Origin IGS: ataactcctattcatgataaaaaatccccgctattctaatccatagaagggattgctttaaagttatttcgttaataaatccaacgcttcttgatatttctgcaattactttggcgacactttgtcggaatagatttttttaagactgagttgtgtcattttattaccttttatccgagcaaaaatca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgatttttgctcggataaaaggtaataaaatgacacaactcagtcttaaaaaaatctattccgacaaagtgtcgccaaagtaattgcagaaatatcaagaagcgttggatttattaacgaaataactttaaagcaatcccttctatggattagaatagcggggattttttatcatgaataggagttat Mask Tandem Repeat Region ================================================ ataactcctattcatgataaaaaatccccgctattctaatccatagaagggattgctttaaagttatttcgttaataaatccaacgcttcttgatatttctgcaattactttggcgacactttgtcggaatagatttttttaagactgagttgtgtcattttattaccttttatccgagcaaaaatca Find is-nt database================================================ Query_seq: AA01850:AA01851|AA01850:AA01851:conserved hypothetical protein; possible enzyme of sugar metabolism:hypothetical protein:->->:1244675..1244862 188 ataactcctattcatgataaaaaatccccgctattctaatccatagaagggattgctttaaagttatttcgttaataaatccaacgcttcttgatatttctgcaattactttggcgacactttgtcggaatagatttttttaagactgagttgtgtcattttattaccttttatccgagcaaaaatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01850:AA01851|AA01850:AA01851:conserved hypothetical protein; possible enzyme of sugar metabolism:hypothetical protein:->->:1244675..1244862 188 ataactcctattcatgataaaaaatccccgctattctaatccatagaagggattgctttaaagttatttcgttaataaatccaacgcttcttgatatttctgcaattactttggcgacactttgtcggaatagatttttttaagactgagttgtgtcattttattaccttttatccgagcaaaaatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01850:AA01851|AA01850:AA01851:conserved hypothetical protein; possible enzyme of sugar metabolism:hypothetical protein:->->:1244675..1244862 188 ataactcctattcatgataaaaaatccccgctattctaatccatagaagggattgctttaaagttatttcgttaataaatccaacgcttcttgatatttctgcaattactttggcgacactttgtcggaatagatttttttaagactgagttgtgtcattttattaccttttatccgagcaaaaatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ataactcctattcatgataaaaaatccccgctattctaatccatagaagggattgctttaaagttatttcgttaataaatccaacgcttcttgatatttctgcaattactttggcgacactttgtcggaatagatttttttaagactgagttgtgtcattttattaccttttatccgagcaaaaatca Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: + Start: 24 End: 140 ....................... M P A I L I H R R D C F K V I S L I N P T L L D I S A I T L A T L C R N R F F ................................................ Protein_Len: 36 Strand: + Start: 79 End: 186 .............................................................................. M Q R F L I F L Q L L W R H F V G I D F F K T E L C H F I T F Y P S K N .. Protein_Len: 32 Strand: - Start: 11 End: 106 .......... E H Y F I G A I R I W L L S Q K L T I E N I F G V S R S I E A M .................................................................................. ataactcctattcatgataaaaaatccccgctattctaatccatagaagggattgctttaaagttatttcgttaataaatccaacgcttcttgatatttctgcaattactttggcgacactttgtcggaatagatttttttaagactgagttgtgtcattttattaccttttatccgagcaaaaatca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 33 End: 62 ................................TTTAAAGCAATCCCTTCTATGGATTAGAAT.............................................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ataactcctattcatgataaaaaatccccgctattctaatccatagaagggattgctttaaagttatttcgttaataaatccaacgcttcttgatatttctgcaattactttggcgacactttgtcggaatagatttttttaagactgagttgtgtcattttattaccttttatccgagcaaaaatca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01850:AA01851|AA01850:AA01851:conserved hypothetical protein; possible enzyme of sugar metabolism:hypothetical protein:->->:1244675..1244862 188 ataactcctattcatgataaaaaatccccgctattctaatccatagaagggattgctttaaagttatttcgttaataaatccaacgcttcttgatatttctgcaattactttggcgacactttgtcggaatagatttttttaagactgagttgtgtcattttattaccttttatccgagcaaaaatca Intra-Species Hit: Count: 1 Min: 1 Max: 188 Len: 188 Subject: aact_AA01850_AA01851|conserved hypothetical protein; possible enzyme of sugar metabolism:hypothetical protein|POSITIVE:POSITIVE|[1244675,1244862]|188 HSP 1 e-value: 5.0E-90 bit: 331.0 Len: 188 Query Start:1 Query End:188 Subject Strand: POSITIVE Subject Start: 1 Subject End: 188 ataactcctattcatgataaaaaatccccgctattctaatccatagaagggattgctttaaagttatttcgttaataaatccaacgcttcttgatatttctgcaattactttggcgacactttgtcggaatagannnnnnnaagactgagttgtgtcattttattaccttttatccgagcaaaaatca ataactcctattcatgataaaaaatccccgctattctaatccatagaagggattgctttaaagttatttcgttaataaatccaacgcttcttgatatttctgcaattactttggcgacactttgtcggaatagatttttttaagactgagttgtgtcattttattaccttttatccgagcaaaaatca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAACUCCUAUUCAUGAUAAAAAAUCCCCGCUAUUCUAAUCCAUAGAAGGGAUUGCUUUAAAGUUAUUUCGUUAAUAAAUCCAACGCUUCUUGAUAUUUCUGCAAUUACUUUGGCGACACUUUGUCGGAAUAGAUUUUUUUAAGACUGAGUUGUGUCAUUUUAUUACCUUUUAUCCGAGCAAAAAUCA ..........(((..(((((((((((((..((((........))))..))))))..................(((((((.(((((.(.((((((....(((((((.....))).((((.....))))...)))).....))))))..).)))).)....)))))))..))))))).)))......... (-29.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUUUUUGCUCGGAUAAAAGGUAAUAAAAUGACACAACUCAGUCUUAAAAAAAUCUAUUCCGACAAAGUGUCGCCAAAGUAAUUGCAGAAAUAUCAAGAAGCGUUGGAUUUAUUAACGAAAUAACUUUAAAGCAAUCCCUUCUAUGGAUUAGAAUAGCGGGGAUUUUUUAUCAUGAAUAGGAGUUAU (((((((((.((((((((((.(((((.....(((........)))................((((.....))))........)))))..............((.(((((.(((((.....))))).))))).)).((((((.((((........)))).)))))).))))))).))).))))))))). (-36.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 326 127 82 213 174 aact:AA02382|5end_glucuronate/uronate_isomerase_1629842..1630072_POSITIVE 314 118 80 64 21 aact:AA01679|5end_aspartate-semialdehyde_dehydrogenase;_PTS_system_enzyme_II_A_component_1134356..1134586_POSITIVE 302 103 62 195 154 aact:AA01400|5end_phosphopantetheine_adenylyltransferase_945679..945909_POSITIVE 295 129 84 185 132 aact:AA01338|5end_hypothetical_protein_904967..905197_POSITIVE 282 129 85 55 19 aact:AA00908|5end_probable_sigma-E_factor_regulatory_protein_626076..626306_POSITIVE 274 170 126 105 65 aact:AA00774|5end_intracellular_septation_protein_532468..532698_POSITIVE 271 119 81 213 180 aact:AA02509|5end_chorismate_synthase_1740952..1741182_POSITIVE 271 129 85 153 114 aact:AA02199|5end_hypothetical_protein_1497627..1497857_POSITIVE 265 37 1 112 72 aact:AA02802|5end_probable_myo-inositol_2-dehydrogenase_1968999..1969229_POSITIVE 265 60 22 203 168 aact:AA00782|5end_conserved_hypothetical_protein_538723..538953_POSITIVE 261 129 81 230 177 aact:AA01685|5end_molybdenum_ABC_transporter,_ATP-binding_protein_1136979..1137209_POSITIVE 260 128 81 158 115 aact:AA01553|5end_conserved_hypothetical_protein_1039220..1039450_POSITIVE 260 130 81 157 115 aact:AA00909.1|5end_hypothetical_protein_625970..626200_POSITIVE 259 56 26 165 135 aact:AA01160|5end_ADP_compounds_hydrolase_793565..793795_POSITIVE 257 130 110 29 8 aact:AA02369|5end_conserved_hypothetical_protein_1624248..1624478_POSITIVE 256 128 85 182 138 aact:AA00805|5end_fumarate_reductase_iron-sulfur_protein_551583..551813_POSITIVE 251 130 90 106 51 aact:AA00321|5end_hypothetical_protein_219054..219284_POSITIVE 250 59 11 158 104 aact:AA00511|5end_nicotinamide_mononucleotide_transporter_353884..354114_POSITIVE 248 129 83 91 41 aact:AA00890|5end_conserved_hypothetical_protein_617075..617305_POSITIVE 246 127 82 222 155 aact:AA01274|5end_hypothetical_protein_864519..864749_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 302 103 62 127 86 aact:AA01398|3end_KDO_kinase_945691..945841_POSITIVE 299 130 85 99 54 aact:AA01573|3end_peptide_transport_periplasmic_protein_1061454..1061604_POSITIVE 282 129 85 42 6 aact:AA00906|3end_sigma-E_factor_regulatory_protein_626143..626293_POSITIVE 274 170 126 96 56 aact:AA00773|3end_conserved_hypothetical_protein_532539..532689_POSITIVE 271 129 85 138 99 aact:AA02196|3end_oxaloacetate_decarboxylase_beta_chain_1497692..1497842_POSITIVE 265 37 1 106 66 aact:AA02801|3end_myo-inositol_catabolism_protein_1969073..1969223_POSITIVE 250 59 11 77 23 aact:AA00508|3end_SAM-dependent_methyltransferase_353883..354033_POSITIVE 240 52 11 74 33 aact:AA01724|3end_valyl-tRNA_synthetase_1167222..1167372_POSITIVE 240 136 91 137 89 aact:AA00296|3end_conserved_hypothetical_protein_(possible_hemolysin_/_transport_protein)_203714..203864_POSITIVE 238 159 112 72 12 aact:AA00699|3end_iron(III)_ABC_transporter,_permease_protein_480396..480546_POSITIVE 234 59 11 134 73 aact:AA01137|3end_anaerobic_dimethyl_sulfoxide_reductase,_chain_A_781057..781207_POSITIVE 234 160 115 112 71 aact:AA00241|3end_formate_acetyltransferase_activating_enzyme_(anaerobic_ribonucleoside-triphosphate_reductase_activating_protein)_172090..172240_POSITIVE 232 160 112 131 85 aact:AA02139|3end_possible_membrane_protein_1462218..1462368_POSITIVE 226 130 83 58 15 aact:AA02235|3end_stringent_starvation_protein_B_1521984..1522134_POSITIVE 226 155 112 135 96 aact:AA01900|3end_hypothetical_protein_1282582..1282732_POSITIVE 226 70 24 70 18 aact:AA01890|3end_cell-cycle_protein_1275967..1276117_POSITIVE 226 129 85 126 86 aact:AA01750|3end_MTA/SAH_nucleosidase_[Includes:_5'-methylthioadenosine_nucleosidase;_S-adenosylhomocysteine_nucleosidase]_1184519..1184669_POSITIVE 226 135 91 77 34 aact:AA01644|3end_ferric_transport_system_permease_protein_1114160..1114310_POSITIVE 225 155 112 134 78 aact:AA01071|3end_RNA_methyltransferase_743001..743151_POSITIVE 224 130 84 133 93 aact:AA00221|3end_NADP-specific_glutamate_dehydrogenase_153865..154015_POSITIVE