Origin IGS: tgtgtataccgtacactattaagggttaatagcaatcgtataatggttcgcaacgtttgttgttgcaggcgatgatgtcttttttgtccgcatgcggagttgtcagtgaatcgtaatagtagaagggacggagagtttactgtgaagttatttttctactatgcaaaggataatagccatatttagtgcggtcatttttatatgtgtttttaaataattataaaatgtttaataactattaaaaatgatgatgtgtgaatatagaaaaaaggcatgtgaatgacgccctcatgcggtttgtctta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taagacaaaccgcatgagggcgtcattcacatgccttttttctatattcacacatcatcatttttaatagttattaaacattttataattatttaaaaacacatataaaaatgaccgcactaaatatggctattatcctttgcatagtagaaaaataacttcacagtaaactctccgtcccttctactattacgattcactgacaactccgcatgcggacaaaaaagacatcatcgcctgcaacaacaaacgttgcgaaccattatacgattgctattaacccttaatagtgtacggtatacaca Mask Tandem Repeat Region ================================================ tgtgtataccgtacactattaagggttaatagcaatcgtataatggttcgcaacgtttgttgttgcaggcgatgatgtcttttttgtccgcatgcggagttgtcagtgaatcgtaatagtagaagggacggagagtttactgtgaagttatttttctactatgcaaaggataatagccatatttagtgcggtcatttttatatgtgtttttaaataattataaaatgtttaataactattaaaaatgatgatgtgtgaatatagaaaaaaggcatgtgaatgacgccctcatgcggtttgtctta Find is-nt database================================================ Query_seq: AA02002:AA02003|AA02002:AA02003:hypothetical protein:purine nucleotide synthesis repressor protein:->->:1360468..1360772 305 tgtgtataccgtacactattaagggttaatagcaatcgtataatggttcgcaacgtttgttgttgcaggcgatgatgtcttttttgtccgcatgcggagttgtcagtgaatcgtaatagtagaagggacggagagtttactgtgaagttatttttctactatgcaaaggataatagccatatttagtgcggtcatttttatatgtgtttttaaataattataaaatgtttaataactattaaaaatgatgatgtgtgaatatagaaaaaaggcatgtgaatgacgccctcatgcggtttgtctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02002:AA02003|AA02002:AA02003:hypothetical protein:purine nucleotide synthesis repressor protein:->->:1360468..1360772 305 tgtgtataccgtacactattaagggttaatagcaatcgtataatggttcgcaacgtttgttgttgcaggcgatgatgtcttttttgtccgcatgcggagttgtcagtgaatcgtaatagtagaagggacggagagtttactgtgaagttatttttctactatgcaaaggataatagccatatttagtgcggtcatttttatatgtgtttttaaataattataaaatgtttaataactattaaaaatgatgatgtgtgaatatagaaaaaaggcatgtgaatgacgccctcatgcggtttgtctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02002:AA02003|AA02002:AA02003:hypothetical protein:purine nucleotide synthesis repressor protein:->->:1360468..1360772 305 tgtgtataccgtacactattaagggttaatagcaatcgtataatggttcgcaacgtttgttgttgcaggcgatgatgtcttttttgtccgcatgcggagttgtcagtgaatcgtaatagtagaagggacggagagtttactgtgaagttatttttctactatgcaaaggataatagccatatttagtgcggtcatttttatatgtgtttttaaataattataaaatgtttaataactattaaaaatgatgatgtgtgaatatagaaaaaaggcatgtgaatgacgccctcatgcggtttgtctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgtgtataccgtacactattaagggttaatagcaatcgtataatggttcgcaacgtttgttgttgcaggcgatgatgtcttttttgtccgcatgcggagttgtcagtgaatcgtaatagtagaagggacggagagtttactgtgaagttatttttctactatgcaaaggataatagccatatttagtgcggtcatttttatatgtgtttttaaataattataaaatgtttaataactattaaaaatgatgatgtgtgaatatagaaaaaaggcatgtgaatgacgccctcatgcggtttgtctta Predict ORF larger than 30AA ================================================ tgtgtataccgtacactattaagggttaatagcaatcgtataatggttcgcaacgtttgttgttgcaggcgatgatgtcttttttgtccgcatgcggagttgtcagtgaatcgtaatagtagaagggacggagagtttactgtgaagttatttttctactatgcaaaggataatagccatatttagtgcggtcatttttatatgtgtttttaaataattataaaatgtttaataactattaaaaatgatgatgtgtgaatatagaaaaaaggcatgtgaatgacgccctcatgcggtttgtctta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 82 Start: 217 End: 246 ........................................................................................................................................................................................................................ATTTTTAATAGTTATTAAACATTTTATAAT........................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgtgtataccgtacactattaagggttaatagcaatcgtataatggttcgcaacgtttgttgttgcaggcgatgatgtcttttttgtccgcatgcggagttgtcagtgaatcgtaatagtagaagggacggagagtttactgtgaagttatttttctactatgcaaaggataatagccatatttagtgcggtcatttttatatgtgtttttaaataattataaaatgtttaataactattaaaaatgatgatgtgtgaatatagaaaaaaggcatgtgaatgacgccctcatgcggtttgtctta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02002:AA02003|AA02002:AA02003:hypothetical protein:purine nucleotide synthesis repressor protein:->->:1360468..1360772 305 tgtgtataccgtacactattaagggttaatagcaatcgtataatggttcgcaacgtttgttgttgcaggcgatgatgtcttttttgtccgcatgcggagttgtcagtgaatcgtaatagtagaagggacggagagtttactgtgaagttatttttctactatgcaaaggataatagccatatttagtgcggtcatttttatatgtgtttttaaataattataaaatgtttaataactattaaaaatgatgatgtgtgaatatagaaaaaaggcatgtgaatgacgccctcatgcggtttgtctta Intra-Species Hit: Count: 1 Min: 1 Max: 305 Len: 305 Subject: aact_AA02002_AA02003|hypothetical protein:purine nucleotide synthesis repressor protein|POSITIVE:POSITIVE|[1360468,1360772]|305 HSP 1 e-value: 1.0E-172 bit: 605.0 Len: 305 Query Start:1 Query End:305 Subject Strand: POSITIVE Subject Start: 1 Subject End: 305 tgtgtataccgtacactattaagggttaatagcaatcgtataatggttcgcaacgtttgttgttgcaggcgatgatgtcttttttgtccgcatgcggagttgtcagtgaatcgtaatagtagaagggacggagagtttactgtgaagttatttttctactatgcaaaggataatagccatatttagtgcggtcatttttatatgtgtttttaaataattataaaatgtttaataactattaaaaatgatgatgtgtgaatatagaaaaaaggcatgtgaatgacgccctcatgcggtttgtctta tgtgtataccgtacactattaagggttaatagcaatcgtataatggttcgcaacgtttgttgttgcaggcgatgatgtcttttttgtccgcatgcggagttgtcagtgaatcgtaatagtagaagggacggagagtttactgtgaagttatttttctactatgcaaaggataatagccatatttagtgcggtcatttttatatgtgtttttaaataattataaaatgtttaataactattaaaaatgatgatgtgtgaatatagaaaaaaggcatgtgaatgacgccctcatgcggtttgtctta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUGUAUACCGUACACUAUUAAGGGUUAAUAGCAAUCGUAUAAUGGUUCGCAACGUUUGUUGUUGCAGGCGAUGAUGUCUUUUUUGUCCGCAUGCGGAGUUGUCAGUGAAUCGUAAUAGUAGAAGGGACGGAGAGUUUACUGUGAAGUUAUUUUUCUACUAUGCAAAGGAUAAUAGCCAUAUUUAGUGCGGUCAUUUUUAUAUGUGUUUUUAAAUAAUUAUAAAAUGUUUAAUAACUAUUAAAAAUGAUGAUGUGUGAAUAUAGAAAAAAGGCAUGUGAAUGACGCCCUCAUGCGGUUUGUCUUA .((((((...))))))...(((((.......((((.(((...((.((..((((((.....))))))..)).)).)))......))))(((((((.((.((.((((.......(((.(((((((((.(((..(.((....)).)...)))...))))))))))))...........((((((((((.((((((((((((((.(((.(((.(((((((.........))))))).))))))))))))))))..)))))))))))........))).......)))))))).)))))))....))))) (-70.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGACAAACCGCAUGAGGGCGUCAUUCACAUGCCUUUUUUCUAUAUUCACACAUCAUCAUUUUUAAUAGUUAUUAAACAUUUUAUAAUUAUUUAAAAACACAUAUAAAAAUGACCGCACUAAAUAUGGCUAUUAUCCUUUGCAUAGUAGAAAAAUAACUUCACAGUAAACUCUCCGUCCCUUCUACUAUUACGAUUCACUGACAACUCCGCAUGCGGACAAAAAAGACAUCAUCGCCUGCAACAACAAACGUUGCGAACCAUUAUACGAUUGCUAUUAACCCUUAAUAGUGUACGGUAUACACA .........(((((((.(((.((((................(((((((.........(((((((((((((((((..........)))))))).............)))))))))........)))))))..............(((((((((.....(((....)))..((.....))...)))))))))..........))))...))).))))))).................(((.(((((.......)))))................(((((((.....)))))))....)))....... (-48.84) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 352 108 61 186 134 aact:AA01203|5end_diaminopimelate_epimerase_824140..824370_POSITIVE 350 75 31 62 15 aact:AA01983|5end_maltose_regulon_positive_regulatory_protein_1341947..1342177_POSITIVE 349 97 59 143 113 aact:AA01108|5end_hypothetical_protein_763646..763876_POSITIVE 349 210 174 63 30 aact:AA00582|5end_conserved_hypothetical_protein_397904..398134_POSITIVE 348 98 54 141 93 aact:AA00424|5end_hypothetical_protein_287123..287353_POSITIVE 340 99 54 230 170 aact:AA00719|5end_hypothetical_protein_494627..494857_POSITIVE 337 120 77 97 44 aact:AA01063|5end_conserved_hypothetical_protein_736537..736767_POSITIVE 335 97 54 139 95 aact:AA00500|5end_hypothetical_protein_347151..347381_POSITIVE 334 108 63 190 135 aact:AA01206|5end_phosphoribosylformylglycinamidine_cyclo-ligase_825222..825452_POSITIVE 330 79 43 153 122 aact:AA02743|5end_outer_membrane_lipoprotein_precursor/glutamyl-tRNA_reductase_subunit_1922650..1922880_POSITIVE 330 110 61 83 44 aact:AA01224|5end_CTP_synthase_836221..836451_POSITIVE 330 97 54 225 181 aact:AA01085|5end_mutator_mutT_protein_(7,8-dihydro-8-oxoguanine-triphosphatase)_(8-oxo-dGTPase)_(dGTP_pyrophosphohydrolase)_750970..751200_POSITIVE 325 97 52 61 15 aact:AA00422|5end_LapB_membrane_protein_285528..285758_POSITIVE 320 80 36 80 36 aact:AA02402|5end_O-sialoglycoprotein_endopeptidase_1651337..1651567_POSITIVE 320 73 31 49 16 aact:AA01166|5end_sulphite_synthesis_pathway_protein_798599..798829_POSITIVE 319 205 161 208 166 aact:AA02936|5end_lipoprotein_E_precursor;_outer_membrane_protein_P4_precursor;_acid_phosphatase_2055610..2055840_POSITIVE 319 90 43 102 45 aact:AA02563|5end_transketolase_C-terminal_section_1785239..1785469_POSITIVE 319 103 66 41 1 aact:AA01966|5end_2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_pyrophosphokinase_1330937..1331167_POSITIVE 318 108 68 204 168 aact:AA01794|5end_glycine_cleavage_system_transcription_activator_1209708..1209938_POSITIVE 314 67 21 134 83 aact:AA01792|5end_conserved_hypothetical_protein_1208058..1208288_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 349 210 174 45 12 aact:AA00581|3end_thiosulfate_sulfurtransferase_(rhodanese)_397966..398116_POSITIVE 334 108 63 84 29 aact:AA01204|3end_hypothetical_protein_825196..825346_POSITIVE 330 79 43 121 90 aact:AA02742|3end_tRNA_nucleotidyltransferase_1922698..1922848_POSITIVE 330 97 54 145 101 aact:AA01083|3end_preprotein_translocase_SecA_subunit_750970..751120_POSITIVE 325 113 73 151 109 aact:AA00377|3end_acetyl-CoA_acetyltransferase_(Beta-ketothiolase,3-ketoacyl-CoA_thiolase)_254300..254450_POSITIVE 320 80 36 54 10 aact:AA02400|3end_hypothetical_protein_1651391..1651541_POSITIVE 319 103 66 45 5 aact:AA01965|3end_poly(A)_polymerase_1331021..1331171_POSITIVE 314 67 21 131 80 aact:AA01790|3end_riboflavin_bifunctional_biosynthesis_protein_1208135..1208285_POSITIVE 312 137 94 145 98 aact:AA00988|3end_conserved_hypothetical_protein_692264..692414_POSITIVE 306 98 51 64 21 aact:AA01201|3end_phosphatase;_hydrolase_823303..823453_POSITIVE 296 90 44 72 25 aact:AA01183|3end_conserved_hypothetical_protein_810902..811052_POSITIVE 296 97 53 83 47 aact:AA00741|3end_possible_2-oxoglutarate/malate_transporter_508076..508226_POSITIVE 294 96 59 114 73 aact:AA02153|3end_iron(III)_ABC_transporter,_permease_protein_1473620..1473770_POSITIVE 291 215 185 46 16 aact:AA01057|3end_DnaJ-like_protein_djlA_735047..735197_POSITIVE 291 211 175 88 48 aact:AA00168|3end_possible_sigma_54_modulation_protein_120240..120390_POSITIVE 290 130 84 87 52 aact:AA01482|3end_ATP_synthase_F1,_subunit_beta_997735..997885_POSITIVE 290 109 64 94 41 aact:AA01463|3end_probable_transport_protein_984200..984350_POSITIVE 288 108 64 93 54 aact:AA00530|3end_hypothetical_protein_362668..362818_POSITIVE 287 167 124 121 74 aact:AA01961|3end_lipid_A_export_ATP-binding/permease_protein_msbA_1326230..1326380_POSITIVE 286 130 86 84 47 aact:AA00257|3end_rod-shape-determining_protein_180904..181054_POSITIVE