVE 241 126 86 48 10 aact:AA01140|3end_anaerobic_dimethyl_sulfoxide_reductase,_chain_C_782526..782676_POSITIVE 238 46 4 136 86 aact:AA01913|3end_GMP_synthase_1290171..1290321_POSITIVE 237 31 8 26 5 aact:AA01097|3end_conserved_hypothetical_protein_757685..757835_POSITIVE 235 73 37 136 89 aact:AA01400|3end_phosphopantetheine_adenylyltransferase_946241..946391_POSITIVE 233 50 5 44 12 aact:AA02552|3end_iron_(chelated)_ABC_transporter,_permease_protein_1779420..1779570_POSITIVE 233 56 20 42 7 aact:AA01778|3end_aminopeptidase_A/I;_cytosol_aminopeptidase;_leucyl_aminopeptidase_1199965..1200115_POSITIVE 233 67 21 136 97 aact:AA01650|3end_maltose_transport_inner_membrane_protein_1119849..1119999_POSITIVE 230 65 21 48 11 aact:AA02195|3end_oxaloacetate_decarboxylase_alpha_chain_1496374..1496524_POSITIVE 230 70 22 102 50 aact:AA01739|3end_conserved_hypothetical_protein_1176512..1176662_POSITIVE 229 67 21 142 97 aact:AA02539|3end_transcriptional_activator_1766929..1767079_POSITIVE 228 62 22 92 48 aact:AA02037|3end_hypothetical_protein_1387052..1387202_POSITIVE 228 50 8 137 97 aact:AA01997|3end_probable_translation_factor_1356864..1357014_POSITIVE 227 66 23 62 31 aact:AA02947|3end_riboflavin_biosynthesis_protein_2061874..2062024_POSITIVE