Origin IGS: tagctgatgcgatcaaagtgcggtttatttctaaggtattttataacctcgctaccttcttcaactatcagataaattattccttgaattgcccaaaattatcaacccggtttgcaatgggcaaaaataaatttagttaaatggctgacgacagcttgtcaaaaccctgccgtggcagtataaataagcagatttttacacaacatctcgaggattattct .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agaataatcctcgagatgttgtgtaaaaatctgcttatttatactgccacggcagggttttgacaagctgtcgtcagccatttaactaaatttatttttgcccattgcaaaccgggttgataattttgggcaattcaaggaataatttatctgatagttgaagaaggtagcgaggttataaaataccttagaaataaaccgcactttgatcgcatcagcta Mask Tandem Repeat Region ================================================ tagctgatgcgatcaaagtgcggtttatttctaaggtattttataacctcgctaccttcttcaactatcagataaattattccttgaattgcccaaaattatcaacccggtttgcaatgggcaaaaataaatttagttaaatggctgacgacagcttgtcaaaaccctgccgtggcagtataaataagcagatttttacacaacatctcgaggattattct Find is-nt database================================================ Query_seq: AA02135:AA02136|AA02135:AA02136:hypothetical protein:aspartate aminotransferase:->->:1459128..1459348 221 tagctgatgcgatcaaagtgcggtttatttctaaggtattttataacctcgctaccttcttcaactatcagataaattattccttgaattgcccaaaattatcaacccggtttgcaatgggcaaaaataaatttagttaaatggctgacgacagcttgtcaaaaccctgccgtggcagtataaataagcagatttttacacaacatctcgaggattattct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02135:AA02136|AA02135:AA02136:hypothetical protein:aspartate aminotransferase:->->:1459128..1459348 221 tagctgatgcgatcaaagtgcggtttatttctaaggtattttataacctcgctaccttcttcaactatcagataaattattccttgaattgcccaaaattatcaacccggtttgcaatgggcaaaaataaatttagttaaatggctgacgacagcttgtcaaaaccctgccgtggcagtataaataagcagatttttacacaacatctcgaggattattct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02135:AA02136|AA02135:AA02136:hypothetical protein:aspartate aminotransferase:->->:1459128..1459348 221 tagctgatgcgatcaaagtgcggtttatttctaaggtattttataacctcgctaccttcttcaactatcagataaattattccttgaattgcccaaaattatcaacccggtttgcaatgggcaaaaataaatttagttaaatggctgacgacagcttgtcaaaaccctgccgtggcagtataaataagcagatttttacacaacatctcgaggattattct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagctgatgcgatcaaagtgcggtttatttctaaggtattttataacctcgctaccttcttcaactatcagataaattattccttgaattgcccaaaattatcaacccggtttgcaatgggcaaaaataaatttagttaaatggctgacgacagcttgtcaaaaccctgccgtggcagtataaataagcagatttttacacaacatctcgaggattattct Predict ORF larger than 30AA ================================================ Protein_Len: 42 Strand: + Start: 12 End: 137 ........... M K V R F I S K V F Y N L A T F F N Y Q I N Y S L N C P K L S T R F A M G K N K F S .................................................................................... Protein_Len: 31 Strand: + Start: 88 End: 180 ....................................................................................... M A Q N Y Q P G L Q W A K I N L V K W L T T A C Q N P A V A V ......................................... tagctgatgcgatcaaagtgcggtttatttctaaggtattttataacctcgctaccttcttcaactatcagataaattattccttgaattgcccaaaattatcaacccggtttgcaatgggcaaaaataaatttagttaaatggctgacgacagcttgtcaaaaccctgccgtggcagtataaataagcagatttttacacaacatctcgaggattattct Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 155 End: 184 ..........................................................................................................................................................CTTGTCAAAACCCTGCCGTGGCAGTATAAA..................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagctgatgcgatcaaagtgcggtttatttctaaggtattttataacctcgctaccttcttcaactatcagataaattattccttgaattgcccaaaattatcaacccggtttgcaatgggcaaaaataaatttagttaaatggctgacgacagcttgtcaaaaccctgccgtggcagtataaataagcagatttttacacaacatctcgaggattattct Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02135:AA02136|AA02135:AA02136:hypothetical protein:aspartate aminotransferase:->->:1459128..1459348 221 tagctgatgcgatcaaagtgcggtttatttctaaggtattttataacctcgctaccttcttcaactatcagataaattattccttgaattgcccaaaattatcaacccggtttgcaatgggcaaaaataaatttagttaaatggctgacgacagcttgtcaaaaccctgccgtggcagtataaataagcagatttttacacaacatctcgaggattattct Intra-Species Hit: Count: 1 Min: 1 Max: 221 Len: 221 Subject: aact_AA02135_AA02136|hypothetical protein:aspartate aminotransferase|POSITIVE:POSITIVE|[1459128,1459348]|221 HSP 1 e-value: 1.0E-122 bit: 438.0 Len: 221 Query Start:1 Query End:221 Subject Strand: POSITIVE Subject Start: 1 Subject End: 221 tagctgatgcgatcaaagtgcggtttatttctaaggtattttataacctcgctaccttcttcaactatcagataaattattccttgaattgcccaaaattatcaacccggtttgcaatgggcaaaaataaatttagttaaatggctgacgacagcttgtcaaaaccctgccgtggcagtataaataagcagatttttacacaacatctcgaggattattct tagctgatgcgatcaaagtgcggtttatttctaaggtattttataacctcgctaccttcttcaactatcagataaattattccttgaattgcccaaaattatcaacccggtttgcaatgggcaaaaataaatttagttaaatggctgacgacagcttgtcaaaaccctgccgtggcagtataaataagcagatttttacacaacatctcgaggattattct Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCUGAUGCGAUCAAAGUGCGGUUUAUUUCUAAGGUAUUUUAUAACCUCGCUACCUUCUUCAACUAUCAGAUAAAUUAUUCCUUGAAUUGCCCAAAAUUAUCAACCCGGUUUGCAAUGGGCAAAAAUAAAUUUAGUUAAAUGGCUGACGACAGCUUGUCAAAACCCUGCCGUGGCAGUAUAAAUAAGCAGAUUUUUACACAACAUCUCGAGGAUUAUUCU ...((((((.((..((.(((((...........((((........))))))).)).))..))...))))))......((.(((((((.(((((((.....(((.....)))......)))))))........((((((....)))))).....((((((.......((((....)))).....))))))..................))))))).)).... (-43.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGAAUAAUCCUCGAGAUGUUGUGUAAAAAUCUGCUUAUUUAUACUGCCACGGCAGGGUUUUGACAAGCUGUCGUCAGCCAUUUAACUAAAUUUAUUUUUGCCCAUUGCAAACCGGGUUGAUAAUUUUGGGCAAUUCAAGGAAUAAUUUAUCUGAUAGUUGAAGAAGGUAGCGAGGUUAUAAAAUACCUUAGAAAUAAACCGCACUUUGAUCGCAUCAGCUA .......((((.((...(((((.(((((....((((.((.(..((((....))))..)...)).))))....(((((((...(((......)))..(((((.....)))))...)))))))..))))).))))))).)))).........(((((.((.((..(((((.(((.(((........))).((....))...))))))))..)))))))))... (-46.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 364 185 141 61 18 aact:AA00923|5end_diaminopimelate_decarboxylase_633993..634223_POSITIVE 330 42 1 59 18 aact:AA00602|5end_1-deoxy-D-xylulose_5-phosphate_reductoisomerase_409077..409307_POSITIVE 326 176 136 188 150 aact:AA00426|5end_conserved_hypothetical_protein_288027..288257_POSITIVE 315 179 135 49 1 aact:AA01188|5end_conserved_hypothetical_protein_813912..814142_POSITIVE 311 43 3 188 148 aact:AA01083|5end_preprotein_translocase_SecA_subunit_748131..748361_POSITIVE 307 42 3 50 5 aact:AA00960|5end_possible_outer_membrane_protein_665265..665495_POSITIVE 306 124 82 156 99 aact:AA01472|5end_hypothetical_protein_988896..989126_POSITIVE 305 178 135 201 158 aact:AA01316|5end_hypothetical_protein_891262..891492_POSITIVE 303 190 142 212 157 aact:AA00993|5end_possible_D-tyrosyl-tRNA(Tyr)_deacylase_694488..694718_POSITIVE 302 50 2 132 89 aact:AA00835|5end_UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapep_tide)_575212..575442_POSITIVE 301 176 144 226 184 aact:AA01194|5end_acyl_carrier_protein_819595..819825_POSITIVE 299 55 11 85 44 aact:AA01176|5end_hypothetical_protein_806309..806539_POSITIVE 298 178 139 224 176 aact:AA02397|5end_RNA_polymerase_sigma_factor_sigma-70_1647095..1647325_POSITIVE 296 180 142 96 41 aact:AA01184|5end_hypothetical_protein_810838..811068_POSITIVE 295 30 8 152 129 aact:AA02937|5end_conserved_hypothetical_protein_2056379..2056609_POSITIVE 295 186 142 220 179 aact:AA02533|5end_zinc_protease;_peptidase_1762010..1762240_POSITIVE 294 127 81 196 151 aact:AA02006|5end_PEP--fructosephosphotransferase_system_repressor,_DeoR_family_1363930..1364160_POSITIVE 292 150 108 170 126 aact:AA01730|5end_conserved_hypothetical_protein_(possible_transcriptional_regulator)_1168725..1168955_POSITIVE 292 46 3 40 1 aact:AA00408|5end_SUN_protein_(FMU_protein)_272256..272486_POSITIVE 291 175 143 145 99 aact:AA02770|5end_hypothetical_protein_1935840..1936070_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 324 181 141 90 58 aact:AA01248|3end_conserved_hypothetical_protein_(possible_phosphoesterase)_850302..850452_POSITIVE 324 177 141 129 91 aact:AA01155|3end_hypothetical_protein_791411..791561_POSITIVE 315 179 135 49 1 aact:AA01187|3end_conserved_hypothetical_protein_813992..814142_POSITIVE 302 50 2 58 15 aact:AA00833|3end_UDP-N-acetylmuramate-alanine_ligase_575218..575368_POSITIVE 302 175 134 145 108 aact:AA00266|3end_conserved_hypothetical_protein_(probable_metabolite_transport_protein)_187946..188096_POSITIVE 299 55 11 91 50 aact:AA01175|3end_6-phosphogluconate_dehydrogenase_806395..806545_POSITIVE 296 180 142 80 25 aact:AA01183|3end_conserved_hypothetical_protein_810902..811052_POSITIVE 295 30 8 66 43 aact:AA02936|3end_lipoprotein_E_precursor;_outer_membrane_protein_P4_precursor;_acid_phosphatase_2056373..2056523_POSITIVE 295 186 142 139 98 aact:AA02532|3end_conserved_hypothetical_protein_1762009..1762159_POSITIVE 295 30 3 151 126 aact:AA01081|3end_hypothetical_protein_748189..748339_POSITIVE 294 53 14 97 61 aact:AA01778|3end_aminopeptidase_A/I;_cytosol_aminopeptidase;_leucyl_aminopeptidase_1199965..1200115_POSITIVE 291 60 15 110 41 aact:AA01966|3end_2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_pyrophosphokinase_1331514..1331664_POSITIVE 289 42 4 88 53 aact:AA01090|3end_leucyl-tRNA_synthetase,_leucyl-tRNA_ligase_756213..756363_POSITIVE 288 181 141 43 9 aact:AA01565|3end_conserved_hypothetical_protein_1050967..1051117_POSITIVE 286 43 8 82 46 aact:AA02679|3end_formate_dehydrogenase,_gamma_subunit_1865811..1865961_POSITIVE 284 199 153 144 90 aact:AA02687|3end_hydrogenase-4_component_D_1874043..1874193_POSITIVE 284 48 2 82 33 aact:AA01557|3end_recombinase_A_1045965..1046115_POSITIVE 284 58 15 97 49 aact:AA00072|3end_conserved_hypothetical_protein_54947..55097_POSITIVE 281 42 3 55 8 aact:AA00718|3end_ABC-related_ATP-binding_protein;_MRP_protein_494723..494873_POSITIVE 278 173 135 127 83 aact:AA00422|3end_LapB_membrane_protein_286480..286630_POSITIVE