Origin IGS: taatcgctgtttagcgtaatcttatacagaaaagggcgggtatagcccgcctttatgataaaaaaacatctttaaaagcaaccgcacttttggtgggttatcttaggatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatcctaagataacccaccaaaagtgcggttgcttttaaagatgtttttttatcataaaggcgggctatacccgcccttttctgtataagattacgctaaacagcgatta Mask Tandem Repeat Region ================================================ taatcgctgtttagcgtaatcttatacagaaaagggcgggtatagcccgcctttatgataaaaaaacatctttaaaagcaaccgcacttttggtgggttatcttaggatta Find is-nt database================================================ Query_seq: AA02013:AA02014|AA02013:AA02014:carbonic anhydrase:probable hemolysin:->->:1370435..1370545 111 taatcgctgtttagcgtaatcttatacagaaaagggcgggtatagcccgcctttatgataaaaaaacatctttaaaagcaaccgcacttttggtgggttatcttaggatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02013:AA02014|AA02013:AA02014:carbonic anhydrase:probable hemolysin:->->:1370435..1370545 111 taatcgctgtttagcgtaatcttatacagaaaagggcgggtatagcccgcctttatgataaaaaaacatctttaaaagcaaccgcacttttggtgggttatcttaggatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02013:AA02014|AA02013:AA02014:carbonic anhydrase:probable hemolysin:->->:1370435..1370545 111 taatcgctgtttagcgtaatcttatacagaaaagggcgggtatagcccgcctttatgataaaaaaacatctttaaaagcaaccgcacttttggtgggttatcttaggatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatcgctgtttagcgtaatcttatacagaaaagggcgggtatagcccgcctttatgataaaaaaacatctttaaaagcaaccgcacttttggtgggttatcttaggatta Predict ORF larger than 30AA ================================================ taatcgctgtttagcgtaatcttatacagaaaagggcgggtatagcccgcctttatgataaaaaaacatctttaaaagcaaccgcacttttggtgggttatcttaggatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatcgctgtttagcgtaatcttatacagaaaagggcgggtatagcccgcctttatgataaaaaaacatctttaaaagcaaccgcacttttggtgggttatcttaggatta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 79 HP_score: -7.3 Tail_Score: -4.61199 Start: 34 End: 52 Full_Region: gtttttttatcataa aggcggg ctata cccgccc ttttctgtataagat .................................aggcgggctatacccgccc........................................................... TransTerm Strand: - Conf: 82 HP_score: -10.8 Tail_Score: -3.35996 Start: 35 End: 51 Full_Region: tttttttatcataaa ggcggg ctata cccgcc cttttctgtataaga ..................................ggcgggctatacccgcc............................................................ Find igs database================================================ Query_seq: AA02013:AA02014|AA02013:AA02014:carbonic anhydrase:probable hemolysin:->->:1370435..1370545 111 taatcgctgtttagcgtaatcttatacagaaaagggcgggtatagcccgcctttatgataaaaaaacatctttaaaagcaaccgcacttttggtgggttatcttaggatta Intra-Species Hit: Count: 1 Min: 1 Max: 111 Len: 111 Subject: aact_AA02013_AA02014|carbonic anhydrase:probable hemolysin|POSITIVE:POSITIVE|[1370435,1370545]|111 HSP 1 e-value: 2.0E-44 bit: 178.0 Len: 111 Query Start:1 Query End:111 Subject Strand: POSITIVE Subject Start: 1 Subject End: 111 taatcgctgtttagcgtaatcttatacagaaaagggcgggtatagcccgcctttatgatnnnnnnncatctttaaaagcaaccgcacttttggtgggttatcttaggatta taatcgctgtttagcgtaatcttatacagaaaagggcgggtatagcccgcctttatgataaaaaaacatctttaaaagcaaccgcacttttggtgggttatcttaggatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCGCUGUUUAGCGUAAUCUUAUACAGAAAAGGGCGGGUAUAGCCCGCCUUUAUGAUAAAAAAACAUCUUUAAAAGCAACCGCACUUUUGGUGGGUUAUCUUAGGAUUA ....((((....)))).(((((((..((...(((((((((.....))))))))).))......................((((.(((.....)))))))....))))))). (-31.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCCUAAGAUAACCCACCAAAAGUGCGGUUGCUUUUAAAGAUGUUUUUUUAUCAUAAAGGCGGGCUAUACCCGCCCUUUUCUGUAUAAGAUUACGCUAAACAGCGAUUA (((((.(((((((((((((.....))).))))).))))).((.(((..((((((((.(((((((((.....)))).)))))..)).))))))..))))).......))))) (-28.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 429 57 13 205 169 aact:AA02696|5end_formate_hydrogenlyase_maturation_protein_1879476..1879706_POSITIVE 342 107 62 138 93 aact:AA01308|5end_cyclic_AMP_receptor_protein_888044..888274_POSITIVE 341 98 56 130 86 aact:AA00613|5end_lipid-A-disaccharide_synthase_418713..418943_POSITIVE 336 90 46 125 82 aact:AA01832|5end_formate_dehydrogenase_formation_protein_E_1233609..1233839_POSITIVE 332 96 61 124 90 aact:AA00508|5end_SAM-dependent_methyltransferase_353096..353326_POSITIVE 328 54 14 132 98 aact:AA02343|5end_glutamine_synthetase_1600173..1600403_POSITIVE 321 90 45 162 114 aact:AA00130|5end_mercuric_transport_protein_(periplasmic_mercury_ion_binding_protein)_(mercury_scavenger_protein)_94217..94447_POSITIVE 321 90 45 67 19 aact:AA00129|5end_hypothetical_protein_94122..94352_POSITIVE 317 49 5 103 54 aact:AA01195|5end_acyl_carrier_protein_819849..820079_POSITIVE 315 99 51 165 111 aact:AA00324|5end_tRNA_(Uracil-5-)-methyltransferase_(tRNA(M-5-U54)-methyltransferase)_220147..220377_POSITIVE 314 99 61 151 107 aact:AA00419|5end_conserved_hypothetical_protein_(possible_phosphatidylethanolamine-binding_protein)_284337..284567_POSITIVE 313 54 18 138 94 aact:AA00113|5end_uracil_phosphoribosyltransferase_81286..81516_POSITIVE 311 90 48 61 20 aact:AA02091|5end_hypothetical_protein_1424441..1424671_POSITIVE 310 49 3 175 121 aact:AA00725|5end_translation_initiation_factor_1_497981..498211_POSITIVE 310 107 61 148 94 aact:AA00401|5end_FKBP-type_peptidyl-prolyl_cis-trans_isomerase_slyD_(PPiase)_268361..268591_POSITIVE 308 108 62 169 130 aact:AA02673|5end_gluconate_utilization_operon_repressor_1858913..1859143_POSITIVE 308 108 68 180 146 aact:AA01649|5end_maltose_ABC_transporter,_permease_protein_1117316..1117546_POSITIVE 303 109 61 137 93 aact:AA02310|5end_conserved_hypothetical_protein_1579249..1579479_POSITIVE 303 94 59 126 92 aact:AA02252|5end_conserved_hypothetical_protein_1533840..1534070_POSITIVE 303 99 62 155 122 aact:AA01111|5end_conserved_hypothetical_protein_764765..764995_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 429 57 13 67 31 aact:AA02695|3end_hydrogenase-3_component_G_1879418..1879568_POSITIVE 338 110 62 140 94 aact:AA01044|3end_A/G-specific_adenine_glycosylase_727978..728128_POSITIVE 336 90 46 45 2 aact:AA01830|3end_2,3-cyclic-nucleotide_2-phosphodiesterase_1233609..1233759_POSITIVE 332 96 61 149 115 aact:AA00507|3end_hypothetical_protein_353201..353351_POSITIVE 332 96 61 37 3 aact:AA00506|3end_ATP-dependent_RNA_helicase_protein_353089..353239_POSITIVE 323 98 62 42 6 aact:AA00612|3end_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosami_ne_418705..418855_POSITIVE 321 90 45 72 24 aact:AA00128|3end_cation-transporting_ATPase_(probable_potassium/copper_transporter)_94207..94357_POSITIVE 317 49 5 97 48 aact:AA01194|3end_acyl_carrier_protein_819923..820073_POSITIVE 311 90 48 89 48 aact:AA02090|3end_glucose-1-phosphate_adenylyltransferase_1424549..1424699_POSITIVE 311 52 15 81 45 aact:AA01675|3end_probable_multidrug_resistance_protein;_multidrug-efflux_transporter_1133589..1133739_POSITIVE 310 49 3 150 96 aact:AA00724|3end_hypothetical_protein_498036..498186_POSITIVE 310 107 61 70 16 aact:AA00399|3end_conserved_hypothetical_protein_268363..268513_POSITIVE 309 54 32 59 37 aact:AA02013|3end_carbonic_anhydrase_1370374..1370524_POSITIVE 308 108 62 93 54 aact:AA02671|3end_gluconokinase_1858917..1859067_POSITIVE 308 108 68 82 48 aact:AA01648|3end_alpha-amylase_protein;_1,4-alpha-D-glucan_glucanohydrolase_1117298..1117448_POSITIVE 305 54 26 30 3 aact:AA02342|3end_ISRSO11/IS150_related_transposase,_orfB_1600151..1600301_POSITIVE 303 94 59 49 15 aact:AA02251|3end_biotin_sulfoxide_reductase;_Trimethylamine-N-oxide_reductase_precursor_1533843..1533993_POSITIVE 303 99 62 35 2 aact:AA01109|3end_oxidoreductase_(possible_short-chain_alcohol_dehydrogenase)_764725..764875_POSITIVE 301 56 13 71 29 aact:AA02027|3end_lipoprotein;_hemagglutinin_antigen_1381073..1381223_POSITIVE 301 90 47 88 32 aact:AA01992|3end_protease_IV;_signal_peptide_peptidase;_Endopeptidase_IV_1355004..1355154_POSITIVE