Origin IGS: aattttctccgtaaatattttggctattataaagattgaataggttttggttaaggagaatcgcgggaaatccgatgaaaaattacacttttttacatttggctggttttttctgtgaattttttgttagaatgcaccgcgggttactataaacaggaaaaatgaggtaaaaaaatgggtacgtttggttatgcaatgatgatggttgtgctttctctcggcttaatcctggtgttggcgtttgcgattttctaattgaaaaataacttgaaatcgaaccgcactttaatcacctaaagtgcggttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaccgcactttaggtgattaaagtgcggttcgatttcaagttatttttcaattagaaaatcgcaaacgccaacaccaggattaagccgagagaaagcacaaccatcatcattgcataaccaaacgtacccatttttttacctcatttttcctgtttatagtaacccgcggtgcattctaacaaaaaattcacagaaaaaaccagccaaatgtaaaaaagtgtaatttttcatcggatttcccgcgattctccttaaccaaaacctattcaatctttataatagccaaaatatttacggagaaaatt Mask Tandem Repeat Region ================================================ aattttctccgtaaatattttggctattataaagattgaataggttttggttaaggagaatcgcgggaaatccgatgaaaaattacacttttttacatttggctggttttttctgtgaattttttgttagaatgcaccgcgggttactataaacaggaaaaatgaggtaaaaaaatgggtacgtttggttatgcaatgatgatggttgtgctttctctcggcttaatcctggtgttggcgtttgcgattttctaattgaaaaataacttgaaatcgaaccgcactttaatcacctaaagtgcggttta Find is-nt database================================================ Query_seq: AA02003:AA02004|AA02003:AA02004:purine nucleotide synthesis repressor protein:homoserine O-acetyltransferase:->->:1361769..1362076 308 aattttctccgtaaatattttggctattataaagattgaataggttttggttaaggagaatcgcgggaaatccgatgaaaaattacacttttttacatttggctggttttttctgtgaattttttgttagaatgcaccgcgggttactataaacaggaaaaatgaggtaaaaaaatgggtacgtttggttatgcaatgatgatggttgtgctttctctcggcttaatcctggtgttggcgtttgcgattttctaattgaaaaataacttgaaatcgaaccgcactttaatcacctaaagtgcggttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02003:AA02004|AA02003:AA02004:purine nucleotide synthesis repressor protein:homoserine O-acetyltransferase:->->:1361769..1362076 308 aattttctccgtaaatattttggctattataaagattgaataggttttggttaaggagaatcgcgggaaatccgatgaaaaattacacttttttacatttggctggttttttctgtgaattttttgttagaatgcaccgcgggttactataaacaggaaaaatgaggtaaaaaaatgggtacgtttggttatgcaatgatgatggttgtgctttctctcggcttaatcctggtgttggcgtttgcgattttctaattgaaaaataacttgaaatcgaaccgcactttaatcacctaaagtgcggttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02003:AA02004|AA02003:AA02004:purine nucleotide synthesis repressor protein:homoserine O-acetyltransferase:->->:1361769..1362076 308 aattttctccgtaaatattttggctattataaagattgaataggttttggttaaggagaatcgcgggaaatccgatgaaaaattacacttttttacatttggctggttttttctgtgaattttttgttagaatgcaccgcgggttactataaacaggaaaaatgaggtaaaaaaatgggtacgtttggttatgcaatgatgatggttgtgctttctctcggcttaatcctggtgttggcgtttgcgattttctaattgaaaaataacttgaaatcgaaccgcactttaatcacctaaagtgcggttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aattttctccgtaaatattttggctattataaagattgaataggttttggttaaggagaatcgcgggaaatccgatgaaaaattacacttttttacatttggctggttttttctgtgaattttttgttagaatgcaccgcgggttactataaacaggaaaaatgaggtaaaaaaatgggtacgtttggttatgcaatgatgatggttgtgctttctctcggcttaatcctggtgttggcgtttgcgattttctaattgaaaaataacttgaaatcgaaccgcactttaatcacctaaagtgcggttta Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: + Start: 36 End: 149 ................................... M N R F W L R R I A G N P M K N Y T F L H L A G F F C E F F V R M H R G L L ............................................................................................................................................................... Protein_Len: 60 Strand: - Start: 86 End: 274 ..................................................................................... C K A P K K Q S N K T L I C R P N S Y V P F I L Y F F P Y T Q N H L S S P Q A K E R S L G P T P T Q S K R I S F Y S S M .................................. Protein_Len: 40 Strand: - Start: 1 End: 120 I K E T F I K A I I F I S Y T K T L S F R P F D S S F N C K K V N P Q N K R H M ............................................................................................................................................................................................ aattttctccgtaaatattttggctattataaagattgaataggttttggttaaggagaatcgcgggaaatccgatgaaaaattacacttttttacatttggctggttttttctgtgaattttttgttagaatgcaccgcgggttactataaacaggaaaaatgaggtaaaaaaatgggtacgtttggttatgcaatgatgatggttgtgctttctctcggcttaatcctggtgttggcgtttgcgattttctaattgaaaaataacttgaaatcgaaccgcactttaatcacctaaagtgcggttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 85 Start: 26 End: 55 .........................CTTAACCAAAACCTATTCAATCTTTATAAT............................................................................................................................................................................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aattttctccgtaaatattttggctattataaagattgaataggttttggttaaggagaatcgcgggaaatccgatgaaaaattacacttttttacatttggctggttttttctgtgaattttttgttagaatgcaccgcgggttactataaacaggaaaaatgaggtaaaaaaatgggtacgtttggttatgcaatgatgatggttgtgctttctctcggcttaatcctggtgttggcgtttgcgattttctaattgaaaaataacttgaaatcgaaccgcactttaatcacctaaagtgcggttta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02003:AA02004|AA02003:AA02004:purine nucleotide synthesis repressor protein:homoserine O-acetyltransferase:->->:1361769..1362076 308 aattttctccgtaaatattttggctattataaagattgaataggttttggttaaggagaatcgcgggaaatccgatgaaaaattacacttttttacatttggctggttttttctgtgaattttttgttagaatgcaccgcgggttactataaacaggaaaaatgaggtaaaaaaatgggtacgtttggttatgcaatgatgatggttgtgctttctctcggcttaatcctggtgttggcgtttgcgattttctaattgaaaaataacttgaaatcgaaccgcactttaatcacctaaagtgcggttta Intra-Species Hit: Count: 1 Min: 1 Max: 308 Len: 308 Subject: aact_AA02003_AA02004|purine nucleotide synthesis repressor protein:homoserine O-acetyltransferase|POSITIVE:POSITIVE|[1361769, 1362076]|308 HSP 1 e-value: 8.0E-87 bit: 321.0 Len: 183 Query Start:126 Query End:308 Subject Strand: POSITIVE Subject Start: 126 Subject End: 308 .............................................................................................................................gttagaatgcaccgcgggttactataaacaggaaaaatgaggtnnnnnnntgggtacgtttggttatgcaatgatgatggttgtgctttctctcggcttaatcctggtgttggcgtttgcgattttctaattgaaaaataacttgaaatcgaaccgcactttaatcacctaaagtgcggttta .............................................................................................................................gttagaatgcaccgcgggttactataaacaggaaaaatgaggtaaaaaaatgggtacgtttggttatgcaatgatgatggttgtgctttctctcggcttaatcctggtgttggcgtttgcgattttctaattgaaaaataacttgaaatcgaaccgcactttaatcacctaaagtgcggttta HSP 2 e-value: 1.0E-42 bit: 174.0 Len: 88 Query Start:1 Query End:88 Subject Strand: POSITIVE Subject Start: 1 Subject End: 88 aattttctccgtaaatattttggctattataaagattgaataggttttggttaaggagaatcgcgggaaatccgatgaaaaattacac............................................................................................................................................................................................................................ aattttctccgtaaatattttggctattataaagattgaataggttttggttaaggagaatcgcgggaaatccgatgaaaaattacac............................................................................................................................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUUUUCUCCGUAAAUAUUUUGGCUAUUAUAAAGAUUGAAUAGGUUUUGGUUAAGGAGAAUCGCGGGAAAUCCGAUGAAAAAUUACACUUUUUUACAUUUGGCUGGUUUUUUCUGUGAAUUUUUUGUUAGAAUGCACCGCGGGUUACUAUAAACAGGAAAAAUGAGGUAAAAAAAUGGGUACGUUUGGUUAUGCAAUGAUGAUGGUUGUGCUUUCUCUCGGCUUAAUCCUGGUGUUGGCGUUUGCGAUUUUCUAAUUGAAAAAUAACUUGAAAUCGAACCGCACUUUAAUCACCUAAAGUGCGGUUUA ..(((((.(((.((((..((..(((.......)).)..))...)))))))....(((((((((((((...((..(..(((((((.(((.......((......)).........))))))))))..)..))..((((((.((((((....................((((..(((((....)))))..))))((...((.((.((.....)).))))...)).)))))))))))).....)))))))))))))....))))).............(((((((((((((......))))))))))))). (-71.59) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACCGCACUUUAGGUGAUUAAAGUGCGGUUCGAUUUCAAGUUAUUUUUCAAUUAGAAAAUCGCAAACGCCAACACCAGGAUUAAGCCGAGAGAAAGCACAACCAUCAUCAUUGCAUAACCAAACGUACCCAUUUUUUUACCUCAUUUUUCCUGUUUAUAGUAACCCGCGGUGCAUUCUAACAAAAAAUUCACAGAAAAAACCAGCCAAAUGUAAAAAAGUGUAAUUUUUCAUCGGAUUUCCCGCGAUUCUCCUUAACCAAAACCUAUUCAAUCUUUAUAAUAGCCAAAAUAUUUACGGAGAAAAUU ..(((((((((((((....)))))))))))))(((((((.((((.......)))).).))))))....(((........(((.....(((((((((((((.............)))...............(((((((((((............(((......)))..(((((...((((..............))))...))).)).....)))))))))))...)))))).))))...))).))).((((((.(((.......(((((..........))))).........))).)))))).... (-54.45) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 387 246 201 225 186 aact:AA02763|5end_DNA_polymerase_III,_epsilon_subunit_1933696..1933926_POSITIVE 344 260 217 193 141 aact:AA02002|5end_hypothetical_protein_1360196..1360426_POSITIVE 344 258 219 73 29 aact:AA00749|5end_galactose_operon_repressor_(gal_repressor)_513781..514011_POSITIVE 340 246 203 173 135 aact:AA00528|5end_conserved_hypothetical_protein_361396..361626_POSITIVE 334 220 177 156 104 aact:AA00959|5end_nitrate/nitrite_response_regulator_664596..664826_POSITIVE 322 240 193 73 31 aact:AA02470|5end_glucose_kinase_1706730..1706960_POSITIVE 322 247 205 198 158 aact:AA00892|5end_selenocysteine-specific_elongation_factor_(SelB_translation_factor)_618023..618253_POSITIVE 321 248 201 81 35 aact:AA00370|5end_30S_ribosomal_protein_S10_248620..248850_POSITIVE 320 260 216 111 58 aact:AA01085|5end_mutator_mutT_protein_(7,8-dihydro-8-oxoguanine-triphosphatase)_(8-oxo-dGTPase)_(dGTP_pyrophosphohydrolase)_750970..751200_POSITIVE 315 229 181 219 172 aact:AA00160|5end_pilA-like_biogenesis_protein_/_prepilin_peptidase_dependent_proteinD_112836..113066_POSITIVE 311 250 203 153 103 aact:AA02359|5end_hypothetical_protein_1618100..1618330_POSITIVE 311 246 205 173 117 aact:AA00521|5end_phosphatase_357744..357974_POSITIVE 311 246 201 124 71 aact:AA00333|5end_1-phosphofructokinase;_tagatose-6-phosphate_kinase_225543..225773_POSITIVE 310 239 193 181 143 aact:AA01192|5end_conserved_hypothetical_protein_(possible_membrane_protein)_817358..817588_POSITIVE 309 240 193 146 88 aact:AA02565|5end_transketolase,_N-terminal_subunit_1786366..1786596_POSITIVE 309 246 206 147 93 aact:AA01140|5end_anaerobic_dimethyl_sulfoxide_reductase,_chain_C_781610..781840_POSITIVE 308 248 202 88 43 aact:AA00643|5end_hypothetical_protein_440432..440662_POSITIVE 306 244 203 165 123 aact:AA01260|5end_hypothetical_protein_855550..855780_POSITIVE 304 240 191 227 167 aact:AA01589|5end_conserved_hypothetical_protein_1070300..1070530_POSITIVE 300 246 201 140 79 aact:AA02140|5end_ABC_transporter_ATP-binding_protein_1462144..1462374_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 381 240 201 151 118 aact:AA02762|3end_ribonuclease_H_1933708..1933858_POSITIVE 361 247 206 84 29 aact:AA01031|3end_Na(+)-translocating_NADH-quinone_reductase_subunit_C_721077..721227_POSITIVE 334 220 177 143 91 aact:AA00958|3end_adenylate_cyclase_664663..664813_POSITIVE 324 258 228 38 5 aact:AA00748|3end_hypothetical_protein_513826..513976_POSITIVE 322 240 193 60 18 aact:AA02468|3end_conserved_hypothetical_protein_1706797..1706947_POSITIVE 322 247 205 149 109 aact:AA00890|3end_conserved_hypothetical_protein_618054..618204_POSITIVE 321 248 201 61 15 aact:AA00369|3end_50S_ribosomal_protein_L3_248680..248830_POSITIVE 312 252 217 151 104 aact:AA02001|3end_conserved_hypothetical_protein_1360239..1360389_POSITIVE 311 250 203 122 72 aact:AA02358|3end_homoserine_kinase_1618149..1618299_POSITIVE 311 246 201 118 65 aact:AA00332|3end_PTS_system,_fructose-specific_IIA/FPr_component_(diphosphoryl_transfer_protein)_225617..225767_POSITIVE 309 246 206 142 88 aact:AA01138|3end_anaerobic_dimethyl_sulfoxide_reductase,_chain_B_781685..781835_POSITIVE 308 247 201 145 90 aact:AA01262|3end_hydrolase_860087..860237_POSITIVE 303 239 204 101 73 aact:AA01565|3end_conserved_hypothetical_protein_1050967..1051117_POSITIVE 300 246 201 134 73 aact:AA02139|3end_possible_membrane_protein_1462218..1462368_POSITIVE 299 242 208 143 104 aact:AA01818|3end_high-affinity_zinc_uptake_system_membrane_protein_1225078..1225228_POSITIVE 296 229 186 151 111 aact:AA02695|3end_hydrogenase-3_component_G_1879418..1879568_POSITIVE 295 245 201 88 35 aact:AA00528|3end_conserved_hypothetical_protein_361703..361853_POSITIVE 294 240 191 89 40 aact:AA00380|3end_carbamate_kinase_257537..257687_POSITIVE 292 239 196 134 85 aact:AA01192|3end_conserved_hypothetical_protein_(possible_membrane_protein)_817977..818127_POSITIVE 288 296 259 149 112 aact:AA01978|3end_hypothetical_protein_1337257..1337407_POSITIVE