Origin IGS: taaaagtgcggtggttttcctcagtgtttcttattctccctaggcgtgtttagtcaatacgttggcatgctattctctcccaaatttttcattaaactgaaaactaaatccactatctgaattgtgcatagtgttaagcgtgatcatgatcacaatattcgaaaattgtccgcgtctttgtttcctttgatttcgtttgctatgataaaatgagcataaacgaacatttagatttaaaaatgaacagatttatatcaaattcgaggtggaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttccacctcgaatttgatataaatctgttcatttttaaatctaaatgttcgtttatgctcattttatcatagcaaacgaaatcaaaggaaacaaagacgcggacaattttcgaatattgtgatcatgatcacgcttaacactatgcacaattcagatagtggatttagttttcagtttaatgaaaaatttgggagagaatagcatgccaacgtattgactaaacacgcctagggagaataagaaacactgaggaaaaccaccgcactttta Mask Tandem Repeat Region ================================================ taaaagtgcggtggttttcctcagtgtttcttattctccctaggcgtgtttagtcaatacgttggcatgctattctctcccaaatttttcattaaactgaaaactaaatccactatctgaattgtgcatagtgttaagcgtgatcatgatcacaatattcgaaaattgtccgcgtctttgtttcctttgatttcgtttgctatgataaaatgagcataaacgaacatttagatttaaaaatgaacagatttatatcaaattcgaggtggaaa Find is-nt database================================================ Query_seq: AA02020:AA02021|AA02020:AA02021:conserved hypothetical protein (possible tetrapyrrole methylase family protein):anaerobic glycerol-3-phosphate dehydrogenase:->->:1374803..1375074 272 taaaagtgcggtggttttcctcagtgtttcttattctccctaggcgtgtttagtcaatacgttggcatgctattctctcccaaatttttcattaaactgaaaactaaatccactatctgaattgtgcatagtgttaagcgtgatcatgatcacaatattcgaaaattgtccgcgtctttgtttcctttgatttcgtttgctatgataaaatgagcataaacgaacatttagatttaaaaatgaacagatttatatcaaattcgaggtggaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02020:AA02021|AA02020:AA02021:conserved hypothetical protein (possible tetrapyrrole methylase family protein):anaerobic glycerol-3-phosphate dehydrogenase:->->:1374803..1375074 272 taaaagtgcggtggttttcctcagtgtttcttattctccctaggcgtgtttagtcaatacgttggcatgctattctctcccaaatttttcattaaactgaaaactaaatccactatctgaattgtgcatagtgttaagcgtgatcatgatcacaatattcgaaaattgtccgcgtctttgtttcctttgatttcgtttgctatgataaaatgagcataaacgaacatttagatttaaaaatgaacagatttatatcaaattcgaggtggaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02020:AA02021|AA02020:AA02021:conserved hypothetical protein (possible tetrapyrrole methylase family protein):anaerobic glycerol-3-phosphate dehydrogenase:->->:1374803..1375074 272 taaaagtgcggtggttttcctcagtgtttcttattctccctaggcgtgtttagtcaatacgttggcatgctattctctcccaaatttttcattaaactgaaaactaaatccactatctgaattgtgcatagtgttaagcgtgatcatgatcacaatattcgaaaattgtccgcgtctttgtttcctttgatttcgtttgctatgataaaatgagcataaacgaacatttagatttaaaaatgaacagatttatatcaaattcgaggtggaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaagtgcggtggttttcctcagtgtttcttattctccctaggcgtgtttagtcaatacgttggcatgctattctctcccaaatttttcattaaactgaaaactaaatccactatctgaattgtgcatagtgttaagcgtgatcatgatcacaatattcgaaaattgtccgcgtctttgtttcctttgatttcgtttgctatgataaaatgagcataaacgaacatttagatttaaaaatgaacagatttatatcaaattcgaggtggaaa Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 121 End: 210 ........................................................................................................................ M V H S V K R D H D H N I R K L S A S L F P L I S F A M I K .............................................................. Protein_Len: 36 Strand: + Start: 165 End: 272 .................................................................................................................................................................... M V R V F V S F D F V C Y D K M S I N E H L D L K M N R F I S N S R W K Protein_Len: 59 Strand: - Start: 73 End: 249 ........................................................................ E R G F K E N F Q F S F G S D S N H M T N L T I M I V I N S F Q G R R Q K R Q N R K S H Y F S C L R V N L N L F S C M ....................... taaaagtgcggtggttttcctcagtgtttcttattctccctaggcgtgtttagtcaatacgttggcatgctattctctcccaaatttttcattaaactgaaaactaaatccactatctgaattgtgcatagtgttaagcgtgatcatgatcacaatattcgaaaattgtccgcgtctttgtttcctttgatttcgtttgctatgataaaatgagcataaacgaacatttagatttaaaaatgaacagatttatatcaaattcgaggtggaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 177 End: 206 ................................................................................................................................................................................TTTGTTTCCTTTGATTTCGTTTGCTATGAT.................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaagtgcggtggttttcctcagtgtttcttattctccctaggcgtgtttagtcaatacgttggcatgctattctctcccaaatttttcattaaactgaaaactaaatccactatctgaattgtgcatagtgttaagcgtgatcatgatcacaatattcgaaaattgtccgcgtctttgtttcctttgatttcgtttgctatgataaaatgagcataaacgaacatttagatttaaaaatgaacagatttatatcaaattcgaggtggaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02020:AA02021|AA02020:AA02021:conserved hypothetical protein (possible tetrapyrrole methylase family protein):anaerobic glycerol-3-phosphate dehydrogenase:->->:1374803..1375074 272 taaaagtgcggtggttttcctcagtgtttcttattctccctaggcgtgtttagtcaatacgttggcatgctattctctcccaaatttttcattaaactgaaaactaaatccactatctgaattgtgcatagtgttaagcgtgatcatgatcacaatattcgaaaattgtccgcgtctttgtttcctttgatttcgtttgctatgataaaatgagcataaacgaacatttagatttaaaaatgaacagatttatatcaaattcgaggtggaaa Intra-Species Hit: Count: 1 Min: 1 Max: 272 Len: 272 Subject: aact_AA02020_AA02021|conserved hypothetical protein (possible tetrapyrrole methylase family protein):anaerobic glycerol-3-phosphate dehydrogenase|POSITIVE:POSITIVE|[1374803,1375074]|272 HSP 1 e-value: 1.0E-152 bit: 539.0 Len: 272 Query Start:1 Query End:272 Subject Strand: POSITIVE Subject Start: 1 Subject End: 272 taaaagtgcggtggttttcctcagtgtttcttattctccctaggcgtgtttagtcaatacgttggcatgctattctctcccaaatttttcattaaactgaaaactaaatccactatctgaattgtgcatagtgttaagcgtgatcatgatcacaatattcgaaaattgtccgcgtctttgtttcctttgatttcgtttgctatgataaaatgagcataaacgaacatttagatttaaaaatgaacagatttatatcaaattcgaggtggaaa taaaagtgcggtggttttcctcagtgtttcttattctccctaggcgtgtttagtcaatacgttggcatgctattctctcccaaatttttcattaaactgaaaactaaatccactatctgaattgtgcatagtgttaagcgtgatcatgatcacaatattcgaaaattgtccgcgtctttgtttcctttgatttcgtttgctatgataaaatgagcataaacgaacatttagatttaaaaatgaacagatttatatcaaattcgaggtggaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAGUGCGGUGGUUUUCCUCAGUGUUUCUUAUUCUCCCUAGGCGUGUUUAGUCAAUACGUUGGCAUGCUAUUCUCUCCCAAAUUUUUCAUUAAACUGAAAACUAAAUCCACUAUCUGAAUUGUGCAUAGUGUUAAGCGUGAUCAUGAUCACAAUAUUCGAAAAUUGUCCGCGUCUUUGUUUCCUUUGAUUUCGUUUGCUAUGAUAAAAUGAGCAUAAACGAACAUUUAGAUUUAAAAAUGAACAGAUUUAUAUCAAAUUCGAGGUGGAAA ..(((((((((((((((((.....(((.((.............(((((((.....)))))))(((((((((..............((((((......)))))).......(((((((........).))))))...)))))).))).))..)))......)))))))).))))).)))).((((((((((.((((((((((((......)).))))..))))))......(((.((((..(....)..)))).)))....)))))).)))). (-52.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUCCACCUCGAAUUUGAUAUAAAUCUGUUCAUUUUUAAAUCUAAAUGUUCGUUUAUGCUCAUUUUAUCAUAGCAAACGAAAUCAAAGGAAACAAAGACGCGGACAAUUUUCGAAUAUUGUGAUCAUGAUCACGCUUAACACUAUGCACAAUUCAGAUAGUGGAUUUAGUUUUCAGUUUAAUGAAAAAUUUGGGAGAGAAUAGCAUGCCAACGUAUUGACUAAACACGCCUAGGGAGAAUAAGAAACACUGAGGAAAACCACCGCACUUUUA (((((.....((((..(((....))).)))).................((((((..((((...........))))))))))......))))).......((((...((((((.......(((((....)))))......((((((.(........))))))).......((((((......))))))..(((((......((((((((....))).)).)))......))))).))))))............((....)).))))....... (-45.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 386 48 3 135 94 aact:AA01463|5end_probable_transport_protein_982732..982962_POSITIVE 362 48 2 179 143 aact:AA00084|5end_aminotransferase_60814..61044_POSITIVE 348 48 4 193 154 aact:AA00311|5end_L-fuculose-phosphate_aldolase_211434..211664_POSITIVE 341 46 2 102 58 aact:AA01338|5end_hypothetical_protein_904967..905197_POSITIVE 339 200 156 225 180 aact:AA00713|5end_chaperone_protein_hscA,_heat_shock_cognate_66_488770..489000_POSITIVE 338 45 3 107 71 aact:AA00065|5end_hypothetical_protein_48854..49084_POSITIVE 337 29 2 152 125 aact:AA01824|5end_iron(III)_ABC_transporter,_ATP-binding_protein_1227705..1227935_POSITIVE 337 46 3 82 45 aact:AA00595|5end_hypothetical_protein_407194..407424_POSITIVE 334 82 43 145 102 aact:AA01260|5end_hypothetical_protein_855550..855780_POSITIVE 332 47 2 173 133 aact:AA00557|5end_nitrate/nitrite_sensor_protein_380408..380638_POSITIVE 327 46 2 143 100 aact:AA00250|5end_lipoate_biosynthesis_protein_A_175496..175726_POSITIVE 324 48 1 171 126 aact:AA01171|5end_LysA_activator_protein_802811..803041_POSITIVE 324 47 1 195 140 aact:AA00511|5end_nicotinamide_mononucleotide_transporter_353884..354114_POSITIVE 323 50 3 217 170 aact:AA01793|5end_conserved_hypothetical_protein_1208627..1208857_POSITIVE 319 200 166 226 189 aact:AA02090|5end_glucose-1-phosphate_adenylyltransferase_1423162..1423392_POSITIVE 319 29 2 84 57 aact:AA00906|5end_sigma-E_factor_regulatory_protein_625110..625340_POSITIVE 318 48 6 111 58 aact:AA01325|5end_formamidopyrimidine-DNA_glycosylase_894747..894977_POSITIVE 317 48 2 229 177 aact:AA01883|5end_recycling_protein;_permease_1269821..1270051_POSITIVE 314 47 5 118 68 aact:AA01998|5end_16S_rRNA_pseudouridine_synthase_1356842..1357072_POSITIVE 312 48 1 149 111 aact:AA00174|5end_ribosomal_protein_L11_methyltransferase_123072..123302_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 398 50 4 116 62 aact:AA00523|3end_conserved_hypothetical_protein_358929..359079_POSITIVE 386 48 3 102 61 aact:AA01462|3end_xylose_operon_regulatory_protein_982779..982929_POSITIVE 362 48 2 136 100 aact:AA00083|3end_hypothetical_protein_60851..61001_POSITIVE 362 48 2 52 16 aact:AA00081|3end_fatty_acid_metabolism_regulator_protein_60767..60917_POSITIVE 348 79 34 80 37 aact:AA01566|3end_conserved_hypothetical_protein_1052091..1052241_POSITIVE 348 79 34 80 37 aact:AA01261|3end_conserved_hypothetical_protein_856723..856873_POSITIVE 341 46 2 86 42 aact:AA01337|3end_cell_division_protein_N_905031..905181_POSITIVE 338 45 3 119 83 aact:AA00064|3end_ribosomal_small_subunit_pseudouridine_synthase_48946..49096_POSITIVE 337 29 2 114 87 aact:AA01823|3end_hypothetical_protein_1227747..1227897_POSITIVE 337 29 2 38 11 aact:AA01822|3end_lysostaphin_precursor_1227671..1227821_POSITIVE 337 46 3 94 57 aact:AA00592|3end_conserved_hypothetical_protein_(membrane/transport_protein)_407286..407436_POSITIVE 334 82 43 116 73 aact:AA01565|3end_conserved_hypothetical_protein_1050967..1051117_POSITIVE 332 47 2 127 87 aact:AA00555|3end_hypothetical_protein_380442..380592_POSITIVE 324 47 1 114 59 aact:AA00508|3end_SAM-dependent_methyltransferase_353883..354033_POSITIVE 323 50 3 97 50 aact:AA01792|3end_conserved_hypothetical_protein_1208587..1208737_POSITIVE 317 48 2 93 41 aact:AA01881|3end_adenylate_kinase_1269765..1269915_POSITIVE 314 47 5 60 10 aact:AA01997|3end_probable_translation_factor_1356864..1357014_POSITIVE 312 48 1 134 96 aact:AA00173|3end_hypothetical_protein_123137..123287_POSITIVE 310 29 2 85 58 aact:AA02210|3end_ribonucleoside-diphosphate_reductase_alpha_chain_1505956..1506106_POSITIVE 310 48 2 123 53 aact:AA01145|3end_glycyl-tRNA_synthetase_alpha_subunit_784460..784610_POSITIVE