Origin IGS: ctttttctctcataagtccacaatgaatcgccacactatacaacaaaacttaagaaattattaaaatttttagcgtgttgccattattctcccaatgatactcaattaaaaataagggtagtttgcacatttatttcaacaataagccgcattcctcgctttttatgctgaagcgtgtagaatagtgcggttatttttttatgcgttttaatgagaaaact .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agttttctcattaaaacgcataaaaaaataaccgcactattctacacgcttcagcataaaaagcgaggaatgcggcttattgttgaaataaatgtgcaaactacccttatttttaattgagtatcattgggagaataatggcaacacgctaaaaattttaataatttcttaagttttgttgtatagtgtggcgattcattgtggacttatgagagaaaaag Mask Tandem Repeat Region ================================================ ctttttctctcataagtccacaatgaatcgccacactatacaacaaaacttaagaaattattaaaatttttagcgtgttgccattattctcccaatgatactcaattaaaaataagggtagtttgcacatttatttcaacaataagccgcattcctcgctttttatgctgaagcgtgtagaatagtgcggttatttttttatgcgttttaatgagaaaact Find is-nt database================================================ Query_seq: AA02081:AA02082|AA02081:AA02082:periplasmic protein:glutaminyl-tRNA synthetase:->->:1416020..1416240 221 ctttttctctcataagtccacaatgaatcgccacactatacaacaaaacttaagaaattattaaaatttttagcgtgttgccattattctcccaatgatactcaattaaaaataagggtagtttgcacatttatttcaacaataagccgcattcctcgctttttatgctgaagcgtgtagaatagtgcggttatttttttatgcgttttaatgagaaaact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02081:AA02082|AA02081:AA02082:periplasmic protein:glutaminyl-tRNA synthetase:->->:1416020..1416240 221 ctttttctctcataagtccacaatgaatcgccacactatacaacaaaacttaagaaattattaaaatttttagcgtgttgccattattctcccaatgatactcaattaaaaataagggtagtttgcacatttatttcaacaataagccgcattcctcgctttttatgctgaagcgtgtagaatagtgcggttatttttttatgcgttttaatgagaaaact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02081:AA02082|AA02081:AA02082:periplasmic protein:glutaminyl-tRNA synthetase:->->:1416020..1416240 221 ctttttctctcataagtccacaatgaatcgccacactatacaacaaaacttaagaaattattaaaatttttagcgtgttgccattattctcccaatgatactcaattaaaaataagggtagtttgcacatttatttcaacaataagccgcattcctcgctttttatgctgaagcgtgtagaatagtgcggttatttttttatgcgttttaatgagaaaact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ctttttctctcataagtccacaatgaatcgccacactatacaacaaaacttaagaaattattaaaatttttagcgtgttgccattattctcccaatgatactcaattaaaaataagggtagtttgcacatttatttcaacaataagccgcattcctcgctttttatgctgaagcgtgtagaatagtgcggttatttttttatgcgttttaatgagaaaact Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: - Start: 105 End: 212 ........................................................................................................ N F I L T T Q V N I E V I L R M G R K K H Q L T Y F L A T I K K H T K M ......... ctttttctctcataagtccacaatgaatcgccacactatacaacaaaacttaagaaattattaaaatttttagcgtgttgccattattctcccaatgatactcaattaaaaataagggtagtttgcacatttatttcaacaataagccgcattcctcgctttttatgctgaagcgtgtagaatagtgcggttatttttttatgcgttttaatgagaaaact Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctttttctctcataagtccacaatgaatcgccacactatacaacaaaacttaagaaattattaaaatttttagcgtgttgccattattctcccaatgatactcaattaaaaataagggtagtttgcacatttatttcaacaataagccgcattcctcgctttttatgctgaagcgtgtagaatagtgcggttatttttttatgcgttttaatgagaaaact Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02081:AA02082|AA02081:AA02082:periplasmic protein:glutaminyl-tRNA synthetase:->->:1416020..1416240 221 ctttttctctcataagtccacaatgaatcgccacactatacaacaaaacttaagaaattattaaaatttttagcgtgttgccattattctcccaatgatactcaattaaaaataagggtagtttgcacatttatttcaacaataagccgcattcctcgctttttatgctgaagcgtgtagaatagtgcggttatttttttatgcgttttaatgagaaaact Intra-Species Hit: Count: 1 Min: 1 Max: 221 Len: 221 Subject: aact_AA02081_AA02082|periplasmic protein:glutaminyl-tRNA synthetase|POSITIVE:POSITIVE|[1416020,1416240]|221 HSP 1 e-value: 1.0E-109 bit: 396.0 Len: 221 Query Start:1 Query End:221 Subject Strand: POSITIVE Subject Start: 1 Subject End: 221 ctttttctctcataagtccacaatgaatcgccacactatacaacaaaacttaagaaattattaaaatttttagcgtgttgccattattctcccaatgatactcaattaaaaataagggtagtttgcacatttatttcaacaataagccgcattcctcgctttttatgctgaagcgtgtagaatagtgcggttannnnnnnatgcgttttaatgagaaaact ctttttctctcataagtccacaatgaatcgccacactatacaacaaaacttaagaaattattaaaatttttagcgtgttgccattattctcccaatgatactcaattaaaaataagggtagtttgcacatttatttcaacaataagccgcattcctcgctttttatgctgaagcgtgtagaatagtgcggttatttttttatgcgttttaatgagaaaact Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUUUUUCUCUCAUAAGUCCACAAUGAAUCGCCACACUAUACAACAAAACUUAAGAAAUUAUUAAAAUUUUUAGCGUGUUGCCAUUAUUCUCCCAAUGAUACUCAAUUAAAAAUAAGGGUAGUUUGCACAUUUAUUUCAACAAUAAGCCGCAUUCCUCGCUUUUUAUGCUGAAGCGUGUAGAAUAGUGCGGUUAUUUUUUUAUGCGUUUUAAUGAGAAAACU ..(((((((....(((..((.((.((((.(((.((((((.(.(((...(((((((((((.....))))))).(((..(((((.(((((.......((.....))......))))).)))))..)))................))))..........((((((.......))))))))).).)))))).))).))))..)).))..)))....))))))).. (-38.32) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUUUUCUCAUUAAAACGCAUAAAAAAAUAACCGCACUAUUCUACACGCUUCAGCAUAAAAAGCGAGGAAUGCGGCUUAUUGUUGAAAUAAAUGUGCAAACUACCCUUAUUUUUAAUUGAGUAUCAUUGGGAGAAUAAUGGCAACACGCUAAAAAUUUUAAUAAUUUCUUAAGUUUUGUUGUAUAGUGUGGCGAUUCAUUGUGGACUUAUGAGAGAAAAAG ..(((((((((.....((((......(((..(((((((((...(((((((((.((.......))...))).)))....(((((((((((......((...((.(((.(((((......))))).....))))).....((....)).)).....)))))))))))...........)))...)))))))))..)))...)))).....))))))))).... (-50.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 388 193 156 229 182 aact:AA01805|5end_electron_transport_complex_protein_1216115..1216345_POSITIVE 360 209 167 122 77 aact:AA00854|5end_arginine_transport_system_permease_protein_588898..589128_POSITIVE 356 190 144 101 63 aact:AA01986|5end_pyruvate_dehydrogenase_complex_E2_1348753..1348983_POSITIVE 351 188 141 208 156 aact:AA02982|5end_pyruvate_formate-lyase_activating_enzyme_2084310..2084540_POSITIVE 349 209 163 120 72 aact:AA02034|5end_ferredoxin_NADP+_reductase_1385198..1385428_POSITIVE 342 190 147 82 21 aact:AA01857|5end_glutamate-ammonia-ligase_adenylyltransferase_1247347..1247577_POSITIVE 340 209 161 169 124 aact:AA01436|5end_fructose-bisphosphate_aldolase_class_II_962981..963211_POSITIVE 333 184 141 159 117 aact:AA01532|5end_hypothetical_protein_1028771..1029001_POSITIVE 333 184 141 93 51 aact:AA01531|5end_hypothetical_protein_1028705..1028935_POSITIVE 332 209 173 155 116 aact:AA01171|5end_LysA_activator_protein_802811..803041_POSITIVE 328 189 144 79 30 aact:AA01666|5end_penicillin-binding_protein_4;_D-alanyl-D-alanine_carboxypeptidase;_D-alanyl-D-alanine-endopeptidase_1128231..1128461_POSITIVE 324 209 162 121 72 aact:AA02673|5end_gluconate_utilization_operon_repressor_1858913..1859143_POSITIVE 323 200 154 151 113 aact:AA02536|5end_conserved_hypothetical_protein_1764854..1765084_POSITIVE 321 190 145 160 115 aact:AA02902|5end_aerobic_respiration_control_protein_2031171..2031401_POSITIVE 318 214 175 105 63 aact:AA02681|5end_hypothetical_protein_1865749..1865979_POSITIVE 316 170 121 115 66 aact:AA03016|5end_hypothetical_protein_2105144..2105374_POSITIVE 316 170 121 53 4 aact:AA03015|5end_hypothetical_protein_2105082..2105312_POSITIVE 315 211 174 65 23 aact:AA00582|5end_conserved_hypothetical_protein_397904..398134_POSITIVE 310 190 145 203 165 aact:AA00310|5end_conserved_hypothetical_protein_210193..210423_POSITIVE 306 190 146 175 122 aact:AA03008|5end_GTP-binding_protein_era_homolog_(GTPase)_2100087..2100317_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 356 190 144 69 31 aact:AA01985|3end_pyruvate_dehydrogenase,_E1_component_1348801..1348951_POSITIVE 351 188 141 63 11 aact:AA02980|3end_conserved_hypothetical_protein_2084245..2084395_POSITIVE 349 209 163 136 88 aact:AA02032|3end_hypothetical_protein_1385294..1385444_POSITIVE 348 209 166 88 47 aact:AA00523|3end_conserved_hypothetical_protein_358929..359079_POSITIVE 340 209 161 129 84 aact:AA01434|3end_conserved_hypothetical_protein_(possible_o-antigen_ligase)_963021..963171_POSITIVE 333 184 141 57 15 aact:AA01530|3end_conserved_hypothetical_protein_1028749..1028899_POSITIVE 323 209 167 45 5 aact:AA02671|3end_gluconokinase_1858917..1859067_POSITIVE 323 200 154 78 40 aact:AA02534|3end_zinc_protease_1764861..1765011_POSITIVE 321 190 145 47 2 aact:AA02899|3end_oligopeptide_ABC_transporter,_ATP-binding_protein_2031138..2031288_POSITIVE 318 214 175 87 45 aact:AA02679|3end_formate_dehydrogenase,_gamma_subunit_1865811..1865961_POSITIVE 317 205 164 58 1 aact:AA01804|3end_sugar_nucleotide_epimerase;_cell_division_inhibitor_1216001..1216151_POSITIVE 316 170 121 142 93 aact:AA03015|3end_hypothetical_protein_2105251..2105401_POSITIVE 315 211 174 47 5 aact:AA00581|3end_thiosulfate_sulfurtransferase_(rhodanese)_397966..398116_POSITIVE 305 190 146 60 14 aact:AA01260|3end_hypothetical_protein_855758..855908_POSITIVE 304 198 154 77 37 aact:AA02286|3end_sugar_ABC_transporter,_ATP-binding_protein_1558536..1558686_POSITIVE 303 209 161 72 14 aact:AA00890|3end_conserved_hypothetical_protein_618054..618204_POSITIVE 301 209 166 55 7 aact:AA00718|3end_ABC-related_ATP-binding_protein;_MRP_protein_494723..494873_POSITIVE 296 193 166 145 113 aact:AA00719|3end_hypothetical_protein_494829..494979_POSITIVE 295 209 166 70 32 aact:AA02758|3end_3-phosphoshikimate_1-carboxyvinyltransferase_1933150..1933300_POSITIVE 295 189 146 61 3 aact:AA02407|3end_thymidine_kinase_1653240..1653390_POSITIVE