Origin IGS: tagcaataagttaagagaatttgaacgaattgaagggtcgcgtttagaaaattgaattggttaagaaaaatgcacaatcactaaaaattaaaaagtgcggtagcgtttttcaatgcctaaaaacacctagaacacccaccgcacttttttgtatgctaaaacacacttacgcgtgtgcaacaatcaaaacaaaattttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaaattttgttttgattgttgcacacgcgtaagtgtgttttagcatacaaaaaagtgcggtgggtgttctaggtgtttttaggcattgaaaaacgctaccgcactttttaatttttagtgattgtgcatttttcttaaccaattcaattttctaaacgcgacccttcaattcgttcaaattctcttaacttattgcta Mask Tandem Repeat Region ================================================ tagcaataagttaagagaatttgaacgaattgaagggtcgcgtttagaaaattgaattggttaagaaaaatgcacaatcactaaaaattaaaaagtgcggtagcgtttttcaatgcctaaaaacacctagaacacccaccgcacttttttgtatgctaaaacacacttacgcgtgtgcaacaatcaaaacaaaattttta Find is-nt database================================================ Query_seq: AA02199:AA02201|AA02199:AA02201:hypothetical protein:transferrin-binding protein 1 precursor:->->:1497962..1498161 200 tagcaataagttaagagaatttgaacgaattgaagggtcgcgtttagaaaattgaattggttaagaaaaatgcacaatcactaaaaattaaaaagtgcggtagcgtttttcaatgcctaaaaacacctagaacacccaccgcacttttttgtatgctaaaacacacttacgcgtgtgcaacaatcaaaacaaaattttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02199:AA02201|AA02199:AA02201:hypothetical protein:transferrin-binding protein 1 precursor:->->:1497962..1498161 200 tagcaataagttaagagaatttgaacgaattgaagggtcgcgtttagaaaattgaattggttaagaaaaatgcacaatcactaaaaattaaaaagtgcggtagcgtttttcaatgcctaaaaacacctagaacacccaccgcacttttttgtatgctaaaacacacttacgcgtgtgcaacaatcaaaacaaaattttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02199:AA02201|AA02199:AA02201:hypothetical protein:transferrin-binding protein 1 precursor:->->:1497962..1498161 200 tagcaataagttaagagaatttgaacgaattgaagggtcgcgtttagaaaattgaattggttaagaaaaatgcacaatcactaaaaattaaaaagtgcggtagcgtttttcaatgcctaaaaacacctagaacacccaccgcacttttttgtatgctaaaacacacttacgcgtgtgcaacaatcaaaacaaaattttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagcaataagttaagagaatttgaacgaattgaagggtcgcgtttagaaaattgaattggttaagaaaaatgcacaatcactaaaaattaaaaagtgcggtagcgtttttcaatgcctaaaaacacctagaacacccaccgcacttttttgtatgctaaaacacacttacgcgtgtgcaacaatcaaaacaaaattttta Predict ORF larger than 30AA ================================================ Protein_Len: 50 Strand: + Start: 51 End: 200 .................................................. M E L V K K N A Q S L K I K K C G S V F Q C L K T P R T P T A L F C M L K H T Y A C A T I K T K F L tagcaataagttaagagaatttgaacgaattgaagggtcgcgtttagaaaattgaattggttaagaaaaatgcacaatcactaaaaattaaaaagtgcggtagcgtttttcaatgcctaaaaacacctagaacacccaccgcacttttttgtatgctaaaacacacttacgcgtgtgcaacaatcaaaacaaaattttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagcaataagttaagagaatttgaacgaattgaagggtcgcgtttagaaaattgaattggttaagaaaaatgcacaatcactaaaaattaaaaagtgcggtagcgtttttcaatgcctaaaaacacctagaacacccaccgcacttttttgtatgctaaaacacacttacgcgtgtgcaacaatcaaaacaaaattttta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02199:AA02201|AA02199:AA02201:hypothetical protein:transferrin-binding protein 1 precursor:->->:1497962..1498161 200 tagcaataagttaagagaatttgaacgaattgaagggtcgcgtttagaaaattgaattggttaagaaaaatgcacaatcactaaaaattaaaaagtgcggtagcgtttttcaatgcctaaaaacacctagaacacccaccgcacttttttgtatgctaaaacacacttacgcgtgtgcaacaatcaaaacaaaattttta Intra-Species Hit: Count: 1 Min: 1 Max: 200 Len: 200 Subject: aact_AA02199_AA02201|hypothetical protein:transferrin-binding protein 1 precursor|POSITIVE:POSITIVE|[1497962,1498161]|200 HSP 1 e-value: 1.0E-109 bit: 396.0 Len: 200 Query Start:1 Query End:200 Subject Strand: POSITIVE Subject Start: 1 Subject End: 200 tagcaataagttaagagaatttgaacgaattgaagggtcgcgtttagaaaattgaattggttaagaaaaatgcacaatcactaaaaattaaaaagtgcggtagcgtttttcaatgcctaaaaacacctagaacacccaccgcacttttttgtatgctaaaacacacttacgcgtgtgcaacaatcaaaacaaaattttta tagcaataagttaagagaatttgaacgaattgaagggtcgcgtttagaaaattgaattggttaagaaaaatgcacaatcactaaaaattaaaaagtgcggtagcgtttttcaatgcctaaaaacacctagaacacccaccgcacttttttgtatgctaaaacacacttacgcgtgtgcaacaatcaaaacaaaattttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCAAUAAGUUAAGAGAAUUUGAACGAAUUGAAGGGUCGCGUUUAGAAAAUUGAAUUGGUUAAGAAAAAUGCACAAUCACUAAAAAUUAAAAAGUGCGGUAGCGUUUUUCAAUGCCUAAAAACACCUAGAACACCCACCGCACUUUUUUGUAUGCUAAAACACACUUACGCGUGUGCAACAAUCAAAACAAAAUUUUUA ((((((((((..(((..(((((....)))))...((((....(((((...........(((...(((((((((((...((((...........))))..)).)))))))))...))).........)))))..))))......))).))))).)))))...(((((......)))))....................... (-38.15) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAAUUUUGUUUUGAUUGUUGCACACGCGUAAGUGUGUUUUAGCAUACAAAAAAGUGCGGUGGGUGUUCUAGGUGUUUUUAGGCAUUGAAAAACGCUACCGCACUUUUUAAUUUUUAGUGAUUGUGCAUUUUUCUUAACCAAUUCAAUUUUCUAAACGCGACCCUUCAAUUCGUUCAAAUUCUCUUAACUUAUUGCUA ..........(..((((..(((((.(((((....))))).....(((((..((((((((((((((.((((...((((((....))))))....)))))))))))))))))).(((......))))))))..............................)))))...))))..).......................... (-47.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 466 150 102 199 152 aact:AA01471|5end_type_I_restriction_enzyme_M_(modification_chain)_987321..987551_POSITIVE 434 149 102 160 114 aact:AA02673|5end_gluconate_utilization_operon_repressor_1858913..1859143_POSITIVE 412 164 121 184 143 aact:AA01918|5end_anaerobic_C4-dicarboxylate_transporter_1290931..1291161_POSITIVE 399 146 104 43 1 aact:AA01770|5end_hypothetical_protein_1193111..1193341_POSITIVE 398 148 103 113 67 aact:AA00160|5end_pilA-like_biogenesis_protein_/_prepilin_peptidase_dependent_proteinD_112836..113066_POSITIVE 391 149 103 185 131 aact:AA01824|5end_iron(III)_ABC_transporter,_ATP-binding_protein_1227705..1227935_POSITIVE 385 149 104 146 101 aact:AA02449|5end_hypothetical_protein_1687575..1687805_POSITIVE 375 148 102 181 126 aact:AA00561|5end_DNA_transformation_protein_384584..384814_POSITIVE 373 156 116 50 6 aact:AA01660|5end_hypothetical_protein_1125035..1125265_POSITIVE 349 180 135 174 137 aact:AA02872|5end_conserved_hypothetical_protein_2015539..2015769_POSITIVE 342 149 118 43 12 aact:AA01823|5end_hypothetical_protein_1227563..1227793_POSITIVE 341 150 102 177 130 aact:AA00819|5end_D-alanyl-D-alanine_carboxypeptidase_562644..562874_POSITIVE 336 148 119 110 81 aact:AA01008|5end_mazG_beta-lactamase_regulatory_protein_homolog_703470..703700_POSITIVE 325 178 133 175 125 aact:AA02196|5end_oxaloacetate_decarboxylase_beta_chain_1496308..1496538_POSITIVE 325 146 105 50 14 aact:AA01517|5end_peptidyl-prolyl_cis-trans_isomerase;_macrophage_infectivity_protein_1020989..1021219_POSITIVE 325 152 115 196 159 aact:AA01095|5end_conserved_hypothetical_protein_756327..756557_POSITIVE 323 149 101 180 129 aact:AA00174|5end_ribosomal_protein_L11_methyltransferase_123072..123302_POSITIVE 323 149 101 76 25 aact:AA00173|5end_hypothetical_protein_122968..123198_POSITIVE 322 150 104 140 92 aact:AA02695|5end_hydrogenase-3_component_G_1878565..1878795_POSITIVE 321 136 92 198 149 aact:AA02177|5end_conserved_hypothetical_protein_1488963..1489193_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 434 149 102 84 38 aact:AA02671|3end_gluconokinase_1858917..1859067_POSITIVE 398 148 103 132 86 aact:AA00158|3end_ampD_signalling_protein_(N-acetylmuramyl-L-alanine_amidase)_112935..113085_POSITIVE 391 149 103 147 93 aact:AA01823|3end_hypothetical_protein_1227747..1227897_POSITIVE 391 149 103 71 17 aact:AA01822|3end_lysostaphin_precursor_1227671..1227821_POSITIVE 385 149 104 74 29 aact:AA02446|3end_conserved_hypothetical_protein_1687583..1687733_POSITIVE 375 148 102 108 53 aact:AA00560|3end_RNA_polymerase_sigma-32_factor_384591..384741_POSITIVE 349 180 135 107 70 aact:AA02870|3end_conserved_hypothetical_protein_2015552..2015702_POSITIVE 345 150 114 88 53 aact:AA02697|3end_hydrogenase_3_maturation_protease_1880408..1880558_POSITIVE 341 139 94 58 1 aact:AA01945|3end_selenophosphate_synthase_1316022..1316172_POSITIVE 333 157 113 88 44 aact:AA02865|3end_iron-regulated_outer_membrane_protein_(omp64)_2012542..2012692_POSITIVE 325 152 115 150 113 aact:AA01092|3end_hypothetical_protein_756361..756511_POSITIVE 322 150 104 140 92 aact:AA02693|3end_hydrogenase-4_component_H_1878645..1878795_POSITIVE 321 136 92 121 72 aact:AA02175|3end_anthranilate_synthase_component_II_1488966..1489116_POSITIVE 321 148 104 150 111 aact:AA01605|3end_hypothetical_protein_1085654..1085804_POSITIVE 320 179 136 100 48 aact:AA01289|3end_hypothetical_protein_872848..872998_POSITIVE 318 149 103 92 36 aact:AA01301|3end_competence_protein_B_882095..882245_POSITIVE 316 157 112 131 85 aact:AA02731|3end_outer_membrane_lipoprotein_carrier_precursor_1912192..1912342_POSITIVE 312 176 138 42 5 aact:AA00757|3end_aerobic_respiration_sensor-response_protein_520539..520689_POSITIVE 312 152 115 111 71 aact:AA00253|3end_hypothetical_protein_177636..177786_POSITIVE 309 159 119 44 1 aact:AA01530|3end_conserved_hypothetical_protein_1028749..1028899_POSITIVE