Origin IGS: taagccgggttctgtcgtgggcaatcattcatctaggcgcacatttacacatacgctcaagcaacctacccgaatcctgaacgggccattcattggattcctatttggtcttgctacgagtggagtttaccctgctgccaaccgttaccggcggcacggtgcgctcttaccgcaccctttcacccttacctgatctcttgcgagccatcggcggtttgctctctgctgcacttgtcgtaggctctcgccccccggacgttatccggcactctgccctgtgtagcccggactttcctcccgcctacttgtcccccggtttacgcttgcgcgacgttaggggcttcaatagaccggcgattgcctgaccaactcaggcgcgcagtatagcctaaattcttggcgaaatatagtgttaagtaaaaagttaggctagaatgagcgccacaaaaaacgttccgaaaacaaaccgcactttccccgcgctaataaggaaaccgca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgcggtttccttattagcgcggggaaagtgcggtttgttttcggaacgttttttgtggcgctcattctagcctaactttttacttaacactatatttcgccaagaatttaggctatactgcgcgcctgagttggtcaggcaatcgccggtctattgaagcccctaacgtcgcgcaagcgtaaaccgggggacaagtaggcgggaggaaagtccgggctacacagggcagagtgccggataacgtccggggggcgagagcctacgacaagtgcagcagagagcaaaccgccgatggctcgcaagagatcaggtaagggtgaaagggtgcggtaagagcgcaccgtgccgccggtaacggttggcagcagggtaaactccactcgtagcaagaccaaataggaatccaatgaatggcccgttcaggattcgggtaggttgcttgagcgtatgtgtaaatgtgcgcctagatgaatgattgcccacgacagaacccggctta Mask Tandem Repeat Region ================================================ taagccgggttctgtcgtgggcaatcattcatctaggcgcacatttacacatacgctcaagcaacctacccgaatcctgaacgggccattcattggattcctatttggtcttgctacgagtggagtttaccctgctgccaaccgttaccggcggcacggtgcgctcttaccgcaccctttcacccttacctgatctcttgcgagccatcggcggtttgctctctgctgcacttgtcgtaggctctcgccccccggacgttatccggcactctgccctgtgtagcccggactttcctcccgcctacttgtcccccggtttacgcttgcgcgacgttaggggcttcaatagaccggcgattgcctgaccaactcaggcgcgcagtatagcctaaattcttggcgaaatatagtgttaagtaaaaagttaggctagaatgagcgccacaaaaaacgttccgaaaacaaaccgcactttccccgcgctaataaggaaaccgca Find is-nt database================================================ Query_seq: AA02169:AA02171|AA02169:AA02171:hypothetical protein:polysialic acid capsule expression protein:->->:1484725..1485223 499 taagccgggttctgtcgtgggcaatcattcatctaggcgcacatttacacatacgctcaagcaacctacccgaatcctgaacgggccattcattggattcctatttggtcttgctacgagtggagtttaccctgctgccaaccgttaccggcggcacggtgcgctcttaccgcaccctttcacccttacctgatctcttgcgagccatcggcggtttgctctctgctgcacttgtcgtaggctctcgccccccggacgttatccggcactctgccctgtgtagcccggactttcctcccgcctacttgtcccccggtttacgcttgcgcgacgttaggggcttcaatagaccggcgattgcctgaccaactcaggcgcgcagtatagcctaaattcttggcgaaatatagtgttaagtaaaaagttaggctagaatgagcgccacaaaaaacgttccgaaaacaaaccgcactttccccgcgctaataaggaaaccgca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02169:AA02171|AA02169:AA02171:hypothetical protein:polysialic acid capsule expression protein:->->:1484725..1485223 499 taagccgggttctgtcgtgggcaatcattcatctaggcgcacatttacacatacgctcaagcaacctacccgaatcctgaacgggccattcattggattcctatttggtcttgctacgagtggagtttaccctgctgccaaccgttaccggcggcacggtgcgctcttaccgcaccctttcacccttacctgatctcttgcgagccatcggcggtttgctctctgctgcacttgtcgtaggctctcgccccccggacgttatccggcactctgccctgtgtagcccggactttcctcccgcctacttgtcccccggtttacgcttgcgcgacgttaggggcttcaatagaccggcgattgcctgaccaactcaggcgcgcagtatagcctaaattcttggcgaaatatagtgttaagtaaaaagttaggctagaatgagcgccacaaaaaacgttccgaaaacaaaccgcactttccccgcgctaataaggaaaccgca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02169:AA02171|AA02169:AA02171:hypothetical protein:polysialic acid capsule expression protein:->->:1484725..1485223 499 taagccgggttctgtcgtgggcaatcattcatctaggcgcacatttacacatacgctcaagcaacctacccgaatcctgaacgggccattcattggattcctatttggtcttgctacgagtggagtttaccctgctgccaaccgttaccggcggcacggtgcgctcttaccgcaccctttcacccttacctgatctcttgcgagccatcggcggtttgctctctgctgcacttgtcgtaggctctcgccccccggacgttatccggcactctgccctgtgtagcccggactttcctcccgcctacttgtcccccggtttacgcttgcgcgacgttaggggcttcaatagaccggcgattgcctgaccaactcaggcgcgcagtatagcctaaattcttggcgaaatatagtgttaagtaaaaagttaggctagaatgagcgccacaaaaaacgttccgaaaacaaaccgcactttccccgcgctaataaggaaaccgca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 13 Min: 2 Max: 385 Len: 384 Subject: UniRef90_Q5ZUQ1 Cluster: Hypothetical protein; n=1; Legionella pneumophila subsp. pneumophila str. Philadelphia 1|Rep: Hypothetical protein - Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 /ATCC 33152 / DSM 7513) HSP 1 e-value: 2.0E-21 bit: 63.5 Len: 159 Query Start:141 Query End:299 Subject Strand: null Subject Start: 86 Subject End: 139 ............................................................................................................................................ T V T G G T V R S Y R T L S P L P D L L R A - I G G L L S A A L V V G S R P P D V I R H S A L C S P D F P P ........................................................................................................................................................................................................ ............................................................................................................................................ T V T N R A V R S Y R T I S P L P T I P K G K V G G I F S V A L S V G S H L P G V T W H S A L W S P D F P P ........................................................................................................................................................................................................ HSP 2 e-value: 2.0E-21 bit: 60.8 Len: 135 Query Start:2 Query End:136 Subject Strand: null Subject Start: 34 Subject End: 84 . K P G S V V G N H S S R R T F T H T L K Q P T R I L N G P F I - - - - - - G F L F G L A T S G V Y P A ........................................................................................................................................................................................................................................................................................................................................................................... . K P G S V V G N H S S G T C V T T C L K R P T R I P Y G P Y V R T L Q S N G F L F G L A P S G V F P A ........................................................................................................................................................................................................................................................................................................................................................................... Subject: UniRef90_Q8DEK1 Cluster: Hypothetical protein; n=1; Vibrio vulnificus|Rep: Hypothetical protein - Vibrio vulnificus HSP 1 e-value: 7.0E-20 bit: 98.6 Len: 258 Query Start:93 Query End:350 Subject Strand: null Subject Start: 1 Subject End: 91 ............................................................................................ L D S Y L V L L R V E F T L L P T V T G G T V R S Y R T L S P L P D L L - - - - R A I G G L L S A A L V V G S R P P D V I R H S A L C S P D F P P A Y L S P - G L R L R D V R G F N R ..................................................................................................................................................... ............................................................................................ M E A Y L V L L R V E F T L L R T V A S R P V R S Y R T L S P L P D P L I L R I K A I G G L L S V A L V V G L R P P G V T W H P A L W S P D F P P L C Q S P E G R Q R Q S P K G L Q R ..................................................................................................................................................... Subject: UniRef90_Q0I3E8 Cluster: Hypothetical protein; n=1; Haemophilus somnus 129PT|Rep: Hypothetical protein - Haemophilus somnus (strain 129Pt) (Histophilus somni (strain 129Pt)) HSP 1 e-value: 1.0E-19 bit: 97.8 Len: 189 Query Start:2 Query End:190 Subject Strand: null Subject Start: 9 Subject End: 71 . K P G S V V G N H S S R R T F T H T L K Q P T R I L N G P F I G F L F G L A T S G V Y P A A N R Y R R H G A L L P H P F T L T ..................................................................................................................................................................................................................................................................................................................... . K P G S V L D N H S S R R T F T H T L K Q P T R T L S G Q L I E F L F G L A T S G V Y H A I K C Y H S C G V L L P H P F T L T ..................................................................................................................................................................................................................................................................................................................... Subject: UniRef90_Q8CLV8 Cluster: Hypothetical; n=1; Yersinia pestis|Rep: Hypothetical - Yersinia pestis HSP 1 e-value: 6.0E-17 bit: 89.0 Len: 297 Query Start:2 Query End:298 Subject Strand: null Subject Start: 6 Subject End: 106 . K P G S V V G N H S S R R T F T H T L K Q P T R I L N G P F I G - - F L F G L A T S G V Y P - - - A A N R Y R R H G A L L P H P F T L T * S L A S H R R F A L C C T C R R L S P P G R Y P A L C P V * P G L S S ......................................................................................................................................................................................................... . K P G S V V D S H S S R P A I A H W L K Q P T R V Q Y G P Y H A N P Y L A L L R V E F T M P R T V A S R A V R S Y R T L S P L P D P - - - T C V G H R R F A L C C T C R R L A P P R R Y L A P C P M E P G L S S ......................................................................................................................................................................................................... Subject: UniRef90_A1AG32 Cluster: Hypothetical protein; n=2; Escherichia coli|Rep: Hypothetical protein - Escherichia coli O1:K1 / APEC HSP 1 e-value: 1.0E-13 bit: 77.8 Len: 384 Query Start:2 Query End:385 Subject Strand: null Subject Start: 22 Subject End: 145 . K P G S V V G N H S S R R T F T H T L K Q P T R I L N G P F I - - G F L F G L A T S G V Y P - - - A A N R Y R R H G A L L P H P F T L T * S L A S H R R F A L C C T C R R L S P P G R Y P A L C P V * P G L S S R L L V P R F T L A R R * G L Q * T G D C L T N S G A Q Y .................................................................................................................. . K P G S V V D S H S S R P A I A H W L K Q P T R V Q Y G P Y L M N P Y L A L L R V E F T V P R T V T S R A V R S Y R T L S P L P D P - - - A Y A G H R R F A L C C T G R G F - P P Q A L P G T L P - - - - Y G A R T F L P S A R L P R R - G R R R S G D C L V S F G A Q Y .................................................................................................................. Subject: UniRef90_Q0T0E6 Cluster: Hypothetical protein; n=1; Shigella flexneri 5 str. 8401|Rep: Hypothetical protein - Shigella flexneri serotype 5b (strain 8401) HSP 1 e-value: 1.0E-10 bit: 67.8 Len: 231 Query Start:93 Query End:323 Subject Strand: null Subject Start: 37 Subject End: 114 ............................................................................................ L D S Y L V L L R V E F T L L P T V T G G T V R S Y R T L S P L P D L L R A I G G L L S A A L V V G S R - - - P P D V I R H S A L C S P D F P P A Y L S P G L R ................................................................................................................................................................................ ............................................................................................ M N P Y L A L L R V E F T V P R T V T S R A V R S Y R T L S P L P D - - P A C T G H R R F A L C C T G R G F P P P G V T W H P A L W S P D F P P L R P S P P K R ................................................................................................................................................................................ HSP 2 e-value: 2.0E-9 bit: 63.9 Len: 270 Query Start:2 Query End:271 Subject Strand: null Subject Start: 6 Subject End: 99 . K P G S V V G N H S S R R T F T H T L K Q P T R I L N G P F I - - G F L F G L A T S G V Y P - - - A A N R Y R R H G A L L P H P F T L T * S L A S H R R F A L C C T C R R L S P P G - - R Y P A L .................................................................................................................................................................................................................................... . K P G S V V D S H S S R P A I A H W L K Q P T R V Q Y G P Y L M N P Y L A L L R V E F T V P R T V T S R A V R S Y R T L S P L P D P - - - A C T G H R R F A L C C T G R G F P P P G V T W H P A L .................................................................................................................................................................................................................................... Subject: UniRef90_UPI0000167E56 Cluster: hypothetical protein SO0301.1; n=1; Shewanella oneidensis MR-1|Rep: hypothetical protein SO0301.1 - Shewanella oneidensis MR-1 HSP 1 e-value: 2.0E-10 bit: 51.6 Len: 96 Query Start:96 Query End:191 Subject Strand: null Subject Start: 41 Subject End: 72 ............................................................................................... D S Y L V L L R V E F T L L P T V T G G T V R S Y R T L S P L P .................................................................................................................................................................................................................................................................................................................... ............................................................................................... E P Y L V L L R V E F T L L R T I A S R T V R S Y R T L S P L P .................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 2.0E-10 bit: 35.8 Len: 87 Query Start:2 Query End:88 Subject Strand: null Subject Start: 9 Subject End: 37 . K P G S V V G N H S S R R T F T H T L K Q P T R I L N G P ........................................................................................................................................................................................................................................................................................................................................................................................................................... . K P G P V V D S H S S R P A I A H R L K R P T R F Q R E P ........................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: UniRef90_Q1YSE1 Cluster: Hypothetical protein; n=1; gamma proteobacterium HTCC2207|Rep: Hypothetical protein - gamma proteobacterium HTCC2207 HSP 1 e-value: 4.0E-10 bit: 66.2 Len: 303 Query Start:2 Query End:304 Subject Strand: null Subject Start: 13 Subject End: 114 . K P G S V V G N H S S R R T F T H T L K Q P T R I L N G P - - F I G F L F G L A T S G V Y P A A N R Y R R H G A L L P - H P F T L T * S L A S H R R F A L C C T C R R L S P P G R Y P A L C P V * P G L S S R L ................................................................................................................................................................................................... . K P G S V L D S H S S G T N V A V C L K Q P T R S Q R G T R H M E T Y L V L L R V G F T L P L L L P A A R C A L T A P F H P Y L C - - S R T N H R R S S L C C T G R R L S P P R C Y L A L Y P V E P G L S S A L ................................................................................................................................................................................................... Subject: UniRef90_Q1MYB0 Cluster: Hypothetical protein; n=1; Oceanobacter sp. RED65|Rep: Hypothetical protein - Oceanobacter sp. RED65 HSP 1 e-value: 2.0E-9 bit: 63.9 Len: 93 Query Start:100 Query End:192 Subject Strand: null Subject Start: 52 Subject End: 82 ................................................................................................... P I W S C Y E W S L P C C Q P L P A A R C A L T A P F H P Y L ................................................................................................................................................................................................................................................................................................................... ................................................................................................... P I W S C S E W G L P C R V P L P D A R C A L T A P F H P Y L ................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 2.0E-8 bit: 60.8 Len: 297 Query Start:2 Query End:298 Subject Strand: null Subject Start: 15 Subject End: 118 . K P G S V V - - - - G N H S S R R T F T H T L K Q P T R I L N G P F I G F L F G L A T S G V Y P A A N R Y R R H G A L L P H P F - T L T * S L A S H R R F A L C C T C R R L S P P G R Y P A L C P V * P G L S S ......................................................................................................................................................................................................... . K P G S V P C E Q G H D H S S R L W I A P K L K Q P T R S Q C G P H L R E P I W S C S E W G L P C R V P L P D A R C A L T A P F H P Y L C Y L N S H R R Y T L C C T G R G L T P P R R Y L A P C P M E P G L S S ......................................................................................................................................................................................................... Subject: UniRef90_A3WNE3 Cluster: Hypothetical protein; n=1; Idiomarina baltica OS145|Rep: Hypothetical protein - Idiomarina baltica OS145 HSP 1 e-value: 3.0E-9 bit: 63.5 Len: 141 Query Start:159 Query End:299 Subject Strand: null Subject Start: 1 Subject End: 47 .............................................................................................................................................................. V R S Y R T L S P L P D L L R A I G G L L S A A L V V G S R P P D V I R H S A L C S P D F P P ........................................................................................................................................................................................................ .............................................................................................................................................................. M R S Y R T L S P L P E P Q A A I G G L L S V A L V V S F H C P G V T W H S A L W S P D F P P ........................................................................................................................................................................................................ Subject: UniRef90_A4BFS3 Cluster: Hypothetical protein; n=1; Reinekea sp. MED297|Rep: Hypothetical protein - Reinekea sp. MED297 HSP 1 e-value: 3.0E-9 bit: 63.5 Len: 165 Query Start:145 Query End:309 Subject Strand: null Subject Start: 14 Subject End: 66 ................................................................................................................................................ L P A A R C A L T A P F H P Y L I S C E P S A V C S L L H L S * A L A P R T L S G T L P C V A R T F L P P T C .............................................................................................................................................................................................. ................................................................................................................................................ L P V S R C A L T A P F H P Y - - Q A R S L A V Y S L L H W P S A R A A Q T L S G T L P Y G A R T F L P L Q C .............................................................................................................................................................................................. Subject: UniRef90_Q5F5H4 Cluster: Hypothetical protein; n=2; Neisseria|Rep: Hypothetical protein - Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) HSP 1 e-value: 3.0E-9 bit: 63.2 Len: 204 Query Start:56 Query End:259 Subject Strand: null Subject Start: 2 Subject End: 70 ....................................................... L K Q P T R I L N G P F I G F L F G L A T S G V Y P A A N R Y R R H G A L L P H P F T L T * S L - A S H R R F A L C C T C R R L S P P G R ................................................................................................................................................................................................................................................ ....................................................... L K Q P T R T L G G Q R H C V L F G L A P N G V W P A A Y C Y Q M R G A P L P H L F T L A C A A K A A I G G F A F C S T F R R V T A P G R ................................................................................................................................................................................................................................................ Subject: UniRef90_Q57JL3 Cluster: Hypothetical; n=1; Salmonella choleraesuis|Rep: Hypothetical - Salmonella choleraesuis HSP 1 e-value: 2.0E-8 bit: 60.5 Len: 333 Query Start:2 Query End:334 Subject Strand: null Subject Start: 6 Subject End: 113 . K P G S V V G N H S S R R T F T H T L K Q P T R I L N G P F I - - G F L F G L A T S G V Y P - - - A A N R Y R R H G A L L P H P F T L T * S L A S H R R F A L C C T C R R L S P P G R Y P A L C P V * P G L S S R L L V P R F T L A R R ..................................................................................................................................................................... . K P G S V V D S H S S R P A I A H W L K Q P T R V Q Y G P Y L M N P Y L A L L R V E F T V P R T V T S R A V R S Y R T L S P L P D P - - - T C V G H R R F A L C C T G R G F - P P Q A L P G T L P - - - - Y G A R T F L P S A R L P E R ..................................................................................................................................................................... tnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagcctaaattcttggcgaaatatagtgttaagtaaaaagttaggctagaatgagcgccacaaaaaacgttccgaaaacaaaccgcactttccccgcgctaataaggaaaccgca Predict ORF larger than 30AA ================================================ Protein_Len: 58 Strand: + Start: 17 End: 190 ................ M G N H S S R R T F T H T L K Q P T R I L N G P F I G F L F G L A T S G V Y P A A N R Y R R H G A L L P H P F T L T ..................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 43 End: 237 .......................................... M Y T Y A Q A T Y P N P E R A I H W I P I W S C Y E W S L P C C Q P L P A A R C A L T A P F H P Y L I S C E P S A V C S ...................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 93 End: 362 ............................................................................................ M D S Y L V L L R V E F T L L P T V T G G T V R S Y R T L S P L P D L L R A I G G L L S A A L V V G S R P P D V I R H S ......................................................................................................................................... Protein_Len: 38 Strand: + Start: 271 End: 384 .............................................................................................................................................................................................................................................................................. M P C V A R T F L P P T C P P V Y A C A T L G A S I D R R L P D Q L R R A V ................................................................................................................... Protein_Len: 31 Strand: - Start: 170 End: 262 ......................................................................................................................................................................... R V R E G K G S R K R A M P P K S E A A S T T P E R G G S T M ............................................................................................................................................................................................................................................. tnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagcctaaattcttggcgaaatatagtgttaagtaaaaagttaggctagaatgagcgccacaaaaaacgttccgaaaacaaaccgcactttccccgcgctaataaggaaaccgca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagcctaaattcttggcgaaatatagtgttaagtaaaaagttaggctagaatgagcgccacaaaaaacgttccgaaaacaaaccgcactttccccgcgctaataaggaaaccgca Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 44 HP_score: -3.5 Tail_Score: -2.95223 Start: 200 End: 219 Full_Region: CTTACCTGATCTCTT GCGAGCC ATCGGC GGTTTGC TCTCTGCTGCACTTG .......................................................................................................................................................................................................GCGAGCCATCGGCGGTTTGC........................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 45 HP_score: -3.2 Tail_Score: -3.30635 Start: 280 End: 305 Full_Region: GGCACTCTGCCCTGT GTAGCCCGGA CTTTCC TCCCGCCTAC TTGTCCCCCGGTTTA .......................................................................................................................................................................................................................................................................................GTAGCCCGGACTTTCCTCCCGCCTAC.................................................................................................................................................................................................. Find igs database================================================ Query_seq: AA02169:AA02171|AA02169:AA02171:hypothetical protein:polysialic acid capsule expression protein:->->:1484725..1485223 499 tnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagcctaaattcttggcgaaatatagtgttaagtaaaaagttaggctagaatgagcgccacaaaaaacgttccgaaaacaaaccgcactttccccgcgctaataaggaaaccgca Intra-Species Hit: Count: 1 Min: 386 Max: 499 Len: 114 Subject: aact_AA02169_AA02171|hypothetical protein:polysialic acid capsule expression protein|POSITIVE:POSITIVE|[1484725,1485223]|499 HSP 1 e-value: 6.0E-58 bit: 226.0 Len: 114 Query Start:386 Query End:499 Subject Strand: POSITIVE Subject Start: 386 Subject End: 499 .................................................................................................................................................................................................................................................................................................................................................................................................agcctaaattcttggcgaaatatagtgttaagtaaaaagttaggctagaatgagcgccacaaaaaacgttccgaaaacaaaccgcactttccccgcgctaataaggaaaccgca .................................................................................................................................................................................................................................................................................................................................................................................................agcctaaattcttggcgaaatatagtgttaagtaaaaagttaggctagaatgagcgccacaaaaaacgttccgaaaacaaaccgcactttccccgcgctaataaggaaaccgca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGCCUAAAUUCUUGGCGAAAUAUAGUGUUAAGUAAAAAGUUAGGCUAGAAUGAGCGCCACAAAAAACGUUCCGAAAACAAACCGCACUUUCCCCGCGCUAAUAAGGAAACCGCA (((((((...(((((((........))))))).......))))))).....(((((..........)))))............((..(((((............)))))..)). (-21.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGCGGUUUCCUUAUUAGCGCGGGGAAAGUGCGGUUUGUUUUCGGAACGUUUUUUGUGGCGCUCAUUCUAGCCUAACUUUUUACUUAACACUAUAUUUCGCCAAGAAUUUAGGCU ...............(((((.(.(((((.(((.((((....)))).)))))))).).)))))......(((((((.(((((....................))))).))))))) (-23.65) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 464 90 42 123 82 aact:AA02252|5end_conserved_hypothetical_protein_1533840..1534070_POSITIVE 402 98 51 149 95 aact:AA00409|5end_methionyl-tRNA_formyltransferase_273611..273841_POSITIVE 389 90 43 150 101 aact:AA01111|5end_conserved_hypothetical_protein_764765..764995_POSITIVE 387 90 41 142 85 aact:AA01416|5end_2C-methyl-D-erythritol_2,4-cyclodiphosphate_952780..953010_POSITIVE 366 101 67 57 22 aact:AA00323|5end_conserved_hypothetical_protein_(possible_SAM-dependent_methyltransferase)_219387..219617_POSITIVE 358 100 54 213 161 aact:AA03000|5end_uracil-DNA_glycosylase_(UDG)_2095076..2095306_POSITIVE 350 106 62 137 93 aact:AA01936|5end_tyrosyl-tRNA_synthetase_1307043..1307273_POSITIVE 346 99 55 205 158 aact:AA00268|5end_acyl_carrier_protein_(ACP)_188104..188334_POSITIVE 346 99 55 54 7 aact:AA00267|5end_hypothetical_protein_187953..188183_POSITIVE 344 90 43 122 74 aact:AA00792|5end_conserved_hypothetical_protein_543142..543372_POSITIVE 342 101 62 146 103 aact:AA01532|5end_hypothetical_protein_1028771..1029001_POSITIVE 342 101 62 80 37 aact:AA01531|5end_hypothetical_protein_1028705..1028935_POSITIVE 340 97 54 224 174 aact:AA00347|5end_50S_ribosomal_protein_L11_232137..232367_POSITIVE 339 99 51 190 129 aact:AA02668|5end_gluconate_permease_1856758..1856988_POSITIVE 339 107 61 204 155 aact:AA00679|5end_cytoplasmic_axial_filament_(Ribonuclease_G)_463288..463518_POSITIVE 337 90 43 137 88 aact:AA02410|5end_cell_division_protein_1655429..1655659_POSITIVE 334 90 47 176 127 aact:AA00212|5end_ribokinase_146056..146286_POSITIVE 334 90 47 75 26 aact:AA00211|5end_hypothetical_protein_145955..146185_POSITIVE 329 106 61 139 93 aact:AA01603|5end_oligopeptidase_A_1082898..1083128_POSITIVE 329 95 51 137 85 aact:AA01305|5end_conserved_hypothetical_protein_885598..885828_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 464 90 42 46 5 aact:AA02251|3end_biotin_sulfoxide_reductase;_Trimethylamine-N-oxide_reductase_precursor_1533843..1533993_POSITIVE 402 98 51 146 92 aact:AA00408|3end_SUN_protein_(FMU_protein)_273688..273838_POSITIVE 387 90 41 142 85 aact:AA01413|3end_conserved_hypothetical_protein_952860..953010_POSITIVE 342 101 62 44 1 aact:AA01530|3end_conserved_hypothetical_protein_1028749..1028899_POSITIVE 340 97 54 65 15 aact:AA00345|3end_transcription_antitermination_protein_232058..232208_POSITIVE 339 107 61 84 35 aact:AA00675|3end_glutaredoxin_2_463248..463398_POSITIVE 334 90 47 72 23 aact:AA00209|3end_D-ribose_ABC_transporter,_periplasmic-binding_protein_146032..146182_POSITIVE 330 90 44 57 13 aact:AA00010|3end_transposase_orfB_fragment_6297..6447_POSITIVE 322 90 44 79 33 aact:AA00578|3end_DNA_topoisomerase_395201..395351_POSITIVE 321 106 61 127 60 aact:AA00973|3end_transcriptional_regulator,_DeoR_family_679237..679387_POSITIVE 320 90 42 46 5 aact:AA02142|3end_outer_membrane_protein_OprM_precursor_1467532..1467682_POSITIVE 319 90 62 30 2 aact:AA01109|3end_oxidoreductase_(possible_short-chain_alcohol_dehydrogenase)_764725..764875_POSITIVE 316 99 61 99 55 aact:AA02940|3end_conserved_hypothetical_protein_2058652..2058802_POSITIVE 316 99 57 97 53 aact:AA01992|3end_protease_IV;_signal_peptide_peptidase;_Endopeptidase_IV_1355004..1355154_POSITIVE 315 97 55 49 4 aact:AA01830|3end_2,3-cyclic-nucleotide_2-phosphodiesterase_1233609..1233759_POSITIVE 309 90 43 108 58 aact:AA01217|3end_hypothetical_protein_832855..833005_POSITIVE 309 107 61 87 44 aact:AA00470|3end_conserved_hypothetical_protein_(possible_Zn-dependent_protease_with_chaperone_function)_327659..327809_POSITIVE 305 99 53 137 85 aact:AA02731|3end_outer_membrane_lipoprotein_carrier_precursor_1912192..1912342_POSITIVE 305 97 53 145 90 aact:AA00500|3end_hypothetical_protein_347356..347506_POSITIVE 305 97 53 68 13 aact:AA00499|3end_pyridoxamine-5'-phosphate_oxidase_347279..347429_POSITIVE