Origin IGS: tgccgagtccgacgcaattcttattgttatggctagtggggcataacgttacatcttgccttataccttgtcaagaaattttagccgtctaaacatctggaagtt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aacttccagatgtttagacggctaaaatttcttgacaaggtataaggcaagatgtaacgttatgccccactagccataacaataagaattgcgtcggactcggca Mask Tandem Repeat Region ================================================ tgccgagtccgacgcaattcttattgttatggctagtggggcataacgttacatcttgccttataccttgtcaagaaattttagccgtctaaacatctggaagtt Find is-nt database================================================ Query_seq: AA02004:AA02005|AA02004:AA02005:homoserine O-acetyltransferase:vancomycin sensitivity; SanA protein:->->:1363178..1363282 105 tgccgagtccgacgcaattcttattgttatggctagtggggcataacgttacatcttgccttataccttgtcaagaaattttagccgtctaaacatctggaagtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02004:AA02005|AA02004:AA02005:homoserine O-acetyltransferase:vancomycin sensitivity; SanA protein:->->:1363178..1363282 105 tgccgagtccgacgcaattcttattgttatggctagtggggcataacgttacatcttgccttataccttgtcaagaaattttagccgtctaaacatctggaagtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02004:AA02005|AA02004:AA02005:homoserine O-acetyltransferase:vancomycin sensitivity; SanA protein:->->:1363178..1363282 105 tgccgagtccgacgcaattcttattgttatggctagtggggcataacgttacatcttgccttataccttgtcaagaaattttagccgtctaaacatctggaagtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgccgagtccgacgcaattcttattgttatggctagtggggcataacgttacatcttgccttataccttgtcaagaaattttagccgtctaaacatctggaagtt Predict ORF larger than 30AA ================================================ tgccgagtccgacgcaattcttattgttatggctagtggggcataacgttacatcttgccttataccttgtcaagaaattttagccgtctaaacatctggaagtt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgccgagtccgacgcaattcttattgttatggctagtggggcataacgttacatcttgccttataccttgtcaagaaattttagccgtctaaacatctggaagtt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02004:AA02005|AA02004:AA02005:homoserine O-acetyltransferase:vancomycin sensitivity; SanA protein:->->:1363178..1363282 105 tgccgagtccgacgcaattcttattgttatggctagtggggcataacgttacatcttgccttataccttgtcaagaaattttagccgtctaaacatctggaagtt Intra-Species Hit: Count: 1 Min: 1 Max: 105 Len: 105 Subject: aact_AA02004_AA02005|homoserine O-acetyltransferase:vancomycin sensitivity; SanA protein|POSITIVE:POSITIVE|[1363178,1363282]|105 HSP 1 e-value: 3.0E-53 bit: 208.0 Len: 105 Query Start:1 Query End:105 Subject Strand: POSITIVE Subject Start: 1 Subject End: 105 tgccgagtccgacgcaattcttattgttatggctagtggggcataacgttacatcttgccttataccttgtcaagaaattttagccgtctaaacatctggaagtt tgccgagtccgacgcaattcttattgttatggctagtggggcataacgttacatcttgccttataccttgtcaagaaattttagccgtctaaacatctggaagtt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGCCGAGUCCGACGCAAUUCUUAUUGUUAUGGCUAGUGGGGCAUAACGUUACAUCUUGCCUUAUACCUUGUCAAGAAAUUUUAGCCGUCUAAACAUCUGGAAGUU (((((....))..))).((((...(((((((((((((((((((.............)))))))).................)))))))...))))...))))... (-20.54) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACUUCCAGAUGUUUAGACGGCUAAAAUUUCUUGACAAGGUAUAAGGCAAGAUGUAACGUUAUGCCCCACUAGCCAUAACAAUAAGAAUUGCGUCGGACUCGGCA ....(((.((((.......(((((......(((....))).....((((.((((...)))).))))....)))))....((((....)))))))))))....... (-18.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 363 42 4 154 112 aact:AA02723|5end_hypothetical_protein_1906263..1906493_POSITIVE 320 43 7 67 25 aact:AA00991|5end_conserved_hypothetical_protein_693191..693421_POSITIVE 316 60 11 202 140 aact:AA01159|5end_phosphatase_792828..793058_POSITIVE 304 42 1 179 139 aact:AA03013|5end_ribonuclease_P_protein_component_2104434..2104664_POSITIVE 303 48 1 208 162 aact:AA00936|5end_ribose_ABC_transporter,_permease_protein,_C-terminal_646289..646519_POSITIVE 297 43 2 116 74 aact:AA02758|5end_3-phosphoshikimate_1-carboxyvinyltransferase_1931802..1932032_POSITIVE 295 70 23 231 180 aact:AA02544|5end_ATP-dependent_RNA_helicase_1773317..1773547_POSITIVE 293 59 13 71 22 aact:AA01547|5end_deoxyribose-phosphate_aldolase_1036271..1036501_POSITIVE 285 38 4 221 172 aact:AA02649|5end_possible_glycosyltransferase_1845562..1845792_POSITIVE 282 49 1 68 16 aact:AA01029|5end_Na(+)-translocating_NADH-quinone_reductase_subunit_D_719523..719753_POSITIVE 273 49 2 86 34 aact:AA02358|5end_homoserine_kinase_1617128..1617358_POSITIVE 273 42 2 218 173 aact:AA01891|5end_pyridoxine_kinase_1275922..1276152_POSITIVE 271 59 12 225 172 aact:AA01202|5end_integrase/recombinase_823240..823470_POSITIVE 270 50 1 164 110 aact:AA01892|5end_hypothetical_protein_1276688..1276918_POSITIVE 270 73 31 186 142 aact:AA01197|5end_conserved_hypothetical_protein_820863..821093_POSITIVE 270 41 1 210 166 aact:AA01169|5end_hypothetical_protein_801985..802215_POSITIVE 270 50 2 208 173 aact:AA00870|5end_rough_colony_protein_C_601735..601965_POSITIVE 269 59 13 48 5 aact:AA02123|5end_hypothetical_protein_1448244..1448474_POSITIVE 269 48 1 71 27 aact:AA00165|5end_conserved_hypothetical_protein_117358..117588_POSITIVE 268 70 23 55 17 aact:AA02099|5end_50S_ribosomal_protein_L25_1429639..1429869_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 320 43 7 61 19 aact:AA00990|3end_conserved_hypothetical_protein_693265..693415_POSITIVE 291 43 4 42 6 aact:AA02757|3end_histidinol-phosphate_aminotransferase_2_1931808..1931958_POSITIVE 282 49 1 61 9 aact:AA01028|3end_Na(+)-translocating_NADH-quinone_reductase_subunit_E_719596..719746_POSITIVE 279 68 26 133 78 aact:AA00980|3end_hypothetical_protein_685409..685559_POSITIVE 273 49 2 70 18 aact:AA02357|3end_bifunctional_protein:_aspartokinase_I;_homoserine_dehydrogenaseI_1617192..1617342_POSITIVE 269 48 1 78 34 aact:AA00164|3end_dephospho-CoA_kinase_(dephosphocoenzyme_A_kinase)_117445..117595_POSITIVE 265 48 1 61 6 aact:AA02688|3end_hydrogenase_4_component_E_1874692..1874842_POSITIVE 262 60 12 150 104 aact:AA02195|3end_oxaloacetate_decarboxylase_alpha_chain_1496374..1496524_POSITIVE 262 48 3 102 47 aact:AA01389|3end_hypothetical_protein_937158..937308_POSITIVE 260 46 3 91 51 aact:AA01059|3end_orotate_phosphoribosyltransferase_735773..735923_POSITIVE 259 50 1 70 2 aact:AA01709|3end_glutathione-S-transferase-nitroreductase_A_fusion_protein;_modulator_of_drug_activity_A_1159334..1159484_POSITIVE 256 48 1 48 6 aact:AA02844|3end_Holliday_junction_DNA_helicase_1997885..1998035_POSITIVE 255 49 9 61 17 aact:AA00288|3end_conserved_hypothetical_protein_201347..201497_POSITIVE 254 34 4 151 121 aact:AA02722|3end_cytidine_deaminase_1906352..1906502_POSITIVE 253 71 32 148 110 aact:AA01959|3end_tetraacyldisaccharide_4'-kinase_1324463..1324613_POSITIVE 252 60 30 44 6 aact:AA01416|3end_2C-methyl-D-erythritol_2,4-cyclodiphosphate_953336..953486_POSITIVE 252 63 32 37 1 aact:AA00050|3end_cytochrome_c-type_biogenesis_protein_precursor_(reductase)_40919..41069_POSITIVE 251 46 2 67 17 aact:AA02252|3end_conserved_hypothetical_protein_1534696..1534846_POSITIVE 250 49 1 72 27 aact:AA01053|3end_conserved_hypothetical_protein_733747..733897_POSITIVE 250 71 32 94 51 aact:AA00474|3end_cell_division_protein_Y_(GTP-binding_signal_recognition_particle)_331724..331874_POSITIVE