Origin IGS: taatggtggattcctttgaagggcatgtgaagtactaagcccttatagggtagcctaaaaaccgcacgttttacgtgcggattggtatttttattgcagatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatctgcaataaaaataccaatccgcacgtaaaacgtgcggtttttaggctaccctataagggcttagtacttcacatgcccttcaaaggaatccaccatta Mask Tandem Repeat Region ================================================ taatggtggattcctttgaagggcatgtgaagtactaagcccttatagggtagcctaaaaaccgcacgttttacgtgcggattggtatttttattgcagatta Find is-nt database================================================ Query_seq: AA01868:AA01869|AA01868:AA01869:biotin synthetase:periplasmic serine protease do; hhoA-like precursor; heat shock protein:->->:1256687..1256789 103 taatggtggattcctttgaagggcatgtgaagtactaagcccttatagggtagcctaaaaaccgcacgttttacgtgcggattggtatttttattgcagatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01868:AA01869|AA01868:AA01869:biotin synthetase:periplasmic serine protease do; hhoA-like precursor; heat shock protein:->->:1256687..1256789 103 taatggtggattcctttgaagggcatgtgaagtactaagcccttatagggtagcctaaaaaccgcacgttttacgtgcggattggtatttttattgcagatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01868:AA01869|AA01868:AA01869:biotin synthetase:periplasmic serine protease do; hhoA-like precursor; heat shock protein:->->:1256687..1256789 103 taatggtggattcctttgaagggcatgtgaagtactaagcccttatagggtagcctaaaaaccgcacgttttacgtgcggattggtatttttattgcagatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatggtggattcctttgaagggcatgtgaagtactaagcccttatagggtagcctaaaaaccgcacgttttacgtgcggattggtatttttattgcagatta Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: - Start: 3 End: 101 .. H H I G K F P M H L V L G K Y P L R F V A R K V H P N T N K N C M .. taatggtggattcctttgaagggcatgtgaagtactaagcccttatagggtagcctaaaaaccgcacgttttacgtgcggattggtatttttattgcagatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatggtggattcctttgaagggcatgtgaagtactaagcccttatagggtagcctaaaaaccgcacgttttacgtgcggattggtatttttattgcagatta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 79 HP_score: -5.2 Tail_Score: -5.08088 Start: 56 End: 87 Full_Region: CCTTATAGGGTAGCC TAAAAACCGCACGT TTT ACGTGCGGATTGGTA TTTTTATTGCAGATT .......................................................TAAAAACCGCACGTTTTACGTGCGGATTGGTA................ TransTerm Strand: + Conf: 83 HP_score: -11.1 Tail_Score: -3.66615 Start: 61 End: 81 Full_Region: TAGGGTAGCCTAAAA ACCGCACGT TTT ACGTGCGGA TTGGTATTTTTATTG ............................................................ACCGCACGTTTTACGTGCGGA...................... TransTerm Strand: + Conf: 89 HP_score: -14.6 Tail_Score: -2.78342 Start: 62 End: 80 Full_Region: AGGGTAGCCTAAAAA CCGCACGT TTT ACGTGCGG ATTGGTATTTTTATT .............................................................CCGCACGTTTTACGTGCGG....................... Find igs database================================================ Query_seq: AA01868:AA01869|AA01868:AA01869:biotin synthetase:periplasmic serine protease do; hhoA-like precursor; heat shock protein:->->:1256687..1256789 103 taatggtggattcctttgaagggcatgtgaagtactaagcccttatagggtagcctaaaaaccgcacgttttacgtgcggattggtatttttattgcagatta Intra-Species Hit: Count: 1 Min: 1 Max: 103 Len: 103 Subject: aact_AA01868_AA01869|biotin synthetase:periplasmic serine protease do; hhoA-like precursor; heat shock protein|POSITIVE:POSITIVE|[1256687,1256789]|103 HSP 1 e-value: 4.0E-52 bit: 204.0 Len: 103 Query Start:1 Query End:103 Subject Strand: POSITIVE Subject Start: 1 Subject End: 103 taatggtggattcctttgaagggcatgtgaagtactaagcccttatagggtagcctaaaaaccgcacgttttacgtgcggattggtatttttattgcagatta taatggtggattcctttgaagggcatgtgaagtactaagcccttatagggtagcctaaaaaccgcacgttttacgtgcggattggtatttttattgcagatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUGGUGGAUUCCUUUGAAGGGCAUGUGAAGUACUAAGCCCUUAUAGGGUAGCCUAAAAACCGCACGUUUUACGUGCGGAUUGGUAUUUUUAUUGCAGAUUA ....(((....((((...((((((..(((...)))...))))))..))))..)))......(((((((.....)))))))....(((.......)))...... (-31.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCUGCAAUAAAAAUACCAAUCCGCACGUAAAACGUGCGGUUUUUAGGCUACCCUAUAAGGGCUUAGUACUUCACAUGCCCUUCAAAGGAAUCCACCAUUA ......((..((((((.......(((((((.....))))))))))))).))...(((..((((((...((.....))..))))))...)))............ (-25.41) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 811 90 41 210 161 aact:AA00357|5end_transposase_(probable_IS200-like_)_243334..243564_POSITIVE 316 80 31 134 87 aact:AA00401|5end_FKBP-type_peptidyl-prolyl_cis-trans_isomerase_slyD_(PPiase)_268361..268591_POSITIVE 315 70 26 53 7 aact:AA01386|5end_30S_ribosomal_protein_S1_935974..936204_POSITIVE 302 97 54 160 103 aact:AA02252|5end_conserved_hypothetical_protein_1533840..1534070_POSITIVE 301 78 32 220 170 aact:AA01287|5end_transposase_871583..871813_POSITIVE 299 83 41 231 188 aact:AA02577|5end_nuclease_1795267..1795497_POSITIVE 296 97 61 163 120 aact:AA00272|5end_3-oxoacyl-[acyl-carrier-protein]_synthase_III_190406..190636_POSITIVE 295 55 14 64 21 aact:AA02777|5end_tRNA-guanine_transglycosylase_1941299..1941529_POSITIVE 295 80 47 104 75 aact:AA01227|5end_hypothetical_protein_838619..838849_POSITIVE 290 87 41 128 86 aact:AA01724|5end_valyl-tRNA_synthetase_1164446..1164676_POSITIVE 289 81 41 122 82 aact:AA01966|5end_2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_pyrophosphokinase_1330937..1331167_POSITIVE 289 85 58 213 184 aact:AA00564|5end_elongation_factor_G_387755..387985_POSITIVE 289 79 49 40 7 aact:AA00212|5end_ribokinase_146056..146286_POSITIVE 288 100 61 103 66 aact:AA02965|5end_hypothetical_protein_2074069..2074299_POSITIVE 286 80 39 200 144 aact:AA00544|5end_glutathionylspermidine_synthetase;amidase_372448..372678_POSITIVE 284 85 60 155 126 aact:AA00712|5end_chaperone_protein_hscB,_heat_shock_cognate_488228..488458_POSITIVE 282 69 22 231 180 aact:AA01593|5end_glycosyltransferase;_rfbJ_protein_1073131..1073361_POSITIVE 280 80 34 143 92 aact:AA01472|5end_hypothetical_protein_988896..989126_POSITIVE 279 80 39 56 15 aact:AA01626|5end_N_utilization_substance_protein_B_1100444..1100674_POSITIVE 276 78 39 63 2 aact:AA01805|5end_electron_transport_complex_protein_1216115..1216345_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 811 90 41 78 29 aact:AA00356|3end_DNA-directed_RNA_polymerase,_beta'_chain_243282..243432_POSITIVE 328 79 31 67 8 aact:AA00211|3end_hypothetical_protein_146163..146313_POSITIVE 316 80 31 56 9 aact:AA00399|3end_conserved_hypothetical_protein_268363..268513_POSITIVE 302 97 54 83 26 aact:AA02251|3end_biotin_sulfoxide_reductase;_Trimethylamine-N-oxide_reductase_precursor_1533843..1533993_POSITIVE 296 97 61 146 103 aact:AA00271|3end_hypothetical_protein_190469..190619_POSITIVE 295 80 32 61 10 aact:AA00371|3end_transcriptional_regulator_250154..250304_POSITIVE 289 81 41 126 86 aact:AA01965|3end_poly(A)_polymerase_1331021..1331171_POSITIVE 289 85 58 146 117 aact:AA00563|3end_elongation_factor_Tu_387768..387918_POSITIVE 288 100 61 82 45 aact:AA02964|3end_opacity_associated_protein_A_2074128..2074278_POSITIVE 287 91 62 44 11 aact:AA01868|3end_biotin_synthetase_1256626..1256776_POSITIVE 286 80 39 79 23 aact:AA00543|3end_ADP-heptose--LPS_heptosyltransferase_II_372407..372557_POSITIVE 285 80 36 44 1 aact:AA02324|3end_ABC_transporter_ATP_binding_protein_1587392..1587542_POSITIVE 284 85 60 140 111 aact:AA00711|3end_conserved_hypothetical_protein_488293..488443_POSITIVE 280 88 49 149 108 aact:AA00480|3end_mannose-specific_phosphotransferase_system_IID_335199..335349_POSITIVE 279 80 39 46 5 aact:AA01625|3end_riboflavin_synthase,_beta_chain_1100514..1100664_POSITIVE 274 80 34 79 24 aact:AA01800|3end_hypothetical_protein_1213458..1213608_POSITIVE 274 87 61 109 80 aact:AA01089|3end_hypothetical_protein_753597..753747_POSITIVE 273 80 39 54 8 aact:AA00782|3end_conserved_hypothetical_protein_539120..539270_POSITIVE 269 80 31 75 23 aact:AA02619|3end_GTP_cyclohydrolase_II_1826193..1826343_POSITIVE 269 96 62 148 117 aact:AA02425|3end_glutamate-1-semialdehyde_aminotransferase_1671661..1671811_POSITIVE