Origin IGS: aagttactccaaatcaggtaataagaaagggcgctatgatattgcaaatggcaggcgattgcaaaatatatgcagaggagggggcttggttaattttcctcaagttccgaaagataaaacagccccaatgaggctgaattatgttcaataacttaaggcaatttgttgttaccttggcgaaaaaacaactta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taagttgttttttcgccaaggtaacaacaaattgccttaagttattgaacataattcagcctcattggggctgttttatctttcggaacttgaggaaaattaaccaagccccctcctctgcatatattttgcaatcgcctgccatttgcaatatcatagcgccctttcttattacctgatttggagtaactt Mask Tandem Repeat Region ================================================ aagttactccaaatcaggtaataagaaagggcgctatgatattgcaaatggcaggcgattgcaaaatatatgcagaggagggggcttggttaattttcctcaagttccgaaagataaaacagccccaatgaggctgaattatgttcaataacttaaggcaatttgttgttaccttggcgaaaaaacaactta Find is-nt database================================================ Query_seq: AA01846:AA01848|AA01846:AA01848:conserved hypothetical protein:argininosuccinate synthetase:->->:1242226..1242417 192 aagttactccaaatcaggtaataagaaagggcgctatgatattgcaaatggcaggcgattgcaaaatatatgcagaggagggggcttggttaattttcctcaagttccgaaagataaaacagccccaatgaggctgaattatgttcaataacttaaggcaatttgttgttaccttggcgaaaaaacaactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01846:AA01848|AA01846:AA01848:conserved hypothetical protein:argininosuccinate synthetase:->->:1242226..1242417 192 aagttactccaaatcaggtaataagaaagggcgctatgatattgcaaatggcaggcgattgcaaaatatatgcagaggagggggcttggttaattttcctcaagttccgaaagataaaacagccccaatgaggctgaattatgttcaataacttaaggcaatttgttgttaccttggcgaaaaaacaactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01846:AA01848|AA01846:AA01848:conserved hypothetical protein:argininosuccinate synthetase:->->:1242226..1242417 192 aagttactccaaatcaggtaataagaaagggcgctatgatattgcaaatggcaggcgattgcaaaatatatgcagaggagggggcttggttaattttcctcaagttccgaaagataaaacagccccaatgaggctgaattatgttcaataacttaaggcaatttgttgttaccttggcgaaaaaacaactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aagttactccaaatcaggtaataagaaagggcgctatgatattgcaaatggcaggcgattgcaaaatatatgcagaggagggggcttggttaattttcctcaagttccgaaagataaaacagccccaatgaggctgaattatgttcaataacttaaggcaatttgttgttaccttggcgaaaaaacaactta Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 21 End: 128 .................... M R K G A M I L Q M A G D C K I Y A E E G A W L I F L K F R K I K Q P Q ................................................................ Protein_Len: 36 Strand: + Start: 41 End: 148 ........................................ M A N G R R L Q N I C R G G G L V N F P Q V P K D K T A P M R L N Y V Q ............................................ Protein_Len: 44 Strand: - Start: 17 End: 148 ................ T I L F P A I I N C I A P S Q L I Y A S S P A Q N I K R L N R F I F C G W H P Q I I N M ............................................ aagttactccaaatcaggtaataagaaagggcgctatgatattgcaaatggcaggcgattgcaaaatatatgcagaggagggggcttggttaattttcctcaagttccgaaagataaaacagccccaatgaggctgaattatgttcaataacttaaggcaatttgttgttaccttggcgaaaaaacaactta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 84 Start: 36 End: 65 ...................................TTTGCAATCGCCTGCCATTTGCAATATCAT............................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aagttactccaaatcaggtaataagaaagggcgctatgatattgcaaatggcaggcgattgcaaaatatatgcagaggagggggcttggttaattttcctcaagttccgaaagataaaacagccccaatgaggctgaattatgttcaataacttaaggcaatttgttgttaccttggcgaaaaaacaactta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 91 HP_score: -10.1 Tail_Score: -4.78848 Start: 120 End: 136 Full_Region: ttattgaacataatt cagcctc att ggggctg ttttatctttcggaa .......................................................................................................................cagcctcattggggctg........................................................ Find igs database================================================ Query_seq: AA01846:AA01848|AA01846:AA01848:conserved hypothetical protein:argininosuccinate synthetase:->->:1242226..1242417 192 aagttactccaaatcaggtaataagaaagggcgctatgatattgcaaatggcaggcgattgcaaaatatatgcagaggagggggcttggttaattttcctcaagttccgaaagataaaacagccccaatgaggctgaattatgttcaataacttaaggcaatttgttgttaccttggcgaaaaaacaactta Intra-Species Hit: Count: 1 Min: 1 Max: 192 Len: 192 Subject: aact_AA01846_AA01848|conserved hypothetical protein:argininosuccinate synthetase|POSITIVE:POSITIVE|[1242226,1242417]|192 HSP 1 e-value: 1.0E-105 bit: 381.0 Len: 192 Query Start:1 Query End:192 Subject Strand: POSITIVE Subject Start: 1 Subject End: 192 aagttactccaaatcaggtaataagaaagggcgctatgatattgcaaatggcaggcgattgcaaaatatatgcagaggagggggcttggttaattttcctcaagttccgaaagataaaacagccccaatgaggctgaattatgttcaataacttaaggcaatttgttgttaccttggcgaaaaaacaactta aagttactccaaatcaggtaataagaaagggcgctatgatattgcaaatggcaggcgattgcaaaatatatgcagaggagggggcttggttaattttcctcaagttccgaaagataaaacagccccaatgaggctgaattatgttcaataacttaaggcaatttgttgttaccttggcgaaaaaacaactta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGUUACUCCAAAUCAGGUAAUAAGAAAGGGCGCUAUGAUAUUGCAAAUGGCAGGCGAUUGCAAAAUAUAUGCAGAGGAGGGGGCUUGGUUAAUUUUCCUCAAGUUCCGAAAGAUAAAACAGCCCCAAUGAGGCUGAAUUAUGUUCAAUAACUUAAGGCAAUUUGUUGUUACCUUGGCGAAAAAACAACUUA ......(.(((....(((((((((.(((..((.((..(.(((((...(((.(((.(..(((((.......))))).....((((((...(((.(((((..........))))).)))...)))))).....).)))...)))...))))).)...))))..))).)))))))))))).)............. (-44.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGUUGUUUUUUCGCCAAGGUAACAACAAAUUGCCUUAAGUUAUUGAACAUAAUUCAGCCUCAUUGGGGCUGUUUUAUCUUUCGGAACUUGAGGAAAAUUAACCAAGCCCCCUCCUCUGCAUAUAUUUUGCAAUCGCCUGCCAUUUGCAAUAUCAUAGCGCCCUUUCUUAUUACCUGAUUUGGAGUAACUU .((((((((((.......((((((..........(((((((((.(((((.((((..((((((.....))))))..))))..))))))))))))))........................((.(((((.((((((...........)))))).)).)))))...........)))))).....)))))))))) (-43.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 378 140 94 158 108 aact:AA00982|5end_aldo-keto_reductase_686526..686756_POSITIVE 334 141 105 186 146 aact:AA01354|5end_conserved_hypothetical_protein_916489..916719_POSITIVE 332 169 123 136 83 aact:AA01773|5end_hypothetical_protein_1194877..1195107_POSITIVE 330 90 43 216 174 aact:AA00129|5end_hypothetical_protein_94122..94352_POSITIVE 329 89 42 194 148 aact:AA00514|5end_hypothetical_protein_355609..355839_POSITIVE 328 90 44 77 24 aact:AA00762|5end_hemoglobin_binding_protein_A_524199..524429_POSITIVE 321 79 31 74 37 aact:AA02101|5end_penicillin-binding_protein_7_1430830..1431060_POSITIVE 319 78 31 197 149 aact:AA00506|5end_ATP-dependent_RNA_helicase_protein_351681..351911_POSITIVE 319 78 31 77 29 aact:AA00505|5end_hypothetical_protein_351561..351791_POSITIVE 318 90 44 87 25 aact:AA01050|5end_iron(III)_ABC_transporter,_permease_protein_730501..730731_POSITIVE 314 100 54 107 50 aact:AA01182|5end_3-oxoacyl-(acyl-carrier-protein)_synthase_809210..809440_POSITIVE 312 62 27 222 186 aact:AA00335|5end_PTS_fructose-specific_IIBC_component_226489..226719_POSITIVE 308 119 77 185 139 aact:AA00695|5end_uridine_kinase_475033..475263_POSITIVE 307 57 12 193 148 aact:AA01807|5end_electron_transport_complex_protein_1216696..1216926_POSITIVE 301 57 16 196 146 aact:AA00298|5end_hypothetical_protein_203624..203854_POSITIVE 299 78 32 48 1 aact:AA02883|5end_conserved_hypothetical_protein_2021418..2021648_POSITIVE 298 100 53 194 145 aact:AA00766|5end_heat_shock_protein_B25.3_(HSP-70_cofactor)_527384..527614_POSITIVE 296 89 48 204 169 aact:AA01841|5end_conserved_hypothetical_protein_1237514..1237744_POSITIVE 295 90 43 67 22 aact:AA02895|5end_oligopeptide_transport_system_permease_protein,_C-terminal_2027484..2027714_POSITIVE 295 92 51 79 40 aact:AA01197|5end_conserved_hypothetical_protein_820863..821093_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 332 169 123 121 68 aact:AA01772|3end_shikimate_5-dehydrogenase_protein_1194942..1195092_POSITIVE 314 100 54 111 54 aact:AA01181|3end_conserved_hypothetical_protein_809294..809444_POSITIVE 307 61 30 132 93 aact:AA02003|3end_purine_nucleotide_synthesis_repressor_protein_1361708..1361858_POSITIVE 298 100 53 77 28 aact:AA00764|3end_hemoglobin_binding_protein_A_527347..527497_POSITIVE 295 90 43 47 2 aact:AA02893|3end_oligopeptide_transport_system_permease_protein,_N-terminal_2027544..2027694_POSITIVE 295 92 51 91 52 aact:AA01196|3end_1-acyl-sn-glycerol-3-phosphate_acetyltransferase_820955..821105_POSITIVE 291 63 21 143 102 aact:AA01039|3end_transcriptional_regulator_(possible_murein_gene_regulator)_724252..724402_POSITIVE 290 86 47 59 21 aact:AA01172|3end_conserved_hypothetical_protein_803847..803997_POSITIVE 285 89 52 94 52 aact:AA02480|3end_excinuclease_ABC_subunit_B_1718442..1718592_POSITIVE 283 80 31 100 37 aact:AA02430|3end_conserved_hypothetical_protein_1675669..1675819_POSITIVE 281 90 43 95 50 aact:AA02224|3end_ABC_transport_system_permease_protein_1513282..1513432_POSITIVE 277 89 41 65 15 aact:AA01744|3end_peptide_chain_release_factor_RF-2_1180352..1180502_POSITIVE 276 90 44 145 84 aact:AA00549|3end_ferredoxin-type_protein_376521..376671_POSITIVE 276 79 31 100 60 aact:AA00244|3end_conserved_hypothetical_protein_(possible_deoxyribonuclease,_possible_hydrolase)_172812..172962_POSITIVE 274 89 44 136 84 aact:AA02682|3end_hydrogenase_maturation_protein_1868666..1868816_POSITIVE 272 135 105 67 35 aact:AA01304|3end_penicillin-binding_protein_1A_885580..885730_POSITIVE 271 78 32 75 38 aact:AA01750|3end_MTA/SAH_nucleosidase_[Includes:_5'-methylthioadenosine_nucleosidase;_S-adenosylhomocysteine_nucleosidase]_1184519..1184669_POSITIVE 265 68 29 100 59 aact:AA00646|3end_2-dehydro-3-deoxyphosphooctonate_aldolase_442405..442555_POSITIVE 265 88 48 143 91 aact:AA00363|3end_50S_ribosomal_protein_L22_245980..246130_POSITIVE 264 80 32 69 13 aact:AA02844|3end_Holliday_junction_DNA_helicase_1997885..1998035_POSITIVE