Origin IGS: aattggttactctaaatgatccattctcccttcggataactaactcgcaatgcattttttaatgtggagggagccaatccagacttaactgaaagcgaatgcaatgtaatgtttcgctcatgtaactcgtaaacaatccgctttctactccaatccttttttttcatagttcctcgcttttgcatttgaataatttaaagattaggttaaacttgggtgtaaatctttagtcttaccccttagcgttaaacttagtttaaacctaaagtcaacaatagtttaaataaaaattttgtaaaacttatgtaaagaaatttatttttatttaaatcaatacgttaataggtattgtaaattaaacttagttttaatccttggtttaaaactaggaggttagat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atctaacctcctagttttaaaccaaggattaaaactaagtttaatttacaatacctattaacgtattgatttaaataaaaataaatttctttacataagttttacaaaatttttatttaaactattgttgactttaggtttaaactaagtttaacgctaaggggtaagactaaagatttacacccaagtttaacctaatctttaaattattcaaatgcaaaagcgaggaactatgaaaaaaaaggattggagtagaaagcggattgtttacgagttacatgagcgaaacattacattgcattcgctttcagttaagtctggattggctccctccacattaaaaaatgcattgcgagttagttatccgaagggagaatggatcatttagagtaaccaatt Mask Tandem Repeat Region ================================================ aattggttactctaaatgatccattctcccttcggataactaactcgcaatgcattttttaatgtggagggagccaatccagacttaactgaaagcgaatgcaatgtaatgtttcgctcatgtaactcgtaaacaatccgctttctactccaatccttttttttcatagttcctcgcttttgcatttgaataatttaaagattaggttaaacttgggtgtaaatctttagtcttaccccttagcgttaaacttagtttaaacctaaagtcaacaatagtttaaataaaaattttgtaaaacttatgtaaagaaatttatttttatttaaatcaatacgttaataggtattgtaaattaaacttagttttaatccttggtttaaaactaggaggttagat Find is-nt database================================================ Query_seq: AA02071:AA02072|AA02071:AA02072:conserved hypothetical protein:conserved hypothetical protein:->->:1408342..1408740 399 aattggttactctaaatgatccattctcccttcggataactaactcgcaatgcattttttaatgtggagggagccaatccagacttaactgaaagcgaatgcaatgtaatgtttcgctcatgtaactcgtaaacaatccgctttctactccaatccttttttttcatagttcctcgcttttgcatttgaataatttaaagattaggttaaacttgggtgtaaatctttagtcttaccccttagcgttaaacttagtttaaacctaaagtcaacaatagtttaaataaaaattttgtaaaacttatgtaaagaaatttatttttatttaaatcaatacgttaataggtattgtaaattaaacttagttttaatccttggtttaaaactaggaggttagat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02071:AA02072|AA02071:AA02072:conserved hypothetical protein:conserved hypothetical protein:->->:1408342..1408740 399 aattggttactctaaatgatccattctcccttcggataactaactcgcaatgcattttttaatgtggagggagccaatccagacttaactgaaagcgaatgcaatgtaatgtttcgctcatgtaactcgtaaacaatccgctttctactccaatccttttttttcatagttcctcgcttttgcatttgaataatttaaagattaggttaaacttgggtgtaaatctttagtcttaccccttagcgttaaacttagtttaaacctaaagtcaacaatagtttaaataaaaattttgtaaaacttatgtaaagaaatttatttttatttaaatcaatacgttaataggtattgtaaattaaacttagttttaatccttggtttaaaactaggaggttagat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02071:AA02072|AA02071:AA02072:conserved hypothetical protein:conserved hypothetical protein:->->:1408342..1408740 399 aattggttactctaaatgatccattctcccttcggataactaactcgcaatgcattttttaatgtggagggagccaatccagacttaactgaaagcgaatgcaatgtaatgtttcgctcatgtaactcgtaaacaatccgctttctactccaatccttttttttcatagttcctcgcttttgcatttgaataatttaaagattaggttaaacttgggtgtaaatctttagtcttaccccttagcgttaaacttagtttaaacctaaagtcaacaatagtttaaataaaaattttgtaaaacttatgtaaagaaatttatttttatttaaatcaatacgttaataggtattgtaaattaaacttagttttaatccttggtttaaaactaggaggttagat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 3 Min: 6 Max: 167 Len: 162 Subject: UniRef90_Q7N1Q1 Cluster: Similar to sugar fermentation stimulation protein B; n=1; Photorhabdus luminescens subsp. laumondii|Rep: Similar to sugar fermentation stimulation protein B - Photorhabdus luminescens subsp. laumondii HSP 1 e-value: 3.0E-6 bit: 53.1 Len: 162 Query Start:6 Query End:167 Subject Strand: null Subject Start: 1 Subject End: 54 ..... M K K K D W S R K R I V Y E L H E R N I T L H S L S V K S G L A P S T L K N A L R V S Y P K G E W I I * S N ........................................................................................................................................................................................................................................ ..... M N K K D W H T A D I I A A L R K L G T T L A A V S R K E G L S S S T L A N A L S R P W P K G E W I I A N N ........................................................................................................................................................................................................................................ Subject: UniRef90_Q8ZMQ5 Cluster: Putative cytoplasmic protein; n=1; Salmonella typhimurium|Rep: Putative cytoplasmic protein - Salmonella typhimurium HSP 1 e-value: 8.0E-6 bit: 51.6 Len: 153 Query Start:15 Query End:167 Subject Strand: null Subject Start: 1 Subject End: 54 .............. M K K - - - K D W S R K R I V Y E L H E R N I T L H S L S V K S G L A P S T L K N A L R V S Y P K G E W I I ........................................................................................................................................................................................................................................ .............. M K K S T T K D W H P A D I V A A L H K R G T S L A K L S R E A G L A S S T L S N T L T R P W P K G E W L I ........................................................................................................................................................................................................................................ Subject: UniRef90_Q1CEJ4 Cluster: DNA-binding protein; n=5; Yersinia|Rep: DNA-binding protein - Yersinia pestis (biovar Antiqua strain Nepal516) HSP 1 e-value: 8.0E-6 bit: 51.6 Len: 153 Query Start:15 Query End:167 Subject Strand: null Subject Start: 3 Subject End: 53 .............. M K K K D W S R K R I V Y E L H E R N I T L H S L S V K S G L A P S T L K N A L R V S Y P K G E W I I ........................................................................................................................................................................................................................................ .............. L R K S D W H P A D I I A A L R K K G T T L A A L S R K A G L S S S T L A N A L T R P W P K G E W L I ........................................................................................................................................................................................................................................ aattgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagttcctcgcttttgcatttgaataatttaaagattaggttaaacttgggtgtaaatctttagtcttaccccttagcgttaaacttagtttaaacctaaagtcaacaatagtttaaataaaaattttgtaaaacttatgtaaagaaatttatttttatttaaatcaatacgttaataggtattgtaaattaaacttagttttaatccttggtttaaaactaggaggttagat Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: - Start: 62 End: 154 ............................................................. H P P L W D L S L Q F R I C H L T E S M Y S T F L G S E V G M ..................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 15 End: 194 .............. I I W E G K P Y S V R L A N K L T S P A L G S K V S L S H L T I N R E H L E Y V I R K R S W D K K K M T G R K Q M Q I M ............................................................................................................................................................................................................. aattgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagttcctcgcttttgcatttgaataatttaaagattaggttaaacttgggtgtaaatctttagtcttaccccttagcgttaaacttagtttaaacctaaagtcaacaatagtttaaataaaaattttgtaaaacttatgtaaagaaatttatttttatttaaatcaatacgttaataggtattgtaaattaaacttagttttaatccttggtttaaaactaggaggttagat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aattgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagttcctcgcttttgcatttgaataatttaaagattaggttaaacttgggtgtaaatctttagtcttaccccttagcgttaaacttagtttaaacctaaagtcaacaatagtttaaataaaaattttgtaaaacttatgtaaagaaatttatttttatttaaatcaatacgttaataggtattgtaaattaaacttagttttaatccttggtttaaaactaggaggttagat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02071:AA02072|AA02071:AA02072:conserved hypothetical protein:conserved hypothetical protein:->->:1408342..1408740 399 aattgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagttcctcgcttttgcatttgaataatttaaagattaggttaaacttgggtgtaaatctttagtcttaccccttagcgttaaacttagtttaaacctaaagtcaacaatagtttaaataaaaattttgtaaaacttatgtaaagaaatttatttttatttaaatcaatacgttaataggtattgtaaattaaacttagttttaatccttggtttaaaactaggaggttagat Intra-Species Hit: Count: 1 Min: 168 Max: 399 Len: 232 Subject: aact_AA02071_AA02072|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1408342,1408740]|399 HSP 1 e-value: 3.0E-56 bit: 220.0 Len: 111 Query Start:168 Query End:278 Subject Strand: POSITIVE Subject Start: 168 Subject End: 278 .......................................................................................................................................................................agttcctcgcttttgcatttgaataatttaaagattaggttaaacttgggtgtaaatctttagtcttaccccttagcgttaaacttagtttaaacctaaagtcaacaatag......................................................................................................................... .......................................................................................................................................................................agttcctcgcttttgcatttgaataatttaaagattaggttaaacttgggtgtaaatctttagtcttaccccttagcgttaaacttagtttaaacctaaagtcaacaatag......................................................................................................................... HSP 2 e-value: 1.0E-30 bit: 135.0 Len: 68 Query Start:332 Query End:399 Subject Strand: POSITIVE Subject Start: 332 Subject End: 399 ...........................................................................................................................................................................................................................................................................................................................................caatacgttaataggtattgtaaattaaacttagttttaatccttggtttaaaactaggaggttagat ...........................................................................................................................................................................................................................................................................................................................................caatacgttaataggtattgtaaattaaacttagttttaatccttggtttaaaactaggaggttagat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUUCCUCGCUUUUGCAUUUGAAUAAUUUAAAGAUUAGGUUAAACUUGGGUGUAAAUCUUUAGUCUUACCCCUUAGCGUUAAACUUAGUUUAAACCUAAAGUCAACAAUAGUUUAAAUAAAAAUUUUGUAAAACUUAUGUAAAGAAAUUUAUUUUUAUUUAAAUCAAUACGUUAAUAGGUAUUGUAAAUUAAACUUAGUUUUAAUCCUUGGUUUAAAACUAGGAGGUUAGAU ...........((((((((..(.(((((((.....)))))))...)..))))))))......((((.(((.(((((..(((((((.((.(((((.((((.((........((((((((((((((.((.....((....)).....))...))))))))))))))((((((.(....).))))))........)))))).)))))..)).)))))))..))))).))).)))) (-44.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUCUAACCUCCUAGUUUUAAACCAAGGAUUAAAACUAAGUUUAAUUUACAAUACCUAUUAACGUAUUGAUUUAAAUAAAAAUAAAUUUCUUUACAUAAGUUUUACAAAAUUUUUAUUUAAACUAUUGUUGACUUUAGGUUUAAACUAAGUUUAACGCUAAGGGGUAAGACUAAAGAUUUACACCCAAGUUUAACCUAAUCUUUAAAUUAUUCAAAUGCAAAAGCGAGGAACU ......((((.(((((((((.(....).))))))))).(..(((((((.........((((((...((.(((((((((((((......(((.....))).........))))))))))))).)).))))))..(((((((.(((((.(((.....)))..((((((((.(....).))))).))).))))))))))))....)))))))..)............)))).... (-43.56) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 224 88 41 119 62 aact:AA02688|5end_hydrogenase_4_component_E_1873977..1874207_POSITIVE 218 80 31 104 60 aact:AA01861|5end_phosphomethylpyrimidine_kinase_1251806..1252036_POSITIVE 215 81 45 59 25 aact:AA01704|5end_tyrosine-specific_transport_protein_1156170..1156400_POSITIVE 215 214 178 179 147 aact:AA00363|5end_50S_ribosomal_protein_L22_245571..245801_POSITIVE 213 77 38 164 123 aact:AA00529|5end_outer_membrane_protein,_Omp_18/16_361730..361960_POSITIVE 210 71 35 43 9 aact:AA00161|5end_pilin_B_biogenesis_protein;_fimbrial_transcription_regulation_113384..113614_POSITIVE 207 79 33 137 88 aact:AA01155|5end_hypothetical_protein_791227..791457_POSITIVE 206 73 31 136 88 aact:AA02236|5end_stringent_starvation_protein_A_1521919..1522149_POSITIVE 206 80 37 137 88 aact:AA01033|5end_Na(+)-translocating_NADH-quinone_reductase_subunit_A_722168..722398_POSITIVE 205 80 31 210 158 aact:AA01008|5end_mazG_beta-lactamase_regulatory_protein_homolog_703470..703700_POSITIVE 204 83 46 65 32 aact:AA02088|5end_glycogen_operon_protein_1421282..1421512_POSITIVE 203 50 7 131 73 aact:AA03014|5end_conserved_hypothetical_protein_2104815..2105045_POSITIVE 202 47 3 143 95 aact:AA01778|5end_aminopeptidase_A/I;_cytosol_aminopeptidase;_leucyl_aminopeptidase_1198398..1198628_POSITIVE 202 80 37 143 111 aact:AA00274|5end_50S_ribosomal_protein_L32_192449..192679_POSITIVE 201 77 37 100 61 aact:AA02433|5end_hypothetical_protein_1678300..1678530_POSITIVE 201 65 31 160 126 aact:AA00865|5end_conserved_hypothetical_protein_599473..599703_POSITIVE 200 58 15 135 97 aact:AA00535|5end_hypothetical_protein_365695..365925_POSITIVE 198 79 31 107 64 aact:AA02797|5end_carbohydrate_kinase;_myo-inositol_catabolism_protein_1963883..1964113_POSITIVE 198 69 32 58 21 aact:AA02516|5end_ferric_uptake_regulation_protein_1749264..1749494_POSITIVE 198 83 47 120 85 aact:AA01496|5end_glucose_inhibited_division_protein_A_1005125..1005355_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 224 88 41 105 48 aact:AA02687|3end_hydrogenase-4_component_D_1874043..1874193_POSITIVE 218 80 31 108 64 aact:AA01859|3end_hypothetical_protein_1251890..1252040_POSITIVE 213 77 38 57 16 aact:AA00528|3end_conserved_hypothetical_protein_361703..361853_POSITIVE 206 73 31 121 73 aact:AA02235|3end_stringent_starvation_protein_B_1521984..1522134_POSITIVE 206 80 37 131 82 aact:AA01032|3end_Na(+)-translocating_NADH-quinone_reductase_subunit_B_722242..722392_POSITIVE 205 80 31 106 54 aact:AA01006|3end_conserved_hypothetical_protein_(possible_cytosine_deaminas)_703446..703596_POSITIVE 203 50 7 82 24 aact:AA03013|3end_ribonuclease_P_protein_component_2104846..2104996_POSITIVE 203 80 31 143 83 aact:AA01463|3end_probable_transport_protein_984200..984350_POSITIVE 202 80 37 113 81 aact:AA00273|3end_fatty_acid/phospholipid_synthesis_protein_192499..192649_POSITIVE 201 77 37 45 6 aact:AA02432|3end_penicillin-binding_protein_1B_1678325..1678475_POSITIVE 198 79 31 61 18 aact:AA02796|3end_possible_transcriptional_regulator,_RpiR_family_1963917..1964067_POSITIVE 197 79 32 65 19 aact:AA02969|3end_beta_tyrosinase_(tyrosine_phenol-lyase)_2076417..2076567_POSITIVE 196 102 61 113 77 aact:AA01137|3end_anaerobic_dimethyl_sulfoxide_reductase,_chain_A_781057..781207_POSITIVE 194 69 38 36 4 aact:AA02515|3end_flavodoxin_1749322..1749472_POSITIVE 193 80 38 113 49 aact:AA02545|3end_conserved_hypothetical_protein_1774221..1774371_POSITIVE 191 227 181 113 70 aact:AA02190|3end_hydrogenase-2_component_protein_1493271..1493421_POSITIVE 190 80 32 98 47 aact:AA02609|3end_ABC_transporter,_ATP-binding_protein/permease_1820184..1820334_POSITIVE 189 79 33 60 14 aact:AA00109|3end_conserved_hypothetical_protein_79153..79303_POSITIVE 187 85 56 119 84 aact:AA00861|3end_transcription_repair_coupling_factor_596150..596300_POSITIVE 186 80 33 63 10 aact:AA02443|3end_cytochrome_C_related_protein_1686590..1686740_POSITIVE