Origin IGS: agcgattccttaaaggattgtctgatttcggttgtgtgttggttagttgatggcacgcgttaggaaacgtgcgccatcagagttttttatcgttcgctaaaaagtgcggtca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaccgcactttttagcgaacgataaaaaactctgatggcgcacgtttcctaacgcgtgccatcaactaaccaacacacaaccgaaatcagacaatcctttaaggaatcgct Mask Tandem Repeat Region ================================================ agcgattccttaaaggattgtctgatttcggttgtgtgttggttagttgatggcacgcgttaggaaacgtgcgccatcagagttttttatcgttcgctaaaaagtgcggtca Find is-nt database================================================ Query_seq: AA01981:AA01982|AA01981:AA01982:4-alpha-glucanotransferase:maltodextrin phosphorylase:->->:1339415..1339526 112 agcgattccttaaaggattgtctgatttcggttgtgtgttggttagttgatggcacgcgttaggaaacgtgcgccatcagagttttttatcgttcgctaaaaagtgcggtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01981:AA01982|AA01981:AA01982:4-alpha-glucanotransferase:maltodextrin phosphorylase:->->:1339415..1339526 112 agcgattccttaaaggattgtctgatttcggttgtgtgttggttagttgatggcacgcgttaggaaacgtgcgccatcagagttttttatcgttcgctaaaaagtgcggtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01981:AA01982|AA01981:AA01982:4-alpha-glucanotransferase:maltodextrin phosphorylase:->->:1339415..1339526 112 agcgattccttaaaggattgtctgatttcggttgtgtgttggttagttgatggcacgcgttaggaaacgtgcgccatcagagttttttatcgttcgctaaaaagtgcggtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agcgattccttaaaggattgtctgatttcggttgtgtgttggttagttgatggcacgcgttaggaaacgtgcgccatcagagttttttatcgttcgctaaaaagtgcggtca Predict ORF larger than 30AA ================================================ agcgattccttaaaggattgtctgatttcggttgtgtgttggttagttgatggcacgcgttaggaaacgtgcgccatcagagttttttatcgttcgctaaaaagtgcggtca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agcgattccttaaaggattgtctgatttcggttgtgtgttggttagttgatggcacgcgttaggaaacgtgcgccatcagagttttttatcgttcgctaaaaagtgcggtca Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 87 HP_score: -5.5 Tail_Score: -5.37345 Start: 45 End: 82 Full_Region: GGTTGTGTGTTGGTT AGTTGATGGCACGCGTT AGGA AACGTGCGCCATCAGAG TTTTTTATCGTTCGC ............................................AGTTGATGGCACGCGTTAGGAAACGTGCGCCATCAGAG.............................. TransTerm Strand: + Conf: 77 HP_score: -9.0 Tail_Score: -3.81322 Start: 46 End: 81 Full_Region: GTTGTGTGTTGGTTA GTTGATGGCACGCGTT AGGA AACGTGCGCCATCAGA GTTTTTTATCGTTCG .............................................GTTGATGGCACGCGTTAGGAAACGTGCGCCATCAGA............................... TransTerm Strand: + Conf: 82 HP_score: -12.5 Tail_Score: -2.87709 Start: 47 End: 80 Full_Region: TTGTGTGTTGGTTAG TTGATGGCACGCGTT AGGA AACGTGCGCCATCAG AGTTTTTTATCGTTC ..............................................TTGATGGCACGCGTTAGGAAACGTGCGCCATCAG................................ Find igs database================================================ Query_seq: AA01981:AA01982|AA01981:AA01982:4-alpha-glucanotransferase:maltodextrin phosphorylase:->->:1339415..1339526 112 agcgattccttaaaggattgtctgatttcggttgtgtgttggttagttgatggcacgcgttaggaaacgtgcgccatcagagttttttatcgttcgctaaaaagtgcggtca Intra-Species Hit: Count: 1 Min: 1 Max: 112 Len: 112 Subject: aact_AA01981_AA01982|4-alpha-glucanotransferase:maltodextrin phosphorylase|POSITIVE:POSITIVE|[1339415,1339526]|112 HSP 1 e-value: 2.0E-57 bit: 222.0 Len: 112 Query Start:1 Query End:112 Subject Strand: POSITIVE Subject Start: 1 Subject End: 112 agcgattccttaaaggattgtctgatttcggttgtgtgttggttagttgatggcacgcgttaggaaacgtgcgccatcagagttttttatcgttcgctaaaaagtgcggtca agcgattccttaaaggattgtctgatttcggttgtgtgttggttagttgatggcacgcgttaggaaacgtgcgccatcagagttttttatcgttcgctaaaaagtgcggtca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGCGAUUCCUUAAAGGAUUGUCUGAUUUCGGUUGUGUGUUGGUUAGUUGAUGGCACGCGUUAGGAAACGUGCGCCAUCAGAGUUUUUUAUCGUUCGCUAAAAAGUGCGGUCA .(((((((......)))))))..(((..((.((..(((.((((...((((((((.((((((....)))))).))))))))........))))..)))....)).))..))). (-32.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGACCGCACUUUUUAGCGAACGAUAAAAAACUCUGAUGGCGCACGUUUCCUAACGCGUGCCAUCAACUAACCAACACACAACCGAAAUCAGACAAUCCUUUAAGGAAUCGCU ..............(((((..............(((((((((.((((....)))).)))))))))..............................(((.....))).))))) (-27.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 578 78 33 181 135 aact:AA02212|5end_ribonucleoside-diphosphate_reductase_beta_chain_1506012..1506242_POSITIVE 432 88 45 222 176 aact:AA02446|5end_conserved_hypothetical_protein_1686841..1687071_POSITIVE 432 100 51 154 106 aact:AA01173|5end_VapD-homolog_803850..804080_POSITIVE 389 98 53 101 53 aact:AA00637|5end_phosphohistidine_phosphatase_438169..438399_POSITIVE 384 110 64 79 42 aact:AA02199|5end_hypothetical_protein_1497627..1497857_POSITIVE 366 110 63 59 9 aact:AA01998|5end_16S_rRNA_pseudouridine_synthase_1356842..1357072_POSITIVE 366 110 69 112 59 aact:AA01918|5end_anaerobic_C4-dicarboxylate_transporter_1290931..1291161_POSITIVE 366 73 36 148 111 aact:AA00531|5end_DNA_polymerase_I_362621..362851_POSITIVE 359 60 16 219 176 aact:AA00099|5end_conserved_hypothetical_protein_72621..72851_POSITIVE 356 99 52 75 36 aact:AA00578|5end_DNA_topoisomerase_394576..394806_POSITIVE 355 80 36 192 129 aact:AA01811|5end_electron_transport_complex_protein_1220336..1220566_POSITIVE 353 58 31 140 110 aact:AA02911|5end_hypothetical_protein_2039006..2039236_POSITIVE 349 79 31 184 129 aact:AA01429|5end_conserved_hypothetical_protein_959581..959811_POSITIVE 345 100 53 94 44 aact:AA01662|5end_molybdopterin_biosynthesis_protein_1126131..1126361_POSITIVE 344 58 13 104 48 aact:AA01416|5end_2C-methyl-D-erythritol_2,4-cyclodiphosphate_952780..953010_POSITIVE 342 69 22 222 175 aact:AA02025|5end_hypothetical_protein_1378667..1378897_POSITIVE 338 69 23 61 16 aact:AA02999|5end_conserved_hypothetical_protein_2094275..2094505_POSITIVE 337 79 33 172 129 aact:AA00482|5end_hydrolase_(haloacid_dehalogenase-like)_(HAD_superfamily)_335232..335462_POSITIVE 333 78 31 189 143 aact:AA01807|5end_electron_transport_complex_protein_1216696..1216926_POSITIVE 331 110 63 150 100 aact:AA02006|5end_PEP--fructosephosphotransferase_system_repressor,_DeoR_family_1363930..1364160_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 535 78 35 45 1 aact:AA02210|3end_ribonucleoside-diphosphate_reductase_alpha_chain_1505956..1506106_POSITIVE 466 80 33 108 45 aact:AA01660|3end_hypothetical_protein_1125282..1125432_POSITIVE 432 100 51 71 23 aact:AA01172|3end_conserved_hypothetical_protein_803847..803997_POSITIVE 384 110 64 64 27 aact:AA02196|3end_oxaloacetate_decarboxylase_beta_chain_1497692..1497842_POSITIVE 368 98 56 46 1 aact:AA00636|3end_MrsA_protein_(phosphoglucomutase/phosphomannomutase)_438194..438344_POSITIVE 366 110 69 97 44 aact:AA01917|3end_hypothetical_protein_1290996..1291146_POSITIVE 366 73 36 115 78 aact:AA00530|3end_hypothetical_protein_362668..362818_POSITIVE 356 99 52 67 28 aact:AA00576|3end_conserved_hypothetical_protein_(possible_translation_factor)_394648..394798_POSITIVE 349 79 31 148 93 aact:AA01428|3end_acetolactate_synthase_959625..959775_POSITIVE 345 100 53 75 25 aact:AA01661|3end_molybdopterin_biosynthesis_protein_1126192..1126342_POSITIVE 344 58 13 104 48 aact:AA01413|3end_conserved_hypothetical_protein_952860..953010_POSITIVE 338 69 23 56 11 aact:AA02998|3end_conserved_hypothetical_protein_2094350..2094500_POSITIVE 337 79 33 59 16 aact:AA00480|3end_mannose-specific_phosphotransferase_system_IID_335199..335349_POSITIVE 336 58 16 143 99 aact:AA01810|3end_electron_transport_complex_protein_1220406..1220556_POSITIVE 329 99 56 146 98 aact:AA00523|3end_conserved_hypothetical_protein_358929..359079_POSITIVE 328 80 31 64 12 aact:AA00369|3end_50S_ribosomal_protein_L3_248680..248830_POSITIVE 325 93 54 55 8 aact:AA01772|3end_shikimate_5-dehydrogenase_protein_1194942..1195092_POSITIVE 320 50 2 149 112 aact:AA02910|3end_ribonuclease_E_2039105..2039255_POSITIVE 320 73 31 151 109 aact:AA02774|3end_collagenase_prtC_homolog,_possible_protease_1938335..1938485_POSITIVE 314 77 39 138 92 aact:AA01775|3end_hypothetical_protein_1196182..1196332_POSITIVE