Origin IGS: taaccctcatcgtttaacttttcgcacttaaagtgcggtcaattttcttggtgtttttcaagtagaaacgttgaaaaaaacgaccgcactttttgatcttttcaatctctcatgccgtctctccataatgtgatccctatcacgattccaagattcttctgcctatttaattgacaactcaattcctgccagcatttaatacataagtaaattcaataagccaagctggaaggatgtaaaaatggcaaaactggaagtaaaaaatatcacgaaaaaattcggtgatttttatgcggcgaataacattacgtttactgcagaagaaggggagttcgtcaccttacttggtccgagcggttgtggcaagacctcactgttgaaattgattgggggtttcatattgccgatgaaggggagattctcatcggcggcaaaaatgtcaatgag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctcattgacatttttgccgccgatgagaatctccccttcatcggcaatatgaaacccccaatcaatttcaacagtgaggtcttgccacaaccgctcggaccaagtaaggtgacgaactccccttcttctgcagtaaacgtaatgttattcgccgcataaaaatcaccgaattttttcgtgatattttttacttccagttttgccatttttacatccttccagcttggcttattgaatttacttatgtattaaatgctggcaggaattgagttgtcaattaaataggcagaagaatcttggaatcgtgatagggatcacattatggagagacggcatgagagattgaaaagatcaaaaagtgcggtcgtttttttcaacgtttctacttgaaaaacaccaagaaaattgaccgcactttaagtgcgaaaagttaaacgatgagggtta Mask Tandem Repeat Region ================================================ taaccctcatcgtttaacttttcgcacttaaagtgcggtcaattttcttggtgtttttcaagtagaaacgttgaaaaaaacgaccgcactttttgatcttttcaatctctcatgccgtctctccataatgtgatccctatcacgattccaagattcttctgcctatttaattgacaactcaattcctgccagcatttaatacataagtaaattcaataagccaagctggaaggatgtaaaaatggcaaaactggaagtaaaaaatatcacgaaaaaattcggtgatttttatgcggcgaataacattacgtttactgcagaagaaggggagttcgtcaccttacttggtccgagcggttgtggcaagacctcactgttgaaattgattgggggtttcatattgccgatgaaggggagattctcatcggcggcaaaaatgtcaatgag Find is-nt database================================================ Query_seq: AA01945:AA01947|AA01945:AA01947:selenophosphate synthase:iron(III)-transport ATP-binding protein:->->:1316083..1316529 447 taaccctcatcgtttaacttttcgcacttaaagtgcggtcaattttcttggtgtttttcaagtagaaacgttgaaaaaaacgaccgcactttttgatcttttcaatctctcatgccgtctctccataatgtgatccctatcacgattccaagattcttctgcctatttaattgacaactcaattcctgccagcatttaatacataagtaaattcaataagccaagctggaaggatgtaaaaatggcaaaactggaagtaaaaaatatcacgaaaaaattcggtgatttttatgcggcgaataacattacgtttactgcagaagaaggggagttcgtcaccttacttggtccgagcggttgtggcaagacctcactgttgaaattgattgggggtttcatattgccgatgaaggggagattctcatcggcggcaaaaatgtcaatgag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01945:AA01947|AA01945:AA01947:selenophosphate synthase:iron(III)-transport ATP-binding protein:->->:1316083..1316529 447 taaccctcatcgtttaacttttcgcacttaaagtgcggtcaattttcttggtgtttttcaagtagaaacgttgaaaaaaacgaccgcactttttgatcttttcaatctctcatgccgtctctccataatgtgatccctatcacgattccaagattcttctgcctatttaattgacaactcaattcctgccagcatttaatacataagtaaattcaataagccaagctggaaggatgtaaaaatggcaaaactggaagtaaaaaatatcacgaaaaaattcggtgatttttatgcggcgaataacattacgtttactgcagaagaaggggagttcgtcaccttacttggtccgagcggttgtggcaagacctcactgttgaaattgattgggggtttcatattgccgatgaaggggagattctcatcggcggcaaaaatgtcaatgag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 18 Min: 242 Max: 436 Len: 195 Subject: gi|89107135|ref|AP_000915.1| predicted ferric transporter subunit [Escherichia coli W3110] HSP 1 e-value: 2.0E-11 bit: 64.7 Len: 168 Query Start:251 Query End:418 Subject Strand: null Subject Start: 7 Subject End: 62 .......................................................................................................................................................................................................................................................... L E V K N I T K K F G D F Y A A N N I T F T A E E G E F V T L L G P S G C G K T S L L K L I G G F I L P M K G R ............................. .......................................................................................................................................................................................................................................................... V E L R N V T K R F G S N T V I D N I N L T I P Q G Q M V T L L G P S G C G K T T I L R L V A G L E K P S E G Q ............................. Subject: gi|89107645|ref|AP_001425.1| fused predicted transporter subunits and ATP-binding components of ABC superfamily [Escherichia coli W3110] HSP 1 e-value: 9.0E-10 bit: 59.3 Len: 168 Query Start:251 Query End:418 Subject Strand: null Subject Start: 330 Subject End: 385 .......................................................................................................................................................................................................................................................... L E V K N I T K K F G D F Y A A N N I T F T A E E G E F V T L L G P S G C G K T S L L K L I G G F I L P M K G R ............................. .......................................................................................................................................................................................................................................................... I E A K E L T K K F G D F A A T D H V N F A V K R G E I F G L L G P N G A G K S T T F K M M C G L L V P T S G Q ............................. Subject: gi|89110756|ref|AP_004536.1| fused maltose transport subunit, ATP-binding component of ABC superfamily and regulatory protein [Escherichia coli W3110] HSP 1 e-value: 4.0E-9 bit: 57.4 Len: 153 Query Start:242 Query End:394 Subject Strand: null Subject Start: 1 Subject End: 51 ................................................................................................................................................................................................................................................. M A K L E V K N I T K K F G D F Y A A N N I T F T A E E G E F V T L L G P S G C G K T S L L K L I G G ..................................................... ................................................................................................................................................................................................................................................. M A S V Q L Q N V T K A W G E V V V S K D I N L D I H E G E F V V F V G P S G C G K S T L L R M I A G ..................................................... Subject: gi|89107706|ref|AP_001486.1| putrescine transporter subunit [Escherichia coli W3110] HSP 1 e-value: 1.0E-8 bit: 55.8 Len: 168 Query Start:251 Query End:418 Subject Strand: null Subject Start: 20 Subject End: 75 .......................................................................................................................................................................................................................................................... L E V K N I T K K F G D F Y A A N N I T F T A E E G E F V T L L G P S G C G K T S L L K L I G G F I L P M K G R ............................. .......................................................................................................................................................................................................................................................... L E I R N L T K S Y D G Q H A V D D V S L T I Y K G E I F A L L G A S G C G K S T L L R M L A G F E Q P S A G Q ............................. Subject: gi|89107237|ref|AP_001017.1| taurine transporter subunit [Escherichia coli W3110] HSP 1 e-value: 3.0E-8 bit: 54.3 Len: 186 Query Start:251 Query End:436 Subject Strand: null Subject Start: 2 Subject End: 63 .......................................................................................................................................................................................................................................................... L E V K N I T K K F G D F Y A A N N I T F T A E E G E F V T L L G P S G C G K T S L L K L I G G F I L P M K G R F S S A A K ........... .......................................................................................................................................................................................................................................................... L Q I S H L Y A D Y G G K P A L E D I N L T L E S G E L L V V L G P S G C G K T T L L N L I A G F V P Y Q H G S I Q L A G K ........... Subject: gi|89107359|ref|AP_001139.1| predicted transporter subunit [Escherichia coli W3110] HSP 1 e-value: 4.0E-8 bit: 53.9 Len: 165 Query Start:251 Query End:415 Subject Strand: null Subject Start: 8 Subject End: 62 .......................................................................................................................................................................................................................................................... L E V K N I T K K F G D F Y A A N N I T F T A E E G E F V T L L G P S G C G K T S L L K L I G G F I L P M K G ................................ .......................................................................................................................................................................................................................................................... L Q L Q N V G Y L A G D A K I L N N I N F S L R A G E F K L I T G P S G C G K S T L L K I V A S L I S P T S G ................................ Subject: gi|89108284|ref|AP_002064.1| predicted spermidine/putrescine transporter subunit [Escherichia coli W3110] HSP 1 e-value: 1.0E-7 bit: 52.4 Len: 147 Query Start:251 Query End:397 Subject Strand: null Subject Start: 5 Subject End: 53 .......................................................................................................................................................................................................................................................... L E V K N I T K K F G D F Y A A N N I T F T A E E G E F V T L L G P S G C G K T S L L K L I G G F .................................................. .......................................................................................................................................................................................................................................................... V E F D N V S R L Y G D V R A V D G V S I A I K D G E F F S M L G P S G S G K T T C L R L I A G F .................................................. Subject: gi|89109236|ref|AP_003016.1| sulfate/thiosulfate transporter subunit [Escherichia coli W3110] HSP 1 e-value: 4.0E-7 bit: 50.4 Len: 144 Query Start:251 Query End:394 Subject Strand: null Subject Start: 3 Subject End: 50 .......................................................................................................................................................................................................................................................... L E V K N I T K K F G D F Y A A N N I T F T A E E G E F V T L L G P S G C G K T S L L K L I G G ..................................................... .......................................................................................................................................................................................................................................................... I E I A N I K K S F G R T Q V L N D I S L D I P S G Q M V A L L G P S G S G K T T L L R I I A G ..................................................... Subject: gi|89107455|ref|AP_001235.1| iron-enterobactin transporter subunit [Escherichia coli W3110] HSP 1 e-value: 2.0E-6 bit: 48.5 Len: 174 Query Start:242 Query End:415 Subject Strand: null Subject Start: 5 Subject End: 62 ................................................................................................................................................................................................................................................. M A K L E V K N I T K K F G D F Y A A N N I T F T A E E G E F V T L L G P S G C G K T S L L K L I G G F I L P M K G ................................ ................................................................................................................................................................................................................................................. V A R L R G E Q L T L G Y G K Y T V A E N L T V E I P D G H F T A I I G P N G C G K S T L L R T L S R L M T P A H G ................................ Subject: gi|89107972|ref|AP_001752.1| polyamine transporter subunit [Escherichia coli W3110] HSP 1 e-value: 3.0E-6 bit: 47.8 Len: 168 Query Start:251 Query End:418 Subject Strand: null Subject Start: 18 Subject End: 73 .......................................................................................................................................................................................................................................................... L E V K N I T K K F G D F Y A A N N I T F T A E E G E F V T L L G P S G C G K T S L L K L I G G F I L P M K G R ............................. .......................................................................................................................................................................................................................................................... V Q L A G I R K C F D G K E V I P Q L D L T I N N G E F L T L L G P S G C G K T T V L R L I A G L E T V D S G R ............................. Subject: gi|89110445|ref|AP_004225.1| fused subunits of D-xylose transporter and ATP-binding components of ABC superfamily [Escherichia coli W3110] HSP 1 e-value: 3.0E-6 bit: 47.8 Len: 144 Query Start:251 Query End:394 Subject Strand: null Subject Start: 5 Subject End: 52 .......................................................................................................................................................................................................................................................... L E V K N I T K K F G D F Y A A N N I T F T A E E G E F V T L L G P S G C G K T S L L K L I G G ..................................................... .......................................................................................................................................................................................................................................................... L E M K N I T K T F G S V K A I D N V C L R L N A G E I V S L C G E N G S G K S T L M K V L C G ..................................................... Subject: gi|89110526|ref|AP_004306.1| fused ribosome-associated ATPases [Escherichia coli W3110] HSP 1 e-value: 4.0E-6 bit: 47.4 Len: 165 Query Start:251 Query End:415 Subject Strand: null Subject Start: 277 Subject End: 331 .......................................................................................................................................................................................................................................................... L E V K N I T K K F G D F Y A A N N I T F T A E E G E F V T L L G P S G C G K T S L L K L I G G F I L P M K G ................................ .......................................................................................................................................................................................................................................................... I E A R D L T M R F G S F V A V D H V N F R I P R G E I F G F L G S N G C G K S T T M K M L T G L L P A S E G ................................ Subject: gi|89108595|ref|AP_002375.1| predicted transporter subunit [Escherichia coli W3110] HSP 1 e-value: 6.0E-6 bit: 46.6 Len: 138 Query Start:257 Query End:394 Subject Strand: null Subject Start: 4 Subject End: 49 ................................................................................................................................................................................................................................................................ V K N I T K K F G D F Y A A N N I T F T A E E G E F V T L L G P S G C G K T S L L K L I G G ..................................................... ................................................................................................................................................................................................................................................................ V K N V S L R L P E S R L L T N V N F T V D K G D I V T L M G P S G C G K S T L F S W M I G ..................................................... Subject: gi|89108752|ref|AP_002532.1| predicted transporter subunit [Escherichia coli W3110] HSP 1 e-value: 6.0E-6 bit: 46.6 Len: 174 Query Start:242 Query End:415 Subject Strand: null Subject Start: 1 Subject End: 58 ................................................................................................................................................................................................................................................. M A K L E V K N I T K K F G D F Y A A N N I T F T A E E G E F V T L L G P S G C G K T S L L K L I G G F I L P M K G ................................ ................................................................................................................................................................................................................................................. M S A I E V K N L V K K F H G Q T V L H G I D L E V K P G E V V A I I G P S G S G K T T L L R S I N L L E Q P E A G ................................ Subject: gi|89110562|ref|AP_004342.1| glycerol-3-phosphate transporter subunit [Escherichia coli W3110] HSP 1 e-value: 6.0E-6 bit: 46.6 Len: 153 Query Start:242 Query End:394 Subject Strand: null Subject Start: 1 Subject End: 52 ................................................................................................................................................................................................................................................. M A K L E V K N I T K K F - G D F Y A A N N I T F T A E E G E F V T L L G P S G C G K T S L L K L I G G ..................................................... ................................................................................................................................................................................................................................................. M A G L K L Q A V T K S W D G K T Q V I K P L T L D V A D G E F I V M V G P S G C G K S T L L R M V A G ..................................................... Subject: gi|89111099|ref|AP_004879.1| fused predicted transporter subunits and ATP-binding components of ABC superfamily [Escherichia coli W3110] HSP 1 e-value: 6.0E-6 bit: 46.6 Len: 165 Query Start:251 Query End:415 Subject Strand: null Subject Start: 324 Subject End: 378 .......................................................................................................................................................................................................................................................... L E V K N I T K K F G D F Y A A N N I T F T A E E G E F V T L L G P S G C G K T S L L K L I G G F I L P M K G ................................ .......................................................................................................................................................................................................................................................... L E V S N L R K S Y G D R L L I D D L S F S I P K G A I V G I I G P N G A G K S T L F R M I S G Q E Q P D S G ................................ Subject: gi|89107660|ref|AP_001440.1| glutamine transporter subunit [Escherichia coli W3110] HSP 1 e-value: 8.0E-6 bit: 46.2 Len: 138 Query Start:251 Query End:388 Subject Strand: null Subject Start: 2 Subject End: 47 .......................................................................................................................................................................................................................................................... L E V K N I T K K F G D F Y A A N N I T F T A E E G E F V T L L G P S G C G K T S L L K L I ........................................................... .......................................................................................................................................................................................................................................................... I E F K N V S K H F G P T Q V L H N I D L N I A Q G E V V V I I G P S G S G K S T L L R C I ........................................................... Subject: gi|89109958|ref|AP_003738.1| predicted toluene transporter subunit [Escherichia coli W3110] HSP 1 e-value: 8.0E-6 bit: 46.2 Len: 165 Query Start:251 Query End:415 Subject Strand: null Subject Start: 9 Subject End: 63 .......................................................................................................................................................................................................................................................... L E V K N I T K K F G D F Y A A N N I T F T A E E G E F V T L L G P S G C G K T S L L K L I G G F I L P M K G ................................ .......................................................................................................................................................................................................................................................... V D M R D V S F T R G N R C I F D N I S L T V P R G K I T A I M G P S G I G K T T L L R L I G G Q I A P D H G ................................ Find nr database================================================ Query_seq: AA01945:AA01947|AA01945:AA01947:selenophosphate synthase:iron(III)-transport ATP-binding protein:->->:1316083..1316529 447 taaccctcatcgtttaacttttcgcacttaaagtgcggtcaattttcttggtgtttttcaagtagaaacgttgaaaaaaacgaccgcactttttgatcttttcaatctctcatgccgtctctccataatgtgatccctatcacgattccaagattcttctgcctatttaattgacaactcaattcctgccagcatttaatacataagtaaattcaataagccaagctggaaggatgtaaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatgtcaatgag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaccctcatcgtttaacttttcgcacttaaagtgcggtcaattttcttggtgtttttcaagtagaaacgttgaaaaaaacgaccgcactttttgatcttttcaatctctcatgccgtctctccataatgtgatccctatcacgattccaagattcttctgcctatttaattgacaactcaattcctgccagcatttaatacataagtaaattcaataagccaagctggaaggatgtaaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatgtcaatgag Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: + Start: 264 End: 377 ....................................................................................................................................................................................................................................................................... M S R K N S V I F M R R I T L R L L Q K K G S S S P Y L V R A V V A R P H C ...................................................................... Protein_Len: 60 Strand: + Start: 203 End: 445 .......................................................................................................................................................................................................... M S K F N K P S W K D V K M A K L E V K N I T K K F G D F Y A A N N I T F T A E E G E F V T L L G P S G C G K T S L L K .. Protein_Len: 30 Strand: + Start: 358 End: 447 ..................................................................................................................................................................................................................................................................................................................................................................... M W Q D L T V E I D W G F H I A D E G E I L I G G K N V N E Protein_Len: 51 Strand: - Start: 292 End: 444 ................................................................................................................................................................................................................................................................................................... A A F L M V N V A S S P S N T V K S P G L P Q P L V E S N F N I P P K M N G I F P L N E D A A F I D M ... Protein_Len: 40 Strand: - Start: 182 End: 301 ..................................................................................................................................................................................... N R G A N L V Y T F E I L W A P L I Y F H C F Q F Y F I D R F F E T I K I R R M .................................................................................................................................................. Protein_Len: 36 Strand: - Start: 105 End: 212 ........................................................................................................ D R M G D R W L T I G I V I G L N K Q R N L Q C S L E Q W C K I C L Y M ........................................................................................................................................................................................................................................... Protein_Len: 30 Strand: - Start: 17 End: 106 ................ S K A S L T R D I K K T N K L Y F R Q F F R G C K K I K E M ..................................................................................................................................................................................................................................................................................................................................................... taaccctcatcgtttaacttttcgcacttaaagtgcggtcaattttcttggtgtttttcaagtagaaacgttgaaaaaaacgaccgcactttttgatcttttcaatctctcatgccgtctctccataatgtgatccctatcacgattccaagattcttctgcctatttaattgacaactcaattcctgccagcatttaatacataagtaaattcaataagccaagctggaaggatgtaaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatgtcaatgag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaccctcatcgtttaacttttcgcacttaaagtgcggtcaattttcttggtgtttttcaagtagaaacgttgaaaaaaacgaccgcactttttgatcttttcaatctctcatgccgtctctccataatgtgatccctatcacgattccaagattcttctgcctatttaattgacaactcaattcctgccagcatttaatacataagtaaattcaataagccaagctggaaggatgtaaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatgtcaatgag Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 82 HP_score: -6.0 Tail_Score: -4.98616 Start: 33 End: 89 Full_Region: CTTTTCGCACTTAAA GTGCGGTCAATTTTCTTGGTGTTTTT CAAGT AGAAACGTTGAAAAAAACGACCGCAC TTTTTGATCTTTTCA ................................GTGCGGTCAATTTTCTTGGTGTTTTTCAAGTAGAAACGTTGAAAAAAACGACCGCAC...................................................................................................................................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: AA01945:AA01947|AA01945:AA01947:selenophosphate synthase:iron(III)-transport ATP-binding protein:->->:1316083..1316529 447 taaccctcatcgtttaacttttcgcacttaaagtgcggtcaattttcttggtgtttttcaagtagaaacgttgaaaaaaacgaccgcactttttgatcttttcaatctctcatgccgtctctccataatgtgatccctatcacgattccaagattcttctgcctatttaattgacaactcaattcctgccagcatttaatacataagtaaattcaataagccaagctggaaggatgtaaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatgtcaatgag Intra-Species Hit: Count: 1 Min: 1 Max: 241 Len: 241 Subject: aact_AA01945_AA01947|selenophosphate synthase:iron(III)-transport ATP-binding protein|POSITIVE:POSITIVE|[1316083,1316529]|447 HSP 1 e-value: 1.0E-121 bit: 436.0 Len: 241 Query Start:1 Query End:241 Subject Strand: POSITIVE Subject Start: 1 Subject End: 241 taaccctcatcgtttaacttttcgcacttaaagtgcggtcaattttcttggtgtttttcaagtagaaacgttgnnnnnnncgaccgcactttttgatcttttcaatctctcatgccgtctctccataatgtgatccctatcacgattccaagattcttctgcctatttaattgacaactcaattcctgccagcatttaatacataagtaaattcaataagccaagctggaaggatgtaaaa.............................................................................................................................................................................................................. taaccctcatcgtttaacttttcgcacttaaagtgcggtcaattttcttggtgtttttcaagtagaaacgttgaaaaaaacgaccgcactttttgatcttttcaatctctcatgccgtctctccataatgtgatccctatcacgattccaagattcttctgcctatttaattgacaactcaattcctgccagcatttaatacataagtaaattcaataagccaagctggaaggatgtaaaa.............................................................................................................................................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACCCUCAUCGUUUAACUUUUCGCACUUAAAGUGCGGUCAAUUUUCUUGGUGUUUUUCAAGUAGAAACGUUGAAAAAAACGACCGCACUUUUUGAUCUUUUCAAUCUCUCAUGCCGUCUCUCCAUAAUGUGAUCCCUAUCACGAUUCCAAGAUUCUUCUGCCUAUUUAAUUGACAACUCAAUUCCUGCCAGCAUUUAAUACAUAAGUAAAUUCAAUAAGCCAAGCUGGAAGGAUGUAAAA .......................(((...(((((((((((..((((.((..(((((((.....)))))))..)).))))..)))))))))))((((.....)))).......))).(((.........((((((....))))))......(((....)))............)))........((((.(((((...............................))))).))))....... (-46.98) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUACAUCCUUCCAGCUUGGCUUAUUGAAUUUACUUAUGUAUUAAAUGCUGGCAGGAAUUGAGUUGUCAAUUAAAUAGGCAGAAGAAUCUUGGAAUCGUGAUAGGGAUCACAUUAUGGAGAGACGGCAUGAGAGAUUGAAAAGAUCAAAAAGUGCGGUCGUUUUUUUCAACGUUUCUACUUGAAAAACACCAAGAAAAUUGACCGCACUUUAAGUGCGAAAAGUUAAACGAUGAGGGUUA ........(((((..(.(((((((.(((......(((((((......((((......((((((....)))))).....))))...(..((((.((((.(((((....)))))))).).))))..).))))))).((((.....))))..(((((((((((((((((((.....((((.....))))......))))))).)))))))))))).....))).))))))).)...)))))... (-60.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 471 92 53 212 174 aact:AA00347|5end_50S_ribosomal_protein_L11_232137..232367_POSITIVE 462 100 51 164 115 aact:AA02673|5end_gluconate_utilization_operon_repressor_1858913..1859143_POSITIVE 443 95 51 179 139 aact:AA00212|5end_ribokinase_146056..146286_POSITIVE 443 95 51 78 38 aact:AA00211|5end_hypothetical_protein_145955..146185_POSITIVE 435 100 51 151 96 aact:AA02066|5end_hypothetical_protein_1405115..1405345_POSITIVE 406 56 19 105 69 aact:AA02662|5end_hypothetical_protein_1853283..1853513_POSITIVE 406 76 31 73 24 aact:AA01877|5end_conserved_hypothetical_protein_1264115..1264345_POSITIVE 403 59 11 132 88 aact:AA01918|5end_anaerobic_C4-dicarboxylate_transporter_1290931..1291161_POSITIVE 400 79 31 184 133 aact:AA01471|5end_type_I_restriction_enzyme_M_(modification_chain)_987321..987551_POSITIVE 398 90 44 227 178 aact:AA02966|5end_conserved_hypothetical_protein_2074223..2074453_POSITIVE 398 90 44 73 24 aact:AA02965|5end_hypothetical_protein_2074069..2074299_POSITIVE 397 40 3 116 80 aact:AA01846|5end_conserved_hypothetical_protein_1241843..1242073_POSITIVE 397 58 16 148 102 aact:AA00705|5end_Inositol-1-monophosphatase_(IMPase);_extragenic_suppressor_483827..484057_POSITIVE 395 58 17 184 133 aact:AA02410|5end_cell_division_protein_1655429..1655659_POSITIVE 392 90 47 152 107 aact:AA02409|5end_DNA_ligase_1653315..1653545_POSITIVE 387 58 12 76 25 aact:AA01176|5end_hypothetical_protein_806309..806539_POSITIVE 382 106 62 149 101 aact:AA01988|5end_dihydrolipoamide_dehydrogenase;_lipoamide_dehydrogenase_1350517..1350747_POSITIVE 381 104 62 207 166 aact:AA01109|5end_oxidoreductase_(possible_short-chain_alcohol_dehydrogenase)_763899..764129_POSITIVE 372 100 64 138 105 aact:AA00454|5end_conserved_hypothetical_protein_318020..318250_POSITIVE 361 92 61 121 90 aact:AA01024|5end_thiamine_biosynthesis_lipoprotein_precursor_716437..716667_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 471 92 53 53 15 aact:AA00345|3end_transcription_antitermination_protein_232058..232208_POSITIVE 462 100 51 88 39 aact:AA02671|3end_gluconokinase_1858917..1859067_POSITIVE 443 95 51 75 35 aact:AA00209|3end_D-ribose_ABC_transporter,_periplasmic-binding_protein_146032..146182_POSITIVE 413 98 56 138 91 aact:AA01259|3end_protoporphyrinogen_oxidase_854943..855093_POSITIVE 406 56 19 89 53 aact:AA02659|3end_conserved_hypothetical_protein_1853347..1853497_POSITIVE 403 59 11 117 73 aact:AA01917|3end_hypothetical_protein_1290996..1291146_POSITIVE 398 90 44 52 3 aact:AA02964|3end_opacity_associated_protein_A_2074128..2074278_POSITIVE 397 58 16 89 43 aact:AA00702|3end_conserved_hypothetical_protein_483848..483998_POSITIVE 396 58 16 88 48 aact:AA00168|3end_possible_sigma_54_modulation_protein_120240..120390_POSITIVE 395 58 17 79 28 aact:AA02409|3end_DNA_ligase_1655404..1655554_POSITIVE 387 58 12 82 31 aact:AA01175|3end_6-phosphogluconate_dehydrogenase_806395..806545_POSITIVE 383 60 17 120 72 aact:AA02135|3end_hypothetical_protein_1459067..1459217_POSITIVE 383 60 17 136 88 aact:AA02135.1|3end_hypothetical_protein_1459083..1459233_POSITIVE 382 106 62 52 4 aact:AA01986|3end_pyruvate_dehydrogenase_complex_E2_1350500..1350650_POSITIVE 372 100 64 47 14 aact:AA00453|3end_phosphoglyceromutase_318009..318159_POSITIVE 361 92 61 39 8 aact:AA01023|3end_conserved_hypothetical_protein_716435..716585_POSITIVE 359 109 63 106 60 aact:AA00384|3end_conserved_hypothetical_protein_(possible_nitrogen_fixation_protein)_261168..261318_POSITIVE 357 95 56 68 26 aact:AA02275|3end_conserved_hypothetical_protein_1549955..1550105_POSITIVE 357 107 64 86 46 aact:AA02246|3end_peptidase_E_1528355..1528505_POSITIVE 355 58 11 88 43 aact:AA01146|3end_conserved_hypothetical_protein_784770..784920_POSITIVE