Origin IGS: aaaacgttgttttttgtgaccgcactttttgtcctgcaggaaattttagtgctgaatctattaagctatttttcatagacaaaaaaatagaggcttcaaaagcctatattccgtatcggtaaaatgaaatttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaatttcattttaccgatacggaatataggcttttgaagcctctatttttttgtctatgaaaaatagcttaatagattcagcactaaaatttcctgcaggacaaaaagtgcggtcacaaaaaacaacgtttt Mask Tandem Repeat Region ================================================ aaaacgttgttttttgtgaccgcactttttgtcctgcaggaaattttagtgctgaatctattaagctatttttcatagacaaaaaaatagaggcttcaaaagcctatattccgtatcggtaaaatgaaatttta Find is-nt database================================================ Query_seq: AA02167:AA02168|AA02167:AA02168:hypothetical protein:possible protease; heat shock protein:->->:1483535..1483668 134 aaaacgttgttttttgtgaccgcactttttgtcctgcaggaaattttagtgctgaatctattaagctatttttcatagacaaaaaaatagaggcttcaaaagcctatattccgtatcggtaaaatgaaatttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02167:AA02168|AA02167:AA02168:hypothetical protein:possible protease; heat shock protein:->->:1483535..1483668 134 aaaacgttgttttttgtgaccgcactttttgtcctgcaggaaattttagtgctgaatctattaagctatttttcatagacaaaaaaatagaggcttcaaaagcctatattccgtatcggtaaaatgaaatttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02167:AA02168|AA02167:AA02168:hypothetical protein:possible protease; heat shock protein:->->:1483535..1483668 134 aaaacgttgttttttgtgaccgcactttttgtcctgcaggaaattttagtgctgaatctattaagctatttttcatagacaaaaaaatagaggcttcaaaagcctatattccgtatcggtaaaatgaaatttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaaacgttgttttttgtgaccgcactttttgtcctgcaggaaattttagtgctgaatctattaagctatttttcatagacaaaaaaatagaggcttcaaaagcctatattccgtatcggtaaaatgaaatttta Predict ORF larger than 30AA ================================================ aaaacgttgttttttgtgaccgcactttttgtcctgcaggaaattttagtgctgaatctattaagctatttttcatagacaaaaaaatagaggcttcaaaagcctatattccgtatcggtaaaatgaaatttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaacgttgttttttgtgaccgcactttttgtcctgcaggaaattttagtgctgaatctattaagctatttttcatagacaaaaaaatagaggcttcaaaagcctatattccgtatcggtaaaatgaaatttta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02167:AA02168|AA02167:AA02168:hypothetical protein:possible protease; heat shock protein:->->:1483535..1483668 134 aaaacgttgttttttgtgaccgcactttttgtcctgcaggaaattttagtgctgaatctattaagctatttttcatagacaaaaaaatagaggcttcaaaagcctatattccgtatcggtaaaatgaaatttta Intra-Species Hit: Count: 1 Min: 1 Max: 134 Len: 134 Subject: aact_AA02167_AA02168|hypothetical protein:possible protease; heat shock protein|POSITIVE:POSITIVE|[1483535,1483668]|134 HSP 1 e-value: 6.0E-58 bit: 224.0 Len: 134 Query Start:1 Query End:134 Subject Strand: POSITIVE Subject Start: 1 Subject End: 134 aaaacgttgttttttgtgaccgcactttttgtcctgcaggaaattttagtgctgaatctattaagctatttttcatagacnnnnnnntagaggcttcaaaagcctatattccgtatcggtaaaatgaaatttta aaaacgttgttttttgtgaccgcactttttgtcctgcaggaaattttagtgctgaatctattaagctatttttcatagacaaaaaaatagaggcttcaaaagcctatattccgtatcggtaaaatgaaatttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAACGUUGUUUUUUGUGACCGCACUUUUUGUCCUGCAGGAAAUUUUAGUGCUGAAUCUAUUAAGCUAUUUUUCAUAGACAAAAAAAUAGAGGCUUCAAAAGCCUAUAUUCCGUAUCGGUAAAAUGAAAUUUUA ...........(((..(.((((...((((((((......((((...((((..(((.....))).))))..))))...)))))))).....(((((.....)))))...........))))...)..)))..... (-23.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAUUUCAUUUUACCGAUACGGAAUAUAGGCUUUUGAAGCCUCUAUUUUUUUGUCUAUGAAAAAUAGCUUAAUAGAUUCAGCACUAAAAUUUCCUGCAGGACAAAAAGUGCGGUCACAAAAAACAACGUUUU .....((((((......((((.((((((.(((((.....))))).))))))..)))).))))))............(((...(((((......(((....))).....))))).)))................. (-23.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 458 40 1 98 59 aact:AA01953|5end_hypothetical_protein_1320132..1320362_POSITIVE 449 47 1 155 105 aact:AA01554|5end_conserved_hypothetical_protein;_hypothetical_membrane_protein_1042061..1042291_POSITIVE 414 47 1 212 167 aact:AA02902|5end_aerobic_respiration_control_protein_2031171..2031401_POSITIVE 399 43 1 144 106 aact:AA02987|5end_histidine_triad_protein_homolog_2088767..2088997_POSITIVE 393 28 1 230 203 aact:AA02220|5end_ABC_transport_system_permease_protein_1511155..1511385_POSITIVE 373 40 1 151 112 aact:AA00409|5end_methionyl-tRNA_formyltransferase_273611..273841_POSITIVE 365 38 1 217 180 aact:AA00054|5end_cytochrome_c_biogenesis_protein_41393..41623_POSITIVE 348 40 1 131 95 aact:AA01603|5end_oligopeptidase_A_1082898..1083128_POSITIVE 342 50 1 213 161 aact:AA00218|5end_argininosuccinate_lyase_150758..150988_POSITIVE 342 50 1 111 59 aact:AA00217|5end_hypothetical_protein_150656..150886_POSITIVE 335 29 1 178 150 aact:AA00969|5end_conserved_hypothetical_protein_(possible_NADH_pyrophosphatase)_675329..675559_POSITIVE 335 50 1 200 150 aact:AA00654|5end_conserved_hypothetical_protein_447772..448002_POSITIVE 335 50 1 110 60 aact:AA00653|5end_hypothetical_protein_447682..447912_POSITIVE 327 52 16 160 122 aact:AA00419|5end_conserved_hypothetical_protein_(possible_phosphatidylethanolamine-binding_protein)_284337..284567_POSITIVE 324 47 5 195 145 aact:AA01780|5end_ABC-related_molybdenum_transport_system_permease_protein_1201084..1201314_POSITIVE 322 59 17 177 134 aact:AA01008|5end_mazG_beta-lactamase_regulatory_protein_homolog_703470..703700_POSITIVE 320 50 1 143 104 aact:AA01988|5end_dihydrolipoamide_dehydrogenase;_lipoamide_dehydrogenase_1350517..1350747_POSITIVE 320 50 1 41 2 aact:AA01987|5end_hypothetical_protein_1350415..1350645_POSITIVE 320 38 1 129 94 aact:AA01291|5end_excinuclease_ABC_subunit_A_872829..873059_POSITIVE 319 41 5 195 167 aact:AA02521|5end_conserved_hypothetical_protein_1752603..1752833_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 458 40 1 41 2 aact:AA01952|3end_ABC_transporter,_substrate_binding_protein_1320155..1320305_POSITIVE 414 47 1 99 54 aact:AA02899|3end_oligopeptide_ABC_transporter,_ATP-binding_protein_2031138..2031288_POSITIVE 399 30 1 88 59 aact:AA02913|3end_ribosomal_large_subunit_pseudouridine_synthase_2040527..2040677_POSITIVE 391 59 11 56 6 aact:AA01553|3end_conserved_hypothetical_protein_1042032..1042182_POSITIVE 373 40 1 148 109 aact:AA00408|3end_SUN_protein_(FMU_protein)_273688..273838_POSITIVE 348 55 11 114 70 aact:AA00093|3end_L-serine_dehydratase_(L-serine_deaminase)_71173..71323_POSITIVE 346 40 1 92 48 aact:AA02562|3end_adenine_specific_methylase_1785146..1785296_POSITIVE 342 50 1 97 45 aact:AA00216|3end_conserved_hypothetical_protein_150722..150872_POSITIVE 339 49 1 91 46 aact:AA00470|3end_conserved_hypothetical_protein_(possible_Zn-dependent_protease_with_chaperone_function)_327659..327809_POSITIVE 335 29 1 36 8 aact:AA00967|3end_conserved_hypothetical_protein_675267..675417_POSITIVE 334 50 1 88 30 aact:AA00010|3end_transposase_orfB_fragment_6297..6447_POSITIVE 332 35 1 139 106 aact:AA01104|3end_conserved_hypothetical_protein_761642..761792_POSITIVE 327 52 16 51 13 aact:AA00417|3end_outer_membrane_lipoprotein_precursor_(probable_D-methionine-binding_lipoprotein)_284308..284458_POSITIVE 322 59 17 73 30 aact:AA01006|3end_conserved_hypothetical_protein_(possible_cytosine_deaminas)_703446..703596_POSITIVE 320 50 1 46 7 aact:AA01986|3end_pyruvate_dehydrogenase_complex_E2_1350500..1350650_POSITIVE 320 38 1 68 33 aact:AA01289|3end_hypothetical_protein_872848..872998_POSITIVE 319 41 5 124 96 aact:AA02519|3end_DNA_gyrase_A_1752612..1752762_POSITIVE 311 44 1 110 61 aact:AA00212|3end_ribokinase_147047..147197_POSITIVE 309 58 11 107 59 aact:AA02112|3end_cell_division_protein_1442184..1442334_POSITIVE 308 67 21 76 32 aact:AA01077|3end_transposase_746870..747020_POSITIVE