Origin IGS: tagaaggcaccagggttgtagcgaacgataaaaaaatcgtaggggggacaactctctacgattttttgttgatataccgtaaaaaacatcggtaaaacccaccgcactttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaagtgcggtgggttttaccgatgttttttacggtatatcaacaaaaaatcgtagagagttgtcccccctacgatttttttatcgttcgctacaaccctggtgccttcta Mask Tandem Repeat Region ================================================ tagaaggcaccagggttgtagcgaacgataaaaaaatcgtaggggggacaactctctacgattttttgttgatataccgtaaaaaacatcggtaaaacccaccgcactttta Find is-nt database================================================ Query_seq: AA02230:AA02231|AA02230:AA02231:sugar kinase:probable dethiobiotin synthetase 1:->->:1517678..1517789 112 tagaaggcaccagggttgtagcgaacgataaaaaaatcgtaggggggacaactctctacgattttttgttgatataccgtaaaaaacatcggtaaaacccaccgcactttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02230:AA02231|AA02230:AA02231:sugar kinase:probable dethiobiotin synthetase 1:->->:1517678..1517789 112 tagaaggcaccagggttgtagcgaacgataaaaaaatcgtaggggggacaactctctacgattttttgttgatataccgtaaaaaacatcggtaaaacccaccgcactttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02230:AA02231|AA02230:AA02231:sugar kinase:probable dethiobiotin synthetase 1:->->:1517678..1517789 112 tagaaggcaccagggttgtagcgaacgataaaaaaatcgtaggggggacaactctctacgattttttgttgatataccgtaaaaaacatcggtaaaacccaccgcactttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagaaggcaccagggttgtagcgaacgataaaaaaatcgtaggggggacaactctctacgattttttgttgatataccgtaaaaaacatcggtaaaacccaccgcactttta Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: - Start: 3 End: 107 .. F A G P N Y R V I F F D Y P P C S E V I K Q Q Y V T F F M P L V W R M ..... Protein_Len: 34 Strand: - Start: 1 End: 102 L L C W P Q L S R Y F F R L P S L E R R N K T S I G Y F V D T F G M .......... tagaaggcaccagggttgtagcgaacgataaaaaaatcgtaggggggacaactctctacgattttttgttgatataccgtaaaaaacatcggtaaaacccaccgcactttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagaaggcaccagggttgtagcgaacgataaaaaaatcgtaggggggacaactctctacgattttttgttgatataccgtaaaaaacatcggtaaaacccaccgcactttta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -8.6 Tail_Score: -5.5353 Start: 37 End: 61 Full_Region: CGAACGATAAAAAAA TCGTAGGGGG GACAA CTCTCTACGA TTTTTTGTTGATATA ....................................TCGTAGGGGGGACAACTCTCTACGA................................................... TransTerm Strand: - Conf: 88 HP_score: -4.2 Tail_Score: -5.71983 Start: 37 End: 61 Full_Region: tatatcaacaaaaaa tcgtagagag ttgtc ccccctacga tttttttatcgttcg ....................................tcgtagagagttgtcccccctacga................................................... Find igs database================================================ Query_seq: AA02230:AA02231|AA02230:AA02231:sugar kinase:probable dethiobiotin synthetase 1:->->:1517678..1517789 112 tagaaggcaccagggttgtagcgaacgataaaaaaatcgtaggggggacaactctctacgattttttgttgatataccgtaaaaaacatcggtaaaacccaccgcactttta Intra-Species Hit: Count: 1 Min: 1 Max: 112 Len: 112 Subject: aact_AA02230_AA02231|sugar kinase:probable dethiobiotin synthetase 1|POSITIVE:POSITIVE|[1517678,1517789]|112 HSP 1 e-value: 6.0E-45 bit: 180.0 Len: 112 Query Start:1 Query End:112 Subject Strand: POSITIVE Subject Start: 1 Subject End: 112 tagaaggcaccagggttgtagcgaacgatnnnnnnntcgtaggggggacaactctctacgattttttgttgatataccgtaaaaaacatcggtaaaacccaccgcactttta tagaaggcaccagggttgtagcgaacgataaaaaaatcgtaggggggacaactctctacgattttttgttgatataccgtaaaaaacatcggtaaaacccaccgcactttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAAGGCACCAGGGUUGUAGCGAACGAUAAAAAAAUCGUAGGGGGGACAACUCUCUACGAUUUUUUGUUGAUAUACCGUAAAAAACAUCGGUAAAACCCACCGCACUUUUA ((((((((....(((((........((((((((((.((((((((((.....))))))))))))))))))))...(((((..........))))).)))))...)).)))))) (-38.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAGUGCGGUGGGUUUUACCGAUGUUUUUUACGGUAUAUCAACAAAAAAUCGUAGAGAGUUGUCCCCCCUACGAUUUUUUUAUCGUUCGCUACAACCCUGGUGCCUUCUA .....(..(.(.(((((.((.(((........((((((.......((((((((((((.(.(......).)))))))))))))))))))))).)).)))))).)..)...... (-26.51) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 385 110 82 109 81 aact:AA01008|5end_mazG_beta-lactamase_regulatory_protein_homolog_703470..703700_POSITIVE 363 110 78 177 143 aact:AA02144|5end_iron_(III)_ABC_transporter,_permease_protein_1467583..1467813_POSITIVE 358 110 76 122 82 aact:AA01078|5end_transcriptional_regulator_(probable_MerR_family)_746873..747103_POSITIVE 355 110 67 130 89 aact:AA01933|5end_hypothetical_protein_1306060..1306290_POSITIVE 355 110 62 193 136 aact:AA00268|5end_acyl_carrier_protein_(ACP)_188104..188334_POSITIVE 354 110 62 150 109 aact:AA00324|5end_tRNA_(Uracil-5-)-methyltransferase_(tRNA(M-5-U54)-methyltransferase)_220147..220377_POSITIVE 351 110 77 112 75 aact:AA00160|5end_pilA-like_biogenesis_protein_/_prepilin_peptidase_dependent_proteinD_112836..113066_POSITIVE 336 45 1 192 151 aact:AA00559|5end_conserved_hypothetical_protein_383167..383397_POSITIVE 335 110 82 121 93 aact:AA01390|5end_LexA_repressor_937059..937289_POSITIVE 334 109 67 74 37 aact:AA00750|5end_periplasmic_D-galactose-binding_ABC_transport_protein_514904..515134_POSITIVE 332 110 82 196 168 aact:AA01471|5end_type_I_restriction_enzyme_M_(modification_chain)_987321..987551_POSITIVE 329 80 39 194 150 aact:AA01450|5end_hypothetical_protein_971860..972090_POSITIVE 326 110 83 27 1 aact:AA02143|5end_hypothetical_protein_1467433..1467663_POSITIVE 324 110 77 38 1 aact:AA01660|5end_hypothetical_protein_1125035..1125265_POSITIVE 318 110 77 42 9 aact:AA00267|5end_hypothetical_protein_187953..188183_POSITIVE 315 110 82 165 137 aact:AA01981|5end_4-alpha-glucanotransferase_1337202..1337432_POSITIVE 314 110 62 149 102 aact:AA00501|5end_hypothetical_protein_347293..347523_POSITIVE 307 110 67 200 156 aact:AA02944|5end_30S_ribosomal_protein_S20_2058688..2058918_POSITIVE 307 110 67 47 3 aact:AA02941|5end_hypothetical_protein_2058535..2058765_POSITIVE 306 50 7 81 35 aact:AA00546|5end_cytochrome_C-type_protein_(denitrification_system_component)_374427..374657_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 394 110 61 81 28 aact:AA02865|3end_iron-regulated_outer_membrane_protein_(omp64)_2012542..2012692_POSITIVE 382 78 31 77 24 aact:AA02230|3end_sugar_kinase_1517617..1517767_POSITIVE 363 110 78 46 12 aact:AA02142|3end_outer_membrane_protein_OprM_precursor_1467532..1467682_POSITIVE 355 110 67 82 41 aact:AA01932|3end_tRNA_pseudouridine_synthase_B_1306092..1306242_POSITIVE 354 110 62 145 104 aact:AA00323|3end_conserved_hypothetical_protein_(possible_SAM-dependent_methyltransferase)_220222..220372_POSITIVE 351 110 77 131 94 aact:AA00158|3end_ampD_signalling_protein_(N-acetylmuramyl-L-alanine_amidase)_112935..113085_POSITIVE 346 109 62 151 95 aact:AA00267|3end_hypothetical_protein_188143..188293_POSITIVE 340 110 79 39 8 aact:AA01077|3end_transposase_746870..747020_POSITIVE 335 110 82 140 112 aact:AA01389|3end_hypothetical_protein_937158..937308_POSITIVE 315 110 82 140 112 aact:AA01978|3end_hypothetical_protein_1337257..1337407_POSITIVE 314 110 62 132 85 aact:AA00500|3end_hypothetical_protein_347356..347506_POSITIVE 314 110 62 55 8 aact:AA00499|3end_pyridoxamine-5'-phosphate_oxidase_347279..347429_POSITIVE 307 110 67 84 40 aact:AA02940|3end_conserved_hypothetical_protein_2058652..2058802_POSITIVE 305 59 12 83 35 aact:AA01675|3end_probable_multidrug_resistance_protein;_multidrug-efflux_transporter_1133589..1133739_POSITIVE 301 48 7 82 47 aact:AA01369|3end_rod_shape-determining_protein_925513..925663_POSITIVE 301 48 3 125 80 aact:AA00450|3end_outer_membrane_antigenic_lipoprotein_b_precursor_315156..315306_POSITIVE 300 106 78 151 121 aact:AA02143|3end_hypothetical_protein_1467641..1467791_POSITIVE 299 110 76 68 29 aact:AA01179|3end_beta-ketoacyl-ACP_reductase;_short-chain_alcohol_dehydrogenase_808801..808951_POSITIVE 298 110 77 146 114 aact:AA01092|3end_hypothetical_protein_756361..756511_POSITIVE 297 49 6 65 9 aact:AA02893|3end_oligopeptide_transport_system_permease_protein,_N-terminal_2027544..2027694_POSITIVE