Origin IGS: ctattcctccgacgactcaaaataattttaaaatattcaattggtatcacccgataccaagaaaaacggtcgcttattctagcataaagcgaccgttcttttccaagaaaaccacgagattttacgctcagcccgaaaaaaatccctgtttcatgaagaaatcaaggatttttccgttagggataaaataggttcagcctta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaggctgaacctattttatccctaacggaaaaatccttgatttcttcatgaaacagggatttttttcgggctgagcgtaaaatctcgtggttttcttggaaaagaacggtcgctttatgctagaataagcgaccgtttttcttggtatcgggtgataccaattgaatattttaaaattattttgagtcgtcggaggaatag Mask Tandem Repeat Region ================================================ ctattcctccgacgactcaaaataattttaaaatattcaattggtatcacccgataccaagaaaaacggtcgcttattctagcataaagcgaccgttcttttccaagaaaaccacgagattttacgctcagcccgaaaaaaatccctgtttcatgaagaaatcaaggatttttccgttagggataaaataggttcagcctta Find is-nt database================================================ Query_seq: AA02236:AA02237|AA02236:AA02237:stringent starvation protein A:molybdopterin biosynthesis protein E chain:->->:1522698..1522899 202 ctattcctccgacgactcaaaataattttaaaatattcaattggtatcacccgataccaagaaaaacggtcgcttattctagcataaagcgaccgttcttttccaagaaaaccacgagattttacgctcagcccgaaaaaaatccctgtttcatgaagaaatcaaggatttttccgttagggataaaataggttcagcctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02236:AA02237|AA02236:AA02237:stringent starvation protein A:molybdopterin biosynthesis protein E chain:->->:1522698..1522899 202 ctattcctccgacgactcaaaataattttaaaatattcaattggtatcacccgataccaagaaaaacggtcgcttattctagcataaagcgaccgttcttttccaagaaaaccacgagattttacgctcagcccgaaaaaaatccctgtttcatgaagaaatcaaggatttttccgttagggataaaataggttcagcctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02236:AA02237|AA02236:AA02237:stringent starvation protein A:molybdopterin biosynthesis protein E chain:->->:1522698..1522899 202 ctattcctccgacgactcaaaataattttaaaatattcaattggtatcacccgataccaagaaaaacggtcgcttattctagcataaagcgaccgttcttttccaagaaaaccacgagattttacgctcagcccgaaaaaaatccctgtttcatgaagaaatcaaggatttttccgttagggataaaataggttcagcctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ctattcctccgacgactcaaaataattttaaaatattcaattggtatcacccgataccaagaaaaacggtcgcttattctagcataaagcgaccgttcttttccaagaaaaccacgagattttacgctcagcccgaaaaaaatccctgtttcatgaagaaatcaaggatttttccgttagggataaaataggttcagcctta Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 3 End: 188 .. M P P T T Q N N F K I F N W Y H P I P R K T V A Y S S I K R P F F S K K T T R F Y A Q P E K N P C F M K K S R I F P L G .............. Protein_Len: 47 Strand: - Start: 3 End: 143 .. N R R R S L I I K F Y E I P I V R Y W S F R D S I R A Y L S R E K G L F G R S K V S L G F F M ........................................................... ctattcctccgacgactcaaaataattttaaaatattcaattggtatcacccgataccaagaaaaacggtcgcttattctagcataaagcgaccgttcttttccaagaaaaccacgagattttacgctcagcccgaaaaaaatccctgtttcatgaagaaatcaaggatttttccgttagggataaaataggttcagcctta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctattcctccgacgactcaaaataattttaaaatattcaattggtatcacccgataccaagaaaaacggtcgcttattctagcataaagcgaccgttcttttccaagaaaaccacgagattttacgctcagcccgaaaaaaatccctgtttcatgaagaaatcaaggatttttccgttagggataaaataggttcagcctta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 75 HP_score: -9.4 Tail_Score: -3.28785 Start: 41 End: 60 Full_Region: aagcgaccgtttttc ttggtatc gggt gataccaa ttgaatattttaaaa ........................................ttggtatcgggtgataccaa.............................................................................................................................................. TransTerm Strand: + Conf: 79 HP_score: -8.3 Tail_Score: -4.17048 Start: 67 End: 95 Full_Region: CGATACCAAGAAAAA CGGTCGCTTAT TCTAGC ATAAAGCGACCG TTCTTTTCCAAGAAA ..................................................................CGGTCGCTTATTCTAGCATAAAGCGACCG........................................................................................................... TransTerm Strand: - Conf: 91 HP_score: -8.3 Tail_Score: -5.16841 Start: 67 End: 95 Full_Region: tttcttggaaaagaa cggtcgctttat gctaga ataagcgaccg tttttcttggtatcg ..................................................................cggtcgctttatgctagaataagcgaccg........................................................................................................... TransTerm Strand: - Conf: 78 HP_score: -3.4 Tail_Score: -5.42259 Start: 143 End: 168 Full_Region: tccctaacggaaaaa tccttgatttc ttcat gaaacaggga tttttttcgggctga ..............................................................................................................................................tccttgatttcttcatgaaacaggga.................................. Find igs database================================================ Query_seq: AA02236:AA02237|AA02236:AA02237:stringent starvation protein A:molybdopterin biosynthesis protein E chain:->->:1522698..1522899 202 ctattcctccgacgactcaaaataattttaaaatattcaattggtatcacccgataccaagaaaaacggtcgcttattctagcataaagcgaccgttcttttccaagaaaaccacgagattttacgctcagcccgaaaaaaatccctgtttcatgaagaaatcaaggatttttccgttagggataaaataggttcagcctta Intra-Species Hit: Count: 1 Min: 1 Max: 202 Len: 202 Subject: aact_AA02236_AA02237|stringent starvation protein A:molybdopterin biosynthesis protein E chain|POSITIVE:POSITIVE|[1522698,1522899]|202 HSP 1 e-value: 2.0E-98 bit: 359.0 Len: 202 Query Start:1 Query End:202 Subject Strand: POSITIVE Subject Start: 1 Subject End: 202 ctattcctccgacgactcaaaataattttaaaatattcaattggtatcacccgataccaagaaaaacggtcgcttattctagcataaagcgaccgttcttttccaagaaaaccacgagattttacgctcagcccgnnnnnnntccctgtttcatgaagaaatcaaggatttttccgttagggataaaataggttcagcctta ctattcctccgacgactcaaaataattttaaaatattcaattggtatcacccgataccaagaaaaacggtcgcttattctagcataaagcgaccgttcttttccaagaaaaccacgagattttacgctcagcccgaaaaaaatccctgtttcatgaagaaatcaaggatttttccgttagggataaaataggttcagcctta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUAUUCCUCCGACGACUCAAAAUAAUUUUAAAAUAUUCAAUUGGUAUCACCCGAUACCAAGAAAAACGGUCGCUUAUUCUAGCAUAAAGCGACCGUUCUUUUCCAAGAAAACCACGAGAUUUUACGCUCAGCCCGAAAAAAAUCCCUGUUUCAUGAAGAAAUCAAGGAUUUUUCCGUUAGGGAUAAAAUAGGUUCAGCCUUA .(((((((..((((..((.................(((..((((((((....))))))))(((((((((((((((...........))))))))))..)))))...)))......(((........))).....)).((((((((.((((((.....)))).)).)))))))).)))))))))))....(((.....))).. (-51.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGGCUGAACCUAUUUUAUCCCUAACGGAAAAAUCCUUGAUUUCUUCAUGAAACAGGGAUUUUUUUCGGGCUGAGCGUAAAAUCUCGUGGUUUUCUUGGAAAAGAACGGUCGCUUUAUGCUAGAAUAAGCGACCGUUUUUCUUGGUAUCGGGUGAUACCAAUUGAAUAUUUUAAAAUUAUUUUGAGUCGUCGGAGGAAUAG ....(((.(.(((........((....)).((((((((((.((((.....))))))))))))))....))).).)))......((((.(((....((..(((((((((((((((((.((......)))))))))))))))).((((((((....))))))))..................)))..))...)))))))..... (-58.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 331 156 115 42 1 aact:AA01424|5end_threonine_deaminase_(dehydratase)_957740..957970_POSITIVE 322 181 141 176 132 aact:AA02430|5end_conserved_hypothetical_protein_1675251..1675481_POSITIVE 308 95 53 219 174 aact:AA01409|5end_lipoprotein_B_950397..950627_POSITIVE 306 96 52 102 58 aact:AA01128|5end_hypothetical_protein_774030..774260_POSITIVE 297 129 86 95 46 aact:AA02091|5end_hypothetical_protein_1424441..1424671_POSITIVE 293 117 71 191 155 aact:AA01068|5end_hypothetical_protein_739669..739899_POSITIVE 291 116 71 92 32 aact:AA01224|5end_CTP_synthase_836221..836451_POSITIVE 288 134 92 208 172 aact:AA02910|5end_ribonuclease_E_2036137..2036367_POSITIVE 287 92 51 60 27 aact:AA01299|5end_competence_protein_C_880946..881176_POSITIVE 285 84 50 224 189 aact:AA00208|5end_D-ribose_ABC_transporter,_permease_protein_/_Galactoside_transport_system_permease_protein_144097..144327_POSITIVE 284 75 33 120 84 aact:AA00936|5end_ribose_ABC_transporter,_permease_protein,_C-terminal_646289..646519_POSITIVE 281 119 71 157 111 aact:AA01473|5end_type_I_restriction-modification_system_S_(specificity_subunit)_989025..989255_POSITIVE 281 198 153 102 59 aact:AA00271|5end_hypothetical_protein_190264..190494_POSITIVE 275 160 111 219 160 aact:AA02006|5end_PEP--fructosephosphotransferase_system_repressor,_DeoR_family_1363930..1364160_POSITIVE 275 97 57 206 164 aact:AA01510|5end_ABC_transporter,_ATP-binding_protein/permease_1016430..1016660_POSITIVE 274 100 51 176 132 aact:AA01070|5end_DNA_repair_protein_740920..741150_POSITIVE 272 127 81 76 25 aact:AA02397|5end_RNA_polymerase_sigma_factor_sigma-70_1647095..1647325_POSITIVE 264 99 51 159 112 aact:AA00454|5end_conserved_hypothetical_protein_318020..318250_POSITIVE 263 95 58 168 129 aact:AA02553|5end_acylphosphatase_1779321..1779551_POSITIVE 263 105 74 225 196 aact:AA01937|5end_sugar_fermentation_stimulation_protein_1308478..1308708_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 297 129 86 123 74 aact:AA02090|3end_glucose-1-phosphate_adenylyltransferase_1424549..1424699_POSITIVE 293 117 71 139 103 aact:AA01067|3end_30S_ribosomal_protein_S2_739697..739847_POSITIVE 287 181 144 42 1 aact:AA02429|3end_methionine_aminopeptidase_1675197..1675347_POSITIVE 287 92 51 57 24 aact:AA01298|3end_competence_protein_D_881023..881173_POSITIVE 284 75 33 79 43 aact:AA00935|3end_ribose_ABC_transporter,_permease_protein,_N-terminal_646328..646478_POSITIVE 281 119 71 54 8 aact:AA01471|3end_type_I_restriction_enzyme_M_(modification_chain)_989002..989152_POSITIVE 281 198 153 119 76 aact:AA00270|3end_malonyl_coA-acyl_carrier_protein_transacylase_190361..190511_POSITIVE 264 99 51 68 21 aact:AA00453|3end_phosphoglyceromutase_318009..318159_POSITIVE 251 117 81 54 9 aact:AA01304|3end_penicillin-binding_protein_1A_885580..885730_POSITIVE 248 107 63 112 64 aact:AA00578|3end_DNA_topoisomerase_395201..395351_POSITIVE 245 119 71 61 20 aact:AA01090|3end_leucyl-tRNA_synthetase,_leucyl-tRNA_ligase_756213..756363_POSITIVE 245 133 91 117 77 aact:AA00093|3end_L-serine_dehydratase_(L-serine_deaminase)_71173..71323_POSITIVE 244 179 143 115 82 aact:AA02418|3end_peptidyl-tRNA_hydrolase_1661811..1661961_POSITIVE 244 160 112 98 44 aact:AA01705|3end_beta-galactosidase_1157980..1158130_POSITIVE 243 110 61 68 5 aact:AA00109|3end_conserved_hypothetical_protein_79153..79303_POSITIVE 242 102 64 145 109 aact:AA01840|3end_conserved_hypothetical_protein_1237554..1237704_POSITIVE 241 99 51 52 8 aact:AA01156|3end_chaperonin_heat_shock_protein_33_homolog_792408..792558_POSITIVE 236 130 85 127 73 aact:AA00384|3end_conserved_hypothetical_protein_(possible_nitrogen_fixation_protein)_261168..261318_POSITIVE 235 148 114 103 59 aact:AA02545|3end_conserved_hypothetical_protein_1774221..1774371_POSITIVE 235 90 41 151 95 aact:AA00015|3end_malate_dehydrogenase_8333..8483_POSITIVE