Origin IGS: aaaaggggctcctgattaatcaatttatttcgtggttttattatgtttaatcaaagtaaaacgaaatcctccctgctataatcttgccggtatgacaacaaggaagtaattcgttagactaggtaggagaaatcctccttctttcgttatagttcaattcacgattaaaatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aattttaatcgtgaattgaactataacgaaagaaggaggatttctcctacctagtctaacgaattacttccttgttgtcataccggcaagattatagcagggaggatttcgttttactttgattaaacataataaaaccacgaaataaattgattaatcaggagcccctttt Mask Tandem Repeat Region ================================================ aaaaggggctcctgattaatcaatttatttcgtggttttattatgtttaatcaaagtaaaacgaaatcctccctgctataatcttgccggtatgacaacaaggaagtaattcgttagactaggtaggagaaatcctccttctttcgttatagttcaattcacgattaaaatt Find is-nt database================================================ Query_seq: AA01982:AA01983|AA01982:AA01983:maltodextrin phosphorylase:maltose regulon positive regulatory protein:->->:1341915..1342086 172 aaaaggggctcctgattaatcaatttatttcgtggttttattatgtttaatcaaagtaaaacgaaatcctccctgctataatcttgccggtatgacaacaaggaagtaattcgttagactaggtaggagaaatcctccttctttcgttatagttcaattcacgattaaaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01982:AA01983|AA01982:AA01983:maltodextrin phosphorylase:maltose regulon positive regulatory protein:->->:1341915..1342086 172 aaaaggggctcctgattaatcaatttatttcgtggttttattatgtttaatcaaagtaaaacgaaatcctccctgctataatcttgccggtatgacaacaaggaagtaattcgttagactaggtaggagaaatcctccttctttcgttatagttcaattcacgattaaaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01982:AA01983|AA01982:AA01983:maltodextrin phosphorylase:maltose regulon positive regulatory protein:->->:1341915..1342086 172 aaaaggggctcctgattaatcaatttatttcgtggttttattatgtttaatcaaagtaaaacgaaatcctccctgctataatcttgccggtatgacaacaaggaagtaattcgttagactaggtaggagaaatcctccttctttcgttatagttcaattcacgattaaaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaaaggggctcctgattaatcaatttatttcgtggttttattatgtttaatcaaagtaaaacgaaatcctccctgctataatcttgccggtatgacaacaaggaagtaattcgttagactaggtaggagaaatcctccttctttcgttatagttcaattcacgattaaaatt Predict ORF larger than 30AA ================================================ aaaaggggctcctgattaatcaatttatttcgtggttttattatgtttaatcaaagtaaaacgaaatcctccctgctataatcttgccggtatgacaacaaggaagtaattcgttagactaggtaggagaaatcctccttctttcgttatagttcaattcacgattaaaatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaaggggctcctgattaatcaatttatttcgtggttttattatgtttaatcaaagtaaaacgaaatcctccctgctataatcttgccggtatgacaacaaggaagtaattcgttagactaggtaggagaaatcctccttctttcgttatagttcaattcacgattaaaatt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01982:AA01983|AA01982:AA01983:maltodextrin phosphorylase:maltose regulon positive regulatory protein:->->:1341915..1342086 172 aaaaggggctcctgattaatcaatttatttcgtggttttattatgtttaatcaaagtaaaacgaaatcctccctgctataatcttgccggtatgacaacaaggaagtaattcgttagactaggtaggagaaatcctccttctttcgttatagttcaattcacgattaaaatt Intra-Species Hit: Count: 1 Min: 1 Max: 172 Len: 172 Subject: aact_AA01982_AA01983|maltodextrin phosphorylase:maltose regulon positive regulatory protein|POSITIVE:POSITIVE|[1341915,1342086]|172 HSP 1 e-value: 5.0E-93 bit: 341.0 Len: 172 Query Start:1 Query End:172 Subject Strand: POSITIVE Subject Start: 1 Subject End: 172 aaaaggggctcctgattaatcaatttatttcgtggttttattatgtttaatcaaagtaaaacgaaatcctccctgctataatcttgccggtatgacaacaaggaagtaattcgttagactaggtaggagaaatcctccttctttcgttatagttcaattcacgattaaaatt aaaaggggctcctgattaatcaatttatttcgtggttttattatgtttaatcaaagtaaaacgaaatcctccctgctataatcttgccggtatgacaacaaggaagtaattcgttagactaggtaggagaaatcctccttctttcgttatagttcaattcacgattaaaatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAGGGGCUCCUGAUUAAUCAAUUUAUUUCGUGGUUUUAUUAUGUUUAAUCAAAGUAAAACGAAAUCCUCCCUGCUAUAAUCUUGCCGGUAUGACAACAAGGAAGUAAUUCGUUAGACUAGGUAGGAGAAAUCCUCCUUCUUUCGUUAUAGUUCAAUUCACGAUUAAAAUU ...(((....)))..((((((........((((.((((((((.((......)).)))))))).........((.((.........)).)).)))).......(((.((...((..(((..(((.((((....))))))))))..))...)).))).......)))))).... (-33.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUUUUAAUCGUGAAUUGAACUAUAACGAAAGAAGGAGGAUUUCUCCUACCUAGUCUAACGAAUUACUUCCUUGUUGUCAUACCGGCAAGAUUAUAGCAGGGAGGAUUUCGUUUUACUUUGAUUAAACAUAAUAAAACCACGAAAUAAAUUGAUUAAUCAGGAGCCCCUUUU ........((((.............)))).((((((.((....((((.....(((..((((((...((((((((((((.((.........)).))))))))))))..))))))..))).(((((((.((.....................)).))))))))))))))))))) (-37.92) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 313 98 54 105 57 aact:AA01337|5end_cell_division_protein_N_904235..904465_POSITIVE 304 163 126 129 92 aact:AA01703|5end_L-ribulose-phosphate_4-epimerase_1155389..1155619_POSITIVE 286 150 102 226 191 aact:AA02415|5end_hypothetical_protein_1658443..1658673_POSITIVE 286 108 63 129 77 aact:AA00110|5end_ATPase_involved_in_DNA_replication_79085..79315_POSITIVE 277 116 71 227 172 aact:AA02025|5end_hypothetical_protein_1378667..1378897_POSITIVE 273 130 86 109 63 aact:AA02158|5end_TonB-dependent_outer_membrane_receptor_1476155..1476385_POSITIVE 272 99 55 57 7 aact:AA01296|5end_shikimate_kinase_(shikimic_acid_kinase_I)_878384..878614_POSITIVE 272 105 61 127 82 aact:AA00189|5end_dihydroxynaphthoic_acid_synthase_(naphthoate_synthas)_133119..133349_POSITIVE 264 104 61 125 79 aact:AA00598|5end_hypothetical_protein_408080..408310_POSITIVE 262 130 81 72 21 aact:AA02543|5end_conserved_hypothetical_protein_1772701..1772931_POSITIVE 260 118 73 103 56 aact:AA00465|5end_hypothetical_protein_324206..324436_POSITIVE 257 96 62 85 58 aact:AA00705|5end_Inositol-1-monophosphatase_(IMPase);_extragenic_suppressor_483827..484057_POSITIVE 255 130 86 73 26 aact:AA01040|5end_possible_lipoprotein_724276..724506_POSITIVE 252 104 61 134 93 aact:AA02142|5end_outer_membrane_protein_OprM_precursor_1466037..1466267_POSITIVE 251 116 73 225 181 aact:AA02231|5end_probable_dethiobiotin_synthetase_1_1517650..1517880_POSITIVE 251 96 62 43 1 aact:AA01424|5end_threonine_deaminase_(dehydratase)_957740..957970_POSITIVE 251 92 62 193 162 aact:AA01201|5end_phosphatase;_hydrolase_822513..822743_POSITIVE 249 146 112 155 124 aact:AA00822|5end_conserved_hypothetical_protein_(possible_arsenate_reductase)_564596..564826_POSITIVE 245 154 111 98 54 aact:AA01515|5end_SlyX_family_protein_1020682..1020912_POSITIVE 245 163 122 215 159 aact:AA01450|5end_hypothetical_protein_971860..972090_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 313 98 54 105 57 aact:AA01336|3end_hypothetical_protein_904315..904465_POSITIVE 304 163 126 132 95 aact:AA01700|3end_hexulose-6-phosphate_isomerase_1155472..1155622_POSITIVE 286 108 63 117 65 aact:AA00109|3end_conserved_hypothetical_protein_79153..79303_POSITIVE 272 105 61 84 39 aact:AA00188|3end_conserved_hypothetical_protein_133156..133306_POSITIVE 264 104 61 122 76 aact:AA00596|3end_uridylate_kinase_408157..408307_POSITIVE 262 153 113 35 3 aact:AA00429|3end_conserved_hypothetical_protein_291891..292041_POSITIVE 253 119 71 149 120 aact:AA00377|3end_acetyl-CoA_acetyltransferase_(Beta-ketothiolase,3-ketoacyl-CoA_thiolase)_254300..254450_POSITIVE 252 104 61 111 70 aact:AA02141|3end_ABC_transporter,_permease_protein_1466094..1466244_POSITIVE 251 116 73 112 68 aact:AA02230|3end_sugar_kinase_1517617..1517767_POSITIVE 251 92 62 86 55 aact:AA01199|3end_conserved_hypothetical_protein_822486..822636_POSITIVE 246 116 83 151 112 aact:AA02595|3end_cytochrome_c_peroxidase_1807110..1807260_POSITIVE 245 156 119 70 33 aact:AA01953|3end_hypothetical_protein_1320349..1320499_POSITIVE 244 119 71 132 71 aact:AA00313|3end_hypothetical_protein_213057..213207_POSITIVE 239 92 54 148 104 aact:AA02604|3end_thioredoxin_reductase_1816582..1816732_POSITIVE 232 118 73 101 48 aact:AA01502|3end_serine_acetyltransferase_1010888..1011038_POSITIVE 231 161 122 143 107 aact:AA00590|3end_outer_membrane_substrate_binding_protein;_TonB-dependent_outer_membrane_receptor_403097..403247_POSITIVE 230 106 70 142 107 aact:AA00497|3end_hexose_phosphate_transport_system_regulatory_protein;_regulator_of_uhpT_expression_346479..346629_POSITIVE 229 106 62 145 102 aact:AA02177|3end_conserved_hypothetical_protein_1489432..1489582_POSITIVE 228 90 47 128 84 aact:AA00991|3end_conserved_hypothetical_protein_693741..693891_POSITIVE 227 92 67 111 82 aact:AA02687|3end_hydrogenase-4_component_D_1874043..1874193_POSITIVE