Origin IGS: taacctgtttcaaatttttaaagagcaactcaaagtggtttgctttgatgtggtgtattatgtacttatagttcattatagtcaagaacaaaaagtacacattttagataaaatgtactaaatgttcatatttaattgattttaaaagaaataaattttcaaaattttttgattaattgctgaatttgtgacctagatcacaaaaaaaagaggggggaattttaaaaagtgcggtaggaattgcgggcaaaagaaaaccgcccgaaggcggtttgtcggtatataaactgattaagatcctacatcgcaaaaaattccttgaggtgggttaaatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatttaacccacctcaaggaattttttgcgatgtaggatcttaatcagtttatataccgacaaaccgccttcgggcggttttcttttgcccgcaattcctaccgcactttttaaaattcccccctcttttttttgtgatctaggtcacaaattcagcaattaatcaaaaaattttgaaaatttatttcttttaaaatcaattaaatatgaacatttagtacattttatctaaaatgtgtactttttgttcttgactataatgaactataagtacataatacaccacatcaaagcaaaccactttgagttgctctttaaaaatttgaaacaggtta Mask Tandem Repeat Region ================================================ taacctgtttcaaatttttaaagagcaactcaaagtggtttgctttgatgtggtgtattatgtacttatagttcattatagtcaagaacaaaaagtacacattttagataaaatgtactaaatgttcatatttaattgattttaaaagaaataaattttcaaaattttttgattaattgctgaatttgtgacctagatcacaaaaaaaagaggggggaattttaaaaagtgcggtaggaattgcgggcaaaagaaaaccgcccgaaggcggtttgtcggtatataaactgattaagatcctacatcgcaaaaaattccttgaggtgggttaaatta Find is-nt database================================================ Query_seq: AA01904:AA01905|AA01904:AA01905:hypothetical protein:hypothetical protein:->->:1285110..1285445 336 taacctgtttcaaatttttaaagagcaactcaaagtggtttgctttgatgtggtgtattatgtacttatagttcattatagtcaagaacaaaaagtacacattttagataaaatgtactaaatgttcatatttaattgattttaaaagaaataaattttcaaaattttttgattaattgctgaatttgtgacctagatcacaaaaaaaagaggggggaattttaaaaagtgcggtaggaattgcgggcaaaagaaaaccgcccgaaggcggtttgtcggtatataaactgattaagatcctacatcgcaaaaaattccttgaggtgggttaaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01904:AA01905|AA01904:AA01905:hypothetical protein:hypothetical protein:->->:1285110..1285445 336 taacctgtttcaaatttttaaagagcaactcaaagtggtttgctttgatgtggtgtattatgtacttatagttcattatagtcaagaacaaaaagtacacattttagataaaatgtactaaatgttcatatttaattgattttaaaagaaataaattttcaaaattttttgattaattgctgaatttgtgacctagatcacaaaaaaaagaggggggaattttaaaaagtgcggtaggaattgcgggcaaaagaaaaccgcccgaaggcggtttgtcggtatataaactgattaagatcctacatcgcaaaaaattccttgaggtgggttaaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01904:AA01905|AA01904:AA01905:hypothetical protein:hypothetical protein:->->:1285110..1285445 336 taacctgtttcaaatttttaaagagcaactcaaagtggtttgctttgatgtggtgtattatgtacttatagttcattatagtcaagaacaaaaagtacacattttagataaaatgtactaaatgttcatatttaattgattttaaaagaaataaattttcaaaattttttgattaattgctgaatttgtgacctagatcacaaaaaaaagaggggggaattttaaaaagtgcggtaggaattgcgggcaaaagaaaaccgcccgaaggcggtttgtcggtatataaactgattaagatcctacatcgcaaaaaattccttgaggtgggttaaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taacctgtttcaaatttttaaagagcaactcaaagtggtttgctttgatgtggtgtattatgtacttatagttcattatagtcaagaacaaaaagtacacattttagataaaatgtactaaatgttcatatttaattgattttaaaagaaataaattttcaaaattttttgattaattgctgaatttgtgacctagatcacaaaaaaaagaggggggaattttaaaaagtgcggtaggaattgcgggcaaaagaaaaccgcccgaaggcggtttgtcggtatataaactgattaagatcctacatcgcaaaaaattccttgaggtgggttaaatta Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: + Start: 5 End: 118 .... M F Q I F K E Q L K V V C F D V V Y Y V L I V H Y S Q E Q K V H I L D K M Y .......................................................................................................................................................................................................................... Protein_Len: 37 Strand: + Start: 219 End: 329 .......................................................................................................................................................................................................................... M L K S A V G I A G K R K P P E G G L S V Y K L I K I L H R K K F L E V G ....... Protein_Len: 45 Strand: - Start: 176 End: 310 ............................................................................................................................................................................... N S F K H G L D C F F S P P I K F L A T P I A P L L F G G S P P K D T Y L S I L I R C R M .......................... Protein_Len: 33 Strand: - Start: 2 End: 100 . V Q K L N K F L L E F H N A K I H H I I Y K Y N M I T L F L F Y M ............................................................................................................................................................................................................................................ taacctgtttcaaatttttaaagagcaactcaaagtggtttgctttgatgtggtgtattatgtacttatagttcattatagtcaagaacaaaaagtacacattttagataaaatgtactaaatgttcatatttaattgattttaaaagaaataaattttcaaaattttttgattaattgctgaatttgtgacctagatcacaaaaaaaagaggggggaattttaaaaagtgcggtaggaattgcgggcaaaagaaaaccgcccgaaggcggtttgtcggtatataaactgattaagatcctacatcgcaaaaaattccttgaggtgggttaaatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 83 Start: 57 End: 86 ........................................................CTTGACTATAATGAACTATAAGTACATAAT.......................................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taacctgtttcaaatttttaaagagcaactcaaagtggtttgctttgatgtggtgtattatgtacttatagttcattatagtcaagaacaaaaagtacacattttagataaaatgtactaaatgttcatatttaattgattttaaaagaaataaattttcaaaattttttgattaattgctgaatttgtgacctagatcacaaaaaaaagaggggggaattttaaaaagtgcggtaggaattgcgggcaaaagaaaaccgcccgaaggcggtttgtcggtatataaactgattaagatcctacatcgcaaaaaattccttgaggtgggttaaatta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 42 HP_score: -3.2 Tail_Score: -2.67021 Start: 30 End: 48 Full_Region: acataatacaccaca tcaaag caaacca ctttga gttgctctttaaaaa .............................tcaaagcaaaccactttga................................................................................................................................................................................................................................................................................................ TransTerm Strand: - Conf: 100 HP_score: -9.5 Tail_Score: -5.07652 Start: 258 End: 271 Full_Region: ttatataccgacaaa ccgcc ttcg ggcgg ttttcttttgcccgc .................................................................................................................................................................................................................................................................ccgccttcgggcgg................................................................. Find igs database================================================ Query_seq: AA01904:AA01905|AA01904:AA01905:hypothetical protein:hypothetical protein:->->:1285110..1285445 336 taacctgtttcaaatttttaaagagcaactcaaagtggtttgctttgatgtggtgtattatgtacttatagttcattatagtcaagaacaaaaagtacacattttagataaaatgtactaaatgttcatatttaattgattttaaaagaaataaattttcaaaattttttgattaattgctgaatttgtgacctagatcacaaaaaaaagaggggggaattttaaaaagtgcggtaggaattgcgggcaaaagaaaaccgcccgaaggcggtttgtcggtatataaactgattaagatcctacatcgcaaaaaattccttgaggtgggttaaatta Intra-Species Hit: Count: 1 Min: 1 Max: 336 Len: 336 Subject: aact_AA01904_AA01905|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1285110,1285445]|336 HSP 1 e-value: 4.0E-67 bit: 256.0 Len: 129 Query Start:1 Query End:129 Subject Strand: POSITIVE Subject Start: 1 Subject End: 129 taacctgtttcaaatttttaaagagcaactcaaagtggtttgctttgatgtggtgtattatgtacttatagttcattatagtcaagaacaaaaagtacacattttagataaaatgtactaaatgttcat............................................................................................................................................................................................................... taacctgtttcaaatttttaaagagcaactcaaagtggtttgctttgatgtggtgtattatgtacttatagttcattatagtcaagaacaaaaagtacacattttagataaaatgtactaaatgttcat............................................................................................................................................................................................................... HSP 2 e-value: 7.0E-66 bit: 252.0 Len: 127 Query Start:210 Query End:336 Subject Strand: POSITIVE Subject Start: 210 Subject End: 336 .................................................................................................................................................................................................................gaggggggaattttaaaaagtgcggtaggaattgcgggcaaaagaaaaccgcccgaaggcggtttgtcggtatataaactgattaagatcctacatcgcaaaaaattccttgaggtgggttaaatta .................................................................................................................................................................................................................gaggggggaattttaaaaagtgcggtaggaattgcgggcaaaagaaaaccgcccgaaggcggtttgtcggtatataaactgattaagatcctacatcgcaaaaaattccttgaggtgggttaaatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACCUGUUUCAAAUUUUUAAAGAGCAACUCAAAGUGGUUUGCUUUGAUGUGGUGUAUUAUGUACUUAUAGUUCAUUAUAGUCAAGAACAAAAAGUACACAUUUUAGAUAAAAUGUACUAAAUGUUCAUAUUUAAUUGAUUUUAAAAGAAAUAAAUUUUCAAAAUUUUUUGAUUAAUUGCUGAAUUUGUGACCUAGAUCACAAAAAAAAGAGGGGGGAAUUUUAAAAAGUGCGGUAGGAAUUGCGGGCAAAAGAAAACCGCCCGAAGGCGGUUUGUCGGUAUAUAAACUGAUUAAGAUCCUACAUCGCAAAAAAUUCCUUGAGGUGGGUUAAAUUA (((((..(((((..(((((....(((((.(((((((.....)))))))(((((((.((((((....)))))).))))))).....(((((...((((((..((((....))))))))))...)))))....((((..((((((((((........)))).))))))..))))....)))))...(((((((......)))))))..)))))...(((((((((......((((((((((..(((.(((((......((((((....))))))))))).)))......((.....)).))))))..)))).))))))))))))))..)))))..... (-84.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUUAACCCACCUCAAGGAAUUUUUUGCGAUGUAGGAUCUUAAUCAGUUUAUAUACCGACAAACCGCCUUCGGGCGGUUUUCUUUUGCCCGCAAUUCCUACCGCACUUUUUAAAAUUCCCCCCUCUUUUUUUUGUGAUCUAGGUCACAAAUUCAGCAAUUAAUCAAAAAAUUUUGAAAAUUUAUUUCUUUUAAAAUCAAUUAAAUAUGAACAUUUAGUACAUUUUAUCUAAAAUGUGUACUUUUUGUUCUUGACUAUAAUGAACUAUAAGUACAUAAUACACCACAUCAAAGCAAACCACUUUGAGUUGCUCUUUAAAAAUUUGAAACAGGUUA .....(((((....((((((((((((.((((..((((((....................((.((((((((....))))))))))..(((....))).))))))))))......))))))))...........((((((((....))))))))...((((((...(((((....)))))..............((((((..(((((((......)))))))..)))))).......(((((((((...((((...........))))...)))))))))..........((((((.......))))))))))))..........))))....))))) (-72.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 402 272 231 207 165 aact:AA02337|5end_DNA_mismatch_repair_protein_1597350..1597580_POSITIVE 400 280 231 52 5 aact:AA02574|5end_dGTP_triphosphohydrolase_1792395..1792625_POSITIVE 382 289 243 210 161 aact:AA00339|5end_hypothetical_protein_228687..228917_POSITIVE 369 280 231 56 5 aact:AA00601|5end_hypothetical_protein_408863..409093_POSITIVE 359 277 242 191 155 aact:AA02581|5end_conserved_hypothetical_protein_1796966..1797196_POSITIVE 359 280 234 204 155 aact:AA00719|5end_hypothetical_protein_494627..494857_POSITIVE 358 280 231 92 37 aact:AA01900|5end_hypothetical_protein_1282233..1282463_POSITIVE 352 281 244 54 9 aact:AA02507|5end_penicillin-insensitive_murein_endopeptidase_A_1740058..1740288_POSITIVE 352 279 231 213 156 aact:AA00270|5end_malonyl_coA-acyl_carrier_protein_transacylase_189361..189591_POSITIVE 350 270 226 86 32 aact:AA00602|5end_1-deoxy-D-xylulose_5-phosphate_reductoisomerase_409077..409307_POSITIVE 349 280 231 191 128 aact:AA01270|5end_hydrolase_(possible_2-haloalkanoic_acid_dehalogenase_I)_863317..863547_POSITIVE 347 280 231 54 9 aact:AA01188|5end_conserved_hypothetical_protein_813912..814142_POSITIVE 347 276 231 60 19 aact:AA01099|5end_dimethyladenosine_transferase_758542..758772_POSITIVE 346 270 229 211 173 aact:AA00129|5end_hypothetical_protein_94122..94352_POSITIVE 340 279 233 214 178 aact:AA01313|5end_deoxyuridinetriphosphatase;_deoxyuridine_5'-triphosphate_nucleotidohydrolase_889567..889797_POSITIVE 339 248 211 121 79 aact:AA00467|5end_hypothetical_protein_324565..324795_POSITIVE 337 279 231 190 153 aact:AA00245|5end_hypothetical_protein_172834..173064_POSITIVE 336 279 232 178 118 aact:AA01083|5end_preprotein_translocase_SecA_subunit_748131..748361_POSITIVE 332 279 235 179 134 aact:AA00408|5end_SUN_protein_(FMU_protein)_272256..272486_POSITIVE 331 280 231 70 14 aact:AA02955|5end_trigger_factor_2067022..2067252_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 369 280 231 52 1 aact:AA00600|3end_ribosome_recycling_factor_(ribosome_releasing_factor)_408939..409089_POSITIVE 352 281 244 47 2 aact:AA02506|3end_possible_transmembrane_protein_1740131..1740281_POSITIVE 347 280 231 54 9 aact:AA01187|3end_conserved_hypothetical_protein_813992..814142_POSITIVE 342 280 237 145 109 aact:AA00585|3end_glycerol-3-phosphate_regulon_repressor_399778..399928_POSITIVE 340 280 232 141 103 aact:AA01778|3end_aminopeptidase_A/I;_cytosol_aminopeptidase;_leucyl_aminopeptidase_1199965..1200115_POSITIVE 339 248 211 76 34 aact:AA00465|3end_hypothetical_protein_324600..324750_POSITIVE 337 279 231 88 51 aact:AA00244|3end_conserved_hypothetical_protein_(possible_deoxyribonuclease,_possible_hydrolase)_172812..172962_POSITIVE 331 269 226 135 100 aact:AA02862|3end_hypothetical_protein_2008848..2008998_POSITIVE 330 277 231 61 15 aact:AA02967|3end_elongation_factor_P_(EF-P)_2075914..2076064_POSITIVE 330 280 231 46 1 aact:AA02700|3end_succinyl-CoA_synthase,_alpha_subunit_1884205..1884355_POSITIVE 330 287 241 142 91 aact:AA02014|3end_probable_hemolysin_1370977..1371127_POSITIVE 328 242 209 52 11 aact:AA02358|3end_homoserine_kinase_1618149..1618299_POSITIVE 327 285 243 110 56 aact:AA01875|3end_PTS_system,_glucose-specific_enzyme_II,_A_component_1263506..1263656_POSITIVE 319 278 231 135 87 aact:AA00744|3end_aldose_1-epimerase_(mutarotase)_511246..511396_POSITIVE 315 261 225 76 40 aact:AA00191|3end_conserved_hypothetical_protein_(possible_acetyltransferase,_GNAT_family)_135545..135695_POSITIVE 313 276 232 41 1 aact:AA01098|3end_bis(5'-nucleosyl)-tetraphosphatase_758603..758753_POSITIVE 312 268 222 84 44 aact:AA02117|3end_probable_transposase_1445793..1445943_POSITIVE 312 280 233 86 21 aact:AA00382|3end_hypothetical_protein_258885..259035_POSITIVE 311 278 231 150 77 aact:AA02226|3end_ABC_transporter_ATP-binding_protein_1514806..1514956_POSITIVE 311 279 233 79 38 aact:AA01885|3end_hydrogenase_nickel_incorporation_protein_1271535..1271685_POSITIVE