Origin IGS: gatgttctcccgatgttttgtttgaatgggccgcattctagcatattttgccgagggcggttgtgcaggcgattttttataggaacttttaattgagggaaagagtcaaaaatatgccttttcggcaaggaacaaaataactgttaagtaacgattaaacccgggagaaattaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttaatttctcccgggtttaatcgttacttaacagttattttgttccttgccgaaaaggcatatttttgactctttccctcaattaaaagttcctataaaaaatcgcctgcacaaccgccctcggcaaaatatgctagaatgcggcccattcaaacaaaacatcgggagaacatc Mask Tandem Repeat Region ================================================ gatgttctcccgatgttttgtttgaatgggccgcattctagcatattttgccgagggcggttgtgcaggcgattttttataggaacttttaattgagggaaagagtcaaaaatatgccttttcggcaaggaacaaaataactgttaagtaacgattaaacccgggagaaattaaa Find is-nt database================================================ Query_seq: AA01873:AA01874|AA01873:AA01874:mannose-6-phosphate isomerase:conserved hypothetical protein:->->:1262001..1262175 175 gatgttctcccgatgttttgtttgaatgggccgcattctagcatattttgccgagggcggttgtgcaggcgattttttataggaacttttaattgagggaaagagtcaaaaatatgccttttcggcaaggaacaaaataactgttaagtaacgattaaacccgggagaaattaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01873:AA01874|AA01873:AA01874:mannose-6-phosphate isomerase:conserved hypothetical protein:->->:1262001..1262175 175 gatgttctcccgatgttttgtttgaatgggccgcattctagcatattttgccgagggcggttgtgcaggcgattttttataggaacttttaattgagggaaagagtcaaaaatatgccttttcggcaaggaacaaaataactgttaagtaacgattaaacccgggagaaattaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01873:AA01874|AA01873:AA01874:mannose-6-phosphate isomerase:conserved hypothetical protein:->->:1262001..1262175 175 gatgttctcccgatgttttgtttgaatgggccgcattctagcatattttgccgagggcggttgtgcaggcgattttttataggaacttttaattgagggaaagagtcaaaaatatgccttttcggcaaggaacaaaataactgttaagtaacgattaaacccgggagaaattaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gatgttctcccgatgttttgtttgaatgggccgcattctagcatattttgccgagggcggttgtgcaggcgattttttataggaacttttaattgagggaaagagtcaaaaatatgccttttcggcaaggaacaaaataactgttaagtaacgattaaacccgggagaaattaaa Predict ORF larger than 30AA ================================================ Protein_Len: 42 Strand: - Start: 3 End: 128 .. T R G I N Q K F P G C E L M N Q R P R N H L R N K I P V K L Q P F S D F I H R K P M ............................................... Protein_Len: 31 Strand: - Start: 1 End: 93 I N E R H K T Q I P R M R A Y K A S P P Q A P S K K Y S S K M .................................................................................. gatgttctcccgatgttttgtttgaatgggccgcattctagcatattttgccgagggcggttgtgcaggcgattttttataggaacttttaattgagggaaagagtcaaaaatatgccttttcggcaaggaacaaaataactgttaagtaacgattaaacccgggagaaattaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gatgttctcccgatgttttgtttgaatgggccgcattctagcatattttgccgagggcggttgtgcaggcgattttttataggaacttttaattgagggaaagagtcaaaaatatgccttttcggcaaggaacaaaataactgttaagtaacgattaaacccgggagaaattaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01873:AA01874|AA01873:AA01874:mannose-6-phosphate isomerase:conserved hypothetical protein:->->:1262001..1262175 175 gatgttctcccgatgttttgtttgaatgggccgcattctagcatattttgccgagggcggttgtgcaggcgattttttataggaacttttaattgagggaaagagtcaaaaatatgccttttcggcaaggaacaaaataactgttaagtaacgattaaacccgggagaaattaaa Intra-Species Hit: Count: 1 Min: 1 Max: 175 Len: 175 Subject: aact_AA01873_AA01874|mannose-6-phosphate isomerase:conserved hypothetical protein|POSITIVE:POSITIVE|[1262001,1262175]|175 HSP 1 e-value: 8.0E-95 bit: 347.0 Len: 175 Query Start:1 Query End:175 Subject Strand: POSITIVE Subject Start: 1 Subject End: 175 gatgttctcccgatgttttgtttgaatgggccgcattctagcatattttgccgagggcggttgtgcaggcgattttttataggaacttttaattgagggaaagagtcaaaaatatgccttttcggcaaggaacaaaataactgttaagtaacgattaaacccgggagaaattaaa gatgttctcccgatgttttgtttgaatgggccgcattctagcatattttgccgagggcggttgtgcaggcgattttttataggaacttttaattgagggaaagagtcaaaaatatgccttttcggcaaggaacaaaataactgttaagtaacgattaaacccgggagaaattaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUGUUCUCCCGAUGUUUUGUUUGAAUGGGCCGCAUUCUAGCAUAUUUUGCCGAGGGCGGUUGUGCAGGCGAUUUUUUAUAGGAACUUUUAAUUGAGGGAAAGAGUCAAAAAUAUGCCUUUUCGGCAAGGAACAAAAUAACUGUUAAGUAACGAUUAAACCCGGGAGAAAUUAAA ....((((((((.((((((((((......((((.......(((((..(((((...))))).))))).((((((((((....((..(((((........)))))..)))))))).))))....))))...))))))))))..((((....))))........))))))))...... (-45.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUAAUUUCUCCCGGGUUUAAUCGUUACUUAACAGUUAUUUUGUUCCUUGCCGAAAAGGCAUAUUUUUGACUCUUUCCCUCAAUUAAAAGUUCCUAUAAAAAAUCGCCUGCACAACCGCCCUCGGCAAAAUAUGCUAGAAUGCGGCCCAUUCAAACAAAACAUCGGGAGAACAUC ......(((((((((((((...........(((((.....)))))....((((....(((((((((((((.........))))......................(((.((......))....))).)))))))))......))))...........)))).))))))))).... (-36.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 408 71 31 108 71 aact:AA00500|5end_hypothetical_protein_347151..347381_POSITIVE 383 69 24 170 123 aact:AA01519|5end_conserved_hypothetical_protein_1021794..1022024_POSITIVE 375 71 31 159 105 aact:AA01418|5end_conserved_hypothetical_protein_953951..954181_POSITIVE 373 79 32 173 109 aact:AA01003|5end_conserved_hypothetical_protein_700120..700350_POSITIVE 370 73 31 64 25 aact:AA00463|5end_class_B_acid_phosphatase_323114..323344_POSITIVE 364 70 22 204 155 aact:AA00161|5end_pilin_B_biogenesis_protein;_fimbrial_transcription_regulation_113384..113614_POSITIVE 359 69 21 184 120 aact:AA02034|5end_ferredoxin_NADP+_reductase_1385198..1385428_POSITIVE 359 69 21 66 2 aact:AA02032|5end_hypothetical_protein_1385080..1385310_POSITIVE 357 72 31 138 91 aact:AA01866|5end_thiamin_ABC_transporter,_permease_protein_1253313..1253543_POSITIVE 355 69 24 86 24 aact:AA00717|5end_methionyl-tRNA_synthetase_491304..491534_POSITIVE 349 69 21 81 8 aact:AA01151|5end_porphobilinogen_synthase_(delta-aminolevulinic_acid_dehydratase)_788323..788553_POSITIVE 349 70 29 161 125 aact:AA00993|5end_possible_D-tyrosyl-tRNA(Tyr)_deacylase_694488..694718_POSITIVE 344 83 41 48 3 aact:AA01209|5end_conserved_hypothetical_protein_826430..826660_POSITIVE 338 71 31 130 73 aact:AA00545|5end_glutathionylspermidine_synthetase;amidase_373607..373837_POSITIVE 337 72 32 231 192 aact:AA02575|5end_transmembrane_protein_1793749..1793979_POSITIVE 336 67 21 172 128 aact:AA01976|5end_colicin_V_production_protein_1336513..1336743_POSITIVE 336 69 27 203 155 aact:AA01760|5end_hydrogenase_isoenzymes_formation_protein_1189787..1190017_POSITIVE 332 78 31 224 174 aact:AA02972|5end_conserved_hypothetical_protein_(tyrosine_phenol_lyase-related_protein)_2077381..2077611_POSITIVE 331 74 33 49 9 aact:AA02509|5end_chorismate_synthase_1740952..1741182_POSITIVE 329 67 29 224 183 aact:AA00460|5end_ATP-dependent_ClpY-related_protease;_heat_shock_protein_321023..321253_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 383 69 24 87 40 aact:AA01517|3end_peptidyl-prolyl_cis-trans_isomerase;_macrophage_infectivity_protein_1021791..1021941_POSITIVE 366 69 27 144 101 aact:AA02100|3end_lipoprotein_1430759..1430909_POSITIVE 357 72 31 54 7 aact:AA01865|3end_thiamin_ABC_transporter,_periplasmic-binding_protein_1253309..1253459_POSITIVE 334 72 31 145 113 aact:AA01262|3end_hydrolase_860087..860237_POSITIVE 327 58 11 135 78 aact:AA02970|3end_tyrosine_phenol-lyase_2077391..2077541_POSITIVE 327 79 47 129 90 aact:AA01384|3end_hypothetical_protein_935540..935690_POSITIVE 326 84 41 93 55 aact:AA00741|3end_possible_2-oxoglutarate/malate_transporter_508076..508226_POSITIVE 325 69 21 66 15 aact:AA00451|3end_hypothetical_protein_315348..315498_POSITIVE 324 60 11 60 18 aact:AA01314|3end_hypothetical_protein_890196..890346_POSITIVE 324 71 32 58 19 aact:AA01064|3end_conserved_hypothetical_protein_(possible_aminomethyltransferase)_738392..738542_POSITIVE 321 69 25 61 1 aact:AA02031|3end_type_III_site-specific_deoxyribonuclease_1385155..1385305_POSITIVE 319 59 12 65 20 aact:AA01018|3end_conserved_hypothetical_protein_(possible_transcriptional_regulator)_712300..712450_POSITIVE 316 70 26 136 82 aact:AA00041|3end_cytochrome_c-type_biogenesis_protein_33817..33967_POSITIVE 315 70 31 138 73 aact:AA01145|3end_glycyl-tRNA_synthetase_alpha_subunit_784460..784610_POSITIVE 314 60 11 149 97 aact:AA02443|3end_cytochrome_C_related_protein_1686590..1686740_POSITIVE 311 73 31 135 93 aact:AA02928|3end_conserved_hypothetical_protein_2049244..2049394_POSITIVE 309 90 46 134 96 aact:AA02758|3end_3-phosphoshikimate_1-carboxyvinyltransferase_1933150..1933300_POSITIVE 304 60 11 109 66 aact:AA02157|3end_TonB-dependent_outer_membrane_receptor_1476305..1476455_POSITIVE 302 96 55 84 52 aact:AA02560|3end_integral_membrane_protein_1783630..1783780_POSITIVE 302 70 30 55 17 aact:AA01709|3end_glutathione-S-transferase-nitroreductase_A_fusion_protein;_modulator_of_drug_activity_A_1159334..1159484_POSITIVE