Origin IGS: taatttttaatctaaaagagcggtcaaaattcatagtgttttgaccgctcttttacataggatataaacaccatattccatcaaaaataacttgacagctagattttaaaaggaaaaaaatatgcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgcagtttgaattttttgaggaagtgtggtttcaagaaaggtggcaatttccgcagcgcgggaatttaaaatgcgataaataccgaaatccagctccggttgattaatttgaaaaatttccttgagtttagatataagttgtgcaaggtgaggtgaggtgaggcatatttttttccttttaaaatctagctgtcaagttatttttgatggaatatggtgtttatatcctatgtaaaagagcggtcaaaacactatgaattttgaccgctcttttagattaaaaatta Mask Tandem Repeat Region ================================================ taatttttaatctaaaagagcggtcaaaattcatagtgttttgaccgctcttttacataggatataaacaccatattccatcaaaaataacttgacagctagattttaaaaggaaaaaaatatgcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgca Find is-nt database================================================ Query_seq: AA01922:AA01923|AA01922:AA01923:translation initiation factor IF2:type III restriction-modification system methylation subunit:->->:1297340..1297626 287 taatttttaatctaaaagagcggtcaaaattcatagtgttttgaccgctcttttacataggatataaacaccatattccatcaaaaataacttgacagctagattttaaaaggaaaaaaatatgcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01922:AA01923|AA01922:AA01923:translation initiation factor IF2:type III restriction-modification system methylation subunit:->->:1297340..1297626 287 taatttttaatctaaaagagcggtcaaaattcatagtgttttgaccgctcttttacataggatataaacaccatattccatcaaaaataacttgacagctagattttaaaaggaaaaaaatatgcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01922:AA01923|AA01922:AA01923:translation initiation factor IF2:type III restriction-modification system methylation subunit:->->:1297340..1297626 287 taatttttaatctaaaagagcggtcaaaattcatagtgttttgaccgctcttttacataggatataaacaccatattccatcaaaaataacttgacagctagattttaaaaggaaaaaaatatgcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 4 Min: 132 Max: 287 Len: 156 Subject: UniRef90_Q2DRK1 Cluster: Probable methylation subunit, type III restriction-modification system; n=1; Geobacter uraniumreducens Rf4|Rep: Probable methylation subunit, type III restriction-modification system - Geobacter uraniumreducens Rf4 HSP 1 e-value: 8.0E-9 bit: 61.6 Len: 138 Query Start:150 Query End:287 Subject Strand: null Subject Start: 42 Subject End: 87 ..................................................................................................................................................... L I S K L K E I F Q I N Q P E L D F G I Y R I L N S R A A E I A T F L E T T L P Q K I Q T A ..................................................................................................................................................... L V R K L K E I F Q I D R P E L D F G V Y R I L N A R A G E I N D Y L D N R L K A R V Q E A Subject: UniRef90_A3N069 Cluster: Putative methylation subunit, type III restriction-modification system; n=3; Actinobacillus pleuropneumoniae|Rep: Putative methylation subunit, type III restriction-modification system - Actinobacillus pleuropneumoniae serotype 5b (strain L20) HSP 1 e-value: 4.0E-8 bit: 59.3 Len: 126 Query Start:162 Query End:287 Subject Strand: null Subject Start: 1 Subject End: 42 ................................................................................................................................................................. L K E I F Q I N Q P E L D F G I Y R I L N S R A A E I A T F L E T T L P Q K I Q T A ................................................................................................................................................................. M Q E I F Q I N Q P D L D F G I Y R I L N S R Q N E I Q Q F L T R T L P N N I N G A Subject: UniRef90_Q5P888 Cluster: Probable methylation subunit, type III restriction-modification system; n=1; Azoarcus sp. EbN1|Rep: Probable methylation subunit, type III restriction-modification system - Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) HSP 1 e-value: 9.0E-8 bit: 58.2 Len: 147 Query Start:132 Query End:278 Subject Strand: null Subject Start: 4 Subject End: 52 ................................................................................................................................... S P H L A Q L I S K L K E I F Q I N Q P E L D F G I Y R I L N S R A A E I A T F L E T T L P Q K I ......... ................................................................................................................................... S K H K E R F I E L L N E L F Q L N Q P E L D F G L Y R I L H A R S A Q I K A F I D T E L G A E I ......... Subject: UniRef90_Q0A8E9 Cluster: DNA methylase N-4/N-6 domain protein; n=1; Alkalilimnicola ehrlichei MLHE-1|Rep: DNA methylase N-4/N-6 domain protein - Alkalilimnicola ehrlichei (strain MLHE-1) HSP 1 e-value: 6.0E-7 bit: 55.5 Len: 156 Query Start:132 Query End:287 Subject Strand: null Subject Start: 4 Subject End: 55 ................................................................................................................................... S P H L A Q L I S K L K E I F Q I N Q P E L D F G I Y R I L N S R A A E I A T F L E T T L P Q K I Q T A ................................................................................................................................... N P A F D K L I R L L K E L F Q L D Q P D L D F G L Y R I M H A R A D E I S Q F L D R D L L P Q V K D A taatttttaatctaaaagagcggtcaaaattcatagtgttttgaccgctcttttacataggatataaacaccatattccatcaaaaataacttgacagctagattttaaaaggaaaaaaatatgcctcaccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: + Start: 103 End: 219 ...................................................................................................... M L K G K K Y A S P H L T L H N L Y L N S R K F F K L I N R S W I S V F I A F .................................................................... Protein_Len: 56 Strand: + Start: 120 End: 287 ....................................................................................................................... M C L T S P H L A Q L I S K L K E I F Q I N Q P E L D F G I Y R I L N S R A A E I A T F L E T T L P Q K I Q T A taatttttaatctaaaagagcggtcaaaattcatagtgttttgaccgctcttttacataggatataaacaccatattccatcaaaaataacttgacagctagattttaaaaggaaaaaaatatgcctcaccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatttttaatctaaaagagcggtcaaaattcatagtgttttgaccgctcttttacataggatataaacaccatattccatcaaaaataacttgacagctagattttaaaaggaaaaaaatatgcctcaccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -13.5 Tail_Score: -4.27584 Start: 18 End: 50 Full_Region: atatcctatgtaaaa gagcggtcaaaacac tat gaattttgaccgctc ttttagattaaaaat .................gagcggtcaaaacactatgaattttgaccgctc............................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 47 HP_score: -2.2 Tail_Score: -3.56864 Start: 189 End: 205 Full_Region: TTTCAAATTAATCAA CCGGAG CTGGA TTTCGG TATTTATCGCATTTT ............................................................................................................................................................................................CCGGAGCTGGATTTCGG.................................................................................. TransTerm Strand: - Conf: 77 HP_score: -8.7 Tail_Score: -3.60627 Start: 224 End: 240 Full_Region: caagaaaggtggcaa tttccgc agc gcgggaa tttaaaatgcgataa ...............................................................................................................................................................................................................................tttccgcagcgcgggaa............................................... Find igs database================================================ Query_seq: AA01922:AA01923|AA01922:AA01923:translation initiation factor IF2:type III restriction-modification system methylation subunit:->->:1297340..1297626 287 taatttttaatctaaaagagcggtcaaaattcatagtgttttgaccgctcttttacataggatataaacaccatattccatcaaaaataacttgacagctagattttaaaaggaaaaaaatatgcctcaccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn Intra-Species Hit: Count: 1 Min: 1 Max: 131 Len: 131 Subject: aact_AA01922_AA01923|translation initiation factor IF2:type III restriction-modification system methylation subunit|POSITIVE:POSITIVE|[1297340,1297626]|287 HSP 1 e-value: 2.0E-68 bit: 260.0 Len: 131 Query Start:1 Query End:131 Subject Strand: POSITIVE Subject Start: 1 Subject End: 131 taatttttaatctaaaagagcggtcaaaattcatagtgttttgaccgctcttttacataggatataaacaccatattccatcaaaaataacttgacagctagattttaaaaggaaaaaaatatgcctcacc............................................................................................................................................................ taatttttaatctaaaagagcggtcaaaattcatagtgttttgaccgctcttttacataggatataaacaccatattccatcaaaaataacttgacagctagattttaaaaggaaaaaaatatgcctcacc............................................................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUUUUAAUCUAAAAGAGCGGUCAAAAUUCAUAGUGUUUUGACCGCUCUUUUACAUAGGAUAUAAACACCAUAUUCCAUCAAAAAUAACUUGACAGCUAGAUUUUAAAAGGAAAAAAAUAUGCCUCACC ..((((((....((((((((((((((((((.......))))))))))))))))))....(((..............)))...))))))....(((..((...(((((.........)))))..)).))).. (-30.24) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGUGAGGCAUAUUUUUUUCCUUUUAAAAUCUAGCUGUCAAGUUAUUUUUGAUGGAAUAUGGUGUUUAUAUCCUAUGUAAAAGAGCGGUCAAAACACUAUGAAUUUUGACCGCUCUUUUAGAUUAAAAAUUA ((..((((((......((((..((((((..(((((....))))).)))))).))))....))))))....)).((.(((((((((((((((((.........))))))))))))))))).))......... (-39.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 441 54 15 206 165 aact:AA02754|5end_conserved_hypothetical_protein_1928949..1929179_POSITIVE 431 53 14 149 108 aact:AA02010|5end_peptide_methionine_sulfoxide_reductase;_cytochrome_C-TYPE_biogenesis_protein;_transcriptional_regulator_1367180..1367410_POSITIVE 413 59 14 147 100 aact:AA00126|5end_conserved_hypothetical_protein_91053..91283_POSITIVE 404 48 12 157 118 aact:AA00625|5end_hypothetical_protein_430565..430795_POSITIVE 404 48 12 49 10 aact:AA00623|5end_hypothetical_protein_430457..430687_POSITIVE 401 54 11 159 114 aact:AA00759|5end_3-oxoacyl-[acyl-carrier-protein]_synthase_I_520603..520833_POSITIVE 376 54 16 41 1 aact:AA00758|5end_hypothetical_protein_520485..520715_POSITIVE 362 53 14 150 109 aact:AA02084|5end_conserved_hypothetical_protein_1417843..1418073_POSITIVE 348 54 14 153 111 aact:AA00124|5end_conserved_hypothetical_protein_(possible_cytoplasmic_protein)_90270..90500_POSITIVE 348 54 14 104 62 aact:AA00123|5end_hypothetical_protein_90221..90451_POSITIVE 348 54 14 95 53 aact:AA00122|5end_hypothetical_protein_90212..90442_POSITIVE 347 54 15 189 146 aact:AA02893|5end_oligopeptide_transport_system_permease_protein,_N-terminal_2027201..2027431_POSITIVE 346 48 1 60 6 aact:AA01877|5end_conserved_hypothetical_protein_1264115..1264345_POSITIVE 335 53 14 156 115 aact:AA00473|5end_tryptophanyl-tRNA_synthetase_328996..329226_POSITIVE 333 54 12 157 113 aact:AA02993|5end_6-phosphofructokinase_2091766..2091996_POSITIVE 330 53 14 201 158 aact:AA01830|5end_2,3-cyclic-nucleotide_2-phosphodiesterase_1231556..1231786_POSITIVE 330 53 14 48 5 aact:AA01829|5end_hypothetical_protein_1231403..1231633_POSITIVE 326 57 15 193 148 aact:AA01965|5end_poly(A)_polymerase_1329478..1329708_POSITIVE 324 54 14 195 151 aact:AA02902|5end_aerobic_respiration_control_protein_2031171..2031401_POSITIVE 316 57 15 164 114 aact:AA02113|5end_conserved_hypothetical_protein_1442183..1442413_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 459 55 13 78 36 aact:AA01922|3end_translation_initiation_factor_IF2_1297279..1297429_POSITIVE 441 54 15 45 4 aact:AA02751|3end_L-lactate_permease_1928868..1929018_POSITIVE 417 54 14 87 45 aact:AA02280|3end_conserved_hypothetical_protein_1552767..1552917_POSITIVE 404 48 12 144 105 aact:AA00623|3end_hypothetical_protein_430632..430782_POSITIVE 362 53 14 75 34 aact:AA02082|3end_glutaminyl-tRNA_synthetase_1417848..1417998_POSITIVE 348 54 14 84 42 aact:AA00120|3end_ATP-dependent_Clp_protease_ATP-binding_subunit_90281..90431_POSITIVE 347 54 15 85 42 aact:AA02892|3end_oligopeptide_ABC_transporter,_periplasmic-binding_protein_2027177..2027327_POSITIVE 335 53 14 63 22 aact:AA00471|3end_3,4-dihydroxy-2-butanone_4-phosphate_synthase;_GTP_cyclohydrase_II_328983..329133_POSITIVE 333 54 12 51 7 aact:AA02992|3end_RNA_methyltransferase_2091740..2091890_POSITIVE 326 57 15 73 28 aact:AA01963|3end_dnaK_suppressor_protein_1329438..1329588_POSITIVE 325 53 12 57 14 aact:AA00010|3end_transposase_orfB_fragment_6297..6447_POSITIVE 324 54 14 82 38 aact:AA02899|3end_oligopeptide_ABC_transporter,_ATP-binding_protein_2031138..2031288_POSITIVE 316 57 15 85 35 aact:AA02112|3end_cell_division_protein_1442184..1442334_POSITIVE 315 54 14 79 37 aact:AA02781|3end_protein-export_membrane_protein_1945981..1946131_POSITIVE 315 54 14 82 40 aact:AA02360|3end_threonine_synthase_1619947..1620097_POSITIVE 307 54 20 77 39 aact:AA01351|3end_probable_sulfatase_916393..916543_POSITIVE 303 54 12 78 34 aact:AA00896|3end_prolyl-tRNA_synthetase_(proline-tRNA_ligase)_623206..623356_POSITIVE 296 54 15 83 42 aact:AA02474|3end_transmembrane_protein_1711548..1711698_POSITIVE 287 53 14 76 36 aact:AA01083|3end_preprotein_translocase_SecA_subunit_750970..751120_POSITIVE 285 54 15 81 40 aact:AA02338|3end_conserved_hypothetical_protein;_possible_integral_membrane_protein_1598885..1599035_POSITIVE