Origin IGS: tagttagaaggaaagtgcggtggctttagaaaacttaatgacatagcagacaaacaaggctgtttttatcaatttttgctcataaattcaaagaagtgggtgaaccccataccagtattatcaggttttaactcactttttctaacctattgagctta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taagctcaataggttagaaaaagtgagttaaaacctgataatactggtatggggttcacccacttctttgaatttatgagcaaaaattgataaaaacagccttgtttgtctgctatgtcattaagttttctaaagccaccgcactttccttctaacta Mask Tandem Repeat Region ================================================ tagttagaaggaaagtgcggtggctttagaaaacttaatgacatagcagacaaacaaggctgtttttatcaatttttgctcataaattcaaagaagtgggtgaaccccataccagtattatcaggttttaactcactttttctaacctattgagctta Find is-nt database================================================ Query_seq: AA01983:AA01984|AA01983:AA01984:maltose regulon positive regulatory protein:tellurite resistance protein; hemagglutinin homolog:->->:1344799..1344956 158 tagttagaaggaaagtgcggtggctttagaaaacttaatgacatagcagacaaacaaggctgtttttatcaatttttgctcataaattcaaagaagtgggtgaaccccataccagtattatcaggttttaactcactttttctaacctattgagctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01983:AA01984|AA01983:AA01984:maltose regulon positive regulatory protein:tellurite resistance protein; hemagglutinin homolog:->->:1344799..1344956 158 tagttagaaggaaagtgcggtggctttagaaaacttaatgacatagcagacaaacaaggctgtttttatcaatttttgctcataaattcaaagaagtgggtgaaccccataccagtattatcaggttttaactcactttttctaacctattgagctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01983:AA01984|AA01983:AA01984:maltose regulon positive regulatory protein:tellurite resistance protein; hemagglutinin homolog:->->:1344799..1344956 158 tagttagaaggaaagtgcggtggctttagaaaacttaatgacatagcagacaaacaaggctgtttttatcaatttttgctcataaattcaaagaagtgggtgaaccccataccagtattatcaggttttaactcactttttctaacctattgagctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagttagaaggaaagtgcggtggctttagaaaacttaatgacatagcagacaaacaaggctgtttttatcaatttttgctcataaattcaaagaagtgggtgaaccccataccagtattatcaggttttaactcactttttctaacctattgagctta Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 43 End: 150 .......................................... M A D K Q G C F Y Q F L L I N S K K W V N P I P V L S G F N S L F L T Y ........ tagttagaaggaaagtgcggtggctttagaaaacttaatgacatagcagacaaacaaggctgtttttatcaatttttgctcataaattcaaagaagtgggtgaaccccataccagtattatcaggttttaactcactttttctaacctattgagctta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagttagaaggaaagtgcggtggctttagaaaacttaatgacatagcagacaaacaaggctgtttttatcaatttttgctcataaattcaaagaagtgggtgaaccccataccagtattatcaggttttaactcactttttctaacctattgagctta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01983:AA01984|AA01983:AA01984:maltose regulon positive regulatory protein:tellurite resistance protein; hemagglutinin homolog:->->:1344799..1344956 158 tagttagaaggaaagtgcggtggctttagaaaacttaatgacatagcagacaaacaaggctgtttttatcaatttttgctcataaattcaaagaagtgggtgaaccccataccagtattatcaggttttaactcactttttctaacctattgagctta Intra-Species Hit: Count: 1 Min: 1 Max: 158 Len: 158 Subject: aact_AA01983_AA01984|maltose regulon positive regulatory protein:tellurite resistance protein; hemagglutinin homolog|POSITIVE:POSITIVE|[1344799,1344956]|158 HSP 1 e-value: 9.0E-85 bit: 313.0 Len: 158 Query Start:1 Query End:158 Subject Strand: POSITIVE Subject Start: 1 Subject End: 158 tagttagaaggaaagtgcggtggctttagaaaacttaatgacatagcagacaaacaaggctgtttttatcaatttttgctcataaattcaaagaagtgggtgaaccccataccagtattatcaggttttaactcactttttctaacctattgagctta tagttagaaggaaagtgcggtggctttagaaaacttaatgacatagcagacaaacaaggctgtttttatcaatttttgctcataaattcaaagaagtgggtgaaccccataccagtattatcaggttttaactcactttttctaacctattgagctta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUUAGAAGGAAAGUGCGGUGGCUUUAGAAAACUUAAUGACAUAGCAGACAAACAAGGCUGUUUUUAUCAAUUUUUGCUCAUAAAUUCAAAGAAGUGGGUGAACCCCAUACCAGUAUUAUCAGGUUUUAACUCACUUUUUCUAACCUAUUGAGCUUA ............((((.((((((..((((((.......(((...(((((.(......).)))))....))).....................((((((((((((((..(((.......)))..)))))..))))))))))))))).)))))).)))). (-29.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGCUCAAUAGGUUAGAAAAAGUGAGUUAAAACCUGAUAAUACUGGUAUGGGGUUCACCCACUUCUUUGAAUUUAUGAGCAAAAAUUGAUAAAAACAGCCUUGUUUGUCUGCUAUGUCAUUAAGUUUUCUAAAGCCACCGCACUUUCCUUCUAACUA ...(((((.((((((.(((.(((((.((...(((((.(((.......))).))))).)).))))).))).)))))))))))......(((((....(((.(......).)))...)))))...((((....((((........))))......)))). (-28.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 322 140 92 143 94 aact:AA02366|5end_hypothetical_protein_1623020..1623250_POSITIVE 299 50 8 98 54 aact:AA00906|5end_sigma-E_factor_regulatory_protein_625110..625340_POSITIVE 296 48 8 159 111 aact:AA01967|5end_hypothetical_protein_1331511..1331741_POSITIVE 292 50 8 79 45 aact:AA01455|5end_D-xylose_ABC_transporter,_periplasmic-binding_protein_975031..975261_POSITIVE 268 48 9 216 172 aact:AA02778|5end_hypothetical_protein_1942585..1942815_POSITIVE 266 140 95 83 33 aact:AA00167|5end_possible_amino-acid_transporter_(sodium-alanine_symporters)_118239..118469_POSITIVE 265 60 11 149 71 aact:AA02987|5end_histidine_triad_protein_homolog_2088767..2088997_POSITIVE 259 117 73 128 71 aact:AA02819|5end_DNA_polymerase_III,_psi_subunit_1981796..1982026_POSITIVE 259 48 9 147 103 aact:AA00386|5end_aspartate--ammonia_ligase_(asparagine_synthetase_A)_261150..261380_POSITIVE 257 29 2 123 89 aact:AA00789|5end_DNA_topoisomerase_III_540486..540716_POSITIVE 254 60 14 61 18 aact:AA02895|5end_oligopeptide_transport_system_permease_protein,_C-terminal_2027484..2027714_POSITIVE 254 23 6 123 106 aact:AA02597|5end_ribonuclease_D_1807124..1807354_POSITIVE 254 29 3 214 179 aact:AA02195|5end_oxaloacetate_decarboxylase_alpha_chain_1494501..1494731_POSITIVE 253 150 108 91 40 aact:AA00469|5end_inorganic_pyrophosphatase,_chain_A_326206..326436_POSITIVE 250 49 10 40 11 aact:AA00191|5end_conserved_hypothetical_protein_(possible_acetyltransferase,_GNAT_family)_135019..135249_POSITIVE 249 29 15 129 115 aact:AA01681|5end_molybdate-binding_periplasmic_protein_1135159..1135389_POSITIVE 247 60 14 206 161 aact:AA02718|5end_spermidine/putrescine_ABC_transporter,_ATP-binding_protein_1901294..1901524_POSITIVE 246 48 3 170 128 aact:AA00390|5end_permease_263689..263919_POSITIVE 245 39 9 119 87 aact:AA01678|5end_possible_transcriptional_regulator_1133819..1134049_POSITIVE 242 107 63 125 81 aact:AA00743|5end_conserved_hypothetical_protein_(possible_membrane_protein)_508371..508601_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 296 48 8 82 34 aact:AA01966|3end_2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_pyrophosphokinase_1331514..1331664_POSITIVE 268 48 9 78 34 aact:AA02777|3end_tRNA-guanine_transglycosylase_1942527..1942677_POSITIVE 266 34 1 59 31 aact:AA02748|3end_conserved_hypothetical_protein_1925582..1925732_POSITIVE 259 117 73 72 15 aact:AA02818|3end_ribosomal_RNA_small_subunit_methyltransferase_C_1981820..1981970_POSITIVE 259 48 9 85 41 aact:AA00384|3end_conserved_hypothetical_protein_(possible_nitrogen_fixation_protein)_261168..261318_POSITIVE 254 23 6 29 12 aact:AA02595|3end_cytochrome_c_peroxidase_1807110..1807260_POSITIVE 249 60 15 41 1 aact:AA02893|3end_oligopeptide_transport_system_permease_protein,_N-terminal_2027544..2027694_POSITIVE 249 29 15 126 112 aact:AA01679|3end_aspartate-semialdehyde_dehydrogenase;_PTS_system_enzyme_II_A_component_1135236..1135386_POSITIVE 247 60 14 144 99 aact:AA02717|3end_hypothetical_protein_1901312..1901462_POSITIVE 242 138 96 147 92 aact:AA01220|3end_competence_protein_F;_amidophosphoribosyltransferase_833538..833688_POSITIVE 242 107 63 103 59 aact:AA00742|3end_hypothetical_protein_508429..508579_POSITIVE 241 32 5 116 88 aact:AA01978|3end_hypothetical_protein_1337257..1337407_POSITIVE 240 33 12 82 60 aact:AA01040|3end_possible_lipoprotein_724946..725096_POSITIVE 239 50 5 81 36 aact:AA01288|3end_hypothetical_protein_872288..872438_POSITIVE 239 23 2 138 117 aact:AA00177|3end_conserved_hypothetical_protein_126313..126463_POSITIVE 238 119 76 120 77 aact:AA02811|3end_hypothetical_protein_1980105..1980255_POSITIVE 236 103 62 34 1 aact:AA02543|3end_conserved_hypothetical_protein_1773434..1773584_POSITIVE 235 23 6 94 79 aact:AA00064|3end_ribosomal_small_subunit_pseudouridine_synthase_48946..49096_POSITIVE 233 50 5 85 38 aact:AA01755|3end_cobalt_membrane_transport_protein_1186555..1186705_POSITIVE 232 59 11 108 52 aact:AA01672|3end_conserved_hypothetical_protein_1131518..1131668_POSITIVE