Origin IGS: attttctcctgttgtgtgtgaaataaccgctttaagattaacctaattttgctgattaaaacaacaagagatcacaaaatgaagggtaaatttgtgacctagatcacatattttgatactttttctaatgtttgagataaataaattttattagtgataagttagagaaaagcatgcggtcgtccgtgatataaaaacacataaaaaaccgaccgcacttttgcgaagagattta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaatctcttcgcaaaagtgcggtcggttttttatgtgtttttatatcacggacgaccgcatgcttttctctaacttatcactaataaaatttatttatctcaaacattagaaaaagtatcaaaatatgtgatctaggtcacaaatttacccttcattttgtgatctcttgttgttttaatcagcaaaattaggttaatcttaaagcggttatttcacacacaacaggagaaaat Mask Tandem Repeat Region ================================================ attttctcctgttgtgtgtgaaataaccgctttaagattaacctaattttgctgattaaaacaacaagagatcacaaaatgaagggtaaatttgtgacctagatcacatattttgatactttttctaatgtttgagataaataaattttattagtgataagttagagaaaagcatgcggtcgtccgtgatataaaaacacataaaaaaccgaccgcacttttgcgaagagattta Find is-nt database================================================ Query_seq: AA01845:AA01846|AA01845:AA01846:gamma-glutamyltranspeptidase precursor:conserved hypothetical protein:->->:1241748..1241982 235 attttctcctgttgtgtgtgaaataaccgctttaagattaacctaattttgctgattaaaacaacaagagatcacaaaatgaagggtaaatttgtgacctagatcacatattttgatactttttctaatgtttgagataaataaattttattagtgataagttagagaaaagcatgcggtcgtccgtgatataaaaacacataaaaaaccgaccgcacttttgcgaagagattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01845:AA01846|AA01845:AA01846:gamma-glutamyltranspeptidase precursor:conserved hypothetical protein:->->:1241748..1241982 235 attttctcctgttgtgtgtgaaataaccgctttaagattaacctaattttgctgattaaaacaacaagagatcacaaaatgaagggtaaatttgtgacctagatcacatattttgatactttttctaatgtttgagataaataaattttattagtgataagttagagaaaagcatgcggtcgtccgtgatataaaaacacataaaaaaccgaccgcacttttgcgaagagattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01845:AA01846|AA01845:AA01846:gamma-glutamyltranspeptidase precursor:conserved hypothetical protein:->->:1241748..1241982 235 attttctcctgttgtgtgtgaaataaccgctttaagattaacctaattttgctgattaaaacaacaagagatcacaaaatgaagggtaaatttgtgacctagatcacatattttgatactttttctaatgtttgagataaataaattttattagtgataagttagagaaaagcatgcggtcgtccgtgatataaaaacacataaaaaaccgaccgcacttttgcgaagagattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 attttctcctgttgtgtgtgaaataaccgctttaagattaacctaattttgctgattaaaacaacaagagatcacaaaatgaagggtaaatttgtgacctagatcacatattttgatactttttctaatgtttgagataaataaattttattagtgataagttagagaaaagcatgcggtcgtccgtgatataaaaacacataaaaaaccgaccgcacttttgcgaagagattta Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: - Start: 128 End: 232 ............................................................................................................................... H K L Y I F K I L S L N S F L M R D D T I Y F C M F F R G C K Q S S M ... Protein_Len: 31 Strand: - Start: 46 End: 138 ............................................. N Q Q N F C C S I V F H L T F K H G L D C I K I S K R I N S M ................................................................................................. attttctcctgttgtgtgtgaaataaccgctttaagattaacctaattttgctgattaaaacaacaagagatcacaaaatgaagggtaaatttgtgacctagatcacatattttgatactttttctaatgtttgagataaataaattttattagtgataagttagagaaaagcatgcggtcgtccgtgatataaaaacacataaaaaaccgaccgcacttttgcgaagagattta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ attttctcctgttgtgtgtgaaataaccgctttaagattaacctaattttgctgattaaaacaacaagagatcacaaaatgaagggtaaatttgtgacctagatcacatattttgatactttttctaatgtttgagataaataaattttattagtgataagttagagaaaagcatgcggtcgtccgtgatataaaaacacataaaaaaccgaccgcacttttgcgaagagattta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01845:AA01846|AA01845:AA01846:gamma-glutamyltranspeptidase precursor:conserved hypothetical protein:->->:1241748..1241982 235 attttctcctgttgtgtgtgaaataaccgctttaagattaacctaattttgctgattaaaacaacaagagatcacaaaatgaagggtaaatttgtgacctagatcacatattttgatactttttctaatgtttgagataaataaattttattagtgataagttagagaaaagcatgcggtcgtccgtgatataaaaacacataaaaaaccgaccgcacttttgcgaagagattta Intra-Species Hit: Count: 1 Min: 1 Max: 235 Len: 235 Subject: aact_AA01845_AA01846|gamma-glutamyltranspeptidase precursor:conserved hypothetical protein|POSITIVE:POSITIVE|[1241748,1241982]|235 HSP 1 e-value: 1.0E-130 bit: 466.0 Len: 235 Query Start:1 Query End:235 Subject Strand: POSITIVE Subject Start: 1 Subject End: 235 attttctcctgttgtgtgtgaaataaccgctttaagattaacctaattttgctgattaaaacaacaagagatcacaaaatgaagggtaaatttgtgacctagatcacatattttgatactttttctaatgtttgagataaataaattttattagtgataagttagagaaaagcatgcggtcgtccgtgatataaaaacacataaaaaaccgaccgcacttttgcgaagagattta attttctcctgttgtgtgtgaaataaccgctttaagattaacctaattttgctgattaaaacaacaagagatcacaaaatgaagggtaaatttgtgacctagatcacatattttgatactttttctaatgtttgagataaataaattttattagtgataagttagagaaaagcatgcggtcgtccgtgatataaaaacacataaaaaaccgaccgcacttttgcgaagagattta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUUUCUCCUGUUGUGUGUGAAAUAACCGCUUUAAGAUUAACCUAAUUUUGCUGAUUAAAACAACAAGAGAUCACAAAAUGAAGGGUAAAUUUGUGACCUAGAUCACAUAUUUUGAUACUUUUUCUAAUGUUUGAGAUAAAUAAAUUUUAUUAGUGAUAAGUUAGAGAAAAGCAUGCGGUCGUCCGUGAUAUAAAAACACAUAAAAAACCGACCGCACUUUUGCGAAGAGAUUUA ....((((.....((((((((..((..(((....(((((.((((.((((((.(((((............)))))))))))...)))).))))))))...))..))))))))........(((((((((((((...((((((.....))))))...)))..)))))))))).(((((((((((...........................))))))))....)))...)))).... (-47.63) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUCUCUUCGCAAAAGUGCGGUCGGUUUUUUAUGUGUUUUUAUAUCACGGACGACCGCAUGCUUUUCUCUAACUUAUCACUAAUAAAAUUUAUUUAUCUCAAACAUUAGAAAAAGUAUCAAAAUAUGUGAUCUAGGUCACAAAUUUACCCUUCAUUUUGUGAUCUCUUGUUGUUUUAAUCAGCAAAAUUAGGUUAAUCUUAAAGCGGUUAUUUCACACACAACAGGAGAAAAU .........((((((((..(((((((.((..(.(((((....))))).)..))))))))).(((((((.(((((..........(((((.....)))))........))))))))))))...((((.((((((....)))))).))))........))))))))(((((((((((..(((((.((....(((((.....))))).)))))))........))))))))))).... (-52.17) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 489 229 181 176 122 aact:AA01458|5end_aminotransferase_978820..979050_POSITIVE 455 224 181 199 158 aact:AA00268|5end_acyl_carrier_protein_(ACP)_188104..188334_POSITIVE 455 224 181 48 7 aact:AA00267|5end_hypothetical_protein_187953..188183_POSITIVE 438 220 171 149 93 aact:AA00324|5end_tRNA_(Uracil-5-)-methyltransferase_(tRNA(M-5-U54)-methyltransferase)_220147..220377_POSITIVE 431 230 181 138 84 aact:AA01832|5end_formate_dehydrogenase_formation_protein_E_1233609..1233839_POSITIVE 429 230 192 145 97 aact:AA01436|5end_fructose-bisphosphate_aldolase_class_II_962981..963211_POSITIVE 393 220 175 163 115 aact:AA02249|5end_thiamine_biosynthesis_protein_1528365..1528595_POSITIVE 390 220 175 173 113 aact:AA00819|5end_D-alanyl-D-alanine_carboxypeptidase_562644..562874_POSITIVE 369 220 175 182 130 aact:AA01824|5end_iron(III)_ABC_transporter,_ATP-binding_protein_1227705..1227935_POSITIVE 367 230 181 174 120 aact:AA00130|5end_mercuric_transport_protein_(periplasmic_mercury_ion_binding_protein)_(mercury_scavenger_protein)_94217..94447_POSITIVE 367 230 181 79 25 aact:AA00129|5end_hypothetical_protein_94122..94352_POSITIVE 365 220 171 123 72 aact:AA02716|5end_peptidase_T_1899789..1900019_POSITIVE 364 220 174 121 74 aact:AA02276|5end_pseudouridine_synthase_Rlu_family_1549932..1550162_POSITIVE 353 224 181 126 84 aact:AA01936|5end_tyrosyl-tRNA_synthetase_1307043..1307273_POSITIVE 353 220 175 210 152 aact:AA00515|5end_hypothetical_protein_355730..355960_POSITIVE 353 220 175 89 31 aact:AA00514|5end_hypothetical_protein_355609..355839_POSITIVE 347 228 184 79 36 aact:AA02589|5end_NAD(P)H_oxidoreductase_1800493..1800723_POSITIVE 346 220 175 155 98 aact:AA02097|5end_DedA-family_integral_membrane_protein_1428823..1429053_POSITIVE 346 220 175 86 29 aact:AA02096|5end_hypothetical_protein_1428754..1428984_POSITIVE 345 230 184 103 60 aact:AA01641|5end_hypothetical_protein_1110819..1111049_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 489 229 181 84 30 aact:AA01457|3end_D-xylose_ABC_transporter,_membrane_spanning_protein_978808..978958_POSITIVE 438 220 171 144 88 aact:AA00323|3end_conserved_hypothetical_protein_(possible_SAM-dependent_methyltransferase)_220222..220372_POSITIVE 431 230 181 58 4 aact:AA01830|3end_2,3-cyclic-nucleotide_2-phosphodiesterase_1233609..1233759_POSITIVE 429 230 192 105 57 aact:AA01434|3end_conserved_hypothetical_protein_(possible_o-antigen_ligase)_963021..963171_POSITIVE 424 220 181 151 117 aact:AA00267|3end_hypothetical_protein_188143..188293_POSITIVE 393 220 175 73 25 aact:AA02246|3end_peptidase_E_1528355..1528505_POSITIVE 369 220 175 144 92 aact:AA01823|3end_hypothetical_protein_1227747..1227897_POSITIVE 369 220 175 68 16 aact:AA01822|3end_lysostaphin_precursor_1227671..1227821_POSITIVE 367 230 181 84 30 aact:AA00128|3end_cation-transporting_ATPase_(probable_potassium/copper_transporter)_94207..94357_POSITIVE 364 220 174 64 17 aact:AA02275|3end_conserved_hypothetical_protein_1549955..1550105_POSITIVE 353 220 175 86 28 aact:AA00513|3end_pseudouridylate_synthase_(pseudouridine_synthase)_355686..355836_POSITIVE 347 228 184 76 33 aact:AA02587|3end_conserved_hypothetical_protein_1800570..1800720_POSITIVE 340 225 183 58 19 aact:AA02964|3end_opacity_associated_protein_A_2074128..2074278_POSITIVE 338 220 171 111 47 aact:AA01185|3end_conserved_hypothetical_protein_(possible_membrane_protein)_813401..813551_POSITIVE 337 222 181 81 43 aact:AA01459|3end_Na(+)/H(+)_antiporter_981492..981642_POSITIVE 337 220 179 121 79 aact:AA01044|3end_A/G-specific_adenine_glycosylase_727978..728128_POSITIVE 337 230 189 45 2 aact:AA00612|3end_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosami_ne_418705..418855_POSITIVE 336 227 186 112 68 aact:AA00732|3end_citrate_lyase_ligase_502427..502577_POSITIVE 330 230 193 73 33 aact:AA01945|3end_selenophosphate_synthase_1316022..1316172_POSITIVE 330 220 175 41 1 aact:AA00764|3end_hemoglobin_binding_protein_A_527347..527497_POSITIVE