Origin IGS: aaagatgaaattacggttcctgagcttgtcgaaggatgagtaatttcatcctatttaggcacaaaactcatgcttcgactaattcttcgacaagctcaggaaccgtctcagcaaccgttttttacatttgctacatagtgccggtgttttttgacatgaaaaacgttgtgaaaaaggaccgcacttttttccaaccta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taggttggaaaaaagtgcggtcctttttcacaacgtttttcatgtcaaaaaacaccggcactatgtagcaaatgtaaaaaacggttgctgagacggttcctgagcttgtcgaagaattagtcgaagcatgagttttgtgcctaaataggatgaaattactcatccttcgacaagctcaggaaccgtaatttcatcttt Mask Tandem Repeat Region ================================================ aaagatgaaattacggttcctgagcttgtcgaaggatgagtaatttcatcctatttaggcacaaaactcatgcttcgactaattcttcgacaagctcaggaaccgtctcagcaaccgttttttacatttgctacatagtgccggtgttttttgacatgaaaaacgttgtgaaaaaggaccgcacttttttccaaccta Find is-nt database================================================ Query_seq: AA01953:AA01954|AA01953:AA01954:hypothetical protein:excinuclease ABC, subunit C:->->:1320410..1320607 198 aaagatgaaattacggttcctgagcttgtcgaaggatgagtaatttcatcctatttaggcacaaaactcatgcttcgactaattcttcgacaagctcaggaaccgtctcagcaaccgttttttacatttgctacatagtgccggtgttttttgacatgaaaaacgttgtgaaaaaggaccgcacttttttccaaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01953:AA01954|AA01953:AA01954:hypothetical protein:excinuclease ABC, subunit C:->->:1320410..1320607 198 aaagatgaaattacggttcctgagcttgtcgaaggatgagtaatttcatcctatttaggcacaaaactcatgcttcgactaattcttcgacaagctcaggaaccgtctcagcaaccgttttttacatttgctacatagtgccggtgttttttgacatgaaaaacgttgtgaaaaaggaccgcacttttttccaaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01953:AA01954|AA01953:AA01954:hypothetical protein:excinuclease ABC, subunit C:->->:1320410..1320607 198 aaagatgaaattacggttcctgagcttgtcgaaggatgagtaatttcatcctatttaggcacaaaactcatgcttcgactaattcttcgacaagctcaggaaccgtctcagcaaccgttttttacatttgctacatagtgccggtgttttttgacatgaaaaacgttgtgaaaaaggaccgcacttttttccaaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaagatgaaattacggttcctgagcttgtcgaaggatgagtaatttcatcctatttaggcacaaaactcatgcttcgactaattcttcgacaagctcaggaaccgtctcagcaaccgttttttacatttgctacatagtgccggtgttttttgacatgaaaaacgttgtgaaaaaggaccgcacttttttccaaccta Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 43 End: 135 .......................................... M S S Y L G T K L M L R L I L R Q A Q E P S Q Q P F F T F A T ............................................................... Protein_Len: 54 Strand: + Start: 36 End: 197 ................................... M S N F I L F R H K T H A S T N S S T S S G T V S A T V F Y I C Y I V P V F F D M K N V V K K D R T F F Q P . aaagatgaaattacggttcctgagcttgtcgaaggatgagtaatttcatcctatttaggcacaaaactcatgcttcgactaattcttcgacaagctcaggaaccgtctcagcaaccgttttttacatttgctacatagtgccggtgttttttgacatgaaaaacgttgtgaaaaaggaccgcacttttttccaaccta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaagatgaaattacggttcctgagcttgtcgaaggatgagtaatttcatcctatttaggcacaaaactcatgcttcgactaattcttcgacaagctcaggaaccgtctcagcaaccgttttttacatttgctacatagtgccggtgttttttgacatgaaaaacgttgtgaaaaaggaccgcacttttttccaaccta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01953:AA01954|AA01953:AA01954:hypothetical protein:excinuclease ABC, subunit C:->->:1320410..1320607 198 aaagatgaaattacggttcctgagcttgtcgaaggatgagtaatttcatcctatttaggcacaaaactcatgcttcgactaattcttcgacaagctcaggaaccgtctcagcaaccgttttttacatttgctacatagtgccggtgttttttgacatgaaaaacgttgtgaaaaaggaccgcacttttttccaaccta Intra-Species Hit: Count: 1 Min: 1 Max: 198 Len: 198 Subject: aact_AA01953_AA01954|hypothetical protein:excinuclease ABC, subunit C|POSITIVE:POSITIVE|[1320410,1320607]|198 HSP 1 e-value: 1.0E-108 bit: 392.0 Len: 198 Query Start:1 Query End:198 Subject Strand: POSITIVE Subject Start: 1 Subject End: 198 aaagatgaaattacggttcctgagcttgtcgaaggatgagtaatttcatcctatttaggcacaaaactcatgcttcgactaattcttcgacaagctcaggaaccgtctcagcaaccgttttttacatttgctacatagtgccggtgttttttgacatgaaaaacgttgtgaaaaaggaccgcacttttttccaaccta aaagatgaaattacggttcctgagcttgtcgaaggatgagtaatttcatcctatttaggcacaaaactcatgcttcgactaattcttcgacaagctcaggaaccgtctcagcaaccgttttttacatttgctacatagtgccggtgttttttgacatgaaaaacgttgtgaaaaaggaccgcacttttttccaaccta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAGAUGAAAUUACGGUUCCUGAGCUUGUCGAAGGAUGAGUAAUUUCAUCCUAUUUAGGCACAAAACUCAUGCUUCGACUAAUUCUUCGACAAGCUCAGGAACCGUCUCAGCAACCGUUUUUUACAUUUGCUACAUAGUGCCGGUGUUUUUUGACAUGAAAAACGUUGUGAAAAAGGACCGCACUUUUUUCCAACCUA ......((((..((((((((((((((((((((((((((((....))))))))....(((((.........)))))...........))))))))))))))))))))...(((((..((.....))..)))))....(((((.(((.((((((.(((..........))).)))))).))))))))..))))....... (-71.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGUUGGAAAAAAGUGCGGUCCUUUUUCACAACGUUUUUCAUGUCAAAAAACACCGGCACUAUGUAGCAAAUGUAAAAAACGGUUGCUGAGACGGUUCCUGAGCUUGUCGAAGAAUUAGUCGAAGCAUGAGUUUUGUGCCUAAAUAGGAUGAAAUUACUCAUCCUUCGACAAGCUCAGGAACCGUAAUUUCAUCUUU .(((.(((((...((((((((..(((((.(((..........))).)))))..))).)))))...((((((.(((.....))).))))))..((((((((((((((((((((....((((.((((((....))))))...))))..(((((((......)))))))))))))))))))))))))))..))))).))). (-74.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 430 190 142 167 117 aact:AA02249|5end_thiamine_biosynthesis_protein_1528365..1528595_POSITIVE 383 187 156 122 91 aact:AA01315|5end_DNA/pantothenate_metabolism_flavoprotein_890091..890321_POSITIVE 383 187 156 52 21 aact:AA01314|5end_hypothetical_protein_890021..890251_POSITIVE 369 188 142 169 123 aact:AA02706|5end_2-oxoglutarate_dehydrogenase_E1_component_(decarboxylase_component)_1886843..1887073_POSITIVE 368 188 142 193 149 aact:AA00213|5end_cold_shock_protein_(negative_regulator_of_cspA_transcription)_147071..147301_POSITIVE 365 41 13 153 125 aact:AA02569|5end_hypothetical_protein_1789012..1789242_POSITIVE 365 190 143 207 151 aact:AA00296|5end_conserved_hypothetical_protein_(possible_hemolysin_/_transport_protein)_202384..202614_POSITIVE 365 190 143 90 34 aact:AA00295|5end_hypothetical_protein_202267..202497_POSITIVE 361 188 142 216 164 aact:AA00711|5end_conserved_hypothetical_protein_487893..488123_POSITIVE 358 188 144 181 136 aact:AA02668|5end_gluconate_permease_1856758..1856988_POSITIVE 357 187 142 126 80 aact:AA02980|5end_conserved_hypothetical_protein_2083305..2083535_POSITIVE 353 190 142 179 126 aact:AA01564|5end_transaldolase_B_1049099..1049329_POSITIVE 351 188 142 136 89 aact:AA00422|5end_LapB_membrane_protein_285528..285758_POSITIVE 350 188 141 146 100 aact:AA01213|5end_competence_related_protein_829867..830097_POSITIVE 349 186 141 59 6 aact:AA02442|5end_thioredoxin-family_protein_1685704..1685934_POSITIVE 349 187 141 122 81 aact:AA01537|5end_possible_glucokinase_regulatory_protein_1030616..1030846_POSITIVE 346 189 142 135 82 aact:AA02987|5end_histidine_triad_protein_homolog_2088767..2088997_POSITIVE 346 189 142 180 132 aact:AA00174|5end_ribosomal_protein_L11_methyltransferase_123072..123302_POSITIVE 346 189 142 76 28 aact:AA00173|5end_hypothetical_protein_122968..123198_POSITIVE 345 188 144 201 155 aact:AA02944|5end_30S_ribosomal_protein_S20_2058688..2058918_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 452 119 78 101 50 aact:AA01953|3end_hypothetical_protein_1320349..1320499_POSITIVE 430 190 142 77 27 aact:AA02246|3end_peptidase_E_1528355..1528505_POSITIVE 415 129 85 81 43 aact:AA01149|3end_glycyl-tRNA_synthetase_beta_subunit_788185..788335_POSITIVE 383 187 156 147 116 aact:AA01314|3end_hypothetical_protein_890196..890346_POSITIVE 383 187 156 50 19 aact:AA01313|3end_deoxyuridinetriphosphatase;_deoxyuridine_5'-triphosphate_nucleotidohydrolase_890099..890249_POSITIVE 368 188 142 89 45 aact:AA00212|3end_ribokinase_147047..147197_POSITIVE 365 190 143 81 25 aact:AA00292|3end_dihydrofolate_reductase_202338..202488_POSITIVE 361 186 143 46 1 aact:AA02813|3end_hypothetical_protein_1980298..1980448_POSITIVE 361 188 142 81 29 aact:AA00709|3end_metallocluster_assembly_(NifU_protein)_487838..487988_POSITIVE 357 187 142 49 3 aact:AA02978|3end_sufI_protein_precursor_homolog_2083308..2083458_POSITIVE 352 188 144 76 31 aact:AA01611|3end_anthranilate_synthase_component_II_1089619..1089769_POSITIVE 349 186 141 66 13 aact:AA02440|3end_ABC_transporter,_ATP-binding_protein_1685791..1685941_POSITIVE 345 188 144 85 39 aact:AA02940|3end_conserved_hypothetical_protein_2058652..2058802_POSITIVE 342 190 142 43 3 aact:AA01563|3end_transketolase_isozyme_1_1049043..1049193_POSITIVE 338 187 142 89 40 aact:AA00574|3end_regulatory_protein_392381..392531_POSITIVE 338 189 144 74 35 aact:AA00209|3end_D-ribose_ABC_transporter,_periplasmic-binding_protein_146032..146182_POSITIVE 333 189 156 40 7 aact:AA00545|3end_glutathionylspermidine_synthetase;amidase_374415..374565_POSITIVE 329 188 144 55 8 aact:AA02964|3end_opacity_associated_protein_A_2074128..2074278_POSITIVE 324 150 107 50 11 aact:AA02076|3end_conserved_hypothetical_protein_1411094..1411244_POSITIVE 323 188 142 82 36 aact:AA01423|3end_cell_division_protein_X,_ABC_system_permease_957680..957830_POSITIVE