Origin IGS: taaataattccgtattttttataataatttatttagtgaaattatttataccagttcattgcttttattcaagaaatttaagtcaaatctgtgttccctgtcacaaaaataaaaaattccgatagattttggtaatttttccaacttgaggtaaactaacccggttttcaaaaatgtattaattttgaggtatat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atatacctcaaaattaatacatttttgaaaaccgggttagtttacctcaagttggaaaaattaccaaaatctatcggaattttttatttttgtgacagggaacacagatttgacttaaatttcttgaataaaagcaatgaactggtataaataatttcactaaataaattattataaaaaatacggaattattta Mask Tandem Repeat Region ================================================ taaataattccgtattttttataataatttatttagtgaaattatttataccagttcattgcttttattcaagaaatttaagtcaaatctgtgttccctgtcacaaaaataaaaaattccgatagattttggtaatttttccaacttgaggtaaactaacccggttttcaaaaatgtattaattttgaggtatat Find is-nt database================================================ Query_seq: AA02232:AA02233|AA02232:AA02233:NagC-like transcriptional regulator:asparaginyl-tRNA synthetase:->->:1519822..1520016 195 taaataattccgtattttttataataatttatttagtgaaattatttataccagttcattgcttttattcaagaaatttaagtcaaatctgtgttccctgtcacaaaaataaaaaattccgatagattttggtaatttttccaacttgaggtaaactaacccggttttcaaaaatgtattaattttgaggtatat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02232:AA02233|AA02232:AA02233:NagC-like transcriptional regulator:asparaginyl-tRNA synthetase:->->:1519822..1520016 195 taaataattccgtattttttataataatttatttagtgaaattatttataccagttcattgcttttattcaagaaatttaagtcaaatctgtgttccctgtcacaaaaataaaaaattccgatagattttggtaatttttccaacttgaggtaaactaacccggttttcaaaaatgtattaattttgaggtatat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02232:AA02233|AA02232:AA02233:NagC-like transcriptional regulator:asparaginyl-tRNA synthetase:->->:1519822..1520016 195 taaataattccgtattttttataataatttatttagtgaaattatttataccagttcattgcttttattcaagaaatttaagtcaaatctgtgttccctgtcacaaaaataaaaaattccgatagattttggtaatttttccaacttgaggtaaactaacccggttttcaaaaatgtattaattttgaggtatat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaataattccgtattttttataataatttatttagtgaaattatttataccagttcattgcttttattcaagaaatttaagtcaaatctgtgttccctgtcacaaaaataaaaaattccgatagattttggtaatttttccaacttgaggtaaactaacccggttttcaaaaatgtattaattttgaggtatat Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 14 End: 109 ............. M F Y N N L F S E I I Y T S S L L L F K K F K S N L C S L S Q K ...................................................................................... Protein_Len: 34 Strand: + Start: 21 End: 122 .................... M I I Y L V K L F I P V H C F Y S R N L S Q I C V P C H K N K K F R ......................................................................... Protein_Len: 49 Strand: - Start: 49 End: 195 ................................................ V L E N S K N L F N L D F R H E R D C F Y F I G I S K P L K E L K L Y V L G T K L F T N I K L Y M taaataattccgtattttttataataatttatttagtgaaattatttataccagttcattgcttttattcaagaaatttaagtcaaatctgtgttccctgtcacaaaaataaaaaattccgatagattttggtaatttttccaacttgaggtaaactaacccggttttcaaaaatgtattaattttgaggtatat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 82 Start: 128 End: 157 ...............................................................................................................................TTTGGTAATTTTTCCAACTTGAGGTAAACT...................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaataattccgtattttttataataatttatttagtgaaattatttataccagttcattgcttttattcaagaaatttaagtcaaatctgtgttccctgtcacaaaaataaaaaattccgatagattttggtaatttttccaacttgaggtaaactaacccggttttcaaaaatgtattaattttgaggtatat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02232:AA02233|AA02232:AA02233:NagC-like transcriptional regulator:asparaginyl-tRNA synthetase:->->:1519822..1520016 195 taaataattccgtattttttataataatttatttagtgaaattatttataccagttcattgcttttattcaagaaatttaagtcaaatctgtgttccctgtcacaaaaataaaaaattccgatagattttggtaatttttccaacttgaggtaaactaacccggttttcaaaaatgtattaattttgaggtatat Intra-Species Hit: Count: 1 Min: 1 Max: 195 Len: 195 Subject: aact_AA02232_AA02233|NagC-like transcriptional regulator:asparaginyl-tRNA synthetase|POSITIVE:POSITIVE|[1519822,1520016]|195 HSP 1 e-value: 3.0E-85 bit: 315.0 Len: 195 Query Start:1 Query End:195 Subject Strand: POSITIVE Subject Start: 1 Subject End: 195 taaataattccgtattttttataataatttatttagtgaaattatttataccagttcattgcttttattcaagaaatttaagtcaaatctgtgttccctgtcacnnnnnnnnnnnnttccgatagattttggtaatttttccaacttgaggtaaactaacccggttttcaaaaatgtattaattttgaggtatat taaataattccgtattttttataataatttatttagtgaaattatttataccagttcattgcttttattcaagaaatttaagtcaaatctgtgttccctgtcacaaaaataaaaaattccgatagattttggtaatttttccaacttgaggtaaactaacccggttttcaaaaatgtattaattttgaggtatat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUAAUUCCGUAUUUUUUAUAAUAAUUUAUUUAGUGAAAUUAUUUAUACCAGUUCAUUGCUUUUAUUCAAGAAAUUUAAGUCAAAUCUGUGUUCCCUGUCACAAAAAUAAAAAAUUCCGAUAGAUUUUGGUAAUUUUUCCAACUUGAGGUAAACUAACCCGGUUUUCAAAAAUGUAUUAAUUUUGAGGUAUAU (((((((.....(((.....))).....)))))))...........((((((......(((((.....(((((................((((........))))......(((((((((((......)))).)))))))....)))))))))).............((((((.........)))))))))))). (-24.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAUACCUCAAAAUUAAUACAUUUUUGAAAACCGGGUUAGUUUACCUCAAGUUGGAAAAAUUACCAAAAUCUAUCGGAAUUUUUUAUUUUUGUGACAGGGAACACAGAUUUGACUUAAAUUUCUUGAAUAAAAGCAAUGAACUGGUAUAAAUAAUUUCACUAAAUAAAUUAUUAUAAAAAAUACGGAAUUAUUUA .......((((((.........))))))..(((((.(((.((((..(((..((((........))))........(((((((.(((..((((((........))))))..)))...))))))).))).)))).....))).))))).(((((((((((..........................))))))))))) (-27.07) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 278 100 65 89 52 aact:AA00825|5end_P-protein_(chorismate_mutase/prephenate_dehydratase)_567020..567250_POSITIVE 239 104 62 172 115 aact:AA01584|5end_host_factor-I_protein_1065589..1065819_POSITIVE 228 189 141 71 22 aact:AA02092|5end_glycogen_synthase_1424674..1424904_POSITIVE 219 169 125 182 138 aact:AA00615|5end_cation_transport_protein_420529..420759_POSITIVE 214 122 91 57 24 aact:AA00204|5end_(di)nucleoside_polyphosphate_hydrolase;_possible_invasion_protein_141346..141576_POSITIVE 213 97 56 155 110 aact:AA00132|5end_conserved_hypothetical_protein_(possible_regulator_protein,_VirR_family)_94938..95168_POSITIVE 210 124 88 83 45 aact:AA02515|5end_flavodoxin_1748721..1748951_POSITIVE 207 179 135 214 169 aact:AA01848|5end_argininosuccinate_synthetase_1242278..1242508_POSITIVE 206 151 120 80 45 aact:AA00646|5end_2-dehydro-3-deoxyphosphooctonate_aldolase_441474..441704_POSITIVE 204 97 51 229 174 aact:AA01466|5end_mannose-specific_phosphotransferase_system_IIC_985482..985712_POSITIVE 204 99 51 103 51 aact:AA00348|5end_50S_ribosomal_protein_L1_232570..232800_POSITIVE 203 168 121 162 108 aact:AA02794|5end_myo-inositol_catabolism_protein_1962042..1962272_POSITIVE 203 170 138 168 132 aact:AA00071|5end_conserved_hypothetical_protein_(possible_zinc_protease)_53463..53693_POSITIVE 202 130 81 144 98 aact:AA01496|5end_glucose_inhibited_division_protein_A_1005125..1005355_POSITIVE 201 106 86 31 14 aact:AA02250|5end_possible_cytochrome_c-type_protein_1530125..1530355_POSITIVE 201 153 113 60 18 aact:AA01159|5end_phosphatase_792828..793058_POSITIVE 198 152 113 225 180 aact:AA03003|5end_GTP-binding_membrane_protein_2096544..2096774_POSITIVE 198 152 113 104 59 aact:AA03002|5end_hypothetical_protein_2096423..2096653_POSITIVE 198 137 93 53 9 aact:AA02738|5end_phosphate_permease_1919372..1919602_POSITIVE 197 123 82 70 21 aact:AA01421|5end_phosphoglycolate_phosphatase_955055..955285_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 278 100 65 114 77 aact:AA00824|3end_hypothetical_protein_567125..567275_POSITIVE 225 100 58 151 90 aact:AA01582|3end_hypothetical_protein_1065651..1065801_POSITIVE 219 169 125 139 95 aact:AA00614|3end_ribonuclease_HII_(RNase_HII)_420566..420716_POSITIVE 214 122 91 51 18 aact:AA00202|3end_conserved_hypothetical_protein_(possible_membrane_protein)_141420..141570_POSITIVE 213 97 56 121 76 aact:AA00131|3end_transcriptional_regulator_(MerR_family)_94984..95134_POSITIVE 204 99 51 95 43 aact:AA00347|3end_50S_ribosomal_protein_L11_232642..232792_POSITIVE 202 102 61 78 44 aact:AA01453|3end_D-xylose_isomerase_974853..975003_POSITIVE 201 153 113 44 2 aact:AA01158|3end_ribosome_associated_heat_shock_protein_15_homolog_792892..793042_POSITIVE 200 166 123 132 88 aact:AA01262|3end_hydrolase_860087..860237_POSITIVE 198 152 113 88 43 aact:AA03001|3end_probable_glycyl_radical_protein/pyruvate-formate_lyase_2096487..2096637_POSITIVE 197 123 82 64 15 aact:AA01419|3end_D-ribulose-phosphate-3_epimerase_955129..955279_POSITIVE 194 103 76 106 78 aact:AA00706|3end_tRNA/rRNA_methyltransferase_485592..485742_POSITIVE 194 155 116 88 50 aact:AA00549|3end_ferredoxin-type_protein_376521..376671_POSITIVE 194 99 54 45 2 aact:AA00292|3end_dihydrofolate_reductase_202338..202488_POSITIVE 193 155 112 150 100 aact:AA02626|3end_conserved_hypothetical_protein_1829202..1829352_POSITIVE 191 189 141 74 22 aact:AA02808|3end_conserved_hypothetical_protein_(possible_serine_hydroxymethyltransferase)_1977944..1978094_POSITIVE 190 189 141 51 2 aact:AA02163|3end_integration_host_factor_alpha-subunit_1479863..1480013_POSITIVE 188 167 133 111 71 aact:AA02001|3end_conserved_hypothetical_protein_1360239..1360389_POSITIVE 188 135 91 130 82 aact:AA00275|3end_conserved_hypothetical_protein_193237..193387_POSITIVE 186 189 142 58 14 aact:AA02260|3end_pseudouridylate_synthase_I_1537962..1538112_POSITIVE