Origin IGS: aatccgttcaaataacgaacgggtaatagaaggttaaaccgccataggatacacctttcaccatacaaaataaattctcaacagcaaaaaacacgacattaatcgaaaaatttggtgattttggtgaacgttaaatttcgtgattcacgttctgagctaagataccggggggattataaaaaatagcggctttatgttatggtgagcgtcaagttaaaaagaaggataaggtagtac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gtactaccttatccttctttttaacttgacgctcaccataacataaagccgctattttttataatccccccggtatcttagctcagaacgtgaatcacgaaatttaacgttcaccaaaatcaccaaatttttcgattaatgtcgtgttttttgctgttgagaatttattttgtatggtgaaaggtgtatcctatggcggtttaaccttctattacccgttcgttatttgaacggatt Mask Tandem Repeat Region ================================================ aatccgttcaaataacgaacgggtaatagaaggttaaaccgccataggatacacctttcaccatacaaaataaattctcaacagcaaaaaacacgacattaatcgaaaaatttggtgattttggtgaacgttaaatttcgtgattcacgttctgagctaagataccggggggattataaaaaatagcggctttatgttatggtgagcgtcaagttaaaaagaaggataaggtagtac Find is-nt database================================================ Query_seq: AA01973:AA01974|AA01973:AA01974:acetate kinase:conserved hypothetical protein:->->:1335794..1336030 237 aatccgttcaaataacgaacgggtaatagaaggttaaaccgccataggatacacctttcaccatacaaaataaattctcaacagcaaaaaacacgacattaatcgaaaaatttggtgattttggtgaacgttaaatttcgtgattcacgttctgagctaagataccggggggattataaaaaatagcggctttatgttatggtgagcgtcaagttaaaaagaaggataaggtagtac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01973:AA01974|AA01973:AA01974:acetate kinase:conserved hypothetical protein:->->:1335794..1336030 237 aatccgttcaaataacgaacgggtaatagaaggttaaaccgccataggatacacctttcaccatacaaaataaattctcaacagcaaaaaacacgacattaatcgaaaaatttggtgattttggtgaacgttaaatttcgtgattcacgttctgagctaagataccggggggattataaaaaatagcggctttatgttatggtgagcgtcaagttaaaaagaaggataaggtagtac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01973:AA01974|AA01973:AA01974:acetate kinase:conserved hypothetical protein:->->:1335794..1336030 237 aatccgttcaaataacgaacgggtaatagaaggttaaaccgccataggatacacctttcaccatacaaaataaattctcaacagcaaaaaacacgacattaatcgaaaaatttggtgattttggtgaacgttaaatttcgtgattcacgttctgagctaagataccggggggattataaaaaatagcggctttatgttatggtgagcgtcaagttaaaaagaaggataaggtagtac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatccgttcaaataacgaacgggtaatagaaggttaaaccgccataggatacacctttcaccatacaaaataaattctcaacagcaaaaaacacgacattaatcgaaaaatttggtgattttggtgaacgttaaatttcgtgattcacgttctgagctaagataccggggggattataaaaaatagcggctttatgttatggtgagcgtcaagttaaaaagaaggataaggtagtac Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: - Start: 11 End: 103 .......... I V F P Y Y F T L G G Y S V G K V M C F L N E V A F F V V N M ...................................................................................................................................... aatccgttcaaataacgaacgggtaatagaaggttaaaccgccataggatacacctttcaccatacaaaataaattctcaacagcaaaaaacacgacattaatcgaaaaatttggtgattttggtgaacgttaaatttcgtgattcacgttctgagctaagataccggggggattataaaaaatagcggctttatgttatggtgagcgtcaagttaaaaagaaggataaggtagtac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatccgttcaaataacgaacgggtaatagaaggttaaaccgccataggatacacctttcaccatacaaaataaattctcaacagcaaaaaacacgacattaatcgaaaaatttggtgattttggtgaacgttaaatttcgtgattcacgttctgagctaagataccggggggattataaaaaatagcggctttatgttatggtgagcgtcaagttaaaaagaaggataaggtagtac Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01973:AA01974|AA01973:AA01974:acetate kinase:conserved hypothetical protein:->->:1335794..1336030 237 aatccgttcaaataacgaacgggtaatagaaggttaaaccgccataggatacacctttcaccatacaaaataaattctcaacagcaaaaaacacgacattaatcgaaaaatttggtgattttggtgaacgttaaatttcgtgattcacgttctgagctaagataccggggggattataaaaaatagcggctttatgttatggtgagcgtcaagttaaaaagaaggataaggtagtac Intra-Species Hit: Count: 1 Min: 1 Max: 237 Len: 237 Subject: aact_AA01973_AA01974|acetate kinase:conserved hypothetical protein|POSITIVE:POSITIVE|[1335794,1336030]|237 HSP 1 e-value: 1.0E-131 bit: 470.0 Len: 237 Query Start:1 Query End:237 Subject Strand: POSITIVE Subject Start: 1 Subject End: 237 aatccgttcaaataacgaacgggtaatagaaggttaaaccgccataggatacacctttcaccatacaaaataaattctcaacagcaaaaaacacgacattaatcgaaaaatttggtgattttggtgaacgttaaatttcgtgattcacgttctgagctaagataccggggggattataaaaaatagcggctttatgttatggtgagcgtcaagttaaaaagaaggataaggtagtac aatccgttcaaataacgaacgggtaatagaaggttaaaccgccataggatacacctttcaccatacaaaataaattctcaacagcaaaaaacacgacattaatcgaaaaatttggtgattttggtgaacgttaaatttcgtgattcacgttctgagctaagataccggggggattataaaaaatagcggctttatgttatggtgagcgtcaagttaaaaagaaggataaggtagtac Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUCCGUUCAAAUAACGAACGGGUAAUAGAAGGUUAAACCGCCAUAGGAUACACCUUUCACCAUACAAAAUAAAUUCUCAACAGCAAAAAACACGACAUUAAUCGAAAAAUUUGGUGAUUUUGGUGAACGUUAAAUUUCGUGAUUCACGUUCUGAGCUAAGAUACCGGGGGGAUUAUAAAAAAUAGCGGCUUUAUGUUAUGGUGAGCGUCAAGUUAAAAAGAAGGAUAAGGUAGUAC .((((((((.......)))))))).......(((......)))......((((((((...((..........(((((((..............(((......))).......(((((.(((((((((((((..((((....)))).))))))...)))))))))))).)))))))............(((((.(((((......))))).)))))........))..))))).))). (-49.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUACUACCUUAUCCUUCUUUUUAACUUGACGCUCACCAUAACAUAAAGCCGCUAUUUUUUAUAAUCCCCCCGGUAUCUUAGCUCAGAACGUGAAUCACGAAAUUUAACGUUCACCAAAAUCACCAAAUUUUUCGAUUAAUGUCGUGUUUUUUGCUGUUGAGAAUUUAUUUUGUAUGGUGAAAGGUGUAUCCUAUGGCGGUUUAACCUUCUAUUACCCGUUCGUUAUUUGAACGGAUU ((((.(((((.....((..........))...((((((((.((....((((...................)))).(((((((...((((((((((......)))).))))))...........((((.....((((....)))).....))))..)))))))........)))))))))))))))))))...((((.((.....))..))))...(((((((.....)))))))... (-46.71) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 332 46 1 227 184 aact:AA00148|5end_ATP-dependent_RNA_helicase_105955..106185_POSITIVE 284 169 122 220 168 aact:AA01360|5end_XAA-pro_aminopeptidase_(X-pro_aminopeptidase)_921047..921277_POSITIVE 283 210 166 64 18 aact:AA01349|5end_NAD(P)H_nitroreductase/H-flavin_oxidoreductase_913619..913849_POSITIVE 277 56 16 226 183 aact:AA02006|5end_PEP--fructosephosphotransferase_system_repressor,_DeoR_family_1363930..1364160_POSITIVE 271 82 43 156 120 aact:AA01053|5end_conserved_hypothetical_protein_733119..733349_POSITIVE 271 170 123 195 153 aact:AA00260|5end_conserved_hypothetical_protein_183315..183545_POSITIVE 268 225 186 182 145 aact:AA00177|5end_conserved_hypothetical_protein_125964..126194_POSITIVE 267 60 15 72 15 aact:AA02937|5end_conserved_hypothetical_protein_2056379..2056609_POSITIVE 267 211 186 114 85 aact:AA02692|5end_hydrogenase_4_subunit;_hydrogenase_3_subunit_1876324..1876554_POSITIVE 267 50 21 31 1 aact:AA00506|5end_ATP-dependent_RNA_helicase_protein_351681..351911_POSITIVE 266 170 121 153 108 aact:AA02747|5end_ribose-phosphate_pyrophosphokinase;_phosphoribosylpyrophosphate_synthase_1924324..1924554_POSITIVE 264 172 139 75 40 aact:AA02954|5end_penicillin_tolerance_protein;_hydroxymethylbutenyl_pyrophosphate_reductase_2065610..2065840_POSITIVE 264 170 121 106 61 aact:AA01066|5end_conserved_hypothetical_protein_738380..738610_POSITIVE 263 43 3 201 164 aact:AA01313|5end_deoxyuridinetriphosphatase;_deoxyuridine_5'-triphosphate_nucleotidohydrolase_889567..889797_POSITIVE 260 48 5 168 134 aact:AA00298|5end_hypothetical_protein_203624..203854_POSITIVE 260 49 5 95 60 aact:AA00119|5end_ATP-dependent_Clp_protease_proteolytic_subunit_(endopeptidase_Clp)_88387..88617_POSITIVE 259 210 166 161 126 aact:AA00729|5end_possible_hemolysin_(TlyC-like)_500204..500434_POSITIVE 259 209 166 150 108 aact:AA00207|5end_D-ribose_ABC_transporter,_ATP-binding_protein_/_galactoside_ABC_transporter,_ATP-binding_protein_142592..142822_POSITIVE 258 208 166 139 85 aact:AA02736|5end_conserved_hypothetical_protein_1917350..1917580_POSITIVE 258 208 166 160 116 aact:AA02157|5end_TonB-dependent_outer_membrane_receptor_1475251..1475481_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 363 50 1 144 93 aact:AA00146|3end_UDP-glucose/GDP-mannose_dehydrogenase_105944..106094_POSITIVE 283 210 166 64 18 aact:AA01348|3end_hypothetical_protein_913699..913849_POSITIVE 271 82 43 129 93 aact:AA01051|3end_iron_(III)_ABC_transporter,_ATP-binding_protein_733172..733322_POSITIVE 271 170 123 132 90 aact:AA00259|3end_conserved_hypothetical_protein_183332..183482_POSITIVE 264 172 139 72 37 aact:AA02952|3end_lipoprotein_signal_peptidase_2065687..2065837_POSITIVE 261 46 2 151 112 aact:AA01457|3end_D-xylose_ABC_transporter,_membrane_spanning_protein_978808..978958_POSITIVE 260 208 166 68 27 aact:AA03009|3end_hypothetical_protein_2101236..2101386_POSITIVE 260 49 5 46 11 aact:AA00117|3end_hypothetical_protein_88418..88568_POSITIVE 259 209 166 136 94 aact:AA00205|3end_ribose_ABC_transporter_protein_/_high_affinity_D-ribose_transport_protein_142658..142808_POSITIVE 258 208 166 133 79 aact:AA02735|3end_DNA_repair_protein_1917424..1917574_POSITIVE 258 170 121 117 61 aact:AA00758|3end_hypothetical_protein_520696..520846_POSITIVE 255 210 162 69 13 aact:AA01184|3end_hypothetical_protein_811133..811283_POSITIVE 251 215 188 70 44 aact:AA02001|3end_conserved_hypothetical_protein_1360239..1360389_POSITIVE 251 208 166 99 62 aact:AA00254|3end_penicillin-binding_protein_5_178774..178924_POSITIVE 249 210 162 77 27 aact:AA02101|3end_penicillin-binding_protein_7_1431188..1431338_POSITIVE 249 190 141 148 97 aact:AA01682|3end_molybdate_ABC_transporter_protein_1136442..1136592_POSITIVE 249 147 114 45 9 aact:AA00269|3end_3-oxoacyl-(acyl-carrier-protein)_reductase/short-chain_alcohol_dehydrogenase_189413..189563_POSITIVE 243 60 16 64 10 aact:AA02032|3end_hypothetical_protein_1385294..1385444_POSITIVE 243 209 165 55 2 aact:AA01311|3end_transcriptional_regulator_889644..889794_POSITIVE 243 49 20 41 11 aact:AA00600|3end_ribosome_recycling_factor_(ribosome_releasing_factor)_408939..409089_POSITIVE