Origin IGS: taatgattgataaaactccggttattgccggagttttttctttaggcggaaataaggttaggaacaaataatttaattttctgtctaaggcccatgttttgtccgtttatcggatcaatacttatccataaactcataaaggattttaat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 attaaaatcctttatgagtttatggataagtattgatccgataaacggacaaaacatgggccttagacagaaaattaaattatttgttcctaaccttatttccgcctaaagaaaaaactccggcaataaccggagttttatcaatcatta Mask Tandem Repeat Region ================================================ taatgattgataaaactccggttattgccggagttttttctttaggcggaaataaggttaggaacaaataatttaattttctgtctaaggcccatgttttgtccgtttatcggatcaatacttatccataaactcataaaggattttaat Find is-nt database================================================ Query_seq: AA02099:AA02100|AA02099:AA02100:50S ribosomal protein L25:lipoprotein:->->:1430064..1430213 150 taatgattgataaaactccggttattgccggagttttttctttaggcggaaataaggttaggaacaaataatttaattttctgtctaaggcccatgttttgtccgtttatcggatcaatacttatccataaactcataaaggattttaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02099:AA02100|AA02099:AA02100:50S ribosomal protein L25:lipoprotein:->->:1430064..1430213 150 taatgattgataaaactccggttattgccggagttttttctttaggcggaaataaggttaggaacaaataatttaattttctgtctaaggcccatgttttgtccgtttatcggatcaatacttatccataaactcataaaggattttaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02099:AA02100|AA02099:AA02100:50S ribosomal protein L25:lipoprotein:->->:1430064..1430213 150 taatgattgataaaactccggttattgccggagttttttctttaggcggaaataaggttaggaacaaataatttaattttctgtctaaggcccatgttttgtccgtttatcggatcaatacttatccataaactcataaaggattttaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatgattgataaaactccggttattgccggagttttttctttaggcggaaataaggttaggaacaaataatttaattttctgtctaaggcccatgttttgtccgtttatcggatcaatacttatccataaactcataaaggattttaat Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 52 End: 150 ................................................... M R L G T N N L I F C L R P M F C P F I G S I L I H K L I K D F N Protein_Len: 31 Strand: - Start: 45 End: 137 ............................................ A S I L N P V F L K I K Q R L G M N Q G N I P D I S I W L S M ............. taatgattgataaaactccggttattgccggagttttttctttaggcggaaataaggttaggaacaaataatttaattttctgtctaaggcccatgttttgtccgtttatcggatcaatacttatccataaactcataaaggattttaat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatgattgataaaactccggttattgccggagttttttctttaggcggaaataaggttaggaacaaataatttaattttctgtctaaggcccatgttttgtccgtttatcggatcaatacttatccataaactcataaaggattttaat Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 47 HP_score: -2.6 Tail_Score: -3.36327 Start: 96 End: 121 Full_Region: CTGTCTAAGGCCCAT GTTTTGTCCG TTTAT CGGATCAATAC TTATCCATAAACTCA ...............................................................................................GTTTTGTCCGTTTATCGGATCAATAC............................. Find igs database================================================ Query_seq: AA02099:AA02100|AA02099:AA02100:50S ribosomal protein L25:lipoprotein:->->:1430064..1430213 150 taatgattgataaaactccggttattgccggagttttttctttaggcggaaataaggttaggaacaaataatttaattttctgtctaaggcccatgttttgtccgtttatcggatcaatacttatccataaactcataaaggattttaat Intra-Species Hit: Count: 1 Min: 1 Max: 150 Len: 150 Subject: aact_AA02099_AA02100|50S ribosomal protein L25:lipoprotein|POSITIVE:POSITIVE|[1430064,1430213]|150 HSP 1 e-value: 5.0E-80 bit: 297.0 Len: 150 Query Start:1 Query End:150 Subject Strand: POSITIVE Subject Start: 1 Subject End: 150 taatgattgataaaactccggttattgccggagttttttctttaggcggaaataaggttaggaacaaataatttaattttctgtctaaggcccatgttttgtccgtttatcggatcaatacttatccataaactcataaaggattttaat taatgattgataaaactccggttattgccggagttttttctttaggcggaaataaggttaggaacaaataatttaattttctgtctaaggcccatgttttgtccgtttatcggatcaatacttatccataaactcataaaggattttaat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUGAUUGAUAAAACUCCGGUUAUUGCCGGAGUUUUUUCUUUAGGCGGAAAUAAGGUUAGGAACAAAUAAUUUAAUUUUCUGUCUAAGGCCCAUGUUUUGUCCGUUUAUCGGAUCAAUACUUAUCCAUAAACUCAUAAAGGAUUUUAAU ...........(((((((((((....)))))))))))..(((((((((((((..((((((........))))))...)))))))))))))...((.((((((..((((((.((((........))))))))))..)))))).))...... (-42.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUAAAAUCCUUUAUGAGUUUAUGGAUAAGUAUUGAUCCGAUAAACGGACAAAACAUGGGCCUUAGACAGAAAAUUAAAUUAUUUGUUCCUAACCUUAUUUCCGCCUAAAGAAAAAACUCCGGCAAUAACCGGAGUUUUAUCAAUCAUUA .............((((......(((...((.(((.((((.....))))))).)).((((.....((((((.(((...))).))))))))))........))).......((.((((((((((......)))))))))).))..)))).. (-32.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 315 47 3 200 158 aact:AA01624|5end_glucose-6-phosphate_isomerase_1098175..1098405_POSITIVE 311 58 14 66 15 aact:AA01182|5end_3-oxoacyl-(acyl-carrier-protein)_synthase_809210..809440_POSITIVE 302 117 81 57 24 aact:AA00250|5end_lipoate_biosynthesis_protein_A_175496..175726_POSITIVE 297 50 4 229 193 aact:AA00041|5end_cytochrome_c-type_biogenesis_protein_31881..32111_POSITIVE 290 58 16 88 43 aact:AA02500|5end_conserved_hypothetical_protein_1734611..1734841_POSITIVE 289 58 12 158 109 aact:AA01385|5end_50S_ribosomal_protein_L9_935509..935739_POSITIVE 287 119 82 83 36 aact:AA02964|5end_opacity_associated_protein_A_2072738..2072968_POSITIVE 286 50 7 138 92 aact:AA02906|5end_hypothetical_protein_2033304..2033534_POSITIVE 284 58 16 61 22 aact:AA02966|5end_conserved_hypothetical_protein_2074223..2074453_POSITIVE 281 58 18 157 115 aact:AA02926|5end_hypothetical_protein_2048136..2048366_POSITIVE 280 59 16 58 1 aact:AA02316|5end_exodeoxyribonuclease_VII_large_subunit_1581682..1581912_POSITIVE 280 63 21 167 118 aact:AA00725|5end_translation_initiation_factor_1_497981..498211_POSITIVE 280 57 18 185 143 aact:AA00056|5end_cytochrome_c-type_biogenesis_protein_43925..44155_POSITIVE 277 59 15 173 126 aact:AA02886|5end_OBG-related_GTP-binding_protein_2023382..2023612_POSITIVE 276 50 19 64 19 aact:AA02724|5end_seryl-tRNA_ligase_1906357..1906587_POSITIVE 274 59 17 208 155 aact:AA00373|5end_CoA-transferase_(beta)_251013..251243_POSITIVE 270 51 15 53 17 aact:AA01022|5end_tRNA_(5-methylaminomethyl-2-thiouridylate)_methyltransferase_714689..714919_POSITIVE 266 58 15 182 123 aact:AA00204|5end_(di)nucleoside_polyphosphate_hydrolase;_possible_invasion_protein_141346..141576_POSITIVE 263 128 81 168 119 aact:AA03009|5end_hypothetical_protein_2101001..2101231_POSITIVE 262 128 81 94 30 aact:AA01066|5end_conserved_hypothetical_protein_738380..738610_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 311 58 14 70 19 aact:AA01181|3end_conserved_hypothetical_protein_809294..809444_POSITIVE 289 58 12 109 60 aact:AA01384|3end_hypothetical_protein_935540..935690_POSITIVE 284 58 16 44 5 aact:AA02965|3end_hypothetical_protein_2074286..2074436_POSITIVE 281 58 18 92 50 aact:AA02925|3end_conserved_hypothetical_protein_2048151..2048301_POSITIVE 280 63 21 142 93 aact:AA00724|3end_hypothetical_protein_498036..498186_POSITIVE 277 59 15 133 86 aact:AA02885|3end_conserved_hypothetical_protein_2023422..2023572_POSITIVE 276 50 19 80 35 aact:AA02723|3end_hypothetical_protein_1906453..1906603_POSITIVE 262 116 80 94 52 aact:AA00377|3end_acetyl-CoA_acetyltransferase_(Beta-ketothiolase,3-ketoacyl-CoA_thiolase)_254300..254450_POSITIVE 261 47 2 144 96 aact:AA01816|3end_hypothetical_protein_1224134..1224284_POSITIVE 259 50 19 137 102 aact:AA01900|3end_hypothetical_protein_1282582..1282732_POSITIVE 257 60 19 146 102 aact:AA02002|3end_hypothetical_protein_1360407..1360557_POSITIVE 256 37 12 79 54 aact:AA02099|3end_50S_ribosomal_protein_L25_1430003..1430153_POSITIVE 256 49 15 149 117 aact:AA00202|3end_conserved_hypothetical_protein_(possible_membrane_protein)_141420..141570_POSITIVE 252 127 85 135 82 aact:AA02544|3end_ATP-dependent_RNA_helicase_1773945..1774095_POSITIVE 247 52 18 83 33 aact:AA02501|3end_DNA_polymerase_III,_subunits_gamma_and_tau_1737626..1737776_POSITIVE 247 57 16 64 16 aact:AA01336|3end_hypothetical_protein_904315..904465_POSITIVE 247 57 17 74 23 aact:AA00248|3end_hypothetical_protein_174049..174199_POSITIVE 247 48 20 116 86 aact:AA00116|3end_conserved_hypothetical_protein_88207..88357_POSITIVE 245 116 78 135 99 aact:AA01843|3end_sensor_protein;_probable_two-component_system_sensor_histidine_kinase_1239830..1239980_POSITIVE 243 49 9 86 43 aact:AA01304|3end_penicillin-binding_protein_1A_885580..885730_POSITIVE