Origin IGS: taaagccacgtaataactgctgattagataatgcgaaacgtgcttgaatttatgcttacacttctaaataagtcttgataaatcaatatttattgatgttacaaggctttatttcttcagttttcatcacgtttaactagtaatagtgacctttgaataactctgtgttttcattaaatcgtagtgttcatttactttgtcactaatttcgcataacatatattatgttaaataaaataaatggtaagtcacttctgggaatttgaggtttgctctctttaaaaatgactgggtaaaaaaatttatttcttgaagatttgattttttgagcgtttatgatgatgagatattcgtgagtttttagaggtaggtgaaaatacttctctagctgaatctaggtttataaaaatcgactgatattcatagagggtttttatcgttatgtacatccttccgtttccattaaataatctcttaacaatttagttacaattgagaatttatttcagtaacactacttaaatgagaattgttgttattataaaattcattttaaattaaacacttactaagatattttatgagaatttgattgactattctcaataaggtgaataaaatgcgcacaacaaattaagttgtattgcgatctattaacaaattaaggaaaggagttggtt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaccaactcctttccttaatttgttaatagatcgcaatacaacttaatttgttgtgcgcattttattcaccttattgagaatagtcaatcaaattctcataaaatatcttagtaagtgtttaatttaaaatgaattttataataacaacaattctcatttaagtagtgttactgaaataaattctcaattgtaactaaattgttaagagattatttaatggaaacggaaggatgtacataacgataaaaaccctctatgaatatcagtcgatttttataaacctagattcagctagagaagtattttcacctacctctaaaaactcacgaatatctcatcatcataaacgctcaaaaaatcaaatcttcaagaaataaatttttttacccagtcatttttaaagagagcaaacctcaaattcccagaagtgacttaccatttattttatttaacataatatatgttatgcgaaattagtgacaaagtaaatgaacactacgatttaatgaaaacacagagttattcaaaggtcactattactagttaaacgtgatgaaaactgaagaaataaagccttgtaacatcaataaatattgatttatcaagacttatttagaagtgtaagcataaattcaagcacgtttcgcattatctaatcagcagttattacgtggcttta Mask Tandem Repeat Region ================================================ taaagccacgtaataactgctgattagataatgcgaaacgtgcttgaatttatgcttacacttctaaataagtcttgataaatcaatatttattgatgttacaaggctttatttcttcagttttcatcacgtttaactagtaatagtgacctttgaataactctgtgttttcattaaatcgtagtgttcatttactttgtcactaatttcgcataacatatattatgttaaataaaataaatggtaagtcacttctgggaatttgaggtttgctctctttaaaaatgactgggtaaaaaaatttatttcttgaagatttgattttttgagcgtttatgatgatgagatattcgtgagtttttagaggtaggtgaaaatacttctctagctgaatctaggtttataaaaatcgactgatattcatagagggtttttatcgttatgtacatccttccgtttccattaaataatctcttaacaatttagttacaattgagaatttatttcagtaacactacttaaatgagaattgttgttattataaaattcattttaaattaaacacttactaagatattttatgagaatttgattgactattctcaataaggtgaataaaatgcgcacaacaaattaagttgtattgcgatctattaacaaattaaggaaaggagttggtt Find is-nt database================================================ Query_seq: AA02119:AA02120|AA02119:AA02120:hypothetical protein:nonheme ferritin:->->:1446641..1447320 680 taaagccacgtaataactgctgattagataatgcgaaacgtgcttgaatttatgcttacacttctaaataagtcttgataaatcaatatttattgatgttacaaggctttatttcttcagttttcatcacgtttaactagtaatagtgacctttgaataactctgtgttttcattaaatcgtagtgttcatttactttgtcactaatttcgcataacatatattatgttaaataaaataaatggtaagtcacttctgggaatttgaggtttgctctctttaaaaatgactgggtaaaaaaatttatttcttgaagatttgattttttgagcgtttatgatgatgagatattcgtgagtttttagaggtaggtgaaaatacttctctagctgaatctaggtttataaaaatcgactgatattcatagagggtttttatcgttatgtacatccttccgtttccattaaataatctcttaacaatttagttacaattgagaatttatttcagtaacactacttaaatgagaattgttgttattataaaattcattttaaattaaacacttactaagatattttatgagaatttgattgactattctcaataaggtgaataaaatgcgcacaacaaattaagttgtattgcgatctattaacaaattaaggaaaggagttggtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02119:AA02120|AA02119:AA02120:hypothetical protein:nonheme ferritin:->->:1446641..1447320 680 taaagccacgtaataactgctgattagataatgcgaaacgtgcttgaatttatgcttacacttctaaataagtcttgataaatcaatatttattgatgttacaaggctttatttcttcagttttcatcacgtttaactagtaatagtgacctttgaataactctgtgttttcattaaatcgtagtgttcatttactttgtcactaatttcgcataacatatattatgttaaataaaataaatggtaagtcacttctgggaatttgaggtttgctctctttaaaaatgactgggtaaaaaaatttatttcttgaagatttgattttttgagcgtttatgatgatgagatattcgtgagtttttagaggtaggtgaaaatacttctctagctgaatctaggtttataaaaatcgactgatattcatagagggtttttatcgttatgtacatccttccgtttccattaaataatctcttaacaatttagttacaattgagaatttatttcagtaacactacttaaatgagaattgttgttattataaaattcattttaaattaaacacttactaagatattttatgagaatttgattgactattctcaataaggtgaataaaatgcgcacaacaaattaagttgtattgcgatctattaacaaattaaggaaaggagttggtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02119:AA02120|AA02119:AA02120:hypothetical protein:nonheme ferritin:->->:1446641..1447320 680 taaagccacgtaataactgctgattagataatgcgaaacgtgcttgaatttatgcttacacttctaaataagtcttgataaatcaatatttattgatgttacaaggctttatttcttcagttttcatcacgtttaactagtaatagtgacctttgaataactctgtgttttcattaaatcgtagtgttcatttactttgtcactaatttcgcataacatatattatgttaaataaaataaatggtaagtcacttctgggaatttgaggtttgctctctttaaaaatgactgggtaaaaaaatttatttcttgaagatttgattttttgagcgtttatgatgatgagatattcgtgagtttttagaggtaggtgaaaatacttctctagctgaatctaggtttataaaaatcgactgatattcatagagggtttttatcgttatgtacatccttccgtttccattaaataatctcttaacaatttagttacaattgagaatttatttcagtaacactacttaaatgagaattgttgttattataaaattcattttaaattaaacacttactaagatattttatgagaatttgattgactattctcaataaggtgaataaaatgcgcacaacaaattaagttgtattgcgatctattaacaaattaaggaaaggagttggtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaagccacgtaataactgctgattagataatgcgaaacgtgcttgaatttatgcttacacttctaaataagtcttgataaatcaatatttattgatgttacaaggctttatttcttcagttttcatcacgtttaactagtaatagtgacctttgaataactctgtgttttcattaaatcgtagtgttcatttactttgtcactaatttcgcataacatatattatgttaaataaaataaatggtaagtcacttctgggaatttgaggtttgctctctttaaaaatgactgggtaaaaaaatttatttcttgaagatttgattttttgagcgtttatgatgatgagatattcgtgagtttttagaggtaggtgaaaatacttctctagctgaatctaggtttataaaaatcgactgatattcatagagggtttttatcgttatgtacatccttccgtttccattaaataatctcttaacaatttagttacaattgagaatttatttcagtaacactacttaaatgagaattgttgttattataaaattcattttaaattaaacacttactaagatattttatgagaatttgattgactattctcaataaggtgaataaaatgcgcacaacaaattaagttgtattgcgatctattaacaaattaaggaaaggagttggtt Predict ORF larger than 30AA ================================================ Protein_Len: 59 Strand: - Start: 306 End: 482 ................................................................................................................................................................................................................................................................................................................. K K F I Q N K S R K H H H S I R S N K S T P S F V E R A S D L N I F I S Q Y E Y L T K I T I Y M R G N G N F L R K V M ...................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 206 End: 394 ............................................................................................................................................................................................................. V Y I I N F L I F P L D S R P I Q P K S E K F I V P Y F F K N R S S K I K Q A N I I I L Y E H T K L P L H F Y K E L Q M .............................................................................................................................................................................................................................................................................................. taaagccacgtaataactgctgattagataatgcgaaacgtgcttgaatttatgcttacacttctaaataagtcttgataaatcaatatttattgatgttacaaggctttatttcttcagttttcatcacgtttaactagtaatagtgacctttgaataactctgtgttttcattaaatcgtagtgttcatttactttgtcactaatttcgcataacatatattatgttaaataaaataaatggtaagtcacttctgggaatttgaggtttgctctctttaaaaatgactgggtaaaaaaatttatttcttgaagatttgattttttgagcgtttatgatgatgagatattcgtgagtttttagaggtaggtgaaaatacttctctagctgaatctaggtttataaaaatcgactgatattcatagagggtttttatcgttatgtacatccttccgtttccattaaataatctcttaacaatttagttacaattgagaatttatttcagtaacactacttaaatgagaattgttgttattataaaattcattttaaattaaacacttactaagatattttatgagaatttgattgactattctcaataaggtgaataaaatgcgcacaacaaattaagttgtattgcgatctattaacaaattaaggaaaggagttggtt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 84 Start: 592 End: 621 ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ATTGACTATTCTCAATAAGGTGAATAAAAT........................................................... PromScan Matrix: RpoD-17 Strand: + Score: 82 Start: 190 End: 219 .............................................................................................................................................................................................ATTTACTTTGTCACTAATTTCGCATAACAT............................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaagccacgtaataactgctgattagataatgcgaaacgtgcttgaatttatgcttacacttctaaataagtcttgataaatcaatatttattgatgttacaaggctttatttcttcagttttcatcacgtttaactagtaatagtgacctttgaataactctgtgttttcattaaatcgtagtgttcatttactttgtcactaatttcgcataacatatattatgttaaataaaataaatggtaagtcacttctgggaatttgaggtttgctctctttaaaaatgactgggtaaaaaaatttatttcttgaagatttgattttttgagcgtttatgatgatgagatattcgtgagtttttagaggtaggtgaaaatacttctctagctgaatctaggtttataaaaatcgactgatattcatagagggtttttatcgttatgtacatccttccgtttccattaaataatctcttaacaatttagttacaattgagaatttatttcagtaacactacttaaatgagaattgttgttattataaaattcattttaaattaaacacttactaagatattttatgagaatttgattgactattctcaataaggtgaataaaatgcgcacaacaaattaagttgtattgcgatctattaacaaattaaggaaaggagttggtt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02119:AA02120|AA02119:AA02120:hypothetical protein:nonheme ferritin:->->:1446641..1447320 680 taaagccacgtaataactgctgattagataatgcgaaacgtgcttgaatttatgcttacacttctaaataagtcttgataaatcaatatttattgatgttacaaggctttatttcttcagttttcatcacgtttaactagtaatagtgacctttgaataactctgtgttttcattaaatcgtagtgttcatttactttgtcactaatttcgcataacatatattatgttaaataaaataaatggtaagtcacttctgggaatttgaggtttgctctctttaaaaatgactgggtaaaaaaatttatttcttgaagatttgattttttgagcgtttatgatgatgagatattcgtgagtttttagaggtaggtgaaaatacttctctagctgaatctaggtttataaaaatcgactgatattcatagagggtttttatcgttatgtacatccttccgtttccattaaataatctcttaacaatttagttacaattgagaatttatttcagtaacactacttaaatgagaattgttgttattataaaattcattttaaattaaacacttactaagatattttatgagaatttgattgactattctcaataaggtgaataaaatgcgcacaacaaattaagttgtattgcgatctattaacaaattaaggaaaggagttggtt Intra-Species Hit: Count: 1 Min: 1 Max: 680 Len: 680 Subject: aact_AA02119_AA02120|hypothetical protein:nonheme ferritin|POSITIVE:POSITIVE|[1446641,1447320]|680 HSP 1 e-value: 0.0 bit: 1306.0 Len: 680 Query Start:1 Query End:680 Subject Strand: POSITIVE Subject Start: 1 Subject End: 680 taaagccacgtaataactgctgattagataatgcgaaacgtgcttgaatttatgcttacacttctaaataagtcttgataaatcaatatttattgatgttacaaggctttatttcttcagttttcatcacgtttaactagtaatagtgacctttgaataactctgtgttttcattaaatcgtagtgttcatttactttgtcactaatttcgcataacatatattatgttaaataaaataaatggtaagtcacttctgggaatttgaggtttgctctctttaaaaatgactgggtnnnnnnntttatttcttgaagatttgattttttgagcgtttatgatgatgagatattcgtgagtttttagaggtaggtgaaaatacttctctagctgaatctaggtttataaaaatcgactgatattcatagagggtttttatcgttatgtacatccttccgtttccattaaataatctcttaacaatttagttacaattgagaatttatttcagtaacactacttaaatgagaattgttgttattataaaattcattttaaattaaacacttactaagatattttatgagaatttgattgactattctcaataaggtgaataaaatgcgcacaacaaattaagttgtattgcgatctattaacaaattaaggaaaggagttggtt taaagccacgtaataactgctgattagataatgcgaaacgtgcttgaatttatgcttacacttctaaataagtcttgataaatcaatatttattgatgttacaaggctttatttcttcagttttcatcacgtttaactagtaatagtgacctttgaataactctgtgttttcattaaatcgtagtgttcatttactttgtcactaatttcgcataacatatattatgttaaataaaataaatggtaagtcacttctgggaatttgaggtttgctctctttaaaaatgactgggtaaaaaaatttatttcttgaagatttgattttttgagcgtttatgatgatgagatattcgtgagtttttagaggtaggtgaaaatacttctctagctgaatctaggtttataaaaatcgactgatattcatagagggtttttatcgttatgtacatccttccgtttccattaaataatctcttaacaatttagttacaattgagaatttatttcagtaacactacttaaatgagaattgttgttattataaaattcattttaaattaaacacttactaagatattttatgagaatttgattgactattctcaataaggtgaataaaatgcgcacaacaaattaagttgtattgcgatctattaacaaattaaggaaaggagttggtt Inter-species Hit: Count: 1 Min: 133 Max: 239 Len: 107 Subject: NC_002663_PM0611_PM0612|SbcB:hypothetical protein|POSITIVE:POSITIVE|[713529,714397]|869 HSP 1 e-value: 5.0E-13 bit: 77.8 Len: 107 Query Start:133 Query End:239 Subject Strand: POSITIVE Subject Start: 236 Subject End: 342 ....................................................................................................................................ttaactagtaatagtgacctttgaataactctgtgttttcattaaatcgtagtgttcatttactttgtcactaatttcgcataacatatattatgttaaataaaata......................................................................................................................................................................................................................................................................................................................................................................................................................................................... ....................................................................................................................................ttaagtagtaaaagtgacctgtgtataagtctgtgatttatttatatcgtagtgtttattaactttgtaacagacttcgcataatgtatattatgttaaataaaata......................................................................................................................................................................................................................................................................................................................................................................................................................................................... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAACUAGUAAUAGUGACCUUUGAAUAACUCUGUGUUUUCAUUAAAUCGUAGUGUUCAUUUACUUUGUCACUAAUUUCGCAUAACAUAUAUUAUGUUAAAUAAAAUA ...........(((((((...((((((((.((((((((.....))).))))).))).)))))....)))))))........(((((((...)))))))......... (-16.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUUUUAUUUAACAUAAUAUAUGUUAUGCGAAAUUAGUGACAAAGUAAAUGAACACUACGAUUUAAUGAAAACACAGAGUUAUUCAAAGGUCACUAUUACUAGUUAA .(((((...(((((((...)))))))...)))))(((((((..(((........))).........(((((((.....))).))))...)))))))........... (-15.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 237 80 46 173 133 aact:AA02693|5end_hydrogenase-4_component_H_1877921..1878151_POSITIVE 237 58 11 53 2 aact:AA02681|5end_hypothetical_protein_1865749..1865979_POSITIVE 236 80 45 180 136 aact:AA00310|5end_conserved_hypothetical_protein_210193..210423_POSITIVE 224 80 31 223 179 aact:AA02521|5end_conserved_hypothetical_protein_1752603..1752833_POSITIVE 215 82 45 78 35 aact:AA01774|5end_5,10-methylenetetrahydrofolate_reductase_1195015..1195245_POSITIVE 208 90 47 138 88 aact:AA02090|5end_glucose-1-phosphate_adenylyltransferase_1423162..1423392_POSITIVE 205 80 32 205 152 aact:AA02978|5end_sufI_protein_precursor_homolog_2081813..2082043_POSITIVE 202 71 34 216 178 aact:AA00930|5end_pantothenate_kinase_640275..640505_POSITIVE 200 79 40 191 153 aact:AA02566|5end_PTS_system,_IIC_component_1787008..1787238_POSITIVE 200 80 38 96 45 aact:AA02109|5end_2-acylglycerophosphoethanolamine_acyltransferase;_acyl-acyl_carrier_protein_synthetase_1436563..1436793_POSITIVE 196 79 43 191 154 aact:AA02182|5end_tryptophan_biosynthesis_protein_trpCF_1491038..1491268_POSITIVE 196 80 31 100 51 aact:AA00637|5end_phosphohistidine_phosphatase_438169..438399_POSITIVE 194 57 13 143 92 aact:AA01256|5end_hypothetical_protein_852774..853004_POSITIVE 193 43 7 108 70 aact:AA00237|5end_hypothetical_protein_170934..171164_POSITIVE 191 57 11 215 162 aact:AA00908|5end_probable_sigma-E_factor_regulatory_protein_626076..626306_POSITIVE 191 57 11 109 56 aact:AA00909.1|5end_hypothetical_protein_625970..626200_POSITIVE 191 80 33 137 81 aact:AA00796|5end_iron(III)_dicitrate_transport_system_permease_protein_545686..545916_POSITIVE 191 78 32 173 131 aact:AA00018|5end_hypothetical_protein_10550..10780_POSITIVE 189 60 14 230 178 aact:AA02063|5end_23S_rRNA_psedouridine_synthase,_Rlu_family_protein_1404451..1404681_POSITIVE 189 84 47 208 156 aact:AA01450|5end_hypothetical_protein_971860..972090_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 215 82 45 95 52 aact:AA01773|3end_hypothetical_protein_1195112..1195262_POSITIVE 208 90 47 108 58 aact:AA02089|3end_glycogen_operon_protein_1423212..1423362_POSITIVE 200 80 38 96 45 aact:AA02108|3end_2-acylglycerophosphoethanolamine_acyltransferase;_acyl-acyl_carrier_protein_synthetase_1436643..1436793_POSITIVE 195 69 30 128 92 aact:AA01342|3end_conserved_hypotheticl_protein_(possible_ribosomal_RNA_adenine_dimethylase)_911085..911235_POSITIVE 193 82 48 146 104 aact:AA02000|3end_2,3,4,5-tetrahydropyridine-2-carboxylate_1359801..1359951_POSITIVE 193 43 7 115 77 aact:AA00236|3end_anaerobic_ribonucleoside-triphosphate_reductase_171021..171171_POSITIVE 192 66 30 40 4 aact:AA02679|3end_formate_dehydrogenase,_gamma_subunit_1865811..1865961_POSITIVE 191 46 7 148 111 aact:AA01255|3end_hypothetical_protein_852873..853023_POSITIVE 191 80 33 134 78 aact:AA00795|3end_iron(III)_dicitrate-binding_periplasmic_protein_precursor_545763..545913_POSITIVE 190 79 31 151 108 aact:AA02519|3end_DNA_gyrase_A_1752612..1752762_POSITIVE 189 60 14 111 59 aact:AA02062|3end_conserved_hypothetical_protein_(probable_membrane_protein)_1404412..1404562_POSITIVE 184 80 37 59 9 aact:AA02271|3end_hypothetical_protein_1548801..1548951_POSITIVE 183 85 46 121 80 aact:AA02368|3end_conserved_hypothetical_protein_1624286..1624436_POSITIVE 180 80 32 128 81 aact:AA00241|3end_formate_acetyltransferase_activating_enzyme_(anaerobic_ribonucleoside-triphosphate_reductase_activating_protein)_172090..172240_POSITIVE 179 80 48 93 59 aact:AA02030|3end_threonyl-tRNA_synthetase_1384179..1384329_POSITIVE 178 79 32 133 90 aact:AA01249|3end_conserved_hypothetical_protein_(possible_phosphoesterase)_850423..850573_POSITIVE 175 91 57 52 19 aact:AA01827|3end_iron(III)_ABC_transporter,_periplasmic_binding_protein_1231407..1231557_POSITIVE 174 78 46 37 2 aact:AA02328|3end_anti-sigma_factor_B_antagonist_1589736..1589886_POSITIVE 174 64 26 136 89 aact:AA02171|3end_polysialic_acid_capsule_expression_protein_1486096..1486246_POSITIVE 174 54 17 143 103 aact:AA01341|3end_acriflavine_resistance_protein_910131..910281_POSITIVE