Origin IGS: tttaacgaatgttttttttaaataaaggcttttcttttgaaaagcactattaacgatgggttaataatccgatttatttgagagataaagtagctttaaggaaaggcttctcctataatctggtctcttgttaataatatgaaggaaaaatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aatttttccttcatattattaacaagagaccagattataggagaagcctttccttaaagctactttatctctcaaataaatcggattattaacccatcgttaatagtgcttttcaaaagaaaagcctttatttaaaaaaaacattcgttaaa Mask Tandem Repeat Region ================================================ tttaacgaatgttttttttaaataaaggcttttcttttgaaaagcactattaacgatgggttaataatccgatttatttgagagataaagtagctttaaggaaaggcttctcctataatctggtctcttgttaataatatgaaggaaaaatt Find is-nt database================================================ Query_seq: AA02025:AA02026|AA02025:AA02026:hypothetical protein:probable serine proteinase:->->:1378912..1379063 152 tttaacgaatgttttttttaaataaaggcttttcttttgaaaagcactattaacgatgggttaataatccgatttatttgagagataaagtagctttaaggaaaggcttctcctataatctggtctcttgttaataatatgaaggaaaaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02025:AA02026|AA02025:AA02026:hypothetical protein:probable serine proteinase:->->:1378912..1379063 152 tttaacgaatgttttttttaaataaaggcttttcttttgaaaagcactattaacgatgggttaataatccgatttatttgagagataaagtagctttaaggaaaggcttctcctataatctggtctcttgttaataatatgaaggaaaaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02025:AA02026|AA02025:AA02026:hypothetical protein:probable serine proteinase:->->:1378912..1379063 152 tttaacgaatgttttttttaaataaaggcttttcttttgaaaagcactattaacgatgggttaataatccgatttatttgagagataaagtagctttaaggaaaggcttctcctataatctggtctcttgttaataatatgaaggaaaaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tttaacgaatgttttttttaaataaaggcttttcttttgaaaagcactattaacgatgggttaataatccgatttatttgagagataaagtagctttaaggaaaggcttctcctataatctggtctcttgttaataatatgaaggaaaaatt Predict ORF larger than 30AA ================================================ tttaacgaatgttttttttaaataaaggcttttcttttgaaaagcactattaacgatgggttaataatccgatttatttgagagataaagtagctttaaggaaaggcttctcctataatctggtctcttgttaataatatgaaggaaaaatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tttaacgaatgttttttttaaataaaggcttttcttttgaaaagcactattaacgatgggttaataatccgatttatttgagagataaagtagctttaaggaaaggcttctcctataatctggtctcttgttaataatatgaaggaaaaatt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 72 HP_score: -8.5 Tail_Score: -3.24488 Start: 28 End: 45 Full_Region: cccatcgttaatagt gcttttc aaaa gaaaagc ctttatttaaaaaaa ...........................gcttttcaaaagaaaagc........................................................................................................... TransTerm Strand: - Conf: 55 HP_score: -3.1 Tail_Score: -4.07568 Start: 44 End: 70 Full_Region: tctctcaaataaatc ggattattaac ccatc gttaatagtgc ttttcaaaagaaaag ...........................................ggattattaacccatcgttaatagtgc.................................................................................. TransTerm Strand: - Conf: 45 HP_score: -3.4 Tail_Score: -3.08032 Start: 47 End: 67 Full_Region: ctcaaataaatcgga ttattaac ccatc gttaatag tgcttttcaaaagaa ..............................................ttattaacccatcgttaatag..................................................................................... Find igs database================================================ Query_seq: AA02025:AA02026|AA02025:AA02026:hypothetical protein:probable serine proteinase:->->:1378912..1379063 152 tttaacgaatgttttttttaaataaaggcttttcttttgaaaagcactattaacgatgggttaataatccgatttatttgagagataaagtagctttaaggaaaggcttctcctataatctggtctcttgttaataatatgaaggaaaaatt Intra-Species Hit: Count: 1 Min: 20 Max: 152 Len: 133 Subject: aact_AA02025_AA02026|hypothetical protein:probable serine proteinase|POSITIVE:POSITIVE|[1378912,1379063]|152 HSP 1 e-value: 7.0E-70 bit: 264.0 Len: 133 Query Start:20 Query End:152 Subject Strand: POSITIVE Subject Start: 20 Subject End: 152 ...................aaataaaggcttttcttttgaaaagcactattaacgatgggttaataatccgatttatttgagagataaagtagctttaaggaaaggcttctcctataatctggtctcttgttaataatatgaaggaaaaatt ...................aaataaaggcttttcttttgaaaagcactattaacgatgggttaataatccgatttatttgagagataaagtagctttaaggaaaggcttctcctataatctggtctcttgttaataatatgaaggaaaaatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAUAAAGGCUUUUCUUUUGAAAAGCACUAUUAACGAUGGGUUAAUAAUCCGAUUUAUUUGAGAGAUAAAGUAGCUUUAAGGAAAGGCUUCUCCUAUAAUCUGGUCUCUUGUUAAUAAUAUGAAGGAAAAAUU ........(((((((....)))))))..(((((((((.(((........(((..((((..((((........((((((.....))))))))))..))))..))).))))))))))))................ (-27.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUUUUUCCUUCAUAUUAUUAACAAGAGACCAGAUUAUAGGAGAAGCCUUUCCUUAAAGCUACUUUAUCUCUCAAAUAAAUCGGAUUAUUAACCCAUCGUUAAUAGUGCUUUUCAAAAGAAAAGCCUUUAUUU ..............(((((((((.(((((..((.....((((((....)))))).....))......)))))..........((.........))...)))))))))(((((((....)))))))........ (-22.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 255 110 62 216 168 aact:AA00573|5end_chloramphenicol-sensitive_protein_RarD_391965..392195_POSITIVE 250 95 59 214 179 aact:AA00241|5end_formate_acetyltransferase_activating_enzyme_(anaerobic_ribonucleoside-triphosphate_reductase_activating_protein)_171546..171776_POSITIVE 250 95 59 58 23 aact:AA00240|5end_hypothetical_protein_171390..171620_POSITIVE 248 69 32 159 117 aact:AA00954|5end_uroporphyrinogen_III_synthase_660247..660477_POSITIVE 247 78 35 60 12 aact:AA02160|5end_conserved_hypothetical_protein_1477794..1478024_POSITIVE 245 94 58 192 156 aact:AA02491|5end_topoisomerase_IV,_subunit_A_1726827..1727057_POSITIVE 231 129 86 110 65 aact:AA01547|5end_deoxyribose-phosphate_aldolase_1036271..1036501_POSITIVE 229 43 8 62 26 aact:AA02272|5end_conserved_hypothetical_protein_1548798..1549028_POSITIVE 229 59 18 198 155 aact:AA00356|5end_DNA-directed_RNA_polymerase,_beta'_chain_238937..239167_POSITIVE 218 111 74 168 137 aact:AA00424|5end_hypothetical_protein_287123..287353_POSITIVE 217 107 79 148 124 aact:AA01983|5end_maltose_regulon_positive_regulatory_protein_1341947..1342177_POSITIVE 212 50 7 185 147 aact:AA02785|5end_ribose_ABC_transporter,_permease_1951808..1952038_POSITIVE 211 95 61 194 165 aact:AA00766|5end_heat_shock_protein_B25.3_(HSP-70_cofactor)_527384..527614_POSITIVE 210 67 24 84 42 aact:AA00034|5end_NTP-binding_protein_26374..26604_POSITIVE 206 104 71 212 181 aact:AA02921|5end_conserved_hypothetical_protein_(cell_filamentation_protein)_2045674..2045904_POSITIVE 206 88 49 123 78 aact:AA01332|5end_galactosyltransferase_II_898875..899105_POSITIVE 203 70 25 58 16 aact:AA01611|5end_anthranilate_synthase_component_II_1088976..1089206_POSITIVE 203 110 61 179 124 aact:AA00982|5end_aldo-keto_reductase_686526..686756_POSITIVE 203 88 49 221 186 aact:AA00863|5end_conserved_hypothetical_protein_596679..596909_POSITIVE 202 55 32 231 208 aact:AA02388|5end_alpha-glucosidase_1636138..1636368_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 300 120 74 127 92 aact:AA00314|3end_conserved_hypothetical_protein_(possible_Kmt1_protein)_214326..214476_POSITIVE 248 69 32 125 83 aact:AA00953|3end_uroporphyrinogen_III_methylase_660293..660443_POSITIVE 242 120 71 87 30 aact:AA01357|3end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919785..919935_POSITIVE 231 129 86 79 34 aact:AA01546|3end_deoxyribose_operon_repressor_1036320..1036470_POSITIVE 229 59 18 92 49 aact:AA00355|3end_DNA-directed_RNA_polymerase_beta_chain_(transcriptase_beta_chain)_238911..239061_POSITIVE 218 92 52 107 73 aact:AA02081|3end_periplasmic_protein_1415959..1416109_POSITIVE 212 50 7 96 58 aact:AA02784|3end_NAD+_dependent_acetaldehyde-alcohol_dehydrogenase,_iron-containing_1951799..1951949_POSITIVE 211 95 61 77 48 aact:AA00764|3end_hemoglobin_binding_protein_A_527347..527497_POSITIVE 210 67 24 123 81 aact:AA00033|3end_conserved_hypothetical_protein_26493..26643_POSITIVE 200 113 75 137 101 aact:AA00365|3end_30S_ribosomal_protein_S19_246266..246416_POSITIVE 198 75 34 44 8 aact:AA02220|3end_ABC_transport_system_permease_protein_1512236..1512386_POSITIVE 197 42 9 138 103 aact:AA01966|3end_2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_pyrophosphokinase_1331514..1331664_POSITIVE 196 89 45 81 33 aact:AA01599|3end_phosphocarrier_protein_HPr_1080444..1080594_POSITIVE 196 54 15 144 105 aact:AA01514|3end_peroxiredoxin_2_family_protein/glutaredoxin_1020695..1020845_POSITIVE 195 116 74 85 44 aact:AA02099|3end_50S_ribosomal_protein_L25_1430003..1430153_POSITIVE 194 125 84 97 41 aact:AA01731|3end_multidrug_resistance_protein_B_1170675..1170825_POSITIVE 192 68 32 73 39 aact:AA00568|3end_hypothetical_protein_391276..391426_POSITIVE 191 88 52 47 1 aact:AA02748|3end_conserved_hypothetical_protein_1925582..1925732_POSITIVE 190 114 77 83 47 aact:AA02597|3end_ribonuclease_D_1808358..1808508_POSITIVE 190 122 88 136 93 aact:AA01876|3end_PTS_system,_IIBC_components_1264131..1264281_POSITIVE