Origin IGS: taactcaattgttgatgaattattagcaaaactcacttgcgcagagcttaaatgttacttggttgttgtgcgcaaaacaaagggttggaacaaagaataggacagtatttctgttagtcaattt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaattgactaacagaaatactgtcctattctttgttccaaccctttgttttgcgcacaacaaccaagtaacatttaagctctgcgcaagtgagttttgctaataattcatcaacaattgagtta Mask Tandem Repeat Region ================================================ taactcaattgttgatgaattattagcaaaactcacttgcgcagagcttaaatgttacttggttgttgtgcgcaaaacaaagggttggaacaaagaataggacagtatttctgttagtcaattt Find is-nt database================================================ Query_seq: AA01909:AA01911|AA01909:AA01911:hypothetical protein:hypothetical protein:->->:1287807..1287930 124 taactcaattgttgatgaattattagcaaaactcacttgcgcagagcttaaatgttacttggttgttgtgcgcaaaacaaagggttggaacaaagaataggacagtatttctgttagtcaattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01909:AA01911|AA01909:AA01911:hypothetical protein:hypothetical protein:->->:1287807..1287930 124 taactcaattgttgatgaattattagcaaaactcacttgcgcagagcttaaatgttacttggttgttgtgcgcaaaacaaagggttggaacaaagaataggacagtatttctgttagtcaattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01909:AA01911|AA01909:AA01911:hypothetical protein:hypothetical protein:->->:1287807..1287930 124 taactcaattgttgatgaattattagcaaaactcacttgcgcagagcttaaatgttacttggttgttgtgcgcaaaacaaagggttggaacaaagaataggacagtatttctgttagtcaattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taactcaattgttgatgaattattagcaaaactcacttgcgcagagcttaaatgttacttggttgttgtgcgcaaaacaaagggttggaacaaagaataggacagtatttctgttagtcaattt Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 8 End: 97 ....... M V D E L L A K L T C A E L K C Y L V V V R K T K G W N K E ........................... Protein_Len: 32 Strand: - Start: 26 End: 121 ......................... C F E S A C L K F T V Q N N H A F C L T P V F F L V T N R N T M ... taactcaattgttgatgaattattagcaaaactcacttgcgcagagcttaaatgttacttggttgttgtgcgcaaaacaaagggttggaacaaagaataggacagtatttctgttagtcaattt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taactcaattgttgatgaattattagcaaaactcacttgcgcagagcttaaatgttacttggttgttgtgcgcaaaacaaagggttggaacaaagaataggacagtatttctgttagtcaattt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01909:AA01911|AA01909:AA01911:hypothetical protein:hypothetical protein:->->:1287807..1287930 124 taactcaattgttgatgaattattagcaaaactcacttgcgcagagcttaaatgttacttggttgttgtgcgcaaaacaaagggttggaacaaagaataggacagtatttctgttagtcaattt Intra-Species Hit: Count: 1 Min: 1 Max: 124 Len: 124 Subject: aact_AA01909_AA01911|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1287807,1287930]|124 HSP 1 e-value: 1.0E-64 bit: 246.0 Len: 124 Query Start:1 Query End:124 Subject Strand: POSITIVE Subject Start: 1 Subject End: 124 taactcaattgttgatgaattattagcaaaactcacttgcgcagagcttaaatgttacttggttgttgtgcgcaaaacaaagggttggaacaaagaataggacagtatttctgttagtcaattt taactcaattgttgatgaattattagcaaaactcacttgcgcagagcttaaatgttacttggttgttgtgcgcaaaacaaagggttggaacaaagaataggacagtatttctgttagtcaattt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACUCAAUUGUUGAUGAAUUAUUAGCAAAACUCACUUGCGCAGAGCUUAAAUGUUACUUGGUUGUUGUGCGCAAAACAAAGGGUUGGAACAAAGAAUAGGACAGUAUUUCUGUUAGUCAAUUU ((((((..(((((((((...))))))))).......(((((((.(((.................))).)))))))......)))))).......((....(((((.....)))))..))..... (-29.03) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAUUGACUAACAGAAAUACUGUCCUAUUCUUUGUUCCAACCCUUUGUUUUGCGCACAACAACCAAGUAACAUUUAAGCUCUGCGCAAGUGAGUUUUGCUAAUAAUUCAUCAACAAUUGAGUUA .........((((((((((......)))).)))))).(((..(((...((((((((.........................)))))))).)))..)))....(((((((........))))))) (-17.41) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 309 74 31 204 165 aact:AA01153|5end_sec-independent_protein_translocase_790166..790396_POSITIVE 309 77 32 77 26 aact:AA00776|5end_conserved_hypothetical_protein_533496..533726_POSITIVE 289 73 33 124 77 aact:AA02996|5end_probable_cation_transport_protein_2093369..2093599_POSITIVE 287 89 45 70 9 aact:AA02758|5end_3-phosphoshikimate_1-carboxyvinyltransferase_1931802..1932032_POSITIVE 281 73 34 163 106 aact:AA00244|5end_conserved_hypothetical_protein_(possible_deoxyribonuclease,_possible_hydrolase)_172043..172273_POSITIVE 276 86 50 199 151 aact:AA00723|5end_peptidase_B_(leucyl_aminopeptidase)_496457..496687_POSITIVE 276 88 41 177 137 aact:AA00551|5end_hypothetical_protein_377225..377455_POSITIVE 275 47 4 169 130 aact:AA00895|5end_hypothetical_protein_621285..621515_POSITIVE 271 89 41 192 143 aact:AA00841|5end_UDP-MurNAc-tripeptide_synthetase_581318..581548_POSITIVE 270 72 33 203 154 aact:AA02195|5end_oxaloacetate_decarboxylase_alpha_chain_1494501..1494731_POSITIVE 267 75 36 206 164 aact:AA02137|5end_transcriptional_regulators_(TetR/AcrR_family)_1460453..1460683_POSITIVE 264 72 37 181 143 aact:AA00480|5end_mannose-specific_phosphotransferase_system_IID_334286..334516_POSITIVE 262 72 37 190 155 aact:AA00832|5end_D-alanine-D-alanine_ligase_B_572769..572999_POSITIVE 260 72 36 179 143 aact:AA03004|5end_signal_peptidase_I_(SPase_I)_2098381..2098611_POSITIVE 260 72 36 50 13 aact:AA01360|5end_XAA-pro_aminopeptidase_(X-pro_aminopeptidase)_921047..921277_POSITIVE 259 86 41 162 118 aact:AA01904|5end_hypothetical_protein_1284448..1284678_POSITIVE 259 78 43 38 2 aact:AA01811|5end_electron_transport_complex_protein_1220336..1220566_POSITIVE 256 73 31 153 99 aact:AA02045|5end_PTS_system,_galactitol-specific_IIB_component_1391956..1392186_POSITIVE 256 65 33 229 195 aact:AA01476|5end_hypothetical_protein_991402..991632_POSITIVE 255 72 33 199 146 aact:AA02244|5end_conserved_hypothetical_protein_(probable_DNA-binding_protein;_death-on-curing_family_protein0_1526402..1526632_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 333 78 34 101 63 aact:AA02959|3end_pyrroline-5-carboxylate_(P5C)_reductase_2070976..2071126_POSITIVE 309 77 32 71 20 aact:AA00775|3end_conserved_hypothetical_protein_(possible_acyl_CoA_thioester_hydrolase)_533570..533720_POSITIVE 281 73 34 130 73 aact:AA00241|3end_formate_acetyltransferase_activating_enzyme_(anaerobic_ribonucleoside-triphosphate_reductase_activating_protein)_172090..172240_POSITIVE 276 88 41 73 33 aact:AA00550|3end_ferredoxin-type_protein_377201..377351_POSITIVE 267 75 36 149 107 aact:AA02136|3end_aspartate_aminotransferase_1460476..1460626_POSITIVE 266 70 23 137 97 aact:AA01262|3end_hydrolase_860087..860237_POSITIVE 262 88 59 147 105 aact:AA02758|3end_3-phosphoshikimate_1-carboxyvinyltransferase_1933150..1933300_POSITIVE 256 73 31 131 77 aact:AA02044|3end_phosphotransferase_system_enzyme_II,_galactitol_specific,_protein_A_1392014..1392164_POSITIVE 255 72 33 128 75 aact:AA02243|3end_conserved_hypothetical_protein_1526411..1526561_POSITIVE 254 75 38 117 80 aact:AA02606|3end_ABC_transporter,_ATP-binding_protein/permease_1818451..1818601_POSITIVE 253 44 4 147 115 aact:AA00894|3end_selenium_transferase_(Cysteinyl-tRNA(Sec)_selenium_transferase)_621350..621500_POSITIVE 251 90 51 48 6 aact:AA00839|3end_UDP-MurNAc-pentapeptide_phosphotransferase_579784..579934_POSITIVE 251 110 61 45 6 aact:AA00753|3end_galactoside_ABC_transporter,_permease_protein_518615..518765_POSITIVE 249 75 32 115 79 aact:AA01083|3end_preprotein_translocase_SecA_subunit_750970..751120_POSITIVE 248 90 41 81 32 aact:AA02821|3end_ribosomal-protein-alanine_acetyltransferase_1982775..1982925_POSITIVE 241 106 64 44 2 aact:AA02893|3end_oligopeptide_transport_system_permease_protein,_N-terminal_2027544..2027694_POSITIVE 239 88 41 48 7 aact:AA03012|3end_conserved_hypothetical_protein_2104556..2104706_POSITIVE 239 73 32 142 94 aact:AA00041|3end_cytochrome_c-type_biogenesis_protein_33817..33967_POSITIVE 238 97 53 102 49 aact:AA00367|3end_50S_ribosomal_protein_L23_247436..247586_POSITIVE 233 94 58 132 106 aact:AA02663|3end_DNA_ligase_1854351..1854501_POSITIVE