Origin IGS: taaaatcgtcaaaccataatacgcacgatggcacgcatcaggagaccactgctggtagaagcattgtaatatattgatatttcaaaagtattgtacaacccaacgaaatgtaaacgatgtccttagggaaaaatgtagacg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 cgtctacatttttccctaaggacatcgtttacatttcgttgggttgtacaatacttttgaaatatcaatatattacaatgcttctaccagcagtggtctcctgatgcgtgccatcgtgcgtattatggtttgacgatttta Mask Tandem Repeat Region ================================================ taaaatcgtcaaaccataatacgcacgatggcacgcatcaggagaccactgctggtagaagcattgtaatatattgatatttcaaaagtattgtacaacccaacgaaatgtaaacgatgtccttagggaaaaatgtagacg Find is-nt database================================================ Query_seq: AA02184:AA02187|AA02184:AA02187:anthranilate isomerase; indole-3-glycerol phosphate synthase; phosphoribosylanthranilate isomerase:hypothetical protein:->->:1492602..1492742 141 taaaatcgtcaaaccataatacgcacgatggcacgcatcaggagaccactgctggtagaagcattgtaatatattgatatttcaaaagtattgtacaacccaacgaaatgtaaacgatgtccttagggaaaaatgtagacg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02184:AA02187|AA02184:AA02187:anthranilate isomerase; indole-3-glycerol phosphate synthase; phosphoribosylanthranilate isomerase:hypothetical protein:->->:1492602..1492742 141 taaaatcgtcaaaccataatacgcacgatggcacgcatcaggagaccactgctggtagaagcattgtaatatattgatatttcaaaagtattgtacaacccaacgaaatgtaaacgatgtccttagggaaaaatgtagacg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02184:AA02187|AA02184:AA02187:anthranilate isomerase; indole-3-glycerol phosphate synthase; phosphoribosylanthranilate isomerase:hypothetical protein:->->:1492602..1492742 141 taaaatcgtcaaaccataatacgcacgatggcacgcatcaggagaccactgctggtagaagcattgtaatatattgatatttcaaaagtattgtacaacccaacgaaatgtaaacgatgtccttagggaaaaatgtagacg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaatcgtcaaaccataatacgcacgatggcacgcatcaggagaccactgctggtagaagcattgtaatatattgatatttcaaaagtattgtacaacccaacgaaatgtaaacgatgtccttagggaaaaatgtagacg Predict ORF larger than 30AA ================================================ taaaatcgtcaaaccataatacgcacgatggcacgcatcaggagaccactgctggtagaagcattgtaatatattgatatttcaaaagtattgtacaacccaacgaaatgtaaacgatgtccttagggaaaaatgtagacg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaatcgtcaaaccataatacgcacgatggcacgcatcaggagaccactgctggtagaagcattgtaatatattgatatttcaaaagtattgtacaacccaacgaaatgtaaacgatgtccttagggaaaaatgtagacg Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02184:AA02187|AA02184:AA02187:anthranilate isomerase; indole-3-glycerol phosphate synthase; phosphoribosylanthranilate isomerase:hypothetical protein:->->:1492602..1492742 141 taaaatcgtcaaaccataatacgcacgatggcacgcatcaggagaccactgctggtagaagcattgtaatatattgatatttcaaaagtattgtacaacccaacgaaatgtaaacgatgtccttagggaaaaatgtagacg Intra-Species Hit: Count: 1 Min: 1 Max: 141 Len: 141 Subject: aact_AA02184_AA02187|anthranilate isomerase; indole-3-glycerol phosphate synthase; phosphoribosylanthranilate isomerase:hypothetical protein|POSITIVE:POSITIVE|[1492602,1492742]|141 HSP 1 e-value: 1.0E-74 bit: 280.0 Len: 141 Query Start:1 Query End:141 Subject Strand: POSITIVE Subject Start: 1 Subject End: 141 taaaatcgtcaaaccataatacgcacgatggcacgcatcaggagaccactgctggtagaagcattgtaatatattgatatttcaaaagtattgtacaacccaacgaaatgtaaacgatgtccttagggaaaaatgtagacg taaaatcgtcaaaccataatacgcacgatggcacgcatcaggagaccactgctggtagaagcattgtaatatattgatatttcaaaagtattgtacaacccaacgaaatgtaaacgatgtccttagggaaaaatgtagacg Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAUCGUCAAACCAUAAUACGCACGAUGGCACGCAUCAGGAGACCACUGCUGGUAGAAGCAUUGUAAUAUAUUGAUAUUUCAAAAGUAUUGUACAACCCAACGAAAUGUAAACGAUGUCCUUAGGGAAAAAUGUAGACG ......((((.....(((((((..((((((.(...((((((.((....)).))))).)..)))))))......((((....))))..)))))))....(((((.((..((....))...)).)).))).........)))) (-26.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGUCUACAUUUUUCCCUAAGGACAUCGUUUACAUUUCGUUGGGUUGUACAAUACUUUUGAAAUAUCAAUAUAUUACAAUGCUUCUACCAGCAGUGGUCUCCUGAUGCGUGCCAUCGUGCGUAUUAUGGUUUGACGAUUUUA .((((..............))))..............(((((((((((..(((...((((....))))..))))))))).......))))).((.(((.(((((((((..(....)..))))))).))...))).)).... (-27.75) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 462 80 31 160 111 aact:AA01174|5end_hypothetical_protein_804700..804930_POSITIVE 386 70 21 192 141 aact:AA00729|5end_possible_hemolysin_(TlyC-like)_500204..500434_POSITIVE 383 65 21 49 12 aact:AA00816|5end_lacZ_expression_regulator_560900..561130_POSITIVE 355 59 25 136 102 aact:AA01173|5end_VapD-homolog_803850..804080_POSITIVE 339 70 21 194 140 aact:AA02439|5end_integral_membrane_protein;_possible_ABC_transporter_permease_1683902..1684132_POSITIVE 335 63 22 60 16 aact:AA02609|5end_ABC_transporter,_ATP-binding_protein/permease_1818446..1818676_POSITIVE 331 58 14 66 12 aact:AA01306|5end_glutathione_reductase_886538..886768_POSITIVE 322 58 22 191 159 aact:AA00161|5end_pilin_B_biogenesis_protein;_fimbrial_transcription_regulation_113384..113614_POSITIVE 321 66 21 195 146 aact:AA01413|5end_conserved_hypothetical_protein_951773..952003_POSITIVE 321 62 22 58 18 aact:AA01357|5end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919133..919363_POSITIVE 321 66 23 93 38 aact:AA00652|5end_arginyl-tRNA_synthetase_(arginine--tRNA_ligase)_445718..445948_POSITIVE 318 60 13 158 105 aact:AA00980|5end_hypothetical_protein_684970..685200_POSITIVE 317 55 21 194 154 aact:AA02939|5end_phosphoserine_phosphatase_2056881..2057111_POSITIVE 312 52 14 225 180 aact:AA01255|5end_hypothetical_protein_852656..852886_POSITIVE 310 53 19 197 162 aact:AA01774|5end_5,10-methylenetetrahydrofolate_reductase_1195015..1195245_POSITIVE 310 53 19 59 24 aact:AA01773|5end_hypothetical_protein_1194877..1195107_POSITIVE 309 56 14 67 26 aact:AA02250|5end_possible_cytochrome_c-type_protein_1530125..1530355_POSITIVE 309 64 22 53 16 aact:AA01872|5end_conserved_hypothetical_protein_1258804..1259034_POSITIVE 308 60 11 210 140 aact:AA02954|5end_penicillin_tolerance_protein;_hydroxymethylbutenyl_pyrophosphate_reductase_2065610..2065840_POSITIVE 308 55 14 208 165 aact:AA01313|5end_deoxyuridinetriphosphatase;_deoxyuridine_5'-triphosphate_nucleotidohydrolase_889567..889797_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 355 59 25 53 19 aact:AA01172|3end_conserved_hypothetical_protein_803847..803997_POSITIVE 310 53 19 44 9 aact:AA01772|3end_shikimate_5-dehydrogenase_protein_1194942..1195092_POSITIVE 310 61 24 127 87 aact:AA01717|3end_fumarate_hydratase,_class_II_1162660..1162810_POSITIVE 305 57 22 66 22 aact:AA01952|3end_ABC_transporter,_substrate_binding_protein_1320155..1320305_POSITIVE 305 63 25 75 37 aact:AA01431|3end_conserved_hypothetical_protein_(possible_hydrolase)_960751..960901_POSITIVE 303 63 31 145 114 aact:AA01778|3end_aminopeptidase_A/I;_cytosol_aminopeptidase;_leucyl_aminopeptidase_1199965..1200115_POSITIVE 300 57 21 79 28 aact:AA01861|3end_phosphomethylpyrimidine_kinase_1252107..1252257_POSITIVE 297 48 2 112 62 aact:AA00486|3end_nucleoside_transporter_338026..338176_POSITIVE 295 44 7 54 24 aact:AA01422|3end_cell_division_protein_E,_ABC_system_ATP-binding_protein_956736..956886_POSITIVE 291 62 24 43 5 aact:AA02451|3end_conserved_hypothetical_protein_1688926..1689076_POSITIVE 291 67 31 128 97 aact:AA00426|3end_conserved_hypothetical_protein_288205..288355_POSITIVE 288 63 21 62 17 aact:AA00164|3end_dephospho-CoA_kinase_(dephosphocoenzyme_A_kinase)_117445..117595_POSITIVE 284 52 14 131 86 aact:AA00408|3end_SUN_protein_(FMU_protein)_273688..273838_POSITIVE 283 67 22 91 42 aact:AA00909|3end_molybdopterin-guanine_dinucleotide_biosynthesis_protein_B_627183..627333_POSITIVE 282 58 24 37 2 aact:AA01682|3end_molybdate_ABC_transporter_protein_1136442..1136592_POSITIVE 282 63 22 45 2 aact:AA01629|3end_phosphatidylglycerophosphatase_A_1102449..1102599_POSITIVE 277 70 22 111 55 aact:AA00257|3end_rod-shape-determining_protein_180904..181054_POSITIVE 277 50 3 76 15 aact:AA00241|3end_formate_acetyltransferase_activating_enzyme_(anaerobic_ribonucleoside-triphosphate_reductase_activating_protein)_172090..172240_POSITIVE 276 40 6 147 110 aact:AA01890|3end_cell-cycle_protein_1275967..1276117_POSITIVE 274 57 21 85 45 aact:AA01829|3end_hypothetical_protein_1231662..1231812_POSITIVE