Origin IGS: tagagactaaaaaggatcaacgactgatcctttaatttctgtattaactatctaattcggaaagtcagcagtaaaacgccgagggttgtgggaccgtggttgatttgaatttaacgacgctatcagttcgattatcttctataaagcgtatatcgagatattccgtatcatataaaaatacctgcccgtgtccattgggcatgctatattgagacccgtcattaacaaagtcggcttgtagttttacgaccactaatgctaattcagccatgatttcttgttcagttctcatttttaatgtagtcatttttatttcctgtgttttaaactgattacgggattactctgctcaattttgtaagagcgtttcactatgtgcaataatcttaattttaatttatcgcgctgagcctagttaatttatcgcgcgcggcatcagctgacccgcgattttattgaagacaaactttataactgggataacgaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttcgttatcccagttataaagtttgtcttcaataaaatcgcgggtcagctgatgccgcgcgcgataaattaactaggctcagcgcgataaattaaaattaagattattgcacatagtgaaacgctcttacaaaattgagcagagtaatcccgtaatcagtttaaaacacaggaaataaaaatgactacattaaaaatgagaactgaacaagaaatcatggctgaattagcattagtggtcgtaaaactacaagccgactttgttaatgacgggtctcaatatagcatgcccaatggacacgggcaggtatttttatatgatacggaatatctcgatatacgctttatagaagataatcgaactgatagcgtcgttaaattcaaatcaaccacggtcccacaaccctcggcgttttactgctgactttccgaattagatagttaatacagaaattaaaggatcagtcgttgatcctttttagtctcta Mask Tandem Repeat Region ================================================ tagagactaaaaaggatcaacgactgatcctttaatttctgtattaactatctaattcggaaagtcagcagtaaaacgccgagggttgtgggaccgtggttgatttgaatttaacgacgctatcagttcgattatcttctataaagcgtatatcgagatattccgtatcatataaaaatacctgcccgtgtccattgggcatgctatattgagacccgtcattaacaaagtcggcttgtagttttacgaccactaatgctaattcagccatgatttcttgttcagttctcatttttaatgtagtcatttttatttcctgtgttttaaactgattacgggattactctgctcaattttgtaagagcgtttcactatgtgcaataatcttaattttaatttatcgcgctgagcctagttaatttatcgcgcgcggcatcagctgacccgcgattttattgaagacaaactttataactgggataacgaa Find is-nt database================================================ Query_seq: AA01841:AA01842|AA01841:AA01842:conserved hypothetical protein:conserved hypothetical protein:->->:1237924..1238410 487 tagagactaaaaaggatcaacgactgatcctttaatttctgtattaactatctaattcggaaagtcagcagtaaaacgccgagggttgtgggaccgtggttgatttgaatttaacgacgctatcagttcgattatcttctataaagcgtatatcgagatattccgtatcatataaaaatacctgcccgtgtccattgggcatgctatattgagacccgtcattaacaaagtcggcttgtagttttacgaccactaatgctaattcagccatgatttcttgttcagttctcatttttaatgtagtcatttttatttcctgtgttttaaactgattacgggattactctgctcaattttgtaagagcgtttcactatgtgcaataatcttaattttaatttatcgcgctgagcctagttaatttatcgcgcgcggcatcagctgacccgcgattttattgaagacaaactttataactgggataacgaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01841:AA01842|AA01841:AA01842:conserved hypothetical protein:conserved hypothetical protein:->->:1237924..1238410 487 tagagactaaaaaggatcaacgactgatcctttaatttctgtattaactatctaattcggaaagtcagcagtaaaacgccgagggttgtgggaccgtggttgatttgaatttaacgacgctatcagttcgattatcttctataaagcgtatatcgagatattccgtatcatataaaaatacctgcccgtgtccattgggcatgctatattgagacccgtcattaacaaagtcggcttgtagttttacgaccactaatgctaattcagccatgatttcttgttcagttctcatttttaatgtagtcatttttatttcctgtgttttaaactgattacgggattactctgctcaattttgtaagagcgtttcactatgtgcaataatcttaattttaatttatcgcgctgagcctagttaatttatcgcgcgcggcatcagctgacccgcgattttattgaagacaaactttataactgggataacgaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01841:AA01842|AA01841:AA01842:conserved hypothetical protein:conserved hypothetical protein:->->:1237924..1238410 487 tagagactaaaaaggatcaacgactgatcctttaatttctgtattaactatctaattcggaaagtcagcagtaaaacgccgagggttgtgggaccgtggttgatttgaatttaacgacgctatcagttcgattatcttctataaagcgtatatcgagatattccgtatcatataaaaatacctgcccgtgtccattgggcatgctatattgagacccgtcattaacaaagtcggcttgtagttttacgaccactaatgctaattcagccatgatttcttgttcagttctcatttttaatgtagtcatttttatttcctgtgttttaaactgattacgggattactctgctcaattttgtaagagcgtttcactatgtgcaataatcttaattttaatttatcgcgctgagcctagttaatttatcgcgcgcggcatcagctgacccgcgattttattgaagacaaactttataactgggataacgaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 50 Max: 307 Len: 258 Subject: UniRef90_Q4QKT4 Cluster: Hypothetical protein; n=1; Haemophilus influenzae 86-028NP|Rep: Hypothetical protein - Haemophilus influenzae (strain 86-028NP) HSP 1 e-value: 2.0E-21 bit: 103.0 Len: 258 Query Start:50 Query End:307 Subject Strand: null Subject Start: 1 Subject End: 86 ................................................. M T T L K M R T E Q E I M A E L A L V V V K L Q A D F V N D G S Q Y S M P N G H G Q V F L Y D T E Y L D I R F I E D N R T D S V V K F K S T T V P Q P S A F Y C * L S E L D .................................................................................................................................................................................... ................................................. M T N F Q M R T E Q E M M M E L A L I A V K E H D G F I V D G S Q Y S L P N E C G Q T F V Y N T A F L D V R F V E D G R K D C V V R F T S T L A P F A P P F Y C L L S E L E .................................................................................................................................................................................... tagagactaaaaaggatcaacgactgatcctttaatttctgtattaactnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttttatttcctgtgttttaaactgattacgggattactctgctcaattttgtaagagcgtttcactatgtgcaataatcttaattttaatttatcgcgctgagcctagttaatttatcgcgcgcggcatcagctgacccgcgattttattgaagacaaactttataactgggataacgaa Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: + Start: 96 End: 209 ............................................................................................... M V D L N L T T L S V R L S S I K R I S R Y S V S Y K N T C P C P L G M L Y ...................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 68 End: 313 ................................................................... D E I F R I D L Y E T D Y L F V Q G H G N P M S Y Q S G D N V F D A Q L K V V V L A L E A M I E Q E T R M K L T T M K M .............................................................................................................................................................................. tagagactaaaaaggatcaacgactgatcctttaatttctgtattaactnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttttatttcctgtgttttaaactgattacgggattactctgctcaattttgtaagagcgtttcactatgtgcaataatcttaattttaatttatcgcgctgagcctagttaatttatcgcgcgcggcatcagctgacccgcgattttattgaagacaaactttataactgggataacgaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagagactaaaaaggatcaacgactgatcctttaatttctgtattaactnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttttatttcctgtgttttaaactgattacgggattactctgctcaattttgtaagagcgtttcactatgtgcaataatcttaattttaatttatcgcgctgagcctagttaatttatcgcgcgcggcatcagctgacccgcgattttattgaagacaaactttataactgggataacgaa Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 77 HP_score: -6.5 Tail_Score: -4.63218 Start: 424 End: 450 Full_Region: AGCCTAGTTAATTTA TCGCGCGCGGC ATCA GCTGACCCGCGA TTTTATTGAAGACAA .......................................................................................................................................................................................................................................................................................................................................................................................................................................TCGCGCGCGGCATCAGCTGACCCGCGA..................................... Find igs database================================================ Query_seq: AA01841:AA01842|AA01841:AA01842:conserved hypothetical protein:conserved hypothetical protein:->->:1237924..1238410 487 tagagactaaaaaggatcaacgactgatcctttaatttctgtattaactnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttttatttcctgtgttttaaactgattacgggattactctgctcaattttgtaagagcgtttcactatgtgcaataatcttaattttaatttatcgcgctgagcctagttaatttatcgcgcgcggcatcagctgacccgcgattttattgaagacaaactttataactgggataacgaa Intra-Species Hit: Count: 1 Min: 1 Max: 487 Len: 487 Subject: aact_AA01841_AA01842|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1237924,1238410]|487 HSP 1 e-value: 2.0E-97 bit: 357.0 Len: 180 Query Start:308 Query End:487 Subject Strand: POSITIVE Subject Start: 308 Subject End: 487 ...................................................................................................................................................................................................................................................................................................................ttttatttcctgtgttttaaactgattacgggattactctgctcaattttgtaagagcgtttcactatgtgcaataatcttaattttaatttatcgcgctgagcctagttaatttatcgcgcgcggcatcagctgacccgcgattttattgaagacaaactttataactgggataacgaa ...................................................................................................................................................................................................................................................................................................................ttttatttcctgtgttttaaactgattacgggattactctgctcaattttgtaagagcgtttcactatgtgcaataatcttaattttaatttatcgcgctgagcctagttaatttatcgcgcgcggcatcagctgacccgcgattttattgaagacaaactttataactgggataacgaa HSP 2 e-value: 4.0E-19 bit: 97.6 Len: 49 Query Start:1 Query End:49 Subject Strand: POSITIVE Subject Start: 1 Subject End: 49 tagagactaaaaaggatcaacgactgatcctttaatttctgtattaact...................................................................................................................................................................................................................................................................................................................................................................................................................................................... tagagactaaaaaggatcaacgactgatcctttaatttctgtattaact...................................................................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAGACUAAAAAGGAUCAACGACUGAUCCUUUAAUUUCUGUAUUAACUAUCUAAUUCGGAAAGUCAGCAGUAAAACGCCGAGGGUUGUGGGACCGUGGUUGAUUUGAAUUUAACGACGCUAUCAGUUCGAUUAUCUUCUAUAAAGCGUAUAUCGAGAUAUUCCGUAUCAUAUAAAAAUACCUGCCCGUGUCCAUUGGGCAUGCUAUAUUGAGACCCGUCAUUAACAAAGUCGGCUUGUAGUUUUACGACCACUAAUGCUAAUUCAGCCAUGAUUUCUUGUUCAGUUCUCAUUUUUAAUGUAGUCAUUUUUAUUUCCUGUGUUUUAAACUGAUUACGGGAUUACUCUGCUCAAUUUUGUAAGAGCGUUUCACUAUGUGCAAUAAUCUUAAUUUUAAUUUAUCGCGCUGAGCCUAGUUAAUUUAUCGCGCGCGGCAUCAGCUGACCCGCGAUUUUAUUGAAGACAAACUUUAUAACUGGGAUAACGAA ....((((..(((((((((.....)))))))))...(((((.((((......)))).)))))))))...(((.((((((.((((((.((.(((((((.(((((.......))))).)))......)))).)).)))))).....((((......(((..(((((((((((....(((((((.((((((.......)))))).((((((((((((......((((((((((((((((..((((.(((.......))).))))....))))..))))..))))).)))......))))))))))))..............)))))))....))).))))))))..))))))).............)))))).)))..((((.((((.............)))).))))....((((((((....((((((.(((((....)))).).)))))).....(((((.....)))))))))))))........ (-120.62) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUCGUUAUCCCAGUUAUAAAGUUUGUCUUCAAUAAAAUCGCGGGUCAGCUGAUGCCGCGCGCGAUAAAUUAACUAGGCUCAGCGCGAUAAAUUAAAAUUAAGAUUAUUGCACAUAGUGAAACGCUCUUACAAAAUUGAGCAGAGUAAUCCCGUAAUCAGUUUAAAACACAGGAAAUAAAAAUGACUACAUUAAAAAUGAGAACUGAACAAGAAAUCAUGGCUGAAUUAGCAUUAGUGGUCGUAAAACUACAAGCCGACUUUGUUAAUGACGGGUCUCAAUAUAGCAUGCCCAAUGGACACGGGCAGGUAUUUUUAUAUGAUACGGAAUAUCUCGAUAUACGCUUUAUAGAAGAUAAUCGAACUGAUAGCGUCGUUAAAUUCAAAUCAACCACGGUCCCACAACCCUCGGCGUUUUACUGCUGACUUUCCGAAUUAGAUAGUUAAUACAGAAAUUAAAGGAUCAGUCGUUGAUCCUUUUUAGUCUCUA ..(((((((..((((.....(.((((((((.(((((..((((((((....))).)))))((.((((.....(((..((((((.(((((((.(((....)))..))))))).....((((.......))))....))))))..)))....(((((.(((...(((((..............................(((((.(((..((.(....).)).....(((((((..((((((.(((....)))..))).))).)))))))..))).))))).....((.(((((.........))))).))..)))))..))))))))..)))).))........))))).)))))))).).)))))))))))..(((.(((((...........(((......))).((((((......))))))......))))).))).........(((....((((((((((...))))))))))....)))... (-111.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 628 438 396 165 123 aact:AA02007|5end_hypothetical_protein_1365748..1365978_POSITIVE 407 450 402 63 2 aact:AA01982|5end_maltodextrin_phosphorylase_1339387..1339617_POSITIVE 407 462 424 57 16 aact:AA00286|5end_glutamate_5-kinase_(gamma-glutamyl_kinase)_199965..200195_POSITIVE 379 450 405 72 25 aact:AA02756|5end_phosphoserine_aminotransferase_1929477..1929707_POSITIVE 372 449 401 229 177 aact:AA00101|5end_fimbrial_biogenesis_and_twitching_motility_protein_73782..74012_POSITIVE 368 450 402 202 141 aact:AA02439|5end_integral_membrane_protein;_possible_ABC_transporter_permease_1683902..1684132_POSITIVE 367 443 407 154 110 aact:AA01367|5end_tRNA_(Guanine-N(1)-)-methyltransferase_(M1G-methyltransferase)_923710..923940_POSITIVE 367 450 403 122 63 aact:AA01274|5end_hypothetical_protein_864519..864749_POSITIVE 367 450 403 137 78 aact:AA00333|5end_1-phosphofructokinase;_tagatose-6-phosphate_kinase_225543..225773_POSITIVE 366 449 403 166 126 aact:AA00381|5end_ornithine_carbamoyltransferase_257470..257700_POSITIVE 364 219 180 181 143 aact:AA01857|5end_glutamate-ammonia-ligase_adenylyltransferase_1247347..1247577_POSITIVE 362 449 403 201 150 aact:AA00335|5end_PTS_fructose-specific_IIBC_component_226489..226719_POSITIVE 360 450 403 221 166 aact:AA02047|5end_galactitol-1-phosphate_5-dehydrogenase_1393633..1393863_POSITIVE 358 467 426 134 76 aact:AA01276|5end_heptosyltransferase_III_864730..864960_POSITIVE 352 462 423 160 120 aact:AA01119|5end_hypothetical_protein_769291..769521_POSITIVE 350 448 402 99 52 aact:AA00240|5end_hypothetical_protein_171390..171620_POSITIVE 347 458 426 154 120 aact:AA01417|5end_probable_4-diphosphocytidyl-2C-methyl-D-erthyritol_synthase_(MEP_cytidyltransferase)_(MCT)_953256..953486_POSITIVE 347 435 399 157 121 aact:AA00798|5end_iron(III)_dicitrate_transport_system_permease_protein_546675..546905_POSITIVE 344 220 181 199 154 aact:AA02696|5end_formate_hydrogenlyase_maturation_protein_1879476..1879706_POSITIVE 344 448 403 141 93 aact:AA00735|5end_citrate_lyase,_beta_chain_502674..502904_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 367 443 407 85 41 aact:AA01366|3end_16S_rRNA_processing_protein_923721..923871_POSITIVE 367 450 403 111 52 aact:AA01273|3end_hypothetical_protein_864588..864738_POSITIVE 367 450 403 131 72 aact:AA00332|3end_PTS_system,_fructose-specific_IIA/FPr_component_(diphosphoryl_transfer_protein)_225617..225767_POSITIVE 364 219 180 139 101 aact:AA01855|3end_transcriptional_regulator,_Cro/CI_family_1247385..1247535_POSITIVE 356 450 403 139 85 aact:AA00602|3end_1-deoxy-D-xylulose_5-phosphate_reductoisomerase_410251..410401_POSITIVE 344 220 181 61 16 aact:AA02695|3end_hydrogenase-3_component_G_1879418..1879568_POSITIVE 344 448 403 141 93 aact:AA00734|3end_citrate_lyase,_gamma_chain_(acyl_carrier_protein)_502754..502904_POSITIVE 343 449 402 64 25 aact:AA02952|3end_lipoprotein_signal_peptidase_2065687..2065837_POSITIVE 342 450 401 65 27 aact:AA02225|3end_ABC_transporter_ATP-binding_protein_1514592..1514742_POSITIVE 339 448 402 145 97 aact:AA01416|3end_2C-methyl-D-erythritol_2,4-cyclodiphosphate_953336..953486_POSITIVE 333 450 403 73 14 aact:AA02525|3end_conserved_hypothetical_protein_1755302..1755452_POSITIVE 333 450 403 65 30 aact:AA00474|3end_cell_division_protein_Y_(GTP-binding_signal_recognition_particle)_331724..331874_POSITIVE 330 449 403 105 50 aact:AA01616|3end_lipoprotein_releasing_system_ATP-binding_protein_1093148..1093298_POSITIVE 326 450 403 86 32 aact:AA00211|3end_hypothetical_protein_146163..146313_POSITIVE 325 450 402 59 18 aact:AA01981|3end_4-alpha-glucanotransferase_1339354..1339504_POSITIVE 324 99 56 72 27 aact:AA02772|3end_hypothetical_protein_1936776..1936926_POSITIVE 324 447 401 54 7 aact:AA01772|3end_shikimate_5-dehydrogenase_protein_1194942..1195092_POSITIVE 324 445 402 114 63 aact:AA01660|3end_hypothetical_protein_1125282..1125432_POSITIVE 321 444 403 149 113 aact:AA00380|3end_carbamate_kinase_257537..257687_POSITIVE 319 449 401 57 2 aact:AA01108|3end_hypothetical_protein_763983..764133_POSITIVE