Origin IGS: taggttgaggaaaagtgcggttggtttttatgaataaattataatttcaaataataaatggaagacaaatttatacacttacatcttccattacttcagaaataaaaattacgtatcatattttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaaatatgatacgtaatttttatttctgaagtaatggaagatgtaagtgtataaatttgtcttccatttattatttgaaattataatttattcataaaaaccaaccgcacttttcctcaaccta Mask Tandem Repeat Region ================================================ taggttgaggaaaagtgcggttggtttttatgaataaattataatttcaaataataaatggaagacaaatttatacacttacatcttccattacttcagaaataaaaattacgtatcatattttta Find is-nt database================================================ Query_seq: AA02190:AA02191|AA02190:AA02191:hydrogenase-2 component protein:hypothetical protein:->->:1493332..1493457 126 taggttgaggaaaagtgcggttggtttttatgaataaattataatttcaaataataaatggaagacaaatttatacacttacatcttccattacttcagaaataaaaattacgtatcatattttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02190:AA02191|AA02190:AA02191:hydrogenase-2 component protein:hypothetical protein:->->:1493332..1493457 126 taggttgaggaaaagtgcggttggtttttatgaataaattataatttcaaataataaatggaagacaaatttatacacttacatcttccattacttcagaaataaaaattacgtatcatattttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02190:AA02191|AA02190:AA02191:hydrogenase-2 component protein:hypothetical protein:->->:1493332..1493457 126 taggttgaggaaaagtgcggttggtttttatgaataaattataatttcaaataataaatggaagacaaatttatacacttacatcttccattacttcagaaataaaaattacgtatcatattttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taggttgaggaaaagtgcggttggtttttatgaataaattataatttcaaataataaatggaagacaaatttatacacttacatcttccattacttcagaaataaaaattacgtatcatattttta Predict ORF larger than 30AA ================================================ taggttgaggaaaagtgcggttggtttttatgaataaattataatttcaaataataaatggaagacaaatttatacacttacatcttccattacttcagaaataaaaattacgtatcatattttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taggttgaggaaaagtgcggttggtttttatgaataaattataatttcaaataataaatggaagacaaatttatacacttacatcttccattacttcagaaataaaaattacgtatcatattttta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02190:AA02191|AA02190:AA02191:hydrogenase-2 component protein:hypothetical protein:->->:1493332..1493457 126 taggttgaggaaaagtgcggttggtttttatgaataaattataatttcaaataataaatggaagacaaatttatacacttacatcttccattacttcagaaataaaaattacgtatcatattttta Intra-Species Hit: Count: 1 Min: 1 Max: 126 Len: 126 Subject: aact_AA02190_AA02191|hydrogenase-2 component protein:hypothetical protein|POSITIVE:POSITIVE|[1493332,1493457]|126 HSP 1 e-value: 9.0E-66 bit: 250.0 Len: 126 Query Start:1 Query End:126 Subject Strand: POSITIVE Subject Start: 1 Subject End: 126 taggttgaggaaaagtgcggttggtttttatgaataaattataatttcaaataataaatggaagacaaatttatacacttacatcttccattacttcagaaataaaaattacgtatcatattttta taggttgaggaaaagtgcggttggtttttatgaataaattataatttcaaataataaatggaagacaaatttatacacttacatcttccattacttcagaaataaaaattacgtatcatattttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGUUGAGGAAAAGUGCGGUUGGUUUUUAUGAAUAAAUUAUAAUUUCAAAUAAUAAAUGGAAGACAAAUUUAUACACUUACAUCUUCCAUUACUUCAGAAAUAAAAAUUACGUAUCAUAUUUUUA .....((((((...(((((..((((((((((......(((((........))))).(((((((((..................))))))))).........)))))))))))))))....)))))) (-17.27) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAAUAUGAUACGUAAUUUUUAUUUCUGAAGUAAUGGAAGAUGUAAGUGUAUAAAUUUGUCUUCCAUUUAUUAUUUGAAAUUAUAAUUUAUUCAUAAAAACCAACCGCACUUUUCCUCAACCUA ......(((((.....((((...(((((......((((((((((..((((......))))))))))))))........)))))...))))...)))))............................ (-18.94) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 338 35 3 143 105 aact:AA02757|5end_histidinol-phosphate_aminotransferase_2_1930634..1930864_POSITIVE 331 29 3 229 200 aact:AA00452|5end_phosphomannomutase;_phosphoglucomutase_315288..315518_POSITIVE 328 31 9 127 105 aact:AA01104|5end_conserved_hypothetical_protein_761155..761385_POSITIVE 317 24 3 30 8 aact:AA02484|5end_ABC_transporter,_ATP-binding_protein_1721077..1721307_POSITIVE 310 27 1 128 102 aact:AA02700|5end_succinyl-CoA_synthase,_alpha_subunit_1883256..1883486_POSITIVE 308 27 7 214 194 aact:AA00657|5end_heat_shock_protein_70_449488..449718_POSITIVE 308 27 7 26 6 aact:AA00655|5end_hypothetical_protein_449300..449530_POSITIVE 299 30 6 159 135 aact:AA01014|5end_UTP-glucose-1-phosphate_uridylyltransferase_709469..709699_POSITIVE 296 34 10 122 99 aact:AA00250|5end_lipoate_biosynthesis_protein_A_175496..175726_POSITIVE 292 30 2 59 34 aact:AA02433|5end_hypothetical_protein_1678300..1678530_POSITIVE 290 35 9 180 153 aact:AA02261|5end_30S_ribosomal_protein_S9_1538013..1538243_POSITIVE 288 30 9 117 96 aact:AA00200|5end_thymidylate_synthase_138892..139122_POSITIVE 286 27 4 190 168 aact:AA02778|5end_hypothetical_protein_1942585..1942815_POSITIVE 285 37 13 227 200 aact:AA02736|5end_conserved_hypothetical_protein_1917350..1917580_POSITIVE 284 30 3 154 123 aact:AA01704|5end_tyrosine-specific_transport_protein_1156170..1156400_POSITIVE 283 32 4 185 157 aact:AA02257|5end_conserved_hypothetical_protein_1536518..1536748_POSITIVE 283 30 7 112 86 aact:AA01850|5end_conserved_hypothetical_protein;_possible_enzyme_of_sugar_metabolism_1243710..1243940_POSITIVE 282 29 3 201 169 aact:AA02774|5end_collagenase_prtC_homolog,_possible_protease_1936870..1937100_POSITIVE 281 31 4 166 142 aact:AA01385|5end_50S_ribosomal_protein_L9_935509..935739_POSITIVE 279 34 1 140 107 aact:AA01475|5end_conserved_hypothetical_protein_(possible_cytoplasmic_protein;_DNA_binding_protein)_990364..990594_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 338 35 3 65 27 aact:AA02756|3end_phosphoserine_aminotransferase_1930636..1930786_POSITIVE 317 40 5 83 48 aact:AA00223|3end_catalase_(hydroperoxidase;_iron_heme_axial_ligand)_155808..155958_POSITIVE 308 27 7 124 104 aact:AA00655|3end_hypothetical_protein_449478..449628_POSITIVE 299 30 6 80 56 aact:AA01013|3end_phosphomannomutase_709470..709620_POSITIVE 296 50 5 110 66 aact:AA00265|3end_transcription_termination_factor_186583..186733_POSITIVE 290 35 9 49 22 aact:AA02260|3end_pseudouridylate_synthase_I_1537962..1538112_POSITIVE 286 27 4 52 30 aact:AA02777|3end_tRNA-guanine_transglycosylase_1942527..1942677_POSITIVE 284 30 3 65 34 aact:AA01703|3end_L-ribulose-phosphate_4-epimerase_1156161..1156311_POSITIVE 281 31 4 117 93 aact:AA01384|3end_hypothetical_protein_935540..935690_POSITIVE 279 34 1 127 94 aact:AA01474|3end_hypothetical_protein_990431..990581_POSITIVE 273 32 3 42 6 aact:AA02516|3end_ferric_uptake_regulation_protein_1749781..1749931_POSITIVE 271 23 2 148 129 aact:AA02433|3end_hypothetical_protein_1678475..1678625_POSITIVE 269 30 1 106 75 aact:AA02074|3end_hypothetical_protein_1409656..1409806_POSITIVE 269 29 3 99 68 aact:AA00064|3end_ribosomal_small_subunit_pseudouridine_synthase_48946..49096_POSITIVE 268 33 5 42 13 aact:AA02246|3end_peptidase_E_1528355..1528505_POSITIVE 264 45 3 46 2 aact:AA02333|3end_conserved_hypothetical_protein_1595184..1595334_POSITIVE 264 29 3 33 8 aact:AA00263|3end_ATP-dependent_RNA_helicase_185095..185245_POSITIVE 263 29 4 27 2 aact:AA02772|3end_hypothetical_protein_1936776..1936926_POSITIVE 255 36 3 56 21 aact:AA01473|3end_type_I_restriction-modification_system_S_(specificity_subunit)_990232..990382_POSITIVE 255 29 3 49 20 aact:AA00270|3end_malonyl_coA-acyl_carrier_protein_transacylase_190361..190511_POSITIVE