Origin IGS: taaaaaaacacgagaaaaaaccaccgcactttttacaacaaaacagatgatagcgcgcgttttttaacgcgtgccaaattttacggcacacaccggcaaatcaagccttattccatgaatcacgaggatcgcaca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgtgcgatcctcgtgattcatggaataaggcttgatttgccggtgtgtgccgtaaaatttggcacgcgttaaaaaacgcgcgctatcatctgttttgttgtaaaaagtgcggtggttttttctcgtgttttttta Mask Tandem Repeat Region ================================================ taaaaaaacacgagaaaaaaccaccgcactttttacaacaaaacagatgatagcgcgcgttttttaacgcgtgccaaattttacggcacacaccggcaaatcaagccttattccatgaatcacgaggatcgcaca Find is-nt database================================================ Query_seq: AA02210:AA02212|AA02210:AA02212:ribonucleoside-diphosphate reductase alpha chain:ribonucleoside-diphosphate reductase beta chain:->->:1506017..1506151 135 taaaaaaacacgagaaaaaaccaccgcactttttacaacaaaacagatgatagcgcgcgttttttaacgcgtgccaaattttacggcacacaccggcaaatcaagccttattccatgaatcacgaggatcgcaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02210:AA02212|AA02210:AA02212:ribonucleoside-diphosphate reductase alpha chain:ribonucleoside-diphosphate reductase beta chain:->->:1506017..1506151 135 taaaaaaacacgagaaaaaaccaccgcactttttacaacaaaacagatgatagcgcgcgttttttaacgcgtgccaaattttacggcacacaccggcaaatcaagccttattccatgaatcacgaggatcgcaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02210:AA02212|AA02210:AA02212:ribonucleoside-diphosphate reductase alpha chain:ribonucleoside-diphosphate reductase beta chain:->->:1506017..1506151 135 taaaaaaacacgagaaaaaaccaccgcactttttacaacaaaacagatgatagcgcgcgttttttaacgcgtgccaaattttacggcacacaccggcaaatcaagccttattccatgaatcacgaggatcgcaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaaaaacacgagaaaaaaccaccgcactttttacaacaaaacagatgatagcgcgcgttttttaacgcgtgccaaattttacggcacacaccggcaaatcaagccttattccatgaatcacgaggatcgcaca Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: - Start: 2 End: 91 . F F C S F F G G C K K C C F L H Y R A N K L A H W I K R C M ............................................ taaaaaaacacgagaaaaaaccaccgcactttttacaacaaaacagatgatagcgcgcgttttttaacgcgtgccaaattttacggcacacaccggcaaatcaagccttattccatgaatcacgaggatcgcaca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaaaaacacgagaaaaaaccaccgcactttttacaacaaaacagatgatagcgcgcgttttttaacgcgtgccaaattttacggcacacaccggcaaatcaagccttattccatgaatcacgaggatcgcaca Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 75 HP_score: -10.1 Tail_Score: -2.97662 Start: 53 End: 74 Full_Region: gtgccgtaaaatttg gcacgcgtt aaaa aacgcgcgc tatcatctgttttgt ....................................................gcacgcgttaaaaaacgcgcgc............................................................. Find igs database================================================ Query_seq: AA02210:AA02212|AA02210:AA02212:ribonucleoside-diphosphate reductase alpha chain:ribonucleoside-diphosphate reductase beta chain:->->:1506017..1506151 135 taaaaaaacacgagaaaaaaccaccgcactttttacaacaaaacagatgatagcgcgcgttttttaacgcgtgccaaattttacggcacacaccggcaaatcaagccttattccatgaatcacgaggatcgcaca Intra-Species Hit: Count: 1 Min: 9 Max: 135 Len: 127 Subject: aact_AA02210_AA02212|ribonucleoside-diphosphate reductase alpha chain:ribonucleoside-diphosphate reductase beta chain|POSITIVE:POSITIVE|[1506017,1506151]|135 HSP 1 e-value: 3.0E-66 bit: 252.0 Len: 127 Query Start:9 Query End:135 Subject Strand: POSITIVE Subject Start: 9 Subject End: 135 ........cacgagaaaaaaccaccgcactttttacaacaaaacagatgatagcgcgcgttttttaacgcgtgccaaattttacggcacacaccggcaaatcaagccttattccatgaatcacgaggatcgcaca ........cacgagaaaaaaccaccgcactttttacaacaaaacagatgatagcgcgcgttttttaacgcgtgccaaattttacggcacacaccggcaaatcaagccttattccatgaatcacgaggatcgcaca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CACGAGAAAAAACCACCGCACUUUUUACAACAAAACAGAUGAUAGCGCGCGUUUUUUAACGCGUGCCAAAUUUUACGGCACACACCGGCAAAUCAAGCCUUAUUCCAUGAAUCACGAGGAUCGCACA .................((....................((((.(((((((((....))))))))).........(((......)))....))))..((((((((...))))...))))...))... (-25.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUGCGAUCCUCGUGAUUCAUGGAAUAAGGCUUGAUUUGCCGGUGUGUGCCGUAAAAUUUGGCACGCGUUAAAAAACGCGCGCUAUCAUCUGUUUUGUUGUAAAAAGUGCGGUGGUUUUUUCUCGUG ...((((.(((((((.....((.((((((((..(((((..((((....))))..)))))((((.((((((....)))))).))))......)))))))).)).....))))).)).......)))). (-32.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 609 87 41 175 126 aact:AA01982|5end_maltodextrin_phosphorylase_1339387..1339617_POSITIVE 471 67 38 208 182 aact:AA02573|5end_ABC_transporter,_ATP-binding_protein_1790409..1790639_POSITIVE 408 89 41 231 167 aact:AA02581|5end_conserved_hypothetical_protein_1796966..1797196_POSITIVE 387 67 37 162 122 aact:AA02446|5end_conserved_hypothetical_protein_1686841..1687071_POSITIVE 376 50 2 215 153 aact:AA02030|5end_threonyl-tRNA_synthetase_1382171..1382401_POSITIVE 371 74 38 186 152 aact:AA02506|5end_possible_transmembrane_protein_1739287..1739517_POSITIVE 368 98 51 103 60 aact:AA01169|5end_hypothetical_protein_801985..802215_POSITIVE 362 90 42 113 52 aact:AA01432|5end_lysophospholipase_L2_960715..960945_POSITIVE 358 53 13 207 163 aact:AA01992|5end_protease_IV;_signal_peptide_peptidase;_Endopeptidase_IV_1353029..1353259_POSITIVE 345 101 61 88 48 aact:AA01547|5end_deoxyribose-phosphate_aldolase_1036271..1036501_POSITIVE 344 55 13 220 172 aact:AA01824|5end_iron(III)_ABC_transporter,_ATP-binding_protein_1227705..1227935_POSITIVE 344 55 13 78 30 aact:AA01823|5end_hypothetical_protein_1227563..1227793_POSITIVE 344 80 35 202 153 aact:AA01344|5end_magnesium_and_cobalt_transport_protein_911661..911891_POSITIVE 339 99 51 172 115 aact:AA02954|5end_penicillin_tolerance_protein;_hydroxymethylbutenyl_pyrophosphate_reductase_2065610..2065840_POSITIVE 334 120 73 218 173 aact:AA00682|5end_S-adenosylmethionine-dependent_methyltransferase_466322..466552_POSITIVE 331 57 11 218 164 aact:AA02144|5end_iron_(III)_ABC_transporter,_permease_protein_1467583..1467813_POSITIVE 331 57 11 68 14 aact:AA02143|5end_hypothetical_protein_1467433..1467663_POSITIVE 329 48 1 171 122 aact:AA01008|5end_mazG_beta-lactamase_regulatory_protein_homolog_703470..703700_POSITIVE 327 50 1 173 127 aact:AA00324|5end_tRNA_(Uracil-5-)-methyltransferase_(tRNA(M-5-U54)-methyltransferase)_220147..220377_POSITIVE 319 50 12 193 150 aact:AA01255|5end_hypothetical_protein_852656..852886_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 609 87 41 62 13 aact:AA01981|3end_4-alpha-glucanotransferase_1339354..1339504_POSITIVE 371 74 38 67 33 aact:AA02504|3end_lipid_A_biosynthesis_(kdo)2-(lauroyl)-lipid_IVA_acyltransferase_1739248..1739398_POSITIVE 368 98 51 56 13 aact:AA01168|3end_6-phosphogluconolactonase_802018..802168_POSITIVE 362 90 42 69 8 aact:AA01431|3end_conserved_hypothetical_protein_(possible_hydrolase)_960751..960901_POSITIVE 360 59 13 129 74 aact:AA02697|3end_hydrogenase_3_maturation_protease_1880408..1880558_POSITIVE 358 53 13 101 57 aact:AA01991|3end_conserved_hypothetical_protein_(possible_nitroreductase)_1353003..1353153_POSITIVE 350 89 47 42 1 aact:AA01169|3end_hypothetical_protein_802229..802379_POSITIVE 345 101 61 57 17 aact:AA01546|3end_deoxyribose_operon_repressor_1036320..1036470_POSITIVE 344 55 13 106 58 aact:AA01822|3end_lysostaphin_precursor_1227671..1227821_POSITIVE 343 50 4 100 56 aact:AA01611|3end_anthranilate_synthase_component_II_1089619..1089769_POSITIVE 332 90 41 95 31 aact:AA02545|3end_conserved_hypothetical_protein_1774221..1774371_POSITIVE 331 57 11 87 33 aact:AA02142|3end_outer_membrane_protein_OprM_precursor_1467532..1467682_POSITIVE 329 48 1 67 18 aact:AA01006|3end_conserved_hypothetical_protein_(possible_cytosine_deaminas)_703446..703596_POSITIVE 321 90 47 145 109 aact:AA02952|3end_lipoprotein_signal_peptidase_2065687..2065837_POSITIVE 315 67 39 129 101 aact:AA00738|3end_hypothetical_protein_505256..505406_POSITIVE 314 78 39 54 21 aact:AA00697|3end_hypothetical_protein_477174..477324_POSITIVE 313 90 44 56 13 aact:AA02932|3end_lytic_murein_transglycosylase_A_2053544..2053694_POSITIVE 312 90 42 115 61 aact:AA01769|3end_hypothetical_protein_1193132..1193282_POSITIVE 311 87 46 54 8 aact:AA01772|3end_shikimate_5-dehydrogenase_protein_1194942..1195092_POSITIVE 311 35 2 52 12 aact:AA01019|3end_conserved_hypothetical_protein_712719..712869_POSITIVE