Origin IGS: taatgaaatagttaatctttcgttcaagtaaaaatacggcttgaacggaagattattattgtttttttatctcgaatgattaaaatttcaccttttttttgattgttaactgaggactattttat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ataaaatagtcctcagttaacaatcaaaaaaaaggtgaaattttaatcattcgagataaaaaaacaataataatcttccgttcaagccgtatttttacttgaacgaaagattaactatttcatta Mask Tandem Repeat Region ================================================ taatgaaatagttaatctttcgttcaagtaaaaatacggcttgaacggaagattattattgtttttttatctcgaatgattaaaatttcaccttttttttgattgttaactgaggactattttat Find is-nt database================================================ Query_seq: AA02092:AA02094|AA02092:AA02094:glycogen synthase:glycogen phosphorylase; glycogen synthase:->->:1426251..1426375 125 taatgaaatagttaatctttcgttcaagtaaaaatacggcttgaacggaagattattattgtttttttatctcgaatgattaaaatttcaccttttttttgattgttaactgaggactattttat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02092:AA02094|AA02092:AA02094:glycogen synthase:glycogen phosphorylase; glycogen synthase:->->:1426251..1426375 125 taatgaaatagttaatctttcgttcaagtaaaaatacggcttgaacggaagattattattgtttttttatctcgaatgattaaaatttcaccttttttttgattgttaactgaggactattttat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02092:AA02094|AA02092:AA02094:glycogen synthase:glycogen phosphorylase; glycogen synthase:->->:1426251..1426375 125 taatgaaatagttaatctttcgttcaagtaaaaatacggcttgaacggaagattattattgtttttttatctcgaatgattaaaatttcaccttttttttgattgttaactgaggactattttat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatgaaatagttaatctttcgttcaagtaaaaatacggcttgaacggaagattattattgtttttttatctcgaatgattaaaatttcaccttttttttgattgttaactgaggactattttat Predict ORF larger than 30AA ================================================ taatgaaatagttaatctttcgttcaagtaaaaatacggcttgaacggaagattattattgtttttttatctcgaatgattaaaatttcaccttttttttgattgttaactgaggactattttat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatgaaatagttaatctttcgttcaagtaaaaatacggcttgaacggaagattattattgtttttttatctcgaatgattaaaatttcaccttttttttgattgttaactgaggactattttat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02092:AA02094|AA02092:AA02094:glycogen synthase:glycogen phosphorylase; glycogen synthase:->->:1426251..1426375 125 taatgaaatagttaatctttcgttcaagtaaaaatacggcttgaacggaagattattattgtttttttatctcgaatgattaaaatttcaccttttttttgattgttaactgaggactattttat Intra-Species Hit: Count: 1 Min: 1 Max: 125 Len: 125 Subject: aact_AA02092_AA02094|glycogen synthase:glycogen phosphorylase; glycogen synthase|POSITIVE:POSITIVE|[1426251,1426375]|125 HSP 1 e-value: 3.0E-38 bit: 159.0 Len: 125 Query Start:1 Query End:125 Subject Strand: POSITIVE Subject Start: 1 Subject End: 125 taatgaaatagttaatctttcgttcaagtaaaaatacggcttgaacggaagattattattgnnnnnnnatctcgaatgattaaaatttcaccnnnnnnnngattgttaactgaggactattttat taatgaaatagttaatctttcgttcaagtaaaaatacggcttgaacggaagattattattgtttttttatctcgaatgattaaaatttcaccttttttttgattgttaactgaggactattttat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUGAAAUAGUUAAUCUUUCGUUCAAGUAAAAAUACGGCUUGAACGGAAGAUUAUUAUUGUUUUUUUAUCUCGAAUGAUUAAAAUUUCACCUUUUUUUUGAUUGUUAACUGAGGACUAUUUUAU ..(((((((((((((((((((((((((((.........)))))))))))))))))...............((((((..(((((((...........)))))))..))...)))).)))))))))) (-35.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAAAAUAGUCCUCAGUUAACAAUCAAAAAAAAGGUGAAAUUUUAAUCAUUCGAGAUAAAAAAACAAUAAUAAUCUUCCGUUCAAGCCGUAUUUUUACUUGAACGAAAGAUUAACUAUUUCAUUA .................................((((((((....(((......))).............(((((((.((((((((...........)))))))).)))))))...)))))))). (-20.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 264 50 3 185 138 aact:AA01799|5end_polysacharide_biosynthesis_protein_(Orf14_of_cluster)_1212459..1212689_POSITIVE 246 55 11 57 21 aact:AA02288|5end_conserved_hypothetical_protein_1558590..1558820_POSITIVE 237 70 22 70 15 aact:AA01086|5end_hypothetical_protein_751392..751622_POSITIVE 233 74 37 41 1 aact:AA01650|5end_maltose_transport_inner_membrane_protein_1118228..1118458_POSITIVE 220 48 20 39 13 aact:AA01626|5end_N_utilization_substance_protein_B_1100444..1100674_POSITIVE 215 54 17 191 150 aact:AA01801|5end_malate_dehydrogenase_1213358..1213588_POSITIVE 215 54 17 44 3 aact:AA01800|5end_hypothetical_protein_1213211..1213441_POSITIVE 209 80 31 84 27 aact:AA02780|5end_protein-export_membrane_protein_1943072..1943302_POSITIVE 203 119 73 120 71 aact:AA02294|5end_conserved_hypothetical_protein_1563758..1563988_POSITIVE 202 49 10 169 124 aact:AA02745|5end_isopentenyl_monophosphate_kinase_1923279..1923509_POSITIVE 199 79 37 162 117 aact:AA00959|5end_nitrate/nitrite_response_regulator_664596..664826_POSITIVE 196 49 4 222 174 aact:AA00852|5end_hypothetical_protein_588538..588768_POSITIVE 192 47 20 81 58 aact:AA00566|5end_30S_ribosomal_protein_S12_(streptomycin_resistance_protein)_390595..390825_POSITIVE 190 53 11 60 27 aact:AA01138|5end_anaerobic_dimethyl_sulfoxide_reductase,_chain_B_780991..781221_POSITIVE 188 45 18 115 82 aact:AA02324|5end_ABC_transporter_ATP_binding_protein_1586518..1586748_POSITIVE 186 72 31 187 142 aact:AA02100|5end_lipoprotein_1430074..1430304_POSITIVE 186 80 36 188 138 aact:AA01503|5end_glycerol-3-phosphate_dehydrogenase_1010814..1011044_POSITIVE 185 94 56 104 64 aact:AA00987|5end_tetracenomycin_polyketide_synthesis_O-methyltransferase_689745..689975_POSITIVE 185 110 61 207 152 aact:AA00020|5end_conserved_hypothetical_protein_12799..13029_POSITIVE 183 70 34 108 60 aact:AA01930|5end_ribosome-binding_factor_A_1304712..1304942_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 353 60 13 84 36 aact:AA02092|3end_glycogen_synthase_1426190..1426340_POSITIVE 251 53 33 145 127 aact:AA00066|3end_NADP-dependent_malic_enzyme_(NADP-ME)_51343..51493_POSITIVE 222 70 27 49 1 aact:AA01085|3end_mutator_mutT_protein_(7,8-dihydro-8-oxoguanine-triphosphatase)_(8-oxo-dGTPase)_(dGTP_pyrophosphohydrolase)_751451..751601_POSITIVE 220 48 20 29 3 aact:AA01625|3end_riboflavin_synthase,_beta_chain_1100514..1100664_POSITIVE 202 48 1 115 72 aact:AA02566|3end_PTS_system,_IIC_component_1788179..1788329_POSITIVE 199 79 37 149 104 aact:AA00958|3end_adenylate_cyclase_664663..664813_POSITIVE 190 53 11 46 13 aact:AA01137|3end_anaerobic_dimethyl_sulfoxide_reductase,_chain_A_781057..781207_POSITIVE 185 110 61 150 95 aact:AA00019|3end_conserved_hypothetical_protein_12822..12972_POSITIVE 183 47 8 131 90 aact:AA01344|3end_magnesium_and_cobalt_transport_protein_912685..912835_POSITIVE 182 47 1 117 75 aact:AA00936|3end_ribose_ABC_transporter,_permease_protein,_C-terminal_646887..647037_POSITIVE 182 55 21 121 81 aact:AA00886|3end_hypothetical_protein_616855..617005_POSITIVE 181 48 14 145 114 aact:AA01983|3end_maltose_regulon_positive_regulatory_protein_1344738..1344888_POSITIVE 180 49 16 128 92 aact:AA00355|3end_DNA-directed_RNA_polymerase_beta_chain_(transcriptase_beta_chain)_238911..239061_POSITIVE 179 78 36 151 93 aact:AA01720|3end_conserved_hypothetical_protein_1163483..1163633_POSITIVE 178 51 17 136 94 aact:AA01575|3end_conserved_hypothetical_protein_1063147..1063297_POSITIVE 176 50 21 142 108 aact:AA01374|3end_tRNA/rRNA_methyltransferase_931097..931247_POSITIVE 176 52 12 149 109 aact:AA00030|3end_hypothetical_protein_25168..25318_POSITIVE 175 52 21 122 90 aact:AA02959|3end_pyrroline-5-carboxylate_(P5C)_reductase_2070976..2071126_POSITIVE 175 120 73 135 89 aact:AA00300|3end_conserved_hypothetical_protein_204353..204503_POSITIVE 173 79 37 47 2 aact:AA02780|3end_protein-export_membrane_protein_1944999..1945149_POSITIVE