Origin IGS: agttgtgttgttaatagaaagtgaaaattcgtgcttattataggcgcataaatttgccgttcaatgaccctaacgggtattttatttgttcgccagccattgcttaatgcgattta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaatcgcattaagcaatggctggcgaacaaataaaatacccgttagggtcattgaacggcaaatttatgcgcctataataagcacgaattttcactttctattaacaacacaact Mask Tandem Repeat Region ================================================ agttgtgttgttaatagaaagtgaaaattcgtgcttattataggcgcataaatttgccgttcaatgaccctaacgggtattttatttgttcgccagccattgcttaatgcgattta Find is-nt database================================================ Query_seq: AA01832:AA01834|AA01832:AA01834:formate dehydrogenase formation protein E:hypothetical protein:->->:1234664..1234779 116 agttgtgttgttaatagaaagtgaaaattcgtgcttattataggcgcataaatttgccgttcaatgaccctaacgggtattttatttgttcgccagccattgcttaatgcgattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01832:AA01834|AA01832:AA01834:formate dehydrogenase formation protein E:hypothetical protein:->->:1234664..1234779 116 agttgtgttgttaatagaaagtgaaaattcgtgcttattataggcgcataaatttgccgttcaatgaccctaacgggtattttatttgttcgccagccattgcttaatgcgattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01832:AA01834|AA01832:AA01834:formate dehydrogenase formation protein E:hypothetical protein:->->:1234664..1234779 116 agttgtgttgttaatagaaagtgaaaattcgtgcttattataggcgcataaatttgccgttcaatgaccctaacgggtattttatttgttcgccagccattgcttaatgcgattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agttgtgttgttaatagaaagtgaaaattcgtgcttattataggcgcataaatttgccgttcaatgaccctaacgggtattttatttgttcgccagccattgcttaatgcgattta Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: - Start: 3 End: 113 .. Q T T L L F H F N T S I I P A Y I Q R E I V R V P I K N T R W G N S L A M ... agttgtgttgttaatagaaagtgaaaattcgtgcttattataggcgcataaatttgccgttcaatgaccctaacgggtattttatttgttcgccagccattgcttaatgcgattta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agttgtgttgttaatagaaagtgaaaattcgtgcttattataggcgcataaatttgccgttcaatgaccctaacgggtattttatttgttcgccagccattgcttaatgcgattta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01832:AA01834|AA01832:AA01834:formate dehydrogenase formation protein E:hypothetical protein:->->:1234664..1234779 116 agttgtgttgttaatagaaagtgaaaattcgtgcttattataggcgcataaatttgccgttcaatgaccctaacgggtattttatttgttcgccagccattgcttaatgcgattta Intra-Species Hit: Count: 1 Min: 1 Max: 116 Len: 116 Subject: aact_AA01832_AA01834|formate dehydrogenase formation protein E:hypothetical protein|POSITIVE:POSITIVE|[1234664,1234779]|116 HSP 1 e-value: 8.0E-60 bit: 230.0 Len: 116 Query Start:1 Query End:116 Subject Strand: POSITIVE Subject Start: 1 Subject End: 116 agttgtgttgttaatagaaagtgaaaattcgtgcttattataggcgcataaatttgccgttcaatgaccctaacgggtattttatttgttcgccagccattgcttaatgcgattta agttgtgttgttaatagaaagtgaaaattcgtgcttattataggcgcataaatttgccgttcaatgaccctaacgggtattttatttgttcgccagccattgcttaatgcgattta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUUGUGUUGUUAAUAGAAAGUGAAAAUUCGUGCUUAUUAUAGGCGCAUAAAUUUGCCGUUCAAUGACCCUAACGGGUAUUUUAUUUGUUCGCCAGCCAUUGCUUAAUGCGAUUUA ((((((((((.((((.(...(((((.....(((((((...)))))))...................((((....))))..........)))))...).))))..)))))))))).. (-25.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUCGCAUUAAGCAAUGGCUGGCGAACAAAUAAAAUACCCGUUAGGGUCAUUGAACGGCAAAUUUAUGCGCCUAUAAUAAGCACGAAUUUUCACUUUCUAUUAACAACACAACU ....((((....(((....))).))))...........((((....))))....(((.((.(((((..(((...........)))..))))).).).)))................ (-20.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 373 69 26 51 5 aact:AA01141|5end_conserved_hypothetical_protein_(possible_component_of_anaerobic_dehydrogenase/oxidoreductase)_782497..782727_POSITIVE 337 110 63 64 19 aact:AA01615|5end_lipoprotein_releasing_system_transmembrane_1091186..1091416_POSITIVE 303 86 43 52 9 aact:AA01299|5end_competence_protein_C_880946..881176_POSITIVE 300 99 58 231 185 aact:AA01981|5end_4-alpha-glucanotransferase_1337202..1337432_POSITIVE 300 99 58 84 38 aact:AA01978|5end_hypothetical_protein_1337055..1337285_POSITIVE 300 99 57 231 187 aact:AA00148|5end_ATP-dependent_RNA_helicase_105955..106185_POSITIVE 297 98 56 85 36 aact:AA01996|5end_prephenate_dehydrogenase_1355314..1355544_POSITIVE 296 97 54 207 168 aact:AA02980|5end_conserved_hypothetical_protein_2083305..2083535_POSITIVE 289 98 51 164 114 aact:AA01056|5end_conserved_hypothetical_protein_733867..734097_POSITIVE 288 83 43 211 167 aact:AA00737|5end_citrate_lyase,_alpha_chain_(citrase)_503564..503794_POSITIVE 284 98 53 212 167 aact:AA02575|5end_transmembrane_protein_1793749..1793979_POSITIVE 283 98 57 149 107 aact:AA02088|5end_glycogen_operon_protein_1421282..1421512_POSITIVE 283 88 43 231 193 aact:AA00962|5end_exopolyphosphatase_(metaphosphatase)_671060..671290_POSITIVE 281 78 43 72 36 aact:AA02206|5end_tryptophan_synthase,_beta_chain_1501917..1502147_POSITIVE 277 96 53 132 81 aact:AA00373|5end_CoA-transferase_(beta)_251013..251243_POSITIVE 276 87 42 171 134 aact:AA00680|5end_sodium/proline_symporter_(proline_permease)_464899..465129_POSITIVE 273 56 11 152 114 aact:AA01868|5end_biotin_synthetase_1255545..1255775_POSITIVE 272 83 43 37 12 aact:AA00106|5end_1-hydroxy-2-methyl-2-(E)-butenyl_4-diphosphate_synthase_75544..75774_POSITIVE 271 98 51 230 186 aact:AA00091|5end_short-patch_reverse_transcription_protein_67741..67971_POSITIVE 270 77 33 120 83 aact:AA00492|5end_conserved_hypothetical_protein_343578..343808_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 303 86 43 49 6 aact:AA01298|3end_competence_protein_D_881023..881173_POSITIVE 300 99 58 81 35 aact:AA01976|3end_colicin_V_production_protein_1337132..1337282_POSITIVE 300 99 57 140 96 aact:AA00146|3end_UDP-glucose/GDP-mannose_dehydrogenase_105944..106094_POSITIVE 297 98 56 85 36 aact:AA01995|3end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355394..1355544_POSITIVE 296 97 54 130 91 aact:AA02978|3end_sufI_protein_precursor_homolog_2083308..2083458_POSITIVE 281 78 43 59 23 aact:AA02205|3end_short_chain_dehydrogenase/reductase_1501984..1502134_POSITIVE 277 96 53 117 66 aact:AA00372|3end_acetate_CoA-transferase_(alpha_subunit)_251078..251228_POSITIVE 276 103 65 151 110 aact:AA02619|3end_GTP_cyclohydrolase_II_1826193..1826343_POSITIVE 273 56 11 104 66 aact:AA01867|3end_thiamine_transport_ATP-binding_protein_1255577..1255727_POSITIVE 270 78 43 137 93 aact:AA01054|3end_conserved_hypothetical_protein_733940..734090_POSITIVE 262 103 66 48 12 aact:AA01947|3end_iron(III)-transport_ATP-binding_protein_1317330..1317480_POSITIVE 261 82 43 143 112 aact:AA01400|3end_phosphopantetheine_adenylyltransferase_946241..946391_POSITIVE 257 103 75 129 91 aact:AA02663|3end_DNA_ligase_1854351..1854501_POSITIVE 254 105 68 86 47 aact:AA02230|3end_sugar_kinase_1517617..1517767_POSITIVE 253 110 61 146 104 aact:AA02105|3end_killing_protein_1433290..1433440_POSITIVE 252 110 85 94 65 aact:AA02260|3end_pseudouridylate_synthase_I_1537962..1538112_POSITIVE 252 110 67 139 94 aact:AA02139|3end_possible_membrane_protein_1462218..1462368_POSITIVE 248 104 75 113 79 aact:AA02990|3end_beta_hexosaminadase_2090596..2090746_POSITIVE 248 78 31 56 17 aact:AA02958|3end_3-phenylpropionic_acid_transporter_2070491..2070641_POSITIVE 248 110 61 146 86 aact:AA00506|3end_ATP-dependent_RNA_helicase_protein_353089..353239_POSITIVE