Origin IGS: tgagatcactaatttgcactaaaagtgttgtttttgtgttaacctctactacaatacttaataattgttatttttcttgtcgtatgttgttgtaggttttat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ataaaacctacaacaacatacgacaagaaaaataacaattattaagtattgtagtagaggttaacacaaaaacaacacttttagtgcaaattagtgatctca Mask Tandem Repeat Region ================================================ tgagatcactaatttgcactaaaagtgttgtttttgtgttaacctctactacaatacttaataattgttatttttcttgtcgtatgttgttgtaggttttat Find is-nt database================================================ Query_seq: AA02084:AA02086|AA02084:AA02086:conserved hypothetical protein:4-alpha-glucanotransferase:->->:1418415..1418516 102 tgagatcactaatttgcactaaaagtgttgtttttgtgttaacctctactacaatacttaataattgttatttttcttgtcgtatgttgttgtaggttttat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02084:AA02086|AA02084:AA02086:conserved hypothetical protein:4-alpha-glucanotransferase:->->:1418415..1418516 102 tgagatcactaatttgcactaaaagtgttgtttttgtgttaacctctactacaatacttaataattgttatttttcttgtcgtatgttgttgtaggttttat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02084:AA02086|AA02084:AA02086:conserved hypothetical protein:4-alpha-glucanotransferase:->->:1418415..1418516 102 tgagatcactaatttgcactaaaagtgttgtttttgtgttaacctctactacaatacttaataattgttatttttcttgtcgtatgttgttgtaggttttat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgagatcactaatttgcactaaaagtgttgtttttgtgttaacctctactacaatacttaataattgttatttttcttgtcgtatgttgttgtaggttttat Predict ORF larger than 30AA ================================================ tgagatcactaatttgcactaaaagtgttgtttttgtgttaacctctactacaatacttaataattgttatttttcttgtcgtatgttgttgtaggttttat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgagatcactaatttgcactaaaagtgttgtttttgtgttaacctctactacaatacttaataattgttatttttcttgtcgtatgttgttgtaggttttat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02084:AA02086|AA02084:AA02086:conserved hypothetical protein:4-alpha-glucanotransferase:->->:1418415..1418516 102 tgagatcactaatttgcactaaaagtgttgtttttgtgttaacctctactacaatacttaataattgttatttttcttgtcgtatgttgttgtaggttttat Intra-Species Hit: Count: 1 Min: 1 Max: 102 Len: 102 Subject: aact_AA02084_AA02086|conserved hypothetical protein:4-alpha- glucanotransferase|POSITIVE:POSITIVE|[1418415, 1418516]|102 HSP 1 e-value: 2.0E-51 bit: 202.0 Len: 102 Query Start:1 Query End:102 Subject Strand: POSITIVE Subject Start: 1 Subject End: 102 tgagatcactaatttgcactaaaagtgttgtttttgtgttaacctctactacaatacttaataattgttatttttcttgtcgtatgttgttgtaggttttat tgagatcactaatttgcactaaaagtgttgtttttgtgttaacctctactacaatacttaataattgttatttttcttgtcgtatgttgttgtaggttttat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAGAUCACUAAUUUGCACUAAAAGUGUUGUUUUUGUGUUAACCUCUACUACAAUACUUAAUAAUUGUUAUUUUUCUUGUCGUAUGUUGUUGUAGGUUUUAU ......(((.(((..((((.....)))).)))...)))......(((((.((((((..(.((((..(......)..)))).)..)))))).)))))...... (-13.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAAAACCUACAACAACAUACGACAAGAAAAAUAACAAUUAUUAAGUAUUGUAGUAGAGGUUAACACAAAAACAACACUUUUAGUGCAAAUUAGUGAUCUCA .....................................((((((((...(((((.(((((((...............))))))).))))).)))))))).... (-12.66) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 239 46 27 226 207 aact:AA00530|5end_hypothetical_protein_362373..362603_POSITIVE 233 48 1 149 102 aact:AA01869|5end_periplasmic_serine_protease_do;_hhoA-like_precursor;_heat_shock_protein_1256650..1256880_POSITIVE 231 58 11 136 81 aact:AA02097|5end_DedA-family_integral_membrane_protein_1428823..1429053_POSITIVE 231 58 11 67 12 aact:AA02096|5end_hypothetical_protein_1428754..1428984_POSITIVE 229 46 1 133 92 aact:AA02708|5end_probable_glyoxalase_II_family_protein_1889926..1890156_POSITIVE 227 100 51 207 139 aact:AA00091|5end_short-patch_reverse_transcription_protein_67741..67971_POSITIVE 222 50 3 224 163 aact:AA01765|5end_Mu-like_prophage_FluMu_transposase_A_1191756..1191986_POSITIVE 219 50 3 43 2 aact:AA01813|5end_electron_transport_complex_protein_1220949..1221179_POSITIVE 217 98 65 53 15 aact:AA02861|5end_conserved_hypothetical_protein_2007721..2007951_POSITIVE 212 97 78 88 70 aact:AA00366|5end_50S_ribosomal_protein_L2_246215..246445_POSITIVE 208 51 19 42 11 aact:AA00606|5end_membrane-associated_zinc_metalloprotease_411808..412038_POSITIVE 202 94 71 203 181 aact:AA00837|5end_cell_division_protein_W_576317..576547_POSITIVE 201 100 77 39 17 aact:AA02420|5end_ISRSO11_related_transposase_1662070..1662300_POSITIVE 201 39 1 87 44 aact:AA02075|5end_conserved_hypothetical_protein_1409654..1409884_POSITIVE 199 44 1 165 124 aact:AA02119|5end_hypothetical_protein_1446372..1446602_POSITIVE 197 100 81 230 211 aact:AA02440|5end_ABC_transporter,_ATP-binding_protein_1685043..1685273_POSITIVE 197 49 5 137 86 aact:AA02218|5end_deoxyribose-phosphate_aldolase_(fragment?)_1509758..1509988_POSITIVE 197 53 13 197 162 aact:AA01127|5end_energy_transducer_protein_772685..772915_POSITIVE 197 47 1 133 87 aact:AA00835|5end_UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapep_tide)_575212..575442_POSITIVE 195 86 51 187 152 aact:AA02699|5end_formate_dehydrogenase_H,_selenopolypeptide_subunit_1881397..1881627_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 239 46 27 143 124 aact:AA00529|3end_outer_membrane_protein,_Omp_18/16_362370..362520_POSITIVE 231 48 3 45 1 aact:AA01868|3end_biotin_synthetase_1256626..1256776_POSITIVE 229 46 1 58 17 aact:AA02707|3end_hypothetical_protein_1889931..1890081_POSITIVE 227 100 51 135 67 aact:AA00089|3end_S-adenosylmethionine_synthase;_methionine_adenosyltransferase_67749..67899_POSITIVE 222 50 3 86 25 aact:AA01764|3end_hypothetical_protein_1191698..1191848_POSITIVE 220 45 2 69 29 aact:AA00030|3end_hypothetical_protein_25168..25318_POSITIVE 219 50 3 47 6 aact:AA01811|3end_electron_transport_complex_protein_1221033..1221183_POSITIVE 212 97 78 59 41 aact:AA00365|3end_30S_ribosomal_protein_S19_246266..246416_POSITIVE 203 88 43 144 102 aact:AA00977|3end_transcriptional_regulator_(LysR_family)_683334..683484_POSITIVE 202 100 75 98 72 aact:AA00761|3end_conserved_hypothetical_protein_(possible_peptidase)_524086..524236_POSITIVE 197 49 5 134 83 aact:AA02217|3end_deoxyribose-phosphate_aldolase_1509835..1509985_POSITIVE 197 47 1 59 13 aact:AA00833|3end_UDP-N-acetylmuramate-alanine_ligase_575218..575368_POSITIVE 195 86 51 135 100 aact:AA02698|3end_formate_dehydrogenase_H,_selenopolypeptide_subunit_1881425..1881575_POSITIVE 195 99 66 134 101 aact:AA00952|3end_hemY_protein_homolog_658895..659045_POSITIVE 193 38 14 79 50 aact:AA01080|3end_hypothetical_protein_747884..748034_POSITIVE 192 99 65 149 110 aact:AA00950|3end_conserved_hypothetical_protein_657574..657724_POSITIVE 186 49 1 144 96 aact:AA01874|3end_conserved_hypothetical_protein_1262451..1262601_POSITIVE 182 59 13 59 8 aact:AA02044|3end_phosphotransferase_system_enzyme_II,_galactitol_specific,_protein_A_1392014..1392164_POSITIVE 179 100 51 102 55 aact:AA00757|3end_aerobic_respiration_sensor-response_protein_520539..520689_POSITIVE 178 49 1 96 46 aact:AA00974|3end_glucosamine-fructose-6-phosphate_681127..681277_POSITIVE