Origin IGS: tttcttttccaacataaaatagttgagtgtaatagtaggttaatttgctttgctatgcaatattttatgcgcattatgtgacgggtaaataaaatcgataaagtgcggtcatttttttgattactttggcaaattataccaatttgtgattatttaaaaatgaacggcaaataggatattttctggcttagcgtgaaaaaccgctatcattttttcaaaaaatctaataagattcgtattatttgttaaaaatttgtaatgtctgttttttgtacagaaattgtataagaatttttgacagattaaacatattttagttatcaattcgttgtttaatttctacaaaagtaggttaaacgtacttctaacttaacaaagataaggaaaaagct .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agctttttccttatctttgttaagttagaagtacgtttaacctacttttgtagaaattaaacaacgaattgataactaaaatatgtttaatctgtcaaaaattcttatacaatttctgtacaaaaaacagacattacaaatttttaacaaataatacgaatcttattagattttttgaaaaaatgatagcggtttttcacgctaagccagaaaatatcctatttgccgttcatttttaaataatcacaaattggtataatttgccaaagtaatcaaaaaaatgaccgcactttatcgattttatttacccgtcacataatgcgcataaaatattgcatagcaaagcaaattaacctactattacactcaactattttatgttggaaaagaaa Mask Tandem Repeat Region ================================================ tttcttttccaacataaaatagttgagtgtaatagtaggttaatttgctttgctatgcaatattttatgcgcattatgtgacgggtaaataaaatcgataaagtgcggtcatttttttgattactttggcaaattataccaatttgtgattatttaaaaatgaacggcaaataggatattttctggcttagcgtgaaaaaccgctatcattttttcaaaaaatctaataagattcgtattatttgttaaaaatttgtaatgtctgttttttgtacagaaattgtataagaatttttgacagattaaacatattttagttatcaattcgttgtttaatttctacaaaagtaggttaaacgtacttctaacttaacaaagataaggaaaaagct Find is-nt database================================================ Query_seq: AA01984:AA01985|AA01984:AA01985:tellurite resistance protein; hemagglutinin homolog:pyruvate dehydrogenase, E1 component:->->:1345815..1346206 392 tttcttttccaacataaaatagttgagtgtaatagtaggttaatttgctttgctatgcaatattttatgcgcattatgtgacgggtaaataaaatcgataaagtgcggtcatttttttgattactttggcaaattataccaatttgtgattatttaaaaatgaacggcaaataggatattttctggcttagcgtgaaaaaccgctatcattttttcaaaaaatctaataagattcgtattatttgttaaaaatttgtaatgtctgttttttgtacagaaattgtataagaatttttgacagattaaacatattttagttatcaattcgttgtttaatttctacaaaagtaggttaaacgtacttctaacttaacaaagataaggaaaaagct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01984:AA01985|AA01984:AA01985:tellurite resistance protein; hemagglutinin homolog:pyruvate dehydrogenase, E1 component:->->:1345815..1346206 392 tttcttttccaacataaaatagttgagtgtaatagtaggttaatttgctttgctatgcaatattttatgcgcattatgtgacgggtaaataaaatcgataaagtgcggtcatttttttgattactttggcaaattataccaatttgtgattatttaaaaatgaacggcaaataggatattttctggcttagcgtgaaaaaccgctatcattttttcaaaaaatctaataagattcgtattatttgttaaaaatttgtaatgtctgttttttgtacagaaattgtataagaatttttgacagattaaacatattttagttatcaattcgttgtttaatttctacaaaagtaggttaaacgtacttctaacttaacaaagataaggaaaaagct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01984:AA01985|AA01984:AA01985:tellurite resistance protein; hemagglutinin homolog:pyruvate dehydrogenase, E1 component:->->:1345815..1346206 392 tttcttttccaacataaaatagttgagtgtaatagtaggttaatttgctttgctatgcaatattttatgcgcattatgtgacgggtaaataaaatcgataaagtgcggtcatttttttgattactttggcaaattataccaatttgtgattatttaaaaatgaacggcaaataggatattttctggcttagcgtgaaaaaccgctatcattttttcaaaaaatctaataagattcgtattatttgttaaaaatttgtaatgtctgttttttgtacagaaattgtataagaatttttgacagattaaacatattttagttatcaattcgttgtttaatttctacaaaagtaggttaaacgtacttctaacttaacaaagataaggaaaaagct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tttcttttccaacataaaatagttgagtgtaatagtaggttaatttgctttgctatgcaatattttatgcgcattatgtgacgggtaaataaaatcgataaagtgcggtcatttttttgattactttggcaaattataccaatttgtgattatttaaaaatgaacggcaaataggatattttctggcttagcgtgaaaaaccgctatcattttttcaaaaaatctaataagattcgtattatttgttaaaaatttgtaatgtctgttttttgtacagaaattgtataagaatttttgacagattaaacatattttagttatcaattcgttgtttaatttctacaaaagtaggttaaacgtacttctaacttaacaaagataaggaaaaagct Predict ORF larger than 30AA ================================================ Protein_Len: 56 Strand: - Start: 157 End: 324 ............................................................................................................................................................ F H V A F L I N E P K A H F V A I M K E F F R I L N T N N T L F K Y H R N K T C F N Y L F K Q C I L C I K T I M .................................................................... Protein_Len: 47 Strand: - Start: 3 End: 143 .. R K W C L I T S H L L L N I Q K A I C Y K I R M I H R T F L I S L T R D N K Q N S Q C I I G M ......................................................................................................................................................................................................................................................... tttcttttccaacataaaatagttgagtgtaatagtaggttaatttgctttgctatgcaatattttatgcgcattatgtgacgggtaaataaaatcgataaagtgcggtcatttttttgattactttggcaaattataccaatttgtgattatttaaaaatgaacggcaaataggatattttctggcttagcgtgaaaaaccgctatcattttttcaaaaaatctaataagattcgtattatttgttaaaaatttgtaatgtctgttttttgtacagaaattgtataagaatttttgacagattaaacatattttagttatcaattcgttgtttaatttctacaaaagtaggttaaacgtacttctaacttaacaaagataaggaaaaagct Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 213 End: 242 ....................................................................................................................................................................................................................TTTCAAAAAATCTAATAAGATTCGTATTAT...................................................................................................................................................... PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 221 End: 250 ............................................................................................................................................................................................................................TTTAACAAATAATACGAATCTTATTAGATT.............................................................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tttcttttccaacataaaatagttgagtgtaatagtaggttaatttgctttgctatgcaatattttatgcgcattatgtgacgggtaaataaaatcgataaagtgcggtcatttttttgattactttggcaaattataccaatttgtgattatttaaaaatgaacggcaaataggatattttctggcttagcgtgaaaaaccgctatcattttttcaaaaaatctaataagattcgtattatttgttaaaaatttgtaatgtctgttttttgtacagaaattgtataagaatttttgacagattaaacatattttagttatcaattcgttgtttaatttctacaaaagtaggttaaacgtacttctaacttaacaaagataaggaaaaagct Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 46 HP_score: -3.8 Tail_Score: -3.02639 Start: 130 End: 146 Full_Region: TTTTGATTACTTTGG CAAATT ATACC AATTTG TGATTATTTAAAAAT .................................................................................................................................CAAATTATACCAATTTG...................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: AA01984:AA01985|AA01984:AA01985:tellurite resistance protein; hemagglutinin homolog:pyruvate dehydrogenase, E1 component:->->:1345815..1346206 392 tttcttttccaacataaaatagttgagtgtaatagtaggttaatttgctttgctatgcaatattttatgcgcattatgtgacgggtaaataaaatcgataaagtgcggtcatttttttgattactttggcaaattataccaatttgtgattatttaaaaatgaacggcaaataggatattttctggcttagcgtgaaaaaccgctatcattttttcaaaaaatctaataagattcgtattatttgttaaaaatttgtaatgtctgttttttgtacagaaattgtataagaatttttgacagattaaacatattttagttatcaattcgttgtttaatttctacaaaagtaggttaaacgtacttctaacttaacaaagataaggaaaaagct Intra-Species Hit: Count: 1 Min: 1 Max: 392 Len: 392 Subject: aact_AA01984_AA01985|tellurite resistance protein; hemagglutinin homolog:pyruvate dehydrogenase, E1 component|POSITIVE:POSITIVE|[1345815,1346206]|392 HSP 1 e-value: 0.0 bit: 735.0 Len: 392 Query Start:1 Query End:392 Subject Strand: POSITIVE Subject Start: 1 Subject End: 392 tttcttttccaacataaaatagttgagtgtaatagtaggttaatttgctttgctatgcaatattttatgcgcattatgtgacgggtaaataaaatcgataaagtgcggtcannnnnnngattactttggcaaattataccaatttgtgattatttaaaaatgaacggcaaataggatattttctggcttagcgtgaaaaaccgctatcattttttcaaaaaatctaataagattcgtattatttgttaaaaatttgtaatgtctgttttttgtacagaaattgtataagaatttttgacagattaaacatattttagttatcaattcgttgtttaatttctacaaaagtaggttaaacgtacttctaacttaacaaagataaggaaaaagct tttcttttccaacataaaatagttgagtgtaatagtaggttaatttgctttgctatgcaatattttatgcgcattatgtgacgggtaaataaaatcgataaagtgcggtcatttttttgattactttggcaaattataccaatttgtgattatttaaaaatgaacggcaaataggatattttctggcttagcgtgaaaaaccgctatcattttttcaaaaaatctaataagattcgtattatttgttaaaaatttgtaatgtctgttttttgtacagaaattgtataagaatttttgacagattaaacatattttagttatcaattcgttgtttaatttctacaaaagtaggttaaacgtacttctaacttaacaaagataaggaaaaagct Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUCUUUUCCAACAUAAAAUAGUUGAGUGUAAUAGUAGGUUAAUUUGCUUUGCUAUGCAAUAUUUUAUGCGCAUUAUGUGACGGGUAAAUAAAAUCGAUAAAGUGCGGUCAUUUUUUUGAUUACUUUGGCAAAUUAUACCAAUUUGUGAUUAUUUAAAAAUGAACGGCAAAUAGGAUAUUUUCUGGCUUAGCGUGAAAAACCGCUAUCAUUUUUUCAAAAAAUCUAAUAAGAUUCGUAUUAUUUGUUAAAAAUUUGUAAUGUCUGUUUUUUGUACAGAAAUUGUAUAAGAAUUUUUGACAGAUUAAACAUAUUUUAGUUAUCAAUUCGUUGUUUAAUUUCUACAAAAGUAGGUUAAACGUACUUCUAACUUAACAAAGAUAAGGAAAAAGCU ....((((((............(((((.((.(((((.((((....((((..((((.(.((((((((((.((((((.(((..(((..........)))))).)))))).((((((((.(((((((.((((.........))))...)))))))...)))))))).......)))))))))).).))))..)))).....))))))))).))...)))))...((((..((((((..((...(((((((((((((((.((((.(((((.......))))).)))).....)))))))))))))))...))..))))))((((.......((((((((((..((((....)))))))))))).))........))))..))))..)))))).... (-84.16) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGCUUUUUCCUUAUCUUUGUUAAGUUAGAAGUACGUUUAACCUACUUUUGUAGAAAUUAAACAACGAAUUGAUAACUAAAAUAUGUUUAAUCUGUCAAAAAUUCUUAUACAAUUUCUGUACAAAAAACAGACAUUACAAAUUUUUAACAAAUAAUACGAAUCUUAUUAGAUUUUUUGAAAAAAUGAUAGCGGUUUUUCACGCUAAGCCAGAAAAUAUCCUAUUUGCCGUUCAUUUUUAAAUAAUCACAAAUUGGUAUAAUUUGCCAAAGUAAUCAAAAAAAUGACCGCACUUUAUCGAUUUUAUUUACCCGUCACAUAAUGCGCAUAAAAUAUUGCAUAGCAAAGCAAAUUAACCUACUAUUACACUCAACUAUUUUAUGUUGGAAAAGAAA ...(((((((....((((((((.....((.(((.((((((.((((....))))...))))))...((((((((((.......((((((..(((((((((((.((......(((.(((((.......))))).)))..............(((((........))))).)).)))))))........(((((........)))))...))))))))))......(((.((.(((((((.............((((((.....)))))).(....)..))))))))).)))..))))))))))....)))...)).........(((........))).)))))))).......................(((........))))))))))... (-62.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 352 117 80 96 56 aact:AA00618|5end_molybdopterin_biosynthesis_protein_422984..423214_POSITIVE 345 116 78 95 56 aact:AA00991|5end_conserved_hypothetical_protein_693191..693421_POSITIVE 325 119 77 186 141 aact:AA03008|5end_GTP-binding_protein_era_homolog_(GTPase)_2100087..2100317_POSITIVE 324 119 78 102 58 aact:AA02335|5end_6-O-methylguanine-DNA_methyltransferase_1596814..1597044_POSITIVE 321 123 81 71 33 aact:AA01271|5end_hypothetical_protein_863794..864024_POSITIVE 319 86 44 208 169 aact:AA02693|5end_hydrogenase-4_component_H_1877921..1878151_POSITIVE 316 130 81 195 138 aact:AA01401|5end_3-deoxy-D-manno-octulosonic-acid_transferase_946165..946395_POSITIVE 315 120 71 162 120 aact:AA01436|5end_fructose-bisphosphate_aldolase_class_II_962981..963211_POSITIVE 314 110 67 109 59 aact:AA01059|5end_orotate_phosphoribosyltransferase_735052..735282_POSITIVE 311 98 51 98 51 aact:AA01464|5end_hypothetical_protein_984210..984440_POSITIVE 309 130 81 127 76 aact:AA01872|5end_conserved_hypothetical_protein_1258804..1259034_POSITIVE 308 140 94 165 120 aact:AA01171|5end_LysA_activator_protein_802811..803041_POSITIVE 304 209 166 63 11 aact:AA02179|5end_anthanilate_phosphoribosyltransferase_1489367..1489597_POSITIVE 304 110 67 147 110 aact:AA00613|5end_lipid-A-disaccharide_synthase_418713..418943_POSITIVE 303 130 99 181 148 aact:AA02716|5end_peptidase_T_1899789..1900019_POSITIVE 295 119 71 214 158 aact:AA01276|5end_heptosyltransferase_III_864730..864960_POSITIVE 293 146 101 179 130 aact:AA01559|5end_RecA_regulatory_protein,_RecX_1045970..1046200_POSITIVE 293 140 96 193 146 aact:AA00485|5end_hypothetical_protein_336494..336724_POSITIVE 293 200 155 166 108 aact:AA00420|5end_tRNA/rRNA_methyltransferase_284953..285183_POSITIVE 291 129 82 167 106 aact:AA01161|5end_araC-like_transcriptional_regulator_794121..794351_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 345 116 78 89 50 aact:AA00990|3end_conserved_hypothetical_protein_693265..693415_POSITIVE 321 123 81 94 56 aact:AA01270|3end_hydrolase_(possible_2-haloalkanoic_acid_dehalogenase_I)_863897..864047_POSITIVE 315 120 71 122 80 aact:AA01434|3end_conserved_hypothetical_protein_(possible_o-antigen_ligase)_963021..963171_POSITIVE 304 110 67 59 22 aact:AA00612|3end_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosami_ne_418705..418855_POSITIVE 303 130 99 99 66 aact:AA02715|3end_paraquat-inducible_protein_pqiB_homolog_1899787..1899937_POSITIVE 293 146 101 94 45 aact:AA01557|3end_recombinase_A_1045965..1046115_POSITIVE 293 140 96 103 56 aact:AA00482|3end_hydrolase_(haloacid_dehalogenase-like)_(HAD_superfamily)_336484..336634_POSITIVE 288 109 67 40 4 aact:AA02886|3end_OBG-related_GTP-binding_protein_2024634..2024784_POSITIVE 284 110 62 150 95 aact:AA00271|3end_hypothetical_protein_190469..190619_POSITIVE 282 124 97 84 52 aact:AA01855|3end_transcriptional_regulator,_Cro/CI_family_1247385..1247535_POSITIVE 279 115 71 65 29 aact:AA00046|3end_formate_dependent_nitrite_reductase,_cytochrome-c_type_protein_36756..36906_POSITIVE 278 216 171 55 4 aact:AA02177|3end_conserved_hypothetical_protein_1489432..1489582_POSITIVE 277 110 61 70 23 aact:AA01090|3end_leucyl-tRNA_synthetase,_leucyl-tRNA_ligase_756213..756363_POSITIVE 274 140 94 143 90 aact:AA02851|3end_hypothetical_protein_2001068..2001218_POSITIVE 274 140 94 70 17 aact:AA02850|3end_dATP_pyrophosphohydrolase_2000995..2001145_POSITIVE 274 109 61 139 84 aact:AA02742|3end_tRNA_nucleotidyltransferase_1922698..1922848_POSITIVE 274 119 82 140 95 aact:AA01160|3end_ADP_compounds_hydrolase_794190..794340_POSITIVE 271 110 69 49 3 aact:AA02899|3end_oligopeptide_ABC_transporter,_ATP-binding_protein_2031138..2031288_POSITIVE 268 110 70 64 33 aact:AA01852|3end_peptide_chain_release_factor_3_1246551..1246701_POSITIVE 268 126 96 89 55 aact:AA01280|3end_groES_chaperonin_868530..868680_POSITIVE