Origin IGS: ttgacacctttctaaaaacggagaggataaatcgtttccaacgtatccctgaataatacgaactgcgtattattggcattaccaccctattttgagaccaaaacgtaggttaagtaagctcggagttatatgcgttaaattaagtttttcgtattgtaaaagattaaattcggcaattgcaatcaattaatttgatctagattataaaataacaaaattctttaaaataaattatataataaattacataacgataaaaaataacaataaaaggtatttatctacctcataataattataaacttacgatttaaaatcatcgttactattgccgaaaacaaaataaaggaaaataaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttattttcctttattttgttttcggcaatagtaacgatgattttaaatcgtaagtttataattattatgaggtagataaataccttttattgttattttttatcgttatgtaatttattatataatttattttaaagaattttgttattttataatctagatcaaattaattgattgcaattgccgaatttaatcttttacaatacgaaaaacttaatttaacgcatataactccgagcttacttaacctacgttttggtctcaaaatagggtggtaatgccaataatacgcagttcgtattattcagggatacgttggaaacgatttatcctctccgtttttagaaaggtgtcaa Mask Tandem Repeat Region ================================================ ttgacacctttctaaaaacggagaggataaatcgtttccaacgtatccctgaataatacgaactgcgtattattggcattaccaccctattttgagaccaaaacgtaggttaagtaagctcggagttatatgcgttaaattaagtttttcgtattgtaaaagattaaattcggcaattgcaatcaattaatttgatctagattataaaataacaaaattctttaaaataaattatataataaattacataacgataaaaaataacaataaaaggtatttatctacctcataataattataaacttacgatttaaaatcatcgttactattgccgaaaacaaaataaaggaaaataaa Find is-nt database================================================ Query_seq: AA02242:AA02243|AA02242:AA02243:molybdenum cofactor biosynthesis protein A:conserved hypothetical protein:->->:1525149..1525505 357 ttgacacctttctaaaaacggagaggataaatcgtttccaacgtatccctgaataatacgaactgcgtattattggcattaccaccctattttgagaccaaaacgtaggttaagtaagctcggagttatatgcgttaaattaagtttttcgtattgtaaaagattaaattcggcaattgcaatcaattaatttgatctagattataaaataacaaaattctttaaaataaattatataataaattacataacgataaaaaataacaataaaaggtatttatctacctcataataattataaacttacgatttaaaatcatcgttactattgccgaaaacaaaataaaggaaaataaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02242:AA02243|AA02242:AA02243:molybdenum cofactor biosynthesis protein A:conserved hypothetical protein:->->:1525149..1525505 357 ttgacacctttctaaaaacggagaggataaatcgtttccaacgtatccctgaataatacgaactgcgtattattggcattaccaccctattttgagaccaaaacgtaggttaagtaagctcggagttatatgcgttaaattaagtttttcgtattgtaaaagattaaattcggcaattgcaatcaattaatttgatctagattataaaataacaaaattctttaaaataaattatataataaattacataacgataaaaaataacaataaaaggtatttatctacctcataataattataaacttacgatttaaaatcatcgttactattgccgaaaacaaaataaaggaaaataaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02242:AA02243|AA02242:AA02243:molybdenum cofactor biosynthesis protein A:conserved hypothetical protein:->->:1525149..1525505 357 ttgacacctttctaaaaacggagaggataaatcgtttccaacgtatccctgaataatacgaactgcgtattattggcattaccaccctattttgagaccaaaacgtaggttaagtaagctcggagttatatgcgttaaattaagtttttcgtattgtaaaagattaaattcggcaattgcaatcaattaatttgatctagattataaaataacaaaattctttaaaataaattatataataaattacataacgataaaaaataacaataaaaggtatttatctacctcataataattataaacttacgatttaaaatcatcgttactattgccgaaaacaaaataaaggaaaataaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttgacacctttctaaaaacggagaggataaatcgtttccaacgtatccctgaataatacgaactgcgtattattggcattaccaccctattttgagaccaaaacgtaggttaagtaagctcggagttatatgcgttaaattaagtttttcgtattgtaaaagattaaattcggcaattgcaatcaattaatttgatctagattataaaataacaaaattctttaaaataaattatataataaattacataacgataaaaaataacaataaaaggtatttatctacctcataataattataaacttacgatttaaaatcatcgttactattgccgaaaacaaaataaaggaaaataaa Predict ORF larger than 30AA ================================================ Protein_Len: 45 Strand: + Start: 53 End: 187 .................................................... M I R T A Y Y W H Y H P I L R P K R R L S K L G V I C V K L S F S Y C K R L N S A I A I N .......................................................................................................................................................................... Protein_Len: 35 Strand: - Start: 82 End: 186 ................................................................................. W G I K L G F R L N L L S P T I H T L N L K E Y Q L L N F E A I A I M ........................................................................................................................................................................... ttgacacctttctaaaaacggagaggataaatcgtttccaacgtatccctgaataatacgaactgcgtattattggcattaccaccctattttgagaccaaaacgtaggttaagtaagctcggagttatatgcgttaaattaagtttttcgtattgtaaaagattaaattcggcaattgcaatcaattaatttgatctagattataaaataacaaaattctttaaaataaattatataataaattacataacgataaaaaataacaataaaaggtatttatctacctcataataattataaacttacgatttaaaatcatcgttactattgccgaaaacaaaataaaggaaaataaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 231 End: 260 ......................................................................................................................................................................................................................................TTTTTATCGTTATGTAATTTATTATATAAT................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttgacacctttctaaaaacggagaggataaatcgtttccaacgtatccctgaataatacgaactgcgtattattggcattaccaccctattttgagaccaaaacgtaggttaagtaagctcggagttatatgcgttaaattaagtttttcgtattgtaaaagattaaattcggcaattgcaatcaattaatttgatctagattataaaataacaaaattctttaaaataaattatataataaattacataacgataaaaaataacaataaaaggtatttatctacctcataataattataaacttacgatttaaaatcatcgttactattgccgaaaacaaaataaaggaaaataaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02242:AA02243|AA02242:AA02243:molybdenum cofactor biosynthesis protein A:conserved hypothetical protein:->->:1525149..1525505 357 ttgacacctttctaaaaacggagaggataaatcgtttccaacgtatccctgaataatacgaactgcgtattattggcattaccaccctattttgagaccaaaacgtaggttaagtaagctcggagttatatgcgttaaattaagtttttcgtattgtaaaagattaaattcggcaattgcaatcaattaatttgatctagattataaaataacaaaattctttaaaataaattatataataaattacataacgataaaaaataacaataaaaggtatttatctacctcataataattataaacttacgatttaaaatcatcgttactattgccgaaaacaaaataaaggaaaataaa Intra-Species Hit: Count: 1 Min: 1 Max: 357 Len: 357 Subject: aact_AA02242_AA02243|molybdenum cofactor biosynthesis protein A:conserved hypothetical protein|POSITIVE:POSITIVE|[1525149,1525505]|357 HSP 1 e-value: 2.0E-97 bit: 357.0 Len: 180 Query Start:1 Query End:180 Subject Strand: POSITIVE Subject Start: 1 Subject End: 180 ttgacacctttctaaaaacggagaggataaatcgtttccaacgtatccctgaataatacgaactgcgtattattggcattaccaccctattttgagaccaaaacgtaggttaagtaagctcggagttatatgcgttaaattaagtttttcgtattgtaaaagattaaattcggcaattgc................................................................................................................................................................................. ttgacacctttctaaaaacggagaggataaatcgtttccaacgtatccctgaataatacgaactgcgtattattggcattaccaccctattttgagaccaaaacgtaggttaagtaagctcggagttatatgcgttaaattaagtttttcgtattgtaaaagattaaattcggcaattgc................................................................................................................................................................................. HSP 2 e-value: 8.0E-41 bit: 168.0 Len: 85 Query Start:273 Query End:357 Subject Strand: POSITIVE Subject Start: 273 Subject End: 357 ................................................................................................................................................................................................................................................................................ggtatttatctacctcataataattataaacttacgatttaaaatcatcgttactattgccgaaaacaaaataaaggaaaataaa ................................................................................................................................................................................................................................................................................ggtatttatctacctcataataattataaacttacgatttaaaatcatcgttactattgccgaaaacaaaataaaggaaaataaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUGACACCUUUCUAAAAACGGAGAGGAUAAAUCGUUUCCAACGUAUCCCUGAAUAAUACGAACUGCGUAUUAUUGGCAUUACCACCCUAUUUUGAGACCAAAACGUAGGUUAAGUAAGCUCGGAGUUAUAUGCGUUAAAUUAAGUUUUUCGUAUUGUAAAAGAUUAAAUUCGGCAAUUGCAAUCAAUUAAUUUGAUCUAGAUUAUAAAAUAACAAAAUUCUUUAAAAUAAAUUAUAUAAUAAAUUACAUAACGAUAAAAAAUAACAAUAAAAGGUAUUUAUCUACCUCAUAAUAAUUAUAAACUUACGAUUUAAAAUCAUCGUUACUAUUGCCGAAAACAAAAUAAAGGAAAAUAAA ......(((((((......)))))))........(((((......(((...(((((((((.....))))))))).((.((((...(((((((((....))))..)))))....))))))..))).(((((((((..((((...))))..)))).))))).........(((((((((....(((((.....)))))....................................................((((((.................(((((......)))))....................(((.....))).))))))..)))))))))...........)))))..... (-60.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUAUUUUCCUUUAUUUUGUUUUCGGCAAUAGUAACGAUGAUUUUAAAUCGUAAGUUUAUAAUUAUUAUGAGGUAGAUAAAUACCUUUUAUUGUUAUUUUUUAUCGUUAUGUAAUUUAUUAUAUAAUUUAUUUUAAAGAAUUUUGUUAUUUUAUAAUCUAGAUCAAAUUAAUUGAUUGCAAUUGCCGAAUUUAAUCUUUUACAAUACGAAAAACUUAAUUUAACGCAUAUAACUCCGAGCUUACUUAACCUACGUUUUGGUCUCAAAAUAGGGUGGUAAUGCCAAUAAUACGCAGUUCGUAUUAUUCAGGGAUACGUUGGAAACGAUUUAUCCUCUCCGUUUUUAGAAAGGUGUCAA ........(((((...(((..(((((((((.((((((((((........(....)...(((((...((.((((((......)))))).))..)))))...))))))))))((((((.((((....(((((..(((.(((((......))))).)))..))))).....)))).)))))).)))))))))..)))..............((((((........................(((((((...(((..((((((....)))))))))..))))).)).(((((((((.....)))))))))..((((((.((((....)))).))))))....))))))..)))))...... (-75.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 311 89 41 155 100 aact:AA01355|5end_large_subunit_pseudouridylate_synthase_D_917228..917458_POSITIVE 294 119 75 54 11 aact:AA01296|5end_shikimate_kinase_(shikimic_acid_kinase_I)_878384..878614_POSITIVE 285 138 92 57 14 aact:AA01345|5end_conserved_hypothetical_protein_(possible_membrane_protein)_912624..912854_POSITIVE 277 70 21 161 118 aact:AA01674|5end_hypothetical_protein_1132017..1132247_POSITIVE 276 87 42 95 42 aact:AA00141|5end_hypoxanthine_phosphoribosyl_transferase_(HPRT)_100814..101044_POSITIVE 267 122 81 168 124 aact:AA00983|5end_aldo-keto_reductase_687570..687800_POSITIVE 265 87 42 194 149 aact:AA02375|5end_D-3-phosphoglycerate_dehydrogenase_1626514..1626744_POSITIVE 264 110 62 108 66 aact:AA02254|5end_2-oxoglutarate/malate_translocator_1534846..1535076_POSITIVE 263 67 21 56 4 aact:AA02142|5end_outer_membrane_protein_OprM_precursor_1466037..1466267_POSITIVE 261 110 63 201 149 aact:AA01338|5end_hypothetical_protein_904967..905197_POSITIVE 255 110 63 204 158 aact:AA01434|5end_conserved_hypothetical_protein_(possible_o-antigen_ligase)_961631..961861_POSITIVE 253 95 58 176 137 aact:AA01417|5end_probable_4-diphosphocytidyl-2C-methyl-D-erthyritol_synthase_(MEP_cytidyltransferase)_(MCT)_953256..953486_POSITIVE 253 125 83 190 133 aact:AA00786|5end_possible_protein-export_membrane_protein_539975..540205_POSITIVE 251 110 61 200 139 aact:AA02689|5end_hydrogenase-4_component_F_1874620..1874850_POSITIVE 248 85 42 199 147 aact:AA01255|5end_hypothetical_protein_852656..852886_POSITIVE 246 86 41 230 178 aact:AA03012|5end_conserved_hypothetical_protein_2104216..2104446_POSITIVE 241 53 12 148 106 aact:AA02788|5end_possible_ABC-related_substrate_binding_protein_1955402..1955632_POSITIVE 240 148 104 57 12 aact:AA01841|5end_conserved_hypothetical_protein_1237514..1237744_POSITIVE 239 60 14 148 94 aact:AA01187|5end_conserved_hypothetical_protein_813331..813561_POSITIVE 239 52 19 98 50 aact:AA00314|5end_conserved_hypothetical_protein_(possible_Kmt1_protein)_212999..213229_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 276 87 42 73 20 aact:AA00140|3end_hypothetical_protein_100872..101022_POSITIVE 271 129 89 149 103 aact:AA01498|3end_conserved_hypothetical_protein_1008252..1008402_POSITIVE 267 60 20 46 11 aact:AA02020|3end_conserved_hypothetical_protein_(possible_tetrapyrrole_methylase_family_protein)_1374742..1374892_POSITIVE 267 122 81 101 57 aact:AA00982|3end_aldo-keto_reductase_687583..687733_POSITIVE 253 88 41 69 13 aact:AA02002|3end_hypothetical_protein_1360407..1360557_POSITIVE 248 79 38 116 80 aact:AA02695|3end_hydrogenase-3_component_G_1879418..1879568_POSITIVE 248 99 63 70 28 aact:AA01567|3end_conserved_hypothetical_protein_(possible_cell-wall-associated_hydrolase)_1055215..1055365_POSITIVE 246 99 54 120 76 aact:AA01247|3end_hypothetical_protein_849944..850094_POSITIVE 241 53 12 61 19 aact:AA02787|3end_ribose_ABC_transporter,_sugar_binding_protein_1955395..1955545_POSITIVE 240 337 294 151 95 aact:AA01022|3end_tRNA_(5-methylaminomethyl-2-thiouridylate)_methyltransferase_715917..716067_POSITIVE 239 60 14 138 84 aact:AA01185|3end_conserved_hypothetical_protein_(possible_membrane_protein)_813401..813551_POSITIVE 239 52 19 76 28 aact:AA00313|3end_hypothetical_protein_213057..213207_POSITIVE 236 64 38 88 54 aact:AA01774|3end_5,10-methylenetetrahydrofolate_reductase_1195976..1196126_POSITIVE 236 66 22 106 60 aact:AA00317|3end_DNA_mismatch_repair_protein_218110..218260_POSITIVE 233 80 32 99 52 aact:AA02969|3end_beta_tyrosinase_(tyrosine_phenol-lyase)_2076417..2076567_POSITIVE 230 127 82 63 18 aact:AA01043|3end_conserved_hypothetical_protein_726810..726960_POSITIVE 228 130 83 57 5 aact:AA01012|3end_probable_carbon_storage_regulator_homolog_708074..708224_POSITIVE 228 119 74 59 9 aact:AA00181|3end_hypothetical_protein_128855..129005_POSITIVE 226 126 83 49 11 aact:AA01913|3end_GMP_synthase_1290171..1290321_POSITIVE 225 58 14 93 37 aact:AA00596|3end_uridylate_kinase_408157..408307_POSITIVE