Origin IGS: taaatgtgttctccaataaacattaacatactcgaaatttccatgcgatttcggtaactgctatgacaaaaaaaatccttgaatgttcggtgggttgtacggcttttcagataaaaaataaaaaagtgcggtcactctggaaatcaaattttatgaccgcacttatattggtttttagcttaataaataacta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagttatttattaagctaaaaaccaatataagtgcggtcataaaatttgatttccagagtgaccgcacttttttattttttatctgaaaagccgtacaacccaccgaacattcaaggattttttttgtcatagcagttaccgaaatcgcatggaaatttcgagtatgttaatgtttattggagaacacattta Mask Tandem Repeat Region ================================================ taaatgtgttctccaataaacattaacatactcgaaatttccatgcgatttcggtaactgctatgacaaaaaaaatccttgaatgttcggtgggttgtacggcttttcagataaaaaataaaaaagtgcggtcactctggaaatcaaattttatgaccgcacttatattggtttttagcttaataaataacta Find is-nt database================================================ Query_seq: AA01869:AA01871|AA01869:AA01871:periplasmic serine protease do; hhoA-like precursor; heat shock protein:conserved hypothetical protein:->->:1258170..1258362 193 taaatgtgttctccaataaacattaacatactcgaaatttccatgcgatttcggtaactgctatgacaaaaaaaatccttgaatgttcggtgggttgtacggcttttcagataaaaaataaaaaagtgcggtcactctggaaatcaaattttatgaccgcacttatattggtttttagcttaataaataacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01869:AA01871|AA01869:AA01871:periplasmic serine protease do; hhoA-like precursor; heat shock protein:conserved hypothetical protein:->->:1258170..1258362 193 taaatgtgttctccaataaacattaacatactcgaaatttccatgcgatttcggtaactgctatgacaaaaaaaatccttgaatgttcggtgggttgtacggcttttcagataaaaaataaaaaagtgcggtcactctggaaatcaaattttatgaccgcacttatattggtttttagcttaataaataacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01869:AA01871|AA01869:AA01871:periplasmic serine protease do; hhoA-like precursor; heat shock protein:conserved hypothetical protein:->->:1258170..1258362 193 taaatgtgttctccaataaacattaacatactcgaaatttccatgcgatttcggtaactgctatgacaaaaaaaatccttgaatgttcggtgggttgtacggcttttcagataaaaaataaaaaagtgcggtcactctggaaatcaaattttatgaccgcacttatattggtttttagcttaataaataacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaatgtgttctccaataaacattaacatactcgaaatttccatgcgatttcggtaactgctatgacaaaaaaaatccttgaatgttcggtgggttgtacggcttttcagataaaaaataaaaaagtgcggtcactctggaaatcaaattttatgaccgcacttatattggtttttagcttaataaataacta Predict ORF larger than 30AA ================================================ Protein_Len: 48 Strand: + Start: 48 End: 191 ............................................... M S V T A M T K K I L E C S V G C T A F Q I K N K K V R S L W K S N F M T A L I L V F S L I N N .. Protein_Len: 39 Strand: - Start: 3 End: 119 .. I H E G I F M L M S S I E M R N R Y S S H C F F D K F T R H T T R S K L Y F M .......................................................................... taaatgtgttctccaataaacattaacatactcgaaatttccatgcgatttcggtaactgctatgacaaaaaaaatccttgaatgttcggtgggttgtacggcttttcagataaaaaataaaaaagtgcggtcactctggaaatcaaattttatgaccgcacttatattggtttttagcttaataaataacta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaatgtgttctccaataaacattaacatactcgaaatttccatgcgatttcggtaactgctatgacaaaaaaaatccttgaatgttcggtgggttgtacggcttttcagataaaaaataaaaaagtgcggtcactctggaaatcaaattttatgaccgcacttatattggtttttagcttaataaataacta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01869:AA01871|AA01869:AA01871:periplasmic serine protease do; hhoA-like precursor; heat shock protein:conserved hypothetical protein:->->:1258170..1258362 193 taaatgtgttctccaataaacattaacatactcgaaatttccatgcgatttcggtaactgctatgacaaaaaaaatccttgaatgttcggtgggttgtacggcttttcagataaaaaataaaaaagtgcggtcactctggaaatcaaattttatgaccgcacttatattggtttttagcttaataaataacta Intra-Species Hit: Count: 1 Min: 1 Max: 193 Len: 193 Subject: aact_AA01869_AA01871|periplasmic serine protease do; hhoA-like precursor; heat shock protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1258170,1258362]|193 HSP 1 e-value: 7.0E-43 bit: 174.0 Len: 112 Query Start:1 Query End:112 Subject Strand: POSITIVE Subject Start: 1 Subject End: 112 taaatgtgttctccaataaacattaacatactcgaaatttccatgcgatttcggtaactgctatgacnnnnnnnntccttgaatgttcggtgggttgtacggcttttcagat................................................................................. taaatgtgttctccaataaacattaacatactcgaaatttccatgcgatttcggtaactgctatgacaaaaaaaatccttgaatgttcggtgggttgtacggcttttcagat................................................................................. HSP 2 e-value: 6.0E-31 bit: 135.0 Len: 68 Query Start:126 Query End:193 Subject Strand: POSITIVE Subject Start: 126 Subject End: 193 .............................................................................................................................gtgcggtcactctggaaatcaaattttatgaccgcacttatattggtttttagcttaataaataacta .............................................................................................................................gtgcggtcactctggaaatcaaattttatgaccgcacttatattggtttttagcttaataaataacta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUGUGUUCUCCAAUAAACAUUAACAUACUCGAAAUUUCCAUGCGAUUUCGGUAACUGCUAUGACAAAAAAAAUCCUUGAAUGUUCGGUGGGUUGUACGGCUUUUCAGAUAAAAAAUAAAAAAGUGCGGUCACUCUGGAAAUCAAAUUUUAUGACCGCACUUAUAUUGGUUUUUAGCUUAAUAAAUAACUA ..(((((............)))))....(((.(((((((.......))))))))))...((((.(((......(((((((((....)))).)))))...........................(((((((((((...(((((......))))))))))))))))......)))..)))).............. (-37.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUUAUUUAUUAAGCUAAAAACCAAUAUAAGUGCGGUCAUAAAAUUUGAUUUCCAGAGUGACCGCACUUUUUUAUUUUUUAUCUGAAAAGCCGUACAACCCACCGAACAUUCAAGGAUUUUUUUUGUCAUAGCAGUUACCGAAAUCGCAUGGAAAUUUCGAGUAUGUUAAUGUUUAUUGGAGAACACAUUUA ..(((........((.((((((..((...((((((((((((....((((.....))))))))))))))))..))..)))))).))...............(((..(((((......(((.......))).(((((..(((((((((..(...)..)))))).)))))))).)))))..)))..)))....... (-37.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 414 164 122 193 151 aact:AA02902|5end_aerobic_respiration_control_protein_2031171..2031401_POSITIVE 383 164 122 200 158 aact:AA01830|5end_2,3-cyclic-nucleotide_2-phosphodiesterase_1231556..1231786_POSITIVE 383 164 122 47 5 aact:AA01829|5end_hypothetical_protein_1231403..1231633_POSITIVE 375 170 122 81 32 aact:AA02002|5end_hypothetical_protein_1360196..1360426_POSITIVE 375 164 123 176 135 aact:AA00654|5end_conserved_hypothetical_protein_447772..448002_POSITIVE 375 164 123 86 45 aact:AA00653|5end_hypothetical_protein_447682..447912_POSITIVE 373 164 121 170 124 aact:AA01117|5end_signal_recognition_particle_protein_54_(GTP-binding_export_factor)_767919..768149_POSITIVE 369 164 122 158 113 aact:AA00766|5end_heat_shock_protein_B25.3_(HSP-70_cofactor)_527384..527614_POSITIVE 366 164 123 204 165 aact:AA02754|5end_conserved_hypothetical_protein_1928949..1929179_POSITIVE 364 164 123 155 115 aact:AA02993|5end_6-phosphofructokinase_2091766..2091996_POSITIVE 345 164 122 219 174 aact:AA02778|5end_hypothetical_protein_1942585..1942815_POSITIVE 344 164 122 168 123 aact:AA00721|5end_probable_beta-N-acetylhexosaminidase_(lacto-N-biosidase);_dispersin_B_494739..494969_POSITIVE 344 164 122 56 11 aact:AA00719|5end_hypothetical_protein_494627..494857_POSITIVE 342 164 121 157 111 aact:AA02700|5end_succinyl-CoA_synthase,_alpha_subunit_1883256..1883486_POSITIVE 337 164 121 148 107 aact:AA02010|5end_peptide_methionine_sulfoxide_reductase;_cytochrome_C-TYPE_biogenesis_protein;_transcriptional_regulator_1367180..1367410_POSITIVE 337 164 122 164 123 aact:AA00899|5end_possible_quaternary_ammonium_compound_resistance_protein_623214..623444_POSITIVE 333 170 121 168 119 aact:AA02128|5end_DNA_topoisomerase_I_1451395..1451625_POSITIVE 327 164 122 118 78 aact:AA02362|5end_conserved_hypothetical_protein_1619905..1620135_POSITIVE 324 164 122 149 109 aact:AA02084|5end_conserved_hypothetical_protein_1417843..1418073_POSITIVE 323 164 121 184 142 aact:AA02722|5end_cytidine_deaminase_1905379..1905609_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 414 164 122 80 38 aact:AA02899|3end_oligopeptide_ABC_transporter,_ATP-binding_protein_2031138..2031288_POSITIVE 384 164 122 77 35 aact:AA00218|3end_argininosuccinate_lyase_152211..152361_POSITIVE 366 164 123 43 4 aact:AA02751|3end_L-lactate_permease_1928868..1929018_POSITIVE 364 164 123 49 9 aact:AA02992|3end_RNA_methyltransferase_2091740..2091890_POSITIVE 347 170 121 86 36 aact:AA02781|3end_protein-export_membrane_protein_1945981..1946131_POSITIVE 345 164 122 81 36 aact:AA02777|3end_tRNA-guanine_transglycosylase_1942527..1942677_POSITIVE 344 164 122 72 27 aact:AA00718|3end_ABC-related_ATP-binding_protein;_MRP_protein_494723..494873_POSITIVE 337 164 122 76 35 aact:AA00896|3end_prolyl-tRNA_synthetase_(proline-tRNA_ligase)_623206..623356_POSITIVE 333 170 121 77 28 aact:AA02126|3end_conserved_hypothetical_protein_1451384..1451534_POSITIVE 327 164 122 80 40 aact:AA02360|3end_threonine_synthase_1619947..1620097_POSITIVE 327 170 122 93 45 aact:AA02280|3end_conserved_hypothetical_protein_1552767..1552917_POSITIVE 324 164 122 74 34 aact:AA02082|3end_glutaminyl-tRNA_synthetase_1417848..1417998_POSITIVE 323 164 121 59 17 aact:AA02721|3end_spermidine/putrescine_ABC_transporter,_periplasmic-binding_protein_1905334..1905484_POSITIVE 321 161 121 107 69 aact:AA01270|3end_hydrolase_(possible_2-haloalkanoic_acid_dehalogenase_I)_863897..864047_POSITIVE 319 164 123 83 42 aact:AA02892|3end_oligopeptide_ABC_transporter,_periplasmic-binding_protein_2027177..2027327_POSITIVE 316 161 122 145 107 aact:AA00623|3end_hypothetical_protein_430632..430782_POSITIVE 316 164 121 123 81 aact:AA00495|3end_hypothetical_protein_345139..345289_POSITIVE 314 164 122 56 16 aact:AA00010|3end_transposase_orfB_fragment_6297..6447_POSITIVE 313 169 121 92 42 aact:AA01557|3end_recombinase_A_1045965..1046115_POSITIVE 313 164 122 80 40 aact:AA00191|3end_conserved_hypothetical_protein_(possible_acetyltransferase,_GNAT_family)_135545..135695_POSITIVE