Origin IGS: aataactccttcataatttagcataatgaaaatggtaccttttcatatacgttagacggagtagggcgcaaatgcttacagattatttttattatttttcggttaacctaaaaattattattgtaaataataggttatgctaaaaataaattttatcaagttacgttccaagctatactatgttcctttttgatttattgcgggaataggaccgt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 acggtcctattcccgcaataaatcaaaaaggaacatagtatagcttggaacgtaacttgataaaatttatttttagcataacctattatttacaataataatttttaggttaaccgaaaaataataaaaataatctgtaagcatttgcgccctactccgtctaacgtatatgaaaaggtaccattttcattatgctaaattatgaaggagttatt Mask Tandem Repeat Region ================================================ aataactccttcataatttagcataatgaaaatggtaccttttcatatacgttagacggagtagggcgcaaatgcttacagattatttttattatttttcggttaacctaaaaattattattgtaaataataggttatgctaaaaataaattttatcaagttacgttccaagctatactatgttcctttttgatttattgcgggaataggaccgt Find is-nt database================================================ Query_seq: AA02012:AA02013|AA02012:AA02013:peptide methionine sulfoxide reductase:carbonic anhydrase:->->:1369530..1369744 215 aataactccttcataatttagcataatgaaaatggtaccttttcatatacgttagacggagtagggcgcaaatgcttacagattatttttattatttttcggttaacctaaaaattattattgtaaataataggttatgctaaaaataaattttatcaagttacgttccaagctatactatgttcctttttgatttattgcgggaataggaccgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02012:AA02013|AA02012:AA02013:peptide methionine sulfoxide reductase:carbonic anhydrase:->->:1369530..1369744 215 aataactccttcataatttagcataatgaaaatggtaccttttcatatacgttagacggagtagggcgcaaatgcttacagattatttttattatttttcggttaacctaaaaattattattgtaaataataggttatgctaaaaataaattttatcaagttacgttccaagctatactatgttcctttttgatttattgcgggaataggaccgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02012:AA02013|AA02012:AA02013:peptide methionine sulfoxide reductase:carbonic anhydrase:->->:1369530..1369744 215 aataactccttcataatttagcataatgaaaatggtaccttttcatatacgttagacggagtagggcgcaaatgcttacagattatttttattatttttcggttaacctaaaaattattattgtaaataataggttatgctaaaaataaattttatcaagttacgttccaagctatactatgttcctttttgatttattgcgggaataggaccgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aataactccttcataatttagcataatgaaaatggtaccttttcatatacgttagacggagtagggcgcaaatgcttacagattatttttattatttttcggttaacctaaaaattattattgtaaataataggttatgctaaaaataaattttatcaagttacgttccaagctatactatgttcctttttgatttattgcgggaataggaccgt Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 45 End: 140 ............................................ M Y V R R S R A Q M L T D Y F Y Y F S V N L K I I I V N N R L C ........................................................................... aataactccttcataatttagcataatgaaaatggtaccttttcatatacgttagacggagtagggcgcaaatgcttacagattatttttattatttttcggttaacctaaaaattattattgtaaataataggttatgctaaaaataaattttatcaagttacgttccaagctatactatgttcctttttgatttattgcgggaataggaccgt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aataactccttcataatttagcataatgaaaatggtaccttttcatatacgttagacggagtagggcgcaaatgcttacagattatttttattatttttcggttaacctaaaaattattattgtaaataataggttatgctaaaaataaattttatcaagttacgttccaagctatactatgttcctttttgatttattgcgggaataggaccgt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02012:AA02013|AA02012:AA02013:peptide methionine sulfoxide reductase:carbonic anhydrase:->->:1369530..1369744 215 aataactccttcataatttagcataatgaaaatggtaccttttcatatacgttagacggagtagggcgcaaatgcttacagattatttttattatttttcggttaacctaaaaattattattgtaaataataggttatgctaaaaataaattttatcaagttacgttccaagctatactatgttcctttttgatttattgcgggaataggaccgt Intra-Species Hit: Count: 1 Min: 1 Max: 215 Len: 215 Subject: aact_AA02012_AA02013|peptide methionine sulfoxide reductase:carbonic anhydrase|POSITIVE:POSITIVE|[1369530,1369744]|215 HSP 1 e-value: 1.0E-118 bit: 426.0 Len: 215 Query Start:1 Query End:215 Subject Strand: POSITIVE Subject Start: 1 Subject End: 215 aataactccttcataatttagcataatgaaaatggtaccttttcatatacgttagacggagtagggcgcaaatgcttacagattatttttattatttttcggttaacctaaaaattattattgtaaataataggttatgctaaaaataaattttatcaagttacgttccaagctatactatgttcctttttgatttattgcgggaataggaccgt aataactccttcataatttagcataatgaaaatggtaccttttcatatacgttagacggagtagggcgcaaatgcttacagattatttttattatttttcggttaacctaaaaattattattgtaaataataggttatgctaaaaataaattttatcaagttacgttccaagctatactatgttcctttttgatttattgcgggaataggaccgt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAACUCCUUCAUAAUUUAGCAUAAUGAAAAUGGUACCUUUUCAUAUACGUUAGACGGAGUAGGGCGCAAAUGCUUACAGAUUAUUUUUAUUAUUUUUCGGUUAACCUAAAAAUUAUUAUUGUAAAUAAUAGGUUAUGCUAAAAAUAAAUUUUAUCAAGUUACGUUCCAAGCUAUACUAUGUUCCUUUUUGAUUUAUUGCGGGAAUAGGACCGU ....(((((.((.((((.((.....(((((((.(....)))))))).)).)))))).))))).(((((....)))))..((((((......((((((((.((((((((((....((((....))))....))))))).)))))))))))........(((..((((.............))))..)))..))))))...((((........)))) (-35.42) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACGGUCCUAUUCCCGCAAUAAAUCAAAAAGGAACAUAGUAUAGCUUGGAACGUAACUUGAUAAAAUUUAUUUUUAGCAUAACCUAUUAUUUACAAUAAUAAUUUUUAGGUUAACCGAAAAAUAAUAAAAAUAAUCUGUAAGCAUUUGCGCCCUACUCCGUCUAACGUAUAUGAAAAGGUACCAUUUUCAUUAUGCUAAAUUAUGAAGGAGUUAUU ....((((....((...............))..((((((.((((.....((((.....(((.....((((((((....((((((((((((......)))))....)))))))....))))))))............(((.((......))..)))...)))..))))..((((((((....).)))))))...)))).)))))).))))...... (-33.06) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 279 83 50 177 139 aact:AA02195|5end_oxaloacetate_decarboxylase_alpha_chain_1494501..1494731_POSITIVE 279 100 55 75 15 aact:AA01467|5end_mannose-specific_phosphotransferase_system_IID_986301..986531_POSITIVE 274 82 57 185 158 aact:AA02715|5end_paraquat-inducible_protein_pqiB_homolog_1897053..1897283_POSITIVE 272 210 165 231 191 aact:AA02090|5end_glucose-1-phosphate_adenylyltransferase_1423162..1423392_POSITIVE 270 81 57 63 37 aact:AA02674|5end_formate_dehydrogenase-related_protein_1859960..1860190_POSITIVE 263 88 54 113 82 aact:AA02895|5end_oligopeptide_transport_system_permease_protein,_C-terminal_2027484..2027714_POSITIVE 259 86 41 231 172 aact:AA00667|5end_cytolethal_distending_toxin_protein_C_457943..458173_POSITIVE 258 75 31 133 97 aact:AA01464|5end_hypothetical_protein_984210..984440_POSITIVE 254 70 21 49 4 aact:AA02762|5end_ribonuclease_H_1933164..1933394_POSITIVE 245 71 38 213 185 aact:AA02202|5end_transferrin-binding_protein_1_precursor_1499453..1499683_POSITIVE 244 210 169 123 78 aact:AA01758|5end_hydrogenase_isoenzymes_nickel_incorporation_protein_1187839..1188069_POSITIVE 241 67 21 86 41 aact:AA02592|5end_conserved_hypothetical_protein_1803031..1803261_POSITIVE 241 79 35 219 168 aact:AA01992|5end_protease_IV;_signal_peptide_peptidase;_Endopeptidase_IV_1353029..1353259_POSITIVE 240 83 51 37 3 aact:AA00781|5end_hypothetical_protein_537887..538117_POSITIVE 236 75 31 68 32 aact:AA01957|5end_conserved_hypothetical_protein_1323211..1323441_POSITIVE 236 70 21 204 152 aact:AA00675|5end_glutaredoxin_2_462524..462754_POSITIVE 235 83 44 92 47 aact:AA02472|5end_conserved_hypothetical_protein_1707631..1707861_POSITIVE 235 80 37 70 30 aact:AA01741|5end_6-pyruvoyl_tetrahydrobiopterin_synthase_1177193..1177423_POSITIVE 234 210 161 133 76 aact:AA02440|5end_ABC_transporter,_ATP-binding_protein_1685043..1685273_POSITIVE 234 67 21 66 15 aact:AA00991|5end_conserved_hypothetical_protein_693191..693421_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 265 100 58 56 3 aact:AA01466|3end_mannose-specific_phosphotransferase_system_IIC_986362..986512_POSITIVE 263 88 54 93 62 aact:AA02893|3end_oligopeptide_transport_system_permease_protein,_N-terminal_2027544..2027694_POSITIVE 258 75 31 43 7 aact:AA01463|3end_probable_transport_protein_984200..984350_POSITIVE 241 79 35 113 62 aact:AA01991|3end_conserved_hypothetical_protein_(possible_nitroreductase)_1353003..1353153_POSITIVE 241 110 65 91 40 aact:AA00280|3end_adenosylmethionine-8-amino-7-oxononanoate;_7,8-diamino-perlargonic_acid_ATase_197796..197946_POSITIVE 237 75 50 151 120 aact:AA02193|3end_oxaloacetate_decarboxylase_gamma_chain_1494562..1494712_POSITIVE 236 75 31 63 27 aact:AA01956|3end_3-deoxy-manno-octulosonate_cytidylyltransferase_1323286..1323436_POSITIVE 235 83 44 75 30 aact:AA02470|3end_glucose_kinase_1707694..1707844_POSITIVE 234 210 161 128 71 aact:AA02439|3end_integral_membrane_protein;_possible_ABC_transporter_permease_1685118..1685268_POSITIVE 234 67 21 60 9 aact:AA00990|3end_conserved_hypothetical_protein_693265..693415_POSITIVE 231 82 50 105 63 aact:AA01059|3end_orotate_phosphoribosyltransferase_735773..735923_POSITIVE 229 207 162 138 84 aact:AA01866|3end_thiamin_ABC_transporter,_permease_protein_1254946..1255096_POSITIVE 228 105 61 107 57 aact:AA02419|3end_hypothetical_protein_1661954..1662104_POSITIVE 226 69 33 128 93 aact:AA00798|3end_iron(III)_dicitrate_transport_system_permease_protein_547735..547885_POSITIVE 225 77 49 57 29 aact:AA01372|3end_ribonuclease_r,_virulence_associated_protein_930363..930513_POSITIVE 225 81 48 138 102 aact:AA01344|3end_magnesium_and_cobalt_transport_protein_912685..912835_POSITIVE 223 82 58 126 100 aact:AA02686|3end_hydrogenase_4_component_C_1872585..1872735_POSITIVE 223 203 163 104 74 aact:AA01911|3end_hypothetical_protein_1288284..1288434_POSITIVE 223 70 21 119 72 aact:AA00170|3end_hypothetical_protein_121407..121557_POSITIVE 219 80 38 40 1 aact:AA01740|3end_conserved_hypothetical_protein_1177243..1177393_POSITIVE