Origin IGS: taattccgactgaaataaaatcgccttattgttgagcagtaaggcgatttttttatccctccaaagtgcggttgtaaaagataatgttttttaaccctgtcgaaagaaacctgctaaaatagcaacagttttagtttccctgaatgccct .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agggcattcagggaaactaaaactgttgctattttagcaggtttctttcgacagggttaaaaaacattatcttttacaaccgcactttggagggataaaaaaatcgccttactgctcaacaataaggcgattttatttcagtcggaatta Mask Tandem Repeat Region ================================================ taattccgactgaaataaaatcgccttattgttgagcagtaaggcgatttttttatccctccaaagtgcggttgtaaaagataatgttttttaaccctgtcgaaagaaacctgctaaaatagcaacagttttagtttccctgaatgccct Find is-nt database================================================ Query_seq: AA02100:AA02101|AA02100:AA02101:lipoprotein:penicillin-binding protein 7:->->:1430820..1430969 150 taattccgactgaaataaaatcgccttattgttgagcagtaaggcgatttttttatccctccaaagtgcggttgtaaaagataatgttttttaaccctgtcgaaagaaacctgctaaaatagcaacagttttagtttccctgaatgccct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02100:AA02101|AA02100:AA02101:lipoprotein:penicillin-binding protein 7:->->:1430820..1430969 150 taattccgactgaaataaaatcgccttattgttgagcagtaaggcgatttttttatccctccaaagtgcggttgtaaaagataatgttttttaaccctgtcgaaagaaacctgctaaaatagcaacagttttagtttccctgaatgccct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02100:AA02101|AA02100:AA02101:lipoprotein:penicillin-binding protein 7:->->:1430820..1430969 150 taattccgactgaaataaaatcgccttattgttgagcagtaaggcgatttttttatccctccaaagtgcggttgtaaaagataatgttttttaaccctgtcgaaagaaacctgctaaaatagcaacagttttagtttccctgaatgccct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taattccgactgaaataaaatcgccttattgttgagcagtaaggcgatttttttatccctccaaagtgcggttgtaaaagataatgttttttaaccctgtcgaaagaaacctgctaaaatagcaacagttttagtttccctgaatgccct Predict ORF larger than 30AA ================================================ taattccgactgaaataaaatcgccttattgttgagcagtaaggcgatttttttatccctccaaagtgcggttgtaaaagataatgttttttaaccctgtcgaaagaaacctgctaaaatagcaacagttttagtttccctgaatgccct Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taattccgactgaaataaaatcgccttattgttgagcagtaaggcgatttttttatccctccaaagtgcggttgtaaaagataatgttttttaaccctgtcgaaagaaacctgctaaaatagcaacagttttagtttccctgaatgccct Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -9.5 Tail_Score: -4.90778 Start: 21 End: 47 Full_Region: ggagggataaaaaaa tcgccttactg ctcaa caataaggcga ttttatttcagtcgg ....................tcgccttactgctcaacaataaggcga....................................................................................................... Find igs database================================================ Query_seq: AA02100:AA02101|AA02100:AA02101:lipoprotein:penicillin-binding protein 7:->->:1430820..1430969 150 taattccgactgaaataaaatcgccttattgttgagcagtaaggcgatttttttatccctccaaagtgcggttgtaaaagataatgttttttaaccctgtcgaaagaaacctgctaaaatagcaacagttttagtttccctgaatgccct Intra-Species Hit: Count: 1 Min: 1 Max: 150 Len: 150 Subject: aact_AA02100_AA02101|lipoprotein:penicillin-binding protein 7|POSITIVE:POSITIVE|[1430820,1430969]|150 HSP 1 e-value: 2.0E-67 bit: 256.0 Len: 150 Query Start:1 Query End:150 Subject Strand: POSITIVE Subject Start: 1 Subject End: 150 taattccgactgaaataaaatcgccttattgttgagcagtaaggcgannnnnnnatccctccaaagtgcggttgtaaaagataatgttttttaaccctgtcgaaagaaacctgctaaaatagcaacagttttagtttccctgaatgccct taattccgactgaaataaaatcgccttattgttgagcagtaaggcgatttttttatccctccaaagtgcggttgtaaaagataatgttttttaaccctgtcgaaagaaacctgctaaaatagcaacagttttagtttccctgaatgccct Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUCCGACUGAAAUAAAAUCGCCUUAUUGUUGAGCAGUAAGGCGAUUUUUUUAUCCCUCCAAAGUGCGGUUGUAAAAGAUAAUGUUUUUUAACCCUGUCGAAAGAAACCUGCUAAAAUAGCAACAGUUUUAGUUUCCCUGAAUGCCCU ..((((.((((((((((((((((((((((((.....)))))))))))))))..................((((......((((..(((....)))..))))......)))).((((...))))....))))))))).....))))..... (-36.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGGGCAUUCAGGGAAACUAAAACUGUUGCUAUUUUAGCAGGUUUCUUUCGACAGGGUUAAAAAACAUUAUCUUUUACAACCGCACUUUGGAGGGAUAAAAAAAUCGCCUUACUGCUCAACAAUAAGGCGAUUUUAUUUCAGUCGGAAUUA ..........((....))....((((((......)))))).....(((((((..............(((((((((.(((.......)))))))))))).((((((((((((.((.....)).))))))))))))......)))))))... (-37.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 381 96 51 187 134 aact:AA02902|5end_aerobic_respiration_control_protein_2031171..2031401_POSITIVE 368 103 62 174 125 aact:AA01090|5end_leucyl-tRNA_synthetase,_leucyl-tRNA_ligase_753548..753778_POSITIVE 355 95 63 152 120 aact:AA02666|5end_DNA_helicase_II_1854410..1854640_POSITIVE 351 107 63 164 112 aact:AA01069|5end_translation_elongation_factor_(EF-Ts)_739775..740005_POSITIVE 351 107 63 58 6 aact:AA01068|5end_hypothetical_protein_739669..739899_POSITIVE 329 99 63 183 144 aact:AA02380|5end_iron_chelatin_ABC_transporter,periplasmic-binding_protein_1628057..1628287_POSITIVE 329 99 63 80 41 aact:AA02379|5end_hypothetical_protein_1627954..1628184_POSITIVE 320 110 63 168 121 aact:AA02885|5end_conserved_hypothetical_protein_2022428..2022658_POSITIVE 317 109 63 172 114 aact:AA00766|5end_heat_shock_protein_B25.3_(HSP-70_cofactor)_527384..527614_POSITIVE 314 98 58 190 144 aact:AA01173|5end_VapD-homolog_803850..804080_POSITIVE 312 91 61 213 180 aact:AA02932|5end_lytic_murein_transglycosylase_A_2052349..2052579_POSITIVE 308 110 63 189 145 aact:AA00569|5end_ketol-acid_reductoisomerase_391191..391421_POSITIVE 305 95 63 231 198 aact:AA02168|5end_possible_protease;_heat_shock_protein_1483529..1483759_POSITIVE 303 50 4 135 80 aact:AA02234|5end_hypothetical_protein_1521341..1521571_POSITIVE 299 98 59 174 132 aact:AA00591|5end_ABC_transporter,_ATP-binding_protein_403613..403843_POSITIVE 297 72 37 214 177 aact:AA01592|5end_hypothetical_protein_1070950..1071180_POSITIVE 296 93 53 123 83 aact:AA02306|5end_DNA-directed_RNA_polymerase,_omega_chain_1576947..1577177_POSITIVE 295 103 63 194 148 aact:AA01965|5end_poly(A)_polymerase_1329478..1329708_POSITIVE 293 70 26 112 72 aact:AA02051|5end_geranylgeranyl_pyrophosphate_synthase;_farnesyl-diphosphate_synthase_1397560..1397790_POSITIVE 291 100 63 135 98 aact:AA01776|5end_conserved_hypothetical_protein_1196074..1196304_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 443 50 6 73 20 aact:AA02100|3end_lipoprotein_1430759..1430909_POSITIVE 381 96 51 74 21 aact:AA02899|3end_oligopeptide_ABC_transporter,_ATP-binding_protein_2031138..2031288_POSITIVE 368 103 62 143 94 aact:AA01089|3end_hypothetical_protein_753597..753747_POSITIVE 347 96 58 140 95 aact:AA01068|3end_hypothetical_protein_739850..740000_POSITIVE 320 110 63 87 40 aact:AA02883|3end_conserved_hypothetical_protein_2022427..2022577_POSITIVE 314 98 58 107 61 aact:AA01172|3end_conserved_hypothetical_protein_803847..803997_POSITIVE 305 95 63 96 63 aact:AA02167|3end_hypothetical_protein_1483474..1483624_POSITIVE 303 50 4 71 16 aact:AA02233|3end_asparaginyl-tRNA_synthetase_1521357..1521507_POSITIVE 297 72 37 55 18 aact:AA01589|3end_conserved_hypothetical_protein_1070871..1071021_POSITIVE 296 93 53 74 34 aact:AA02305|3end_guanosine-3',5'-bis(diphosphate)3'-_pyrophosphohydrolase_1576978..1577128_POSITIVE 295 103 63 74 28 aact:AA01963|3end_dnaK_suppressor_protein_1329438..1329588_POSITIVE 289 47 6 55 9 aact:AA01080|3end_hypothetical_protein_747884..748034_POSITIVE 282 56 20 141 98 aact:AA00347|3end_50S_ribosomal_protein_L11_232642..232792_POSITIVE 276 103 61 55 15 aact:AA02050|3end_1-deoxy-D-xylulose_5-phosphate_synthase_1397552..1397702_POSITIVE 275 47 18 52 19 aact:AA00525|3end_hypothetical_protein_359459..359609_POSITIVE 271 93 63 87 57 aact:AA02023|3end_NADH_dehydrogenase_1378115..1378265_POSITIVE 271 79 39 148 104 aact:AA01543|3end_hypothetical_protein_1034350..1034500_POSITIVE 269 57 19 81 40 aact:AA02300|3end_glutamate_racemase_1572252..1572402_POSITIVE 269 98 59 58 17 aact:AA02048|3end_galactitol_utilization_operon_repressor_1395579..1395729_POSITIVE 266 102 63 106 51 aact:AA00166|3end_conserved_hypothetical_protein_(possible_acetyltransferase)_117919..118069_POSITIVE